TLR6 (NM_006068) Human Tagged ORF Clone

CAT#: RC215761

TLR6 (Myc-DDK-tagged)-Human toll-like receptor 6 (TLR6)

Product Images

Western blot validation of overexpression lysate (Cat# LY416881) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215761 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TLR6
• Synonyms: CD286
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215761 representing NM_006068
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCAAAGACAAAGAACCTATTGTTAAAAGCTTCCATTTTGTTTGCCTTATGATCATAATAGTTGGAA
  CCAGAATCCAGTTCTCCGACGGAAATGAATTTGCAGTAGACAAGTCAAAAAGAGGTCTTATTCATGTTCC
  AAAAGACCTACCGCTGAAAACCAAAGTCTTAGATATGTCTCAGAACTACATCGCTGAGCTTCAGGTCTCT
  GACATGAGCTTTCTATCAGAGTTGACAGTTTTGAGACTTTCCCATAACAGAATCCAGCTACTTGATTTAA
  GTGTTTTCAAGTTCAACCAGGATTTAGAATATTTGGATTTATCTCATAATCAGTTGCAAAAGATATCCTG
  CCATCCTATTGTGAGTTTCAGGCATTTAGATCTCTCATTCAATGATTTCAAGGCCCTGCCCATCTGTAAG
  GAATTTGGCAACTTATCACAACTGAATTTCTTGGGATTGAGTGCTATGAAGCTGCAAAAATTAGATTTGC
  TGCCAATTGCTCACTTGCATCTAAGTTATATCCTTCTGGATTTAAGAAATTATTATATAAAAGAAAATGA
  GACAGAAAGTCTACAAATTCTGAATGCAAAAACCCTTCACCTTGTTTTTCACCCAACTAGTTTATTCGCT
  ATCCAAGTGAACATATCAGTTAATACTTTAGGGTGCTTACAACTGACTAATATTAAATTGAATGATGACA
  ACTGTCAAGTTTTCATTAAATTTTTATCAGAACTCACCAGAGGTTCAACCTTACTGAATTTTACCCTCAA
  CCACATAGAAACGACTTGGAAATGCCTGGTCAGAGTCTTTCAATTTCTTTGGCCCAAACCTGTGGAATAT
  CTCAATATTTACAATTTAACAATAATTGAAAGCATTCGTGAAGAAGATTTTACTTATTCTAAAACGACAT
  TGAAAGCATTGACAATAGAACATATCACGAACCAAGTTTTTCTGTTTTCACAGACAGCTTTGTACACCGT
  GTTTTCTGAGATGAACATTATGATGTTAACCATTTCAGATACACCTTTTATACACATGCTGTGTCCTCAT
  GCACCAAGCACATTCAAGTTTTTGAACTTTACCCAGAACGTTTTCACAGATAGTATTTTTGAAAAATGTT
  CCACGTTAGTTAAATTGGAGACACTTATCTTACAAAAAAATGGATTAAAAGACCTTTTCAAAGTAGGTCT
  CATGACGAAGGATATGCCTTCTTTGGAAATACTGGATGTTAGCTGGAACTCTTTGGAATCTGGTAGACAT
  AAAGAAAACTGCACTTGGGTTGAGAGTATAGTGGTGTTAAATTTGTCTTCAAATATGCTTACTGACTCTG
  TTTTCAGATGTTTACCTCCCAGGATCAAGGTACTTGATCTTCACAGCAATAAAATAAAGAGCGTTCCTAA
  ACAAGTCGTAAAACTGGAAGCTTTGCAAGAACTCAATGTTGCTTTCAATTCTTTAACTGACCTTCCTGGA
  TGTGGCAGCTTTAGCAGCCTTTCTGTATTGATCATTGATCACAATTCAGTTTCCCACCCATCGGCTGATT
  TCTTCCAGAGCTGCCAGAAGATGAGGTCAATAAAAGCAGGGGACAATCCATTCCAATGTACCTGTGAGCT
  AAGAGAATTTGTCAAAAATATAGACCAAGTATCAAGTGAAGTGTTAGAGGGCTGGCCTGATTCTTATAAG
  TGTGACTACCCAGAAAGTTATAGAGGAAGCCCACTAAAGGACTTTCACATGTCTGAATTATCCTGCAACA
  TAACTCTGCTGATCGTCACCATCGGTGCCACCATGCTGGTGTTGGCTGTGACTGTGACCTCCCTCTGCAT
  CTACTTGGATCTGCCCTGGTATCTCAGGATGGTGTGCCAGTGGACCCAGACTCGGCGCAGGGCCAGGAAC
  ATACCCTTAGAAGAACTCCAAAGAAACCTCCAGTTTCATGCTTTTATTTCATATAGTGAACATGATTCTG
  CCTGGGTGAAAAGTGAATTGGTACCTTACCTAGAAAAAGAAGATATACAGATTTGTCTTCATGAGAGGAA
  CTTTGTCCCTGGCAAGAGCATTGTGGAAAATATCATCAACTGCATTGAGAAGAGTTACAAGTCCATCTTT
  GTTTTGTCTCCCAACTTTGTCCAGAGTGAGTGGTGCCATTACGAACTCTATTTTGCCCATCACAATCTCT
  TTCATGAAGGATCTAATAACTTAATCCTCATCTTACTGGAACCCATTCCACAGAACAGCATTCCCAACAA
  GTACCACAAGCTGAAGGCTCTCATGACGCAGCGGACTTATTTGCAGTGGCCCAAGGAGAAAAGCAAACGT
  GGGCTCTTTTGGGCTAACATTAGAGCCGCTTTTAATATGAAATTAACACTAGTCACTGAAAACAATGATG
  TGAAATCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215761 representing NM_006068
  Red=Cloning site Green=Tags(s)
  
  MTKDKEPIVKSFHFVCLMIIIVGTRIQFSDGNEFAVDKSKRGLIHVPKDLPLKTKVLDMSQNYIAELQVS
  DMSFLSELTVLRLSHNRIQLLDLSVFKFNQDLEYLDLSHNQLQKISCHPIVSFRHLDLSFNDFKALPICK
  EFGNLSQLNFLGLSAMKLQKLDLLPIAHLHLSYILLDLRNYYIKENETESLQILNAKTLHLVFHPTSLFA
  IQVNISVNTLGCLQLTNIKLNDDNCQVFIKFLSELTRGSTLLNFTLNHIETTWKCLVRVFQFLWPKPVEY
  LNIYNLTIIESIREEDFTYSKTTLKALTIEHITNQVFLFSQTALYTVFSEMNIMMLTISDTPFIHMLCPH
  APSTFKFLNFTQNVFTDSIFEKCSTLVKLETLILQKNGLKDLFKVGLMTKDMPSLEILDVSWNSLESGRH
  KENCTWVESIVVLNLSSNMLTDSVFRCLPPRIKVLDLHSNKIKSVPKQVVKLEALQELNVAFNSLTDLPG
  CGSFSSLSVLIIDHNSVSHPSADFFQSCQKMRSIKAGDNPFQCTCELREFVKNIDQVSSEVLEGWPDSYK
  CDYPESYRGSPLKDFHMSELSCNITLLIVTIGATMLVLAVTVTSLCIYLDLPWYLRMVCQWTQTRRRARN
  IPLEELQRNLQFHAFISYSEHDSAWVKSELVPYLEKEDIQICLHERNFVPGKSIVENIINCIEKSYKSIF
  VLSPNFVQSEWCHYELYFAHHNLFHEGSNNLILILLEPIPQNSIPNKYHKLKALMTQRTYLQWPKEKSKR
  GLFWANIRAAFNMKLTLVTENNDVKS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006068
• ORF Size: 2388 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006068.1, NM_006068.2, NM_006068.3, NM_006068.4, NP_006059.2
• RefSeq Size: 2753 bp
• RefSeq ORF: 2391 bp
• Locus ID: 10333
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Toll-like receptor signaling pathway
• MW: 91.7 kDa
• Gene Summary: The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor functionally interacts with toll-like receptor 2 to mediate cellular response to bacterial lipoproteins. A Ser249Pro polymorphism in the extracellular domain of the encoded protein may be associated with an increased of asthma is some populations. [provided by RefSeq, Jan 2011]