GRK3 (NM_005160) Human Untagged Clone

CAT#: SC323529

ADRBK2 (untagged)-Kinase deficient mutant (K220M) of Human adrenergic, beta, receptor kinase 2 (ADRBK2)


  "NM_005160" in other vectors (8)

Reconstitution Protocol

USD 1,400.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol GRK3
Synonyms ADRBK2; BARK2
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC323529 sequence for NM_005160 edited (data generated by NextGen Sequencing)
ATGGCGGACCTGGAGGCTGTGCTGGCCGATGTCAGTTACCTGATGGCCATGGAGAAGAGC
AAGGCGACCCCGGCCGCCCGCGCCAGCAAGAGGATCGTCCTGCCGGAGCCCAGTATCCGG
AGTGTGATGCAGAAGTACCTTGCAGAGAGAAATGAAATAACCTTTGACAAGATTTTCAAT
CAGAAAATTGGTTTCTTGCTATTTAAAGATTTTTGTTTGAATGAAATTAATGAAGCTGTA
CCTCAGGTGAAGTTTTATGAAGAGATAAAGGAATATGAAAAACTTGATAATGAGGAAGAC
CGCCTTTGCAGAAGTCGACAAATTTATGATGCCTACATCATGAAGGAACTTCTTTCCTGT
TCACATCCTTTCTCAAAGCAAGCTGTAGAACACGTACAAAGTCATTTATCCAAGAAACAA
GTGACATCAACTCTTTTTCAGCCATACATAGAAGAAATTTGTGAAAGCCTTCGAGGTGAC
ATTTTTCAAAAATTTATGGAAAGTGACAAGTTCACTAGATTTTGTCAGTGGAAAAACGTT
GAATTAAATATCCATTTGACCATGAATGAGTTCAGTGTGCATAGGATTATTGGACGAGGA
GGATTCGGGGAAGTTTATGGTTGCAGGAAAGCAGACACTGGAAAAATGTATGCAATGATG
TGCTTAGATAAGAAGAGGATCAAAATGAAACAAGGAGAAACATTAGCCTTAAATGAAAGA
ATCATGTTGTCTCTTGTCAGCACAGGAGACTGTCCTTTCATTGTATGTATGACCTATGCC
TTCCATACCCCAGATAAACTCTGCTTCATCCTGGATCTGATGAACGGGGGCGATTTGCAC
TACCACCTTTCACAACACGGTGTGTTCTCTGAGAAGGAGATGCGGTTTTATGCCACTGAA
ATCATTCTGGGTCTGGAACACATGCACAATCGGTTTGTTGTCTACAGAGATTTGAAGCCA
GCAAATATTCTCTTGGATGAACATGGACACGCAAGAATATCAGATCTTGGTCTTGCCTGC
GATTTTTCCAAAAAGAAGCCTCATGCGAGTGTTGGCACCCATGGGTACATGGCTCCCGAG
GTGCTGCAGAAGGGGACGGCCTATGACAGCAGTGCCGACTGGTTCTCCCTGGGCTGCATG
CTTTTCAAACTTCTGAGAGGTCACAGCCCTTTCAGACAACATAAAACCAAAGACAAGCAT
GAAATTGACCGAATGACACTCACCGTGAATGTGGAACTTCCAGACACCTTCTCTCCTGAA
CTGAAGTCCCTTTTGGAGGGCTTGCTTCAGCGAGACGTTAGCAAGCGGCTGGGCTGTCAC
GGAGGCGGCTCACAGGAAGTAAAAGAGCACAGCTTTTTCAAAGGTGTTGACTGGCAGCAT
GTCTACTTACAAAAGTACCCACCACCCTTGATTCCTCCCCGGGGAGAAGTCAATGCTGCT
GATGCCTTTGATATTGGCTCATTTGATGAAGAGGATACCAAAGGGATTAAGCTACTTGAT
TGCGACCAAGAACTCTACAAGAACTTCCCTTTGGTCATCTCTGAACGCTGGCAGCAAGAA
GTAACGGAAACAGTTTATGAAGCAGTAAATGCAGACACAGATAAAATCGAGGCCAGGAAG
AGAGCTAAAAATAAGCAACTTGGCCACGAAGAAGATTACGCTCTGGGGAAGGACTGTATT
ATGCACGGGTACATGCTGAAACTGGGAAACCCATTTCTGACTCAGTGGCAGCGTCGCTAT
TTTTACCTCTTTCCAAATAGACTTGAATGGAGAGGAGAGGGAGAGTCCCGGCAAAATTTA
CTGACAATGGAACAGATTCTCTCTGTGGAAGAAACTCAAATTAAAGACAAAAAATGCATT
TTGTTCAGAATAAAAGGAGGGAAACAATTTGTCTTGCAATGTGAGAGTGATCCAGAGTTT
GTGCAGTGGAAGAAAGAGTTGAACGAAACCTTCAAGGAGGCCCAGCGGCTATTGCGTCGT
GCCCCGAAGTTCCTCAACAAACCTCGGTCAGGTACTGTGGAGCTCCCAAAGCCATCCCTC
TGTCACAGAAACAGCAACGGCCTCTAG

Clone variation with respect to NM_005160.3
659 a=>t;660 a=>g
>OriGene 5' read for mutant NM_005160 unedited
CCCGCCCGTCTCAGCAAATGGGCGGTAGGCGCTGTACGGTTGGGAGGATCTATATAAGCAGAGCTCGTTT
AGTTGAACCGTCAGAATCCTGTAATACGACTCACTATAGGGCGGCCGCGAATCCGGCACGAGGCCAAAGC
TCGCCAACATGGCGGACCTGGAGGCCGTGCTGGCCGATGTCAGTAACCTGATGGCCATGGAGAAGAGCAA
GGCGACCCCGGCCGCCCGCGCCAGCAAGAGGAACGTCCTGCCGGAGCCCAGTATCCGGAGTGTGATGCAG
AAGTACCTTGCAGAGAGAAATGAAATAACCTTTGACAAGATTTTCAATCAGAAAATTGGTTTCTTGCTAT
TTAAAGATTTTTGTTTGAATGAAATTAATGAAGCTGTACCTCAGGTGAAGTTTTATGAAGAGATAAAGGA
ATATGAAAAACTTGATAATGAGGAAGACCGCCTTTGCAGAAGTCGACAAATTTATGATGCCTACATCATG
AAGGAACTTCTTTCCTGTTCACATCCTTTCTCAAAGCAAGCTGTAGAACACGTACAAAGTCATTTATCCA
AGAACAAGTGACATCAACTCTTTTTCAGCCATACATAGAAGAATTTGTGAAAGCCTTCGAGGTGACATTT
TTCAAAAATTTATGGAAAGTGACAAGTTCACTAGATTTTGTCAGTGGAAAAACGTTGATTAAATATCCAT
TTGACCATGAATGAGTTCAGTGTGCATAGATTATTGGACGAGAGATCGGGGAAGTTATGGTTGCAGAAGC
AAACCCTGGAAAATGTTATGCATGATGTGCTAGTAGAGAGGATCGATGAACAGGAAAACATAGCTATGAA
GATCATGTTGTCTCTGTCAGCCAGGAACTGTCTTCATGTAATGTATGACTATGCTTCTAACCGATTACTT
GCTTCATCTGGATTCGATGACCGGGGCGATGCTACACCTCAACGGTGTTTCTCTGCAGAGAGATGCGGTT
ATGCTG
>SC323529 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TYGTGTGCAWAGGWTATTGGACGAGGAGGATTCGGGGAAGTTTATGGTTGCAGGAAAGCAGACACTGGAA
AAATGTATGCAATGATGTGCTTAGATAAGAAGAGGATCAAAATGAAACAAGGAGAAACATTAGCCTTAAA
TGAAAGAATCATGTTGTCTCTTGTCAGCACAGGAGACTGTCCTTTCATTGTATGTATGACCTATGCCTTC
CATACCCCAGATAAACTCTGCTTCATCCTGGATCTGATGAACGGG
Restriction Sites Please inquire     
ACCN NM_005160
Insert Size 3370 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005160.2, NP_005151.1
RefSeq Size 3628 bp
RefSeq ORF 2067 bp
Locus ID 157
Cytogenetics 22q12.1
Domains RGS, pkinase, S_TK_X, TyrKc, PH, S_TKc
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Chemokine signaling pathway, Endocytosis, Olfactory transduction
Gene Summary 'The beta-adrenergic receptor kinase specifically phosphorylates the agonist-occupied form of the beta-adrenergic and related G protein-coupled receptors. Overall, the beta adrenergic receptor kinase 2 has 85% amino acid similarity with beta adrenergic receptor kinase 1, with the protein kinase catalytic domain having 95% similarity. These data suggest the existence of a family of receptor kinases which may serve broadly to regulate receptor function. [provided by RefSeq, Jul 2008]'

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