FTSJ1 (NM_012280) Human Tagged ORF Clone

CAT#: RG215210

FTSJ1 (GFP-tagged) - Human FtsJ homolog 1 (E. coli) (FTSJ1), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: FTSJ1
• Synonyms: CDLIV; JM23; MRX9; MRX44; SPB1; TRMT7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG215210 representing NM_012280
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGACGGACGTCAAAGGACAAGCGGGATGTCTACTACCGCCTGGCCAAGGAGAATGGCTGGCGTGCTC
  GCAGCGCCTTCAAACTGCTACAACTGGATAAGGAATTCCAACTCTTCCAAGGCGTGACACGGGCAGTTGA
  CCTGTGTGCAGCCCCAGGCAGCTGGAGCCAGGTGCTGAGCCAGAAGATCGGGGGCCAAGGGTCCGGCCAC
  GTGGTGGCTGTGGACCTGCAGGCTATGGCTCCACTACCAGGTGTGGTACAGATCCAGGGGGACATCACCC
  AGCTGTCCACTGCCAAGGAGATCATCCAGCACTTTAAGGGCTGCCCTGCGGACCTAGTGGTGTGTGACGG
  GGCTCCTGATGTAACCGGTCTCCATGATGTTGATGAGTATATGCAGGCCCAGCTCCTCCTAGCTGCTCTG
  AACATTGCTACACATGTCCTGAAGCCAGGGGGCTGCTTTGTGGCCAAGATATTCCGAGGCCGGGATGTGA
  CGCTCCTCTACAGCCAGCTGCAGGTCTTCTTCTCCAGCGTGCTGTGTGCCAAGCCCAGGAGCAGCCGGAA
  CTCTAGCATCGAGGCCTTCGCTGTCTGTCAGGGCTATGACCCTCCCGAGGGCTTCATCCCGGACCTGAGC
  AAACCCCTGCTGGACCATTCTTACGACCCAGATTTCAACCAGCTGGATGGTCCCACCCGCATCATTGTGC
  CTTTTGTGACCTGTGGGGACCTGAGCTCCTATGATTCGGACCGCAGTTACCCACTGGACCTAGAGGGCGG
  CTCAGAGTACAAGTACACTCCACCCACACAGCCCCCCATCTCGCCACCATACCAGGAGGCCTGCACGTTG
  AAGAGGAAGGGGCAGCTGGCCAAGGAGATCCGCCCCCAGGACTGCCCCATCAGCAGAGTGGACACGTTTC
  CCCAGCCCCTGGCCGCCCCTCAGTGCCACACCCTGCTGGCCCCTGAGATGGAAGACAATGAAATGAGTTG
  TTCACCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG215210 representing NM_012280
  Red=Cloning site Green=Tags(s)
  
  MGRTSKDKRDVYYRLAKENGWRARSAFKLLQLDKEFQLFQGVTRAVDLCAAPGSWSQVLSQKIGGQGSGH
  VVAVDLQAMAPLPGVVQIQGDITQLSTAKEIIQHFKGCPADLVVCDGAPDVTGLHDVDEYMQAQLLLAAL
  NIATHVLKPGGCFVAKIFRGRDVTLLYSQLQVFFSSVLCAKPRSSRNSSIEAFAVCQGYDPPEGFIPDLS
  KPLLDHSYDPDFNQLDGPTRIIVPFVTCGDLSSYDSDRSYPLDLEGGSEYKYTPPTQPPISPPYQEACTL
  KRKGQLAKEIRPQDCPISRVDTFPQPLAAPQCHTLLAPEMEDNEMSCSP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012280
• ORF Size: 987 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012280.2, NP_036412.1
• RefSeq Size: 1951
• RefSeq ORF: 990
• Locus ID: 24140
• Domains: FtsJ
• Gene Summary: This gene encodes a member of the methyltransferase superfamily. The encoded protein localizes to the nucleolus, binds to S-adenosylmethionine, and may be involved in the processing and modification of ribosomal RNA. Mutations in this gene are associated with cognitive disability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]