ADRM1 (NM_007002) Human Tagged ORF Clone

CAT#: RC215635

ADRM1 (Myc-DDK-tagged)-Human adhesion regulating molecule 1 (ADRM1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY402072) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215635 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ADRM1 protein (Cat# TP315635). The protein was produced from HEK293T cells transfected with ADRM1 cDNA clone (Cat# RC215635) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ADRM1
• Synonyms: ARM-1; ARM1; GP110
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215635 representing NM_007002
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGACCTCAGGCGCGCTCTTTCCAAGCCTGGTGCCAGGCTCTCGGGGCGCCTCCAACAAGTACTTGG
  TGGAGTTTCGGGCGGGAAAGATGTCCCTGAAGGGGACCACCGTGACTCCGGATAAGCGGAAAGGGCTGGT
  GTACATTCAGCAGACGGACGACTCGCTTATTCACTTCTGCTGGAAGGACAGGACGTCCGGGAACGTGGAA
  GACGACTTGATCATCTTCCCTGACGACTGTGAGTTCAAGCGGGTGCCGCAGTGCCCCAGCGGGAGGGTCT
  ACGTGCTGAAGTTCAAGGCAGGGTCCAAGCGGCTTTTCTTCTGGATGCAGGAACCCAAGACAGACCAGGA
  TGAGGAGCATTGCCGGAAAGTCAACGAGTATCTGAACAACCCCCCGATGCCTGGGGCACTGGGGGCCAGC
  GGAAGCAGCGGCCACGAACTCTCTGCGCTAGGCGGTGAGGGTGGCCTGCAGAGCCTGCTGGGAAACATGA
  GCCACAGCCAGCTCATGCAGCTCATCGGACCAGCCGGCCTCGGAGGACTGGGTGGGCTGGGGGCCCTGAC
  TGGACCTGGCCTGGCCAGCTTACTGGGGAGCAGTGGGCCTCCAGGGAGCAGCTCCTCCTCCAGCTCCCGG
  AGCCAGTCGGCAGCGGTCACCCCGTCATCCACCACCTCTTCCACCCGTGCCACCCCAGCCCCTTCTGCTC
  CAGCAGCTGCCTCAGCAACTAGCCCGAGCCCCGCGCCCAGTTCCGGGAATGGAGCCAGCACAGCAGCCAG
  CCCGACCCAGCCCATCCAGCTGAGCGACCTCCAGAGCATCCTGGCCACGATGAACGTACCAGCCGGGCCA
  GCAGGCGGCCAGCAAGTGGACCTGGCCAGTGTGCTGACGCCGGAGATAATGGCTCCCATCCTCGCCAACG
  CGGATGTCCAGGAGCGCCTGCTTCCCTACTTGCCATCTGGGGAGTCGCTGCCGCAGACCGCGGATGAGAT
  CCAGAATACCCTGACCTCGCCCCAGTTCCAGCAGGCCCTGGGCATGTTCAGCGCAGCCTTGGCCTCGGGG
  CAGCTGGGCCCCCTCATGTGCCAGTTCGGTCTGCCTGCAGAGGCTGTGGAGGCCGCCAACAAGGGCGATG
  TGGAAGCGTTTGCCAAAGCCATGCAGAACAACGCCAAGCCCGAGCAGAAAGAGGGCGACACGAAGGACAA
  GAAGGACGAAGAGGAGGACATGAGCCTGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215635 representing NM_007002
  Red=Cloning site Green=Tags(s)
  
  MTTSGALFPSLVPGSRGASNKYLVEFRAGKMSLKGTTVTPDKRKGLVYIQQTDDSLIHFCWKDRTSGNVE
  DDLIIFPDDCEFKRVPQCPSGRVYVLKFKAGSKRLFFWMQEPKTDQDEEHCRKVNEYLNNPPMPGALGAS
  GSSGHELSALGGEGGLQSLLGNMSHSQLMQLIGPAGLGGLGGLGALTGPGLASLLGSSGPPGSSSSSSSR
  SQSAAVTPSSTTSSTRATPAPSAPAAASATSPSPAPSSGNGASTAASPTQPIQLSDLQSILATMNVPAGP
  AGGQQVDLASVLTPEIMAPILANADVQERLLPYLPSGESLPQTADEIQNTLTSPQFQQALGMFSAALASG
  QLGPLMCQFGLPAEAVEAANKGDVEAFAKAMQNNAKPEQKEGDTKDKKDEEEDMSLD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007002
• ORF Size: 1221 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007002.1, NM_007002.2, NM_007002.3, NP_008933.2
• RefSeq Size: 1410 bp
• RefSeq ORF: 1224 bp
• Locus ID: 11047
• Domains: ARM_1
• MW: 42.15 kDa
• Gene Summary: This gene encodes a member of the adhesion regulating molecule 1 protein family. The encoded protein is a component of the proteasome where it acts as a ubiquitin receptor and recruits the deubiquitinating enzyme, ubiquitin carboxyl-terminal hydrolase L5. Increased levels of the encoded protein are associated with increased cell adhesion, which is likely an indirect effect of this intracellular protein. Dysregulation of this gene has been implicated in carcinogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]