FTSJ1 (NM_012280) Human Tagged ORF Clone

CAT#: RC215210

FTSJ1 (Myc-DDK-tagged)-Human FtsJ homolog 1 (E. coli) (FTSJ1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY415848) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215210 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FTSJ1
• Synonyms: CDLIV; JM23; MRX9; MRX44; SPB1; TRMT7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215210 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGACGGACGTCAAAGGACAAGCGGGATGTCTACTACCGCCTGGCCAAGGAGAATGGCTGGCGTGCTC
  GCAGCGCCTTCAAACTGCTACAACTGGATAAGGAATTCCAACTCTTCCAAGGCGTGACACGGGCAGTTGA
  CCTGTGTGCAGCCCCAGGCAGCTGGAGCCAGGTGCTGAGCCAGAAGATCGGGGGCCAAGGGTCCGGCCAC
  GTGGTGGCTGTGGACCTGCAGGCTATGGCTCCACTACCAGGTGTGGTACAGATCCAGGGGGACATCACCC
  AGCTGTCCACTGCCAAGGAGATCATCCAGCACTTTAAGGGCTGCCCTGCGGACCTAGTGGTGTGTGACGG
  GGCTCCTGATGTAACCGGTCTCCATGATGTTGATGAGTATATGCAGGCCCAGCTCCTCCTAGCTGCTCTG
  AACATTGCTACACATGTCCTGAAGCCAGGGGGCTGCTTTGTGGCCAAGATATTCCGAGGCCGGGATGTGA
  CGCTCCTCTACAGCCAGCTGCAGGTCTTCTTCTCCAGCGTGCTGTGTGCCAAGCCCAGGAGCAGCCGGAA
  CTCTAGCATCGAGGCCTTCGCTGTCTGTCAGGGCTATGACCCTCCCGAGGGCTTCATCCCGGACCTGAGC
  AAACCCCTGCTGGACCATTCTTACGACCCAGATTTCAACCAGCTGGATGGTCCCACCCGCATCATTGTGC
  CTTTTGTGACCTGTGGGGACCTGAGCTCCTATGATTCGGACCGCAGTTACCCACTGGACCTAGAGGGCGG
  CTCAGAGTACAAGTACACTCCACCCACACAGCCCCCCATCTCGCCACCATACCAGGAGGCCTGCACGTTG
  AAGAGGAAGGGGCAGCTGGCCAAGGAGATCCGCCCCCAGGACTGCCCCATCAGCAGAGTGGACACGTTTC
  CCCAGCCCCTGGCCGCCCCTCAGTGCCACACCCTGCTGGCCCCTGAGATGGAAGACAATGAAATGAGTTG
  TTCACCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215210 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGRTSKDKRDVYYRLAKENGWRARSAFKLLQLDKEFQLFQGVTRAVDLCAAPGSWSQVLSQKIGGQGSGH
  VVAVDLQAMAPLPGVVQIQGDITQLSTAKEIIQHFKGCPADLVVCDGAPDVTGLHDVDEYMQAQLLLAAL
  NIATHVLKPGGCFVAKIFRGRDVTLLYSQLQVFFSSVLCAKPRSSRNSSIEAFAVCQGYDPPEGFIPDLS
  KPLLDHSYDPDFNQLDGPTRIIVPFVTCGDLSSYDSDRSYPLDLEGGSEYKYTPPTQPPISPPYQEACTL
  KRKGQLAKEIRPQDCPISRVDTFPQPLAAPQCHTLLAPEMEDNEMSCSP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012280
• ORF Size: 987 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012280.1, NM_012280.2, NM_012280.3, NP_036412.1
• RefSeq Size: 2091 bp
• RefSeq ORF: 990 bp
• Locus ID: 24140
• Domains: FtsJ
• MW: 36.1 kDa
• Gene Summary: This gene encodes a member of the methyltransferase superfamily. The encoded protein localizes to the nucleolus, binds to S-adenosylmethionine, and may be involved in the processing and modification of ribosomal RNA. Mutations in this gene are associated with cognitive disability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]