SARM (SARM1) (NM_015077) Human Tagged ORF Clone

CAT#: RC215903

SARM1 (Myc-DDK-tagged)-Human sterile alpha and TIR motif containing 1 (SARM1)

Product Images

Coomassie blue staining of purified SARM1 protein (Cat# TP315903). The protein was produced from HEK293T cells transfected with SARM1 cDNA clone (Cat# RC215903) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SARM1
• Synonyms: hSARM1; HsTIR; MyD88-5; SAMD2; SARM
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215903 representing NM_015077.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGTCCTGACGCTGCTTCTCTCCGCCTACAAGCTGTGTCGCTTCTTCGCCATGTCGGGCCCACGGCCG
  GGCGCCGAGCGGCTGGCGGTGCCTGGGCCAGATGGGGGCGGTGGCACGGGCCCATGGTGGGCTGCGGGT
  GGCCGCGGGCCCCGCGAAGTGTCGCCGGGGGCAGGCACCGAGGTGCAGGACGCCCTGGAGCGCGCGCTG
  CCGGAGCTGCAGCAGGCCTTGTCCGCGCTGAAGCAGGCGGGCGGCGCGCGGGCCGTGGGCGCCGGCCTG
  GCCGAGGTCTTCCAACTGGTGGAGGAGGCCTGGCTGCTGCCGGCCGTGGGCCGCGAGGTAGCCCAGGGT
  CTGTGCGACGCCATCCGCCTCGATGGCGGCCTCGACCTGCTGTTGCGGCTGCTGCAGGCGCCGGAGTTG
  GAGACGCGTGTGCAGGCCGCGCGCCTGCTGGAGCAGATCCTGGTGGCTGAGAACCGAGACCGCGTGGCG
  CGCATTGGGCTGGGCGTGATCCTGAACCTGGCGAAGGAACGCGAACCCGTAGAGCTGGCGCGGAGCGTG
  GCAGGCATCTTGGAGCACATGTTCAAGCATTCGGAGGAGACATGCCAGAGGCTGGTGGCGGCCGGCGGC
  CTGGACGCGGTGCTGTATTGGTGCCGCCGCACGGACCCCGCGCTGCTGCGCCACTGCGCGCTGGCGCTG
  GGCAACTGCGCGCTGCACGGGGGCCAGGCGGTGCAGCGACGCATGGTAGAGAAGCGCGCAGCCGAGTGG
  CTCTTCCCGCTCGCCTTCTCCAAGGAGGACGAGCTGCTTCGGCTGCACGCCTGCCTCGCAGTAGCGGTG
  TTGGCGACTAACAAGGAGGTGGAGCGCGAGGTGGAGCGCTCGGGCACGCTGGCGCTCGTGGAGCCGCTT
  GTGGCCTCGCTGGACCCTGGCCGCTTCGCCCGCTGTCTGGTGGACGCCAGCGACACAAGCCAGGGCCGC
  GGGCCCGACGACCTGCAGCGCCTCGTGCCGTTGCTCGACTCTAACCGCTTGGAGGCGCAGTGCATCGGG
  GCTTTCTACCTCTGCGCCGAGGCTGCCATCAAGAGCCTGCAAGGCAAGACCAAGGTGTTCAGCGACATC
  GGCGCCATCCAGAGCCTGAAACGCCTGGTTTCCTACTCTACCAATGGCACTAAGTCGGCGCTGGCCAAG
  CGCGCGCTGCGCCTGCTGGGCGAGGAGGTGCCACGGCCCATCCTGCCCTCCGTGCCCAGCTGGAAGGAG
  GCCGAGGTTCAGACGTGGCTGCAGCAGATCGGTTTCTCCAAGTACTGCGAGAGCTTCCGGGAGCAGCAG
  GTGGATGGCGACCTGCTTCTGCGGCTCACGGAGGAGGAACTCCAGACCGACCTGGGCATGAAATCGGGC
  ATCACCCGCAAGAGGTTCTTTAGGGAGCTCACGGAGCTCAAGACCTTCGCCAACTATTCTACGTGCGAC
  CGCAGCAACCTGGCGGACTGGCTGGGCAGCCTGGACCCGCGCTTCCGCCAGTACACCTACGGCCTGGTC
  AGCTGCGGCCTGGACCGCTCCCTGCTGCACCGCGTGTCTGAGCAGCAGCTGCTGGAAGACTGCGGCATC
  CACCTGGGCGTGCACCGCGCCCGCATCCTCACGGCGGCCAGAGAAATGCTACACTCCCCGCTGCCCTGT
  ACTGGTGGCAAACCCAGTGGGGACACTCCAGATGTCTTCATCAGCTACCGCCGGAACTCAGGTTCCCAG
  CTGGCCAGTCTCCTGAAGGTGCACCTGCAGCTGCATGGCTTCAGTGTCTTCATTGATGTGGAGAAGCTG
  GAAGCAGGCAAGTTCGAGGACAAACTCATCCAGAGTGTCATGGGTGCCCGCAACTTTGTGTTGGTGCTA
  TCACCTGGAGCACTGGACAAGTGCATGCAAGACCATGACTGCAAGGATTGGGTGCATAAGGAGATTGTG
  ACTGCTTTAAGCTGCGGCAAGAACATTGTGCCCATCATTGATGGCTTCGAGTGGCCTGAGCCCCAGGTC
  CTGCCTGAGGACATGCAGGCTGTGCTTACTTTCAACGGTATCAAGTGGTCCCACGAATACCAGGAGGCC
  ACCATTGAGAAGATCATCCGCTTCCTGCAGGGCCGCTCCTCCCGGGACTCATCTGCAGGCTCTGACACC
  AGTTTGGAGGGTGCTGCACCCATGGGTCCAACC
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
  Protein Sequence

  >Peptide sequence encoded by RC215903
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MVLTLLLSAYKLCRFFAMSGPRPGAERLAVPGPDGGGGTGPWWAAGGRGPREVSPGAGTEVQDALERAL
  PELQQALSALKQAGGARAVGAGLAEVFQLVEEAWLLPAVGREVAQGLCDAIRLDGGLDLLLRLLQAPEL
  ETRVQAARLLEQILVAENRDRVARIGLGVILNLAKEREPVELARSVAGILEHMFKHSEETCQRLVAAGG
  LDAVLYWCRRTDPALLRHCALALGNCALHGGQAVQRRMVEKRAAEWLFPLAFSKEDELLRLHACLAVAV
  LATNKEVEREVERSGTLALVEPLVASLDPGRFARCLVDASDTSQGRGPDDLQRLVPLLDSNRLEAQCIG
  AFYLCAEAAIKSLQGKTKVFSDIGAIQSLKRLVSYSTNGTKSALAKRALRLLGEEVPRPILPSVPSWKE
  AEVQTWLQQIGFSKYCESFREQQVDGDLLLRLTEEELQTDLGMKSGITRKRFFRELTELKTFANYSTCD
  RSNLADWLGSLDPRFRQYTYGLVSCGLDRSLLHRVSEQQLLEDCGIHLGVHRARILTAAREMLHSPLPC
  TGGKPSGDTPDVFISYRRNSGSQLASLLKVHLQLHGFSVFIDVEKLEAGKFEDKLIQSVMGARNFVLVL
  SPGALDKCMQDHDCKDWVHKEIVTALSCGKNIVPIIDGFEWPEPQVLPEDMQAVLTFNGIKWSHEYQEA
  TIEKIIRFLQGRSSRDSSAGSDTSLEGAAPMGPT
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
  Recombinant protein using RC215903 also available, TP315903M
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015077
• ORF Size: 2172 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015077.4
• RefSeq Size: 7001 bp
• RefSeq ORF: 2175 bp
• Locus ID: 23098
• UniProt ID: Q6SZW1
• Cytogenetics: 17q11.2
• Domains: TIR, SAM
• Protein Families: Druggable Genome
• MW: 79.4 kDa
• Gene Summary: Negative regulator of MYD88- and TRIF-dependent toll-like receptor signaling pathway which plays a pivotal role in activating axonal degeneration following injury. Promotes Wallerian degeneration an injury-induced axonal death pathway which involves degeneration of an axon distal to the injury site. Can activate neuronal death in response to stress. Regulates dendritic arborization through the MAPK4-JNK pathway. Involved in innate immune response. Inhibits both TICAM1/TRIF- and MYD88-dependent activation of JUN/AP-1, TRIF-dependent activation of NF-kappa-B and IRF3, and the phosphorylation of MAPK14/p38.[UniProtKB/Swiss-Prot Function]