NCOA62 (SNW1) (NM_012245) Human Tagged ORF Clone

CAT#: RC215046

SNW1 (Myc-DDK-tagged)-Human SNW domain containing 1 (SNW1)

Product Images

Western blot validation of overexpression lysate (Cat# LY402178) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215046 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SNW1
• Synonyms: Bx42; FUN20; NCOA-62; Prp45; PRPF45; SKIIP; SKIP; SKIP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215046 representing NM_012245
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCTCACCAGCTTTTTACCTGCACCTACTCAGCTATCTCAGGACCAGCTTGAGGCTGAAGAAAAGG
  CAAGATCCCAGAGATCACGGCAGACCTCACTGGTCTCCTCCCGAAGAGAACCTCCCCCGTACGGATACCG
  GAAAGGCTGGATACCTCGGTTATTAGAGGATTTTGGAGATGGAGGTGCTTTTCCAGAGATCCATGTGGCC
  CAGTATCCACTGGATATGGGACGAAAGAAAAAAATGTCGAATGCGCTGGCCATTCAGGTGGATTCTGAAG
  GAAAAATTAAATATGATGCAATTGCTCGACAAGGACAGTCAAAAGACAAGGTCATTTATAGCAAATACAC
  TGACCTGGTTCCAAAGGAGGTTATGAATGCAGATGATCCAGACCTGCAAAGGCCCGATGAAGAAGCTATT
  AAAGAGATAACAGAAAAGACAAGAGTAGCCTTAGAAAAATCTGTATCACAGAAGGTCGCCGCAGCCATGC
  CAGTTCGAGCAGCTGACAAATTGGCTCCTGCTCAGTATATCCGATACACACCATCTCAGCAAGGAGTGGC
  ATTCAACTCTGGAGCTAAACAGAGGGTTATTCGGATGGTAGAAATGCAGAAAGATCCAATGGAGCCTCCA
  AGGTTCAAGATTAATAAGAAAATTCCCCGGGGACCACCTTCTCCTCCTGCGCCTGTCATGCATTCTCCTA
  GCCGAAAGATGACTGTAAAGGAACAACAAGAGTGGAAGATTCCTCCTTGTATTTCTAACTGGAAAAATGC
  AAAGGGTTATACAATTCCATTAGACAAACGTCTGGCTGCTGATGGAAGAGGACTACAGACAGTACACATA
  AATGAAAATTTCGCCAAATTGGCAGAAGCCCTCTACATTGCTGATCGGAAGGCTCGTGAAGCTGTGGAAA
  TGCGTGCCCAAGTAGAGAGAAAAATGGCTCAGAAAGAAAAGGAAAAACATGAAGAGAAACTTAGAGAAAT
  GGCCCAGAAAGCCAGGGAAAGAAGAGCTGGGATCAAAACTCATGTGGAAAAAGAGGATGGGGAGGCACGT
  GAGAGGGATGAAATCCGGCATGACAGGCGAAAAGAGAGACAGCATGACCGGAATCTTTCCAGGGCAGCTC
  CTGATAAGAGGTCGAAACTTCAGAGAAATGAAAATCGGGATATCAGTGAAGTTATTGCTCTCGGTGTTCC
  TAATCCTCGGACTTCCAATGAAGTTCAGTATGACCAAAGGCTCTTCAACCAATCCAAGGGTATGGACAGT
  GGATTTGCAGGTGGAGAAGATGAAATTTATAATGTTTATGATCAAGCCTGGAGAGGTGGTAAAGATATGG
  CCCAGAGTATTTATAGGCCCAGTAAAAATCTGGACAAGGACATGTATGGTGATGACCTAGAAGCCAGAAT
  AAAGACCAACAGATTTGTTCCCGACAAGGAGTTTTCTGGTTCAGACCGTAGACAGAGAGGCCGAGAAGGA
  CCAGTTCAGTTTGAGGAAGATCCTTTTGGTTTGGACAAGTTTTTGGAAGAAGCCAAACAGCATGGTGGCT
  CTAAAAGACCCTCAGATAGCAGCCGCCCCAAGGAACACGAGCATGAAGGCAAGAAGAGGAGGAAGGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215046 representing NM_012245
  Red=Cloning site Green=Tags(s)
  
  MALTSFLPAPTQLSQDQLEAEEKARSQRSRQTSLVSSRREPPPYGYRKGWIPRLLEDFGDGGAFPEIHVA
  QYPLDMGRKKKMSNALAIQVDSEGKIKYDAIARQGQSKDKVIYSKYTDLVPKEVMNADDPDLQRPDEEAI
  KEITEKTRVALEKSVSQKVAAAMPVRAADKLAPAQYIRYTPSQQGVAFNSGAKQRVIRMVEMQKDPMEPP
  RFKINKKIPRGPPSPPAPVMHSPSRKMTVKEQQEWKIPPCISNWKNAKGYTIPLDKRLAADGRGLQTVHI
  NENFAKLAEALYIADRKAREAVEMRAQVERKMAQKEKEKHEEKLREMAQKARERRAGIKTHVEKEDGEAR
  ERDEIRHDRRKERQHDRNLSRAAPDKRSKLQRNENRDISEVIALGVPNPRTSNEVQYDQRLFNQSKGMDS
  GFAGGEDEIYNVYDQAWRGGKDMAQSIYRPSKNLDKDMYGDDLEARIKTNRFVPDKEFSGSDRRQRGREG
  PVQFEEDPFGLDKFLEEAKQHGGSKRPSDSSRPKEHEHEGKKRRKE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012245
• ORF Size: 1608 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012245.1, NM_012245.2, NP_036377.1
• RefSeq Size: 2146 bp
• RefSeq ORF: 1611 bp
• Locus ID: 22938
• Domains: SKIP_SNW
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Notch signaling pathway, Spliceosome
• MW: 61.3 kDa
• Gene Summary: This gene, a member of the SNW gene family, encodes a coactivator that enhances transcription from some Pol II promoters. This coactivator can bind to the ligand-binding domain of the vitamin D receptor and to retinoid receptors to enhance vitamin D-, retinoic acid-, estrogen-, and glucocorticoid-mediated gene expression. It can also function as a splicing factor by interacting with poly(A)-binding protein 2 to directly control the expression of muscle-specific genes at the transcriptional level. Finally, the protein may be involved in oncogenesis since it interacts with a region of SKI oncoproteins that is required for transforming activity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]