IWS1 (NM_017969) Human Tagged ORF Clone

CAT#: RC215212

IWS1 (Myc-DDK-tagged)-Human IWS1 homolog (S. cerevisiae) (IWS1). Note: ORF is codon optimized

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IWS1
• Synonyms: DKFZp761G0123; FLJ10006; FLJ14655; FLJ32319; MGC126375; MGC126376
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215212 ORF sequence, codon optimized.
  Due to the complexity of NM_017969, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTCCGAATATTACTCTGGAGATCAGTCTGACGACGGCGGGGCAACCCCCGTGCAGGATGAGCGGG
  ATAGTGGGTCTGATGGGGAAGATGACGTCAACGAACAGCACAGCGGTAGTGACACGGGTAGCGTAGAGAG
  GCACAGCGAAAATGAGACAAGTGACCGGGAAGACGGCTTGCCCAAGGGCCACCACGTCACAGATTCAGAG
  AATGACGAACCTCTGAACCTGAATGCGTCTGACAGCGAGTCAGAAGAGTTGCATCGACAGAAAGACAGTG
  ATTCCGAGAGCGAGGAGCGGGCGGAACCACCAGCTTCTGATTCCGAGAATGAAGATGTGAACCAGCATGG
  CAGCGACTCCGAATCTGAGGAGACTAGAAAGCTTCCAGGCTCTGACTCCGAGAACGAAGAACTGCTCAAC
  GGGCACGCTAGTGACAGCGAGAACGAAGACGTTGGCAAACATCCGGCTAGCGATAGCGAAATTGAAGAGC
  TGCAAAAATCTCCCGCCAGTGATTCCGAAACCGAGGACGCACTTAAGCCCCAAATCAGCGACAGCGAATC
  CGAGGAACCGCCCCGGCACCAGGCCTCCGACAGCGAGAATGAAGAGCCGCCTAAGCCCCGCATGTCTGAT
  TCAGAGTCCGAGGAACTTCCCAAACCTCAGGTCAGTGACTCCGAAAGCGAGGAACCTCCCCGCCACCAGG
  CTAGCGATAGTGAAAACGAAGAGCTGCCTAAGCCCCGGATATCCGACTCCGAGTCTGAGGATCCGCCAAG
  ACATCAGGCCTCAGACTCCGAGAACGAGGAATTGCCGAAACCAAGAATTTCAGACAGCGAGTCTGAGGAC
  CCTCCTCGGAATCAGGCTAGCGATTCCGAAAACGAGGAGCTCCCTAAACCGAGAGTGAGCGACAGCGAAA
  GCGAGGGCCCCCAGAAAGGCCCAGCCAGCGATTCCGAAACAGAGGATGCCTCAAGACATAAACAGAAACC
  TGAAAGTGACGATGACAGTGACAGGGAAAATAAAGGTGAGGATACGGAAATGCAGAATGATAGCTTCCAC
  TCTGATTCACACATGGATCGGAAAAAATTTCATTCCTCAGATTCCGAGGAAGAGGAACACAAGAAGCAGA
  AAATGGATAGTGACGAAGATGAGAAGGAAGGTGAAGAGGAGAAGGTGGCCAAAAGGAAAGCCGCTGTCCT
  GTCTGACAGTGAGGACGAGGAAAAGGCAAGCGCCAAGAAGAGCCGCGTAGTCAGCGACGCAGATGATAGT
  GATTCTGATGCAGTCAGTGACAAATCCGGAAAACGAGAGAAAACCATTGCAAGCGATAGTGAAGAGGAGG
  CTGGCAAAGAACTGTCCGATAAGAAGAACGAAGAGAAGGACCTTTTTGGGTCAGACTCCGAGTCAGGAAA
  CGAAGAAGAGAATTTGATCGCAGACATCTTCGGGGAATCAGGCGATGAGGAGGAGGAAGAGTTCACTGGG
  TTCAACCAGGAGGATTTGGAAGAAGAGAAGGGAGAGACACAAGTGAAAGAGGCCGAAGATTCTGATTCCG
  ACGATAACATTAAGCGCGGGAAACATATGGATTTCCTGTCAGACTTTGAAATGATGTTGCAGAGGAAAAA
  AAGTATGTCCGGAAAGCGAAGACGCAATCGGGACGGAGGGACCTTTATCTCCGACGCTGACGATGTGGTG
  AGTGCAATGATAGTCAAGATGAATGAAGCGGCTGAAGAAGACCGCCAGCTCAACAATCAGAAGAAACCAG
  CTCTCAAGAAGCTGACTCTTCTTCCCGCTGTGGTGATGCACCTCAAGAAGCAAGATCTGAAAGAAACTTT
  CATCGACAGCGGAGTAATGAGCGCTATAAAGGAATGGTTGAGTCCCCTCCCGGACAGGAGTCTTCCGGCA
  CTGAAAATTCGGGAAGAGTTGTTGAAGATTCTGCAGGAGCTGCCATCCGTATCACAGGAAACCCTGAAAC
  ACAGCGGCATAGGCCGAGCCGTGATGTACCTGTATAAGCACCCAAAGGAATCTCGCTCTAATAAGGACAT
  GGCCGGCAAACTGATCAATGAGTGGAGTAGACCCATTTTTGGTTTGACCTCCAATTACAAGGGGATGACA
  AGAGAGGAGCGGGAACAACGCGACCTGGAACAGATGCCCCAGAGACGGCGGATGAATAGTACCGGTGGGC
  AGACACCACGCAGAGATCTTGAGAAAGTGCTGACCGGAGAGGAGAAAGCCCTCAGACCGGGTGATCCGGG
  ATTCTGTGCCAGGGCCCGGGTGCCCATGCCCAGCAATAAGGATTACGTGGTCCGCCCTAAATGGAACGTT
  GAGATGGAGAGCAGTCGCTTCCAAGCCACTAGTAAGAAGGGTATTTCACGGCTGGACAAACAGATGCGCA
  AATTTACCGACATCAGAAAGAAGTCTCGGTCTGCTCATGCTGTTAAGATTTCCATCGAGGGCAATAAGAT
  GCCACTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215212 representing NM_017969
  Red=Cloning site Green=Tags(s)
  
  MDSEYYSGDQSDDGGATPVQDERDSGSDGEDDVNEQHSGSDTGSVERHSENETSDREDGLPKGHHVTDSE
  NDEPLNLNASDSESEELHRQKDSDSESEERAEPPASDSENEDVNQHGSDSESEETRKLPGSDSENEELLN
  GHASDSENEDVGKHPASDSEIEELQKSPASDSETEDALKPQISDSESEEPPRHQASDSENEEPPKPRMSD
  SESEELPKPQVSDSESEEPPRHQASDSENEELPKPRISDSESEDPPRHQASDSENEELPKPRISDSESED
  PPRNQASDSENEELPKPRVSDSESEGPQKGPASDSETEDASRHKQKPESDDDSDRENKGEDTEMQNDSFH
  SDSHMDRKKFHSSDSEEEEHKKQKMDSDEDEKEGEEEKVAKRKAAVLSDSEDEEKASAKKSRVVSDADDS
  DSDAVSDKSGKREKTIASDSEEEAGKELSDKKNEEKDLFGSDSESGNEEENLIADIFGESGDEEEEEFTG
  FNQEDLEEEKGETQVKEAEDSDSDDNIKRGKHMDFLSDFEMMLQRKKSMSGKRRRNRDGGTFISDADDVV
  SAMIVKMNEAAEEDRQLNNQKKPALKKLTLLPAVVMHLKKQDLKETFIDSGVMSAIKEWLSPLPDRSLPA
  LKIREELLKILQELPSVSQETLKHSGIGRAVMYLYKHPKESRSNKDMAGKLINEWSRPIFGLTSNYKGMT
  REEREQRDLEQMPQRRRMNSTGGQTPRRDLEKVLTGEEKALRPGDPGFCARARVPMPSNKDYVVRPKWNV
  EMESSRFQATSKKGISRLDKQMRKFTDIRKKSRSAHAVKISIEGNKMPL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017969
• ORF Size: 2457 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_017969.1, NM_017969.2, NP_060439.1
• RefSeq Size: 2973
• RefSeq ORF: 2460
• Locus ID: 55677
• MW: 92 kDa