GRK3 (NM_005160) Human Tagged ORF Clone

CAT#: RC215129

ADRBK2 (Myc-DDK-tagged)-Human adrenergic, beta, receptor kinase 2 (ADRBK2)

Product Images

Western blot validation of overexpression lysate (Cat# LY417480) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215129 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GRK3
• Synonyms: ADRBK2; BARK2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215129 representing NM_005160
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGACCTGGAGGCTGTGCTGGCCGATGTCAGTTACCTGATGGCCATGGAGAAGAGCAAGGCGACCC
  CGGCCGCCCGCGCCAGCAAGAGGATCGTCCTGCCGGAGCCCAGTATCCGGAGTGTGATGCAGAAGTACCT
  TGCAGAGAGAAATGAAATAACCTTTGACAAGATTTTCAATCAGAAAATTGGTTTCTTGCTATTTAAAGAT
  TTTTGTTTGAATGAAATTAATGAAGCTGTACCTCAGGTGAAGTTTTATGAAGAGATAAAGGAATATGAAA
  AACTTGATAATGAGGAAGACCGCCTTTGCAGAAGTCGACAAATTTATGATGCCTACATCATGAAGGAACT
  TCTTTCCTGTTCACATCCTTTCTCAAAGCAAGCTGTAGAACACGTACAAAGTCATTTATCCAAGAAACAA
  GTGACATCAACTCTTTTTCAGCCATACATAGAAGAAATTTGTGAAAGCCTTCGAGGTGACATTTTTCAAA
  AATTTATGGAAAGTGACAAGTTCACTAGATTTTGTCAGTGGAAAAACGTTGAATTAAATATCCATTTGAC
  CATGAATGAGTTCAGTGTGCATAGGATTATTGGACGAGGAGGATTCGGGGAAGTTTATGGTTGCAGGAAA
  GCAGACACTGGAAAAATGTATGCAATGAAATGCTTAGATAAGAAGAGGATCAAAATGAAACAAGGAGAAA
  CATTAGCCTTAAATGAAAGAATCATGTTGTCTCTTGTCAGCACAGGAGACTGTCCTTTCATTGTATGTAT
  GACCTATGCCTTCCATACCCCAGATAAACTCTGCTTCATCCTGGATCTGATGAACGGGGGCGATTTGCAC
  TACCACCTTTCACAACACGGTGTGTTCTCTGAGAAGGAGATGCGGTTTTATGCCACTGAAATCATTCTGG
  GTCTGGAACACATGCACAATCGGTTTGTTGTCTACAGAGATTTGAAGCCAGCAAATATTCTCTTGGATGA
  ACATGGACACGCAAGAATATCAGATCTTGGTCTTGCCTGCGATTTTTCCAAAAAGAAGCCTCATGCGAGT
  GTTGGCACCCATGGGTACATGGCTCCCGAGGTGCTGCAGAAGGGGACGGCCTATGACAGCAGTGCCGACT
  GGTTCTCCCTGGGCTGCATGCTTTTCAAACTTCTGAGAGGTCACAGCCCTTTCAGACAACATAAAACCAA
  AGACAAGCATGAAATTGACCGAATGACACTCACCGTGAATGTGGAACTTCCAGACACCTTCTCTCCTGAA
  CTGAAGTCCCTTTTGGAGGGCTTGCTTCAGCGAGACGTTAGCAAGCGGCTGGGCTGTCACGGAGGCGGCT
  CACAGGAAGTAAAAGAGCACAGCTTTTTCAAAGGTGTTGACTGGCAGCATGTCTACTTACAAAAGTACCC
  ACCACCCTTGATTCCTCCCCGGGGAGAAGTCAATGCTGCTGATGCCTTTGATATTGGCTCATTTGATGAA
  GAGGATACCAAAGGGATTAAGCTACTTGATTGCGACCAAGAACTCTACAAGAACTTCCCTTTGGTCATCT
  CTGAACGCTGGCAGCAAGAAGTAACGGAAACAGTTTATGAAGCAGTAAATGCAGACACAGATAAAATCGA
  GGCCAGGAAGAGAGCTAAAAATAAGCAACTTGGCCACGAAGAAGATTACGCTCTGGGGAAGGACTGTATT
  ATGCACGGGTACATGCTGAAACTGGGAAACCCATTTCTGACTCAGTGGCAGCGTCGCTATTTTTACCTCT
  TTCCAAATAGACTTGAATGGAGAGGAGAGGGAGAGTCCCGGCAAAATTTACTGACAATGGAACAGATTCT
  CTCTGTGGAAGAAACTCAAATTAAAGACAAAAAATGCATTTTGTTCAGAATAAAAGGAGGGAAACAATTT
  GTCTTGCAATGTGAGAGTGATCCAGAGTTTGTGCAGTGGAAGAAAGAGTTGAACGAAACCTTCAAGGAGG
  CCCAGCGGCTATTGCGTCGTGCCCCGAAGTTCCTCAACAAACCTCGGTCAGGTACTGTGGAGCTCCCAAA
  GCCATCCCTCTGTCACAGAAACAGCAACGGCCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215129 representing NM_005160
  Red=Cloning site Green=Tags(s)
  
  MADLEAVLADVSYLMAMEKSKATPAARASKRIVLPEPSIRSVMQKYLAERNEITFDKIFNQKIGFLLFKD
  FCLNEINEAVPQVKFYEEIKEYEKLDNEEDRLCRSRQIYDAYIMKELLSCSHPFSKQAVEHVQSHLSKKQ
  VTSTLFQPYIEEICESLRGDIFQKFMESDKFTRFCQWKNVELNIHLTMNEFSVHRIIGRGGFGEVYGCRK
  ADTGKMYAMKCLDKKRIKMKQGETLALNERIMLSLVSTGDCPFIVCMTYAFHTPDKLCFILDLMNGGDLH
  YHLSQHGVFSEKEMRFYATEIILGLEHMHNRFVVYRDLKPANILLDEHGHARISDLGLACDFSKKKPHAS
  VGTHGYMAPEVLQKGTAYDSSADWFSLGCMLFKLLRGHSPFRQHKTKDKHEIDRMTLTVNVELPDTFSPE
  LKSLLEGLLQRDVSKRLGCHGGGSQEVKEHSFFKGVDWQHVYLQKYPPPLIPPRGEVNAADAFDIGSFDE
  EDTKGIKLLDCDQELYKNFPLVISERWQQEVTETVYEAVNADTDKIEARKRAKNKQLGHEEDYALGKDCI
  MHGYMLKLGNPFLTQWQRRYFYLFPNRLEWRGEGESRQNLLTMEQILSVEETQIKDKKCILFRIKGGKQF
  VLQCESDPEFVQWKKELNETFKEAQRLLRRAPKFLNKPRSGTVELPKPSLCHRNSNGL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005160
• ORF Size: 2064 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005160.1, NM_005160.2, NM_005160.3, NP_005151.1
• RefSeq Size: 3628 bp
• RefSeq ORF: 2067 bp
• Locus ID: 157
• Cytogenetics: 22q12.1
• Domains: RGS, pkinase, S_TK_X, TyrKc, PH, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway, Endocytosis, Olfactory transduction
• MW: 79.5 kDa
• Gene Summary: 'The beta-adrenergic receptor kinase specifically phosphorylates the agonist-occupied form of the beta-adrenergic and related G protein-coupled receptors. Overall, the beta adrenergic receptor kinase 2 has 85% amino acid similarity with beta adrenergic receptor kinase 1, with the protein kinase catalytic domain having 95% similarity. These data suggest the existence of a family of receptor kinases which may serve broadly to regulate receptor function. [provided by RefSeq, Jul 2008]'