IWS1 (NM_017969) Human Tagged ORF Clone
CAT#: RG215212
- TrueORF®
IWS1 (GFP-tagged) - Human IWS1 homolog (S. cerevisiae) (IWS1). Note: ORF is codon optimized
"NM_017969" in other vectors (2)
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Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | IWS1 |
Synonyms | DKFZp761G0123; FLJ10006; FLJ14655; FLJ32319; MGC126375; MGC126376 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG215212 ORF sequence, codon optimized.
Due to the complexity of NM_017969, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTCCGAATATTACTCTGGAGATCAGTCTGACGACGGCGGGGCAACCCCCGTGCAGGATGAGCGGG ATAGTGGGTCTGATGGGGAAGATGACGTCAACGAACAGCACAGCGGTAGTGACACGGGTAGCGTAGAGAG GCACAGCGAAAATGAGACAAGTGACCGGGAAGACGGCTTGCCCAAGGGCCACCACGTCACAGATTCAGAG AATGACGAACCTCTGAACCTGAATGCGTCTGACAGCGAGTCAGAAGAGTTGCATCGACAGAAAGACAGTG ATTCCGAGAGCGAGGAGCGGGCGGAACCACCAGCTTCTGATTCCGAGAATGAAGATGTGAACCAGCATGG CAGCGACTCCGAATCTGAGGAGACTAGAAAGCTTCCAGGCTCTGACTCCGAGAACGAAGAACTGCTCAAC GGGCACGCTAGTGACAGCGAGAACGAAGACGTTGGCAAACATCCGGCTAGCGATAGCGAAATTGAAGAGC TGCAAAAATCTCCCGCCAGTGATTCCGAAACCGAGGACGCACTTAAGCCCCAAATCAGCGACAGCGAATC CGAGGAACCGCCCCGGCACCAGGCCTCCGACAGCGAGAATGAAGAGCCGCCTAAGCCCCGCATGTCTGAT TCAGAGTCCGAGGAACTTCCCAAACCTCAGGTCAGTGACTCCGAAAGCGAGGAACCTCCCCGCCACCAGG CTAGCGATAGTGAAAACGAAGAGCTGCCTAAGCCCCGGATATCCGACTCCGAGTCTGAGGATCCGCCAAG ACATCAGGCCTCAGACTCCGAGAACGAGGAATTGCCGAAACCAAGAATTTCAGACAGCGAGTCTGAGGAC CCTCCTCGGAATCAGGCTAGCGATTCCGAAAACGAGGAGCTCCCTAAACCGAGAGTGAGCGACAGCGAAA GCGAGGGCCCCCAGAAAGGCCCAGCCAGCGATTCCGAAACAGAGGATGCCTCAAGACATAAACAGAAACC TGAAAGTGACGATGACAGTGACAGGGAAAATAAAGGTGAGGATACGGAAATGCAGAATGATAGCTTCCAC TCTGATTCACACATGGATCGGAAAAAATTTCATTCCTCAGATTCCGAGGAAGAGGAACACAAGAAGCAGA AAATGGATAGTGACGAAGATGAGAAGGAAGGTGAAGAGGAGAAGGTGGCCAAAAGGAAAGCCGCTGTCCT GTCTGACAGTGAGGACGAGGAAAAGGCAAGCGCCAAGAAGAGCCGCGTAGTCAGCGACGCAGATGATAGT GATTCTGATGCAGTCAGTGACAAATCCGGAAAACGAGAGAAAACCATTGCAAGCGATAGTGAAGAGGAGG CTGGCAAAGAACTGTCCGATAAGAAGAACGAAGAGAAGGACCTTTTTGGGTCAGACTCCGAGTCAGGAAA CGAAGAAGAGAATTTGATCGCAGACATCTTCGGGGAATCAGGCGATGAGGAGGAGGAAGAGTTCACTGGG TTCAACCAGGAGGATTTGGAAGAAGAGAAGGGAGAGACACAAGTGAAAGAGGCCGAAGATTCTGATTCCG ACGATAACATTAAGCGCGGGAAACATATGGATTTCCTGTCAGACTTTGAAATGATGTTGCAGAGGAAAAA AAGTATGTCCGGAAAGCGAAGACGCAATCGGGACGGAGGGACCTTTATCTCCGACGCTGACGATGTGGTG AGTGCAATGATAGTCAAGATGAATGAAGCGGCTGAAGAAGACCGCCAGCTCAACAATCAGAAGAAACCAG CTCTCAAGAAGCTGACTCTTCTTCCCGCTGTGGTGATGCACCTCAAGAAGCAAGATCTGAAAGAAACTTT CATCGACAGCGGAGTAATGAGCGCTATAAAGGAATGGTTGAGTCCCCTCCCGGACAGGAGTCTTCCGGCA CTGAAAATTCGGGAAGAGTTGTTGAAGATTCTGCAGGAGCTGCCATCCGTATCACAGGAAACCCTGAAAC ACAGCGGCATAGGCCGAGCCGTGATGTACCTGTATAAGCACCCAAAGGAATCTCGCTCTAATAAGGACAT GGCCGGCAAACTGATCAATGAGTGGAGTAGACCCATTTTTGGTTTGACCTCCAATTACAAGGGGATGACA AGAGAGGAGCGGGAACAACGCGACCTGGAACAGATGCCCCAGAGACGGCGGATGAATAGTACCGGTGGGC AGACACCACGCAGAGATCTTGAGAAAGTGCTGACCGGAGAGGAGAAAGCCCTCAGACCGGGTGATCCGGG ATTCTGTGCCAGGGCCCGGGTGCCCATGCCCAGCAATAAGGATTACGTGGTCCGCCCTAAATGGAACGTT GAGATGGAGAGCAGTCGCTTCCAAGCCACTAGTAAGAAGGGTATTTCACGGCTGGACAAACAGATGCGCA AATTTACCGACATCAGAAAGAAGTCTCGGTCTGCTCATGCTGTTAAGATTTCCATCGAGGGCAATAAGAT GCCACTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG215212 representing NM_017969
Red=Cloning site Green=Tags(s) MDSEYYSGDQSDDGGATPVQDERDSGSDGEDDVNEQHSGSDTGSVERHSENETSDREDGLPKGHHVTDSE NDEPLNLNASDSESEELHRQKDSDSESEERAEPPASDSENEDVNQHGSDSESEETRKLPGSDSENEELLN GHASDSENEDVGKHPASDSEIEELQKSPASDSETEDALKPQISDSESEEPPRHQASDSENEEPPKPRMSD SESEELPKPQVSDSESEEPPRHQASDSENEELPKPRISDSESEDPPRHQASDSENEELPKPRISDSESED PPRNQASDSENEELPKPRVSDSESEGPQKGPASDSETEDASRHKQKPESDDDSDRENKGEDTEMQNDSFH SDSHMDRKKFHSSDSEEEEHKKQKMDSDEDEKEGEEEKVAKRKAAVLSDSEDEEKASAKKSRVVSDADDS DSDAVSDKSGKREKTIASDSEEEAGKELSDKKNEEKDLFGSDSESGNEEENLIADIFGESGDEEEEEFTG FNQEDLEEEKGETQVKEAEDSDSDDNIKRGKHMDFLSDFEMMLQRKKSMSGKRRRNRDGGTFISDADDVV SAMIVKMNEAAEEDRQLNNQKKPALKKLTLLPAVVMHLKKQDLKETFIDSGVMSAIKEWLSPLPDRSLPA LKIREELLKILQELPSVSQETLKHSGIGRAVMYLYKHPKESRSNKDMAGKLINEWSRPIFGLTSNYKGMT REEREQRDLEQMPQRRRMNSTGGQTPRRDLEKVLTGEEKALRPGDPGFCARARVPMPSNKDYVVRPKWNV EMESSRFQATSKKGISRLDKQMRKFTDIRKKSRSAHAVKISIEGNKMPL TRTRRLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_017969 |
ORF Size | 2457 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Reference Data | |
RefSeq | NM_017969.1, NP_060439.1 |
RefSeq Size | 2973 |
RefSeq ORF | 2460 |
Locus ID | 55677 |
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