GRK3 (NM_005160) Human Untagged Clone

CAT#: SC116917

ADRBK2 (untagged)-ORIGENE UNIQUE VARIANT 1 of Human adrenergic, beta, receptor kinase 2 (ADRBK2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GRK3
• Synonyms: ADRBK2; BARK2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005160, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGACCTGGAGGCTGTGCTGGCCGATGTCAGTTACCTGATGGCCATGGAGAAGAGCAAGGCGACCC
  CGGCCGCCCGCGCCAGCAAGAGGATCGTCCTGCCGGAGCCCAGTATCCGGAGTGTGATGCAGAAGTACCT
  TGCAGAGAGAAATGAAATAACCTTTGACAAGATTTTCAATCAGAAAATTGGTTTCTTGCTATTTAAAGAT
  TTTTGTTTGAATGAAATTAATGAAGCTGTACCTCAGGTGAAGTTTTATGAAGAGATAAAGGAATATGAAA
  AACTTGATAATGAGGAAGACCGCCTTTGCAGAAGTCGACAAATTTATGATGCCTACATCATGAAGGAACT
  TCTTTCCTGTTCACATCCTTTCTCAAAGCAAGCTGTAGAACACGTACAAAGTCATTTATCCAAGAAACAA
  GTGACATCAACTCTTTTTCAGCCATACATAGAAGAAATTTGTGAAAGCCTTCGAGGTGACATTTTTCAAA
  AATTTATGGAAAGTGACAAGTTCACTAGATTTTGTCAGTGGAAAAACGTTGAATTAAATATCCATTTGAC
  CATGAATGAGTTCAGTGTGCATAGGATTATTGGACGAGGAGGATTCGGGGAAGTTTATGGTTGCAGGAAA
  GCAGACACTGGAAAAATGTATGCAATGAAATGCTTAGATAAGAAGAGGATCAAAATGAAACAAGGAGAAA
  CATTAGCCTTAAATGAAAGAATCATGTTGTCTCTTGTCAGCACAGGAGACTGTCCTTTCATTGTATGTAT
  GACCTATGCCTTCCATACCCCAGATAAACTCTGCTTCATCCTGGATCTGATGAACGGGGGCGATTTGCAC
  TACCACCTTTCACAACACGGTGTGTTCTCTGAGAAGGAGATGCGGTTTTATGCCACTGAAATCATTCTGG
  GTCTGGAACACATGCACAATCGGTTTGTTGTCTACAGAGATTTGAAGCCAGCAAATATTCTCTTGGATGA
  ACATGGACACGCAAGAATATCAGATCTTGGTCTTGCCTGCGATTTTTCCAAAAAGAAGCCTCATGCGAGT
  GTTGGCACCCATGGGTACATGGCTCCCGAGGTGCTGCAGAAGGGGACGGCCTATGACAGCAGTGCCGACT
  GGTTCTCCCTGGGCTGCATGCTTTTCAAACTTCTGAGAGGTCACAGCCCTTTCAGACAACATAAAACCAA
  AGACAAGCATGAAATTGACCGAATGACACTCACCGTGAATGTGGAACTTCCAGACACCTTCTCTCCTGAA
  CTGAAGTCCCTTTTGGAGGGCTTGCTTCAGCGAGACGTTAGCAAGCGGCTGGGCTGTCACGGAGGCGGCT
  CACAGGAAGTAAAAGAGCACAGCTTTTTCAAAGGTGTTGACTGGCAGCATGTCTACTTACAAAAGTACCC
  ACCACCCTTGATTCCTCCCCGGGGAGAAGTCAATGCTGCTGATGCCTTTGATATTGGCTCATTTGATGAA
  GAGGATACCAAAGGGATTAAGCTACTTGATTGCGACCAAGAACTCTACAAGAACTTCCCTTTGGTCATCT
  CTGAACGCTGGCAGCAAGAAGTAACGGAAACAGTTTATGAAGCAGTAAATGCAGACACAGATAAAATCGA
  GGCCAGGAAGAGAGCTAAAAATAAGCAACTTGGCCACGAAGAAGATTACGCTCTGGGGAAGGACTGTATT
  ATGCACGGGTACATGCTGAAACTGGGAAACCCATTTCTGACTCAGTGGCAGCGTCGCTATTTTTACCTCT
  TTCCAAATAGACTTGAATGGAGAGGAGAGGGAGAGTCCCGGCAAAATTTACTGACAATGGAACAGATTCT
  CTCTGTGGAAGAAACTCAAATTAAAGACAAAAAATGCATTTTGTTCAGAATAAAAGGAGGGAAACAATTT
  GTCTTGCAATGTGAGAGTGATCCAGAGTTTGTGCAGTGGAAGAAAGAGTTGAACGAAACCTTCAAGGAGG
  CCCAGCGGCTATTGCGTCGTGCCCCGAAGTTCCTCAACAAACCTCGGTCAGGTACTGTGGAGCTCCCAAA
  GCCATCCCTCTGTCACAGAAACAGCAACGGCCTCTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005160 unedited
  TGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGTGGAGGCTGTGCTGGCCGA
  TGTCAGTTACCTGGATGGCCATGGAGAAGAGCAAGGCGACCCCGGCCGCCCGCGCCAGCA
  AGAGGATCGTCCTGCCGGAGCCCAGTATCCGGAGTGTGATGCAGAAGTACCTTGCAGAGA
  GAAATGAAATAACCTTTGACAAGATTTTCAATCAGAAAATTGATAAAGGAATATGAAAAA
  CTTGATAATGAGGAAGACCGCCTTTGCAGAAGTCGACAAATTTATGATGCCTACATCATG
  AAGGAACTTCTTTCCTGTTCACATCCTTTCTCAAAGCAAGCTGTAGAACACGTACAAAGT
  CATTTATCCAAGAAACAAGTGACATCAACTCTTTTTCAGCCATACATAGAAGAAATTTGT
  GAAAGCCTTCGAGGTGACATTTTTCAAAAATTTATGGAAAGTGACAAGTTCACTAGATTT
  TGTCAGTGGAAAAACGTTGAATTAAATATCCATTTGACCATGAATGAGTTCAGTGTGCAT
  AGGATTATTGGACGAGGAGGATTCGGGGAAGTTTATGGTTGCAGGAAAGCAGACACTGGA
  AAAATGTATGCAATGAAATGCTTAGATAAGAAGAGGATCAAAATGAAACAAGGAGAAACA
  TTAGCCTTAAATGAAAGAATCATGTTGTCTCTTGTCAGCACAGAGACTGTCCTTTCATTG
  TATGTATGACCTATGCCTTCCATACCCCAGATAAACTCTGCTTCATCCTGGATCTGATGA
  ACGGNGGCGATTTGCACTACCACCTTTCACAACACGGTGTGTTCTCTGAGAAGAGNATGG
  CGNTNTATGCCCTGAAATATTCTGGGTCTGGACACATGCCATTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005160
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone was found to represent an alternative form of the specific reference to which it is associated. Its Open Reading Frame (ORF) may represent a novel form or alternative splice variant. By virtue of it being a true transcript (cDNA clone not PCR product), it provides a biologically relevant copy of its mRNA template. For more details, please evaluate the sequence information provided on this website or contact our customer care specialists.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005160.2, NP_005151.1
• RefSeq Size: 3628 bp
• RefSeq ORF: 2067 bp
• Locus ID: 157
• Cytogenetics: 22q12.1
• Domains: RGS, pkinase, S_TK_X, TyrKc, PH, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway, Endocytosis, Olfactory transduction
• Gene Summary: 'The beta-adrenergic receptor kinase specifically phosphorylates the agonist-occupied form of the beta-adrenergic and related G protein-coupled receptors. Overall, the beta adrenergic receptor kinase 2 has 85% amino acid similarity with beta adrenergic receptor kinase 1, with the protein kinase catalytic domain having 95% similarity. These data suggest the existence of a family of receptor kinases which may serve broadly to regulate receptor function. [provided by RefSeq, Jul 2008]'