MAGED1 (NM_006986) Human Tagged ORF Clone

CAT#: RC214851

MAGED1 (Myc-DDK-tagged)-Human melanoma antigen family D, 1 (MAGED1), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY416269) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214851 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MAGED1
• Synonyms: DLXIN-1; NRAGE
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214851 representing NM_006986
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCAGAAAATGGACTGTGGTGCGGGCCTCCTCGGCTTCCAGGCTGAGGCCTCCGTAGAAGACAGCG
  CCTTGCTTATGCAGACCTTGATGGAGGCCATCCAGATCTCAGAGGCTCCACCTACTAACCAGGCCACCGC
  AGCTGCTAGTCCCCAGAGTTCACAGCCCCCAACTGCCAATGAGATGGCTGACATTCAGGTTTCAGCAGCT
  GCCGCTAGGCCTAAGTCAGCCTTTAAAGTCCAGAATGCCACCACAAAAGGCCCAAATGGTGTCTATGATT
  TCTCTCAGGCTCATAATGCCAAGGATGTGCCCAACACGCAGCCCAAGGCAGCCTTTAAGTCCCAAAATGC
  TACCCCAAAGGGTCCAAATGCTGCCTATGATTTTTCCCAGGCAGCAACCACTGGTGAGTTAGCTGCTAAC
  AAGTCTGAGATGGCCTTCAAGGCCCAGAATGCCACTACTAAAGTGGGCCCAAATGCCACCTACAATTTCT
  CTCAGTCTCTCAATGCCAATGACCTGGCCAACAGCAGGCCTAAGACCCCTTTCAAGGCTTGGAATGATAC
  CACTAAGGCCCCAACAGCTGATACCCAGACCCAGAATGTAAATCAGGCCAAAATGGCCACTTCCCAGGCT
  GACATAGAGACCGACCCAGGTATCTCTGAACCTGACGGTGCAACTGCACAGACATCAGCAGATGGTTCCC
  AGGCTCAGAATCTGGAGTCCCGGACAATAATTCGGGGCAAGAGGACCCGCAAGATTAATAACTTGAATGT
  TGAAGAGAACAGCAGTGGGGATCAGAGGCGGGCCCCACTGGCTGCAGGGACCTGGAGGTCTGCACCAGTT
  CCAGTGACCACTCAGAACCCACCTGGCGCACCCCCCAATGTGCTCTGGCAGACGCCATTGGCTTGGCAGA
  ACCCCTCAGGCTGGCAAAACCAGACAGCCAGGCAGACCCCACCAGCACGTCAGAGCCCTCCAGCTAGGCA
  GACCCCACCAGCCTGGCAGAACCCAGTCGCTTGGCAGAACCCAGTGATTTGGCCAAACCCAGTAATCTGG
  CAGAACCCAGTGATCTGGCCAAACCCCATTGTCTGGCCCGGCCCTGTTGTCTGGCCGAATCCACTGGCCT
  GGCAGAATCCACCTGGATGGCAGACTCCACCTGGATGGCAGACCCCACCGGGCTGGCAGGGTCCTCCAGA
  CTGGCAAGGTCCTCCTGACTGGCCGCTACCACCCGACTGGCCACTGCCACCTGATTGGCCACTTCCCACT
  GACTGGCCACTACCACCTGACTGGATCCCCGCTGATTGGCCAATTCCACCTGACTGGCAGAACCTGCGCC
  CCTCGCCTAACCTGCGCCCTTCTCCCAACTCGCGTGCCTCACAGAACCCAGGTGCTGCACAGCCCCGAGA
  TGTGGCCCTTCTTCAGGAAAGAGCAAATAAGTTGGTCAAGTACTTGATGCTTAAGGACTACACAAAGGTG
  CCCATCAAGCGCTCAGAAATGCTGAGAGATATCATCCGTGAATACACTGATGTTTATCCAGAAATCATTG
  AACGTGCATGCTTTGTCCTAGAGAAGAAATTTGGGATTCAACTGAAAGAAATTGACAAAGAAGAACACCT
  GTATATTCTCATCAGTACCCCCGAGTCCCTGGCTGGCATACTGGGAACGACCAAAGACACACCCAAGCTC
  GGTCTCCTCTTGGTGATTCTGGGTGTCATCTTCATGAATGGCAACCGTGCCAGTGAGGCTGTCCTCTGGG
  AGGCACTACGCAAGATGGGACTGCGTCCTGGGGTGAGACATCCCCTCCTTGGAGATCTAAGGAAACTTCT
  CACCTATGAGTTTGTAAAGCAGAAATACCTGGACTACAGACGAGTGCCCAACAGCAACCCCCCGGAGTAT
  GAGTTCCTCTGGGGCCTCCGTTCCTACCATGAGACTAGCAAGATGAAAGTGCTGAGATTCATTGCAGAGG
  TTCAGAAAAGAGACCCTCGTGACTGGACTGCACAGTTCATGGAGGCTGCAGATGAGGCCTTGGATGCTCT
  GGATGCTGCTGCAGCTGAGGCCGAAGCCCGGGCTGAAGCAAGAACCCGCATGGGAATTGGAGATGAGGCT
  GTGTCTGGGCCCTGGAGCTGGGATGACATTGAGTTTGAGCTGCTGACCTGGGATGAGGAAGGAGATTTTG
  GAGATCCCTGGTCCAGAATTCCATTTACCTTCTGGGCCAGATACCACCAGAATGCCCGCTCCAGATTCCC
  TCAGACCTTTGCCGGTCCCATTATTGGTCCTGGTGGTACAGCCAGTGCCAACTTCGCTGCCAACTTTGGT
  GCCATTGGTTTCTTCTGGGTTGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214851 representing NM_006986
  Red=Cloning site Green=Tags(s)
  
  MAQKMDCGAGLLGFQAEASVEDSALLMQTLMEAIQISEAPPTNQATAAASPQSSQPPTANEMADIQVSAA
  AARPKSAFKVQNATTKGPNGVYDFSQAHNAKDVPNTQPKAAFKSQNATPKGPNAAYDFSQAATTGELAAN
  KSEMAFKAQNATTKVGPNATYNFSQSLNANDLANSRPKTPFKAWNDTTKAPTADTQTQNVNQAKMATSQA
  DIETDPGISEPDGATAQTSADGSQAQNLESRTIIRGKRTRKINNLNVEENSSGDQRRAPLAAGTWRSAPV
  PVTTQNPPGAPPNVLWQTPLAWQNPSGWQNQTARQTPPARQSPPARQTPPAWQNPVAWQNPVIWPNPVIW
  QNPVIWPNPIVWPGPVVWPNPLAWQNPPGWQTPPGWQTPPGWQGPPDWQGPPDWPLPPDWPLPPDWPLPT
  DWPLPPDWIPADWPIPPDWQNLRPSPNLRPSPNSRASQNPGAAQPRDVALLQERANKLVKYLMLKDYTKV
  PIKRSEMLRDIIREYTDVYPEIIERACFVLEKKFGIQLKEIDKEEHLYILISTPESLAGILGTTKDTPKL
  GLLLVILGVIFMNGNRASEAVLWEALRKMGLRPGVRHPLLGDLRKLLTYEFVKQKYLDYRRVPNSNPPEY
  EFLWGLRSYHETSKMKVLRFIAEVQKRDPRDWTAQFMEAADEALDALDAAAAEAEARAEARTRMGIGDEA
  VSGPWSWDDIEFELLTWDEEGDFGDPWSRIPFTFWARYHQNARSRFPQTFAGPIIGPGGTASANFAANFG
  AIGFFWVE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006986
• ORF Size: 2334 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006986.2, NM_006986.3, NP_008917.3
• RefSeq Size: 2760 bp
• RefSeq ORF: 2337 bp
• Locus ID: 9500
• Domains: MAGE
• Protein Families: Druggable Genome
• Protein Pathways: Neurotrophin signaling pathway
• MW: 86 kDa
• Gene Summary: This gene is a member of the melanoma antigen gene (MAGE) family. Most of the genes of this family encode tumor specific antigens that are not expressed in normal adult tissues except testis. Although the protein encoded by this gene shares strong homology with members of the MAGE family, it is expressed in almost all normal adult tissues. This gene has been demonstrated to be involved in the p75 neurotrophin receptor mediated programmed cell death pathway. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]