PACAP receptor (ADCYAP1R1) (NM_001118) Human Tagged ORF Clone

CAT#: RC215923

ADCYAP1R1 (Myc-DDK-tagged)-Human adenylate cyclase activating polypeptide 1 (pituitary) receptor type I (ADCYAP1R1), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ADCYAP1R1
• Synonyms: PAC1; PAC1R; PACAPR; PACAPRI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215923 representing NM_001118
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGTGTCGTGCACGTTTCCCTGGCTGCTCTCCTCCTGCTGCCTATGGCCCCTGCCATGCATTCTG
  ACTGCATCTTCAAGAAGGAGCAAGCCATGTGCCTGGAGAAGATCCAGAGGGCCAATGAGCTGATGGGCTT
  CAATGATTCCTCTCCAGGCTGTCCTGGGATGTGGGACAACATCACGTGTTGGAAGCCCGCCCATGTGGGT
  GAGATGGTCCTGGTCAGCTGCCCTGAGCTCTTCCGAATCTTCAACCCAGACCAAGTCTGGGAGACCGAAA
  CCATTGGAGAGTCTGATTTTGGTGACAGTAACTCCTTAGATCTCTCAGACATGGGAGTGGTGAGCCGGAA
  CTGCACGGAGGATGGCTGGTCGGAACCCTTCCCTCATTACTTTGATGCCTGTGGGTTTGATGAATATGAA
  TCTGAGACTGGGGACCAGGATTATTACTACCTGTCAGTGAAGGCCCTCTACACGGTTGGCTACAGCACAT
  CCCTCGTCACCCTCACCACTGCCATGGTCATCCTTTGTCGCTTCCGGAAGCTGCACTGCACACGCAACTT
  CATCCACATGAACCTGTTTGTGTCGTTCATGCTGAGGGCGATCTCCGTCTTCATCAAAGACTGGATTCTG
  TATGCGGAGCAGGACAGCAACCACTGCTTCATCTCCACTGTGGAATGTAAGGCCGTCATGGTTTTCTTCC
  ACTACTGTGTTGTGTCCAACTACTTCTGGCTGTTCATCGAGGGCCTGTACCTCTTCACTCTGCTGGTGGA
  GACCTTCTTCCCTGAAAGGAGATACTTCTACTGGTACACCATCATTGGCTGGGGGACCCCAACTGTGTGT
  GTGACAGTGTGGGCTACGCTGAGACTCTACTTTGATGACACAGGCTGCTGGGATATGAATGACAGCACAG
  CTCTGTGGTGGGTGATCAAAGGCCCTGTGGTTGGCTCTATCATGGTTAACTTTGTGCTTTTTATTGGCAT
  TATCGTCATCCTTGTGCAGAAACTTCAGTCTCCAGACATGGGAGGCAATGAGTCCAGCATCTACTTGCGA
  CTGGCCCGGTCCACCCTGCTGCTCATCCCACTATTCGGAATCCACTACACAGTATTTGCCTTCTCCCCAG
  AGAATGTCAGCAAAAGGGAAAGACTCGTGTTTGAGCTGGGGCTGGGCTCCTTCCAGGGCTTTGTGGTGGC
  TGTTCTCTACTGTTTTCTGAATGGTGAGGTACAAGCGGAGATCAAGCGAAAATGGCGAAGCTGGAAGGTG
  AACCGTTACTTCGCTGTGGACTTCAAGCACCGACACCCGTCTCTGGCCAGCAGTGGGGTGAATGGGGGCA
  CCCAGCTCTCCATCCTGAGCAAGAGCAGCTCCCAAATCCGCATGTCTGGCCTCCCTGCTGACAATCTGGC
  CACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215923 representing NM_001118
  Red=Cloning site Green=Tags(s)
  
  MAGVVHVSLAALLLLPMAPAMHSDCIFKKEQAMCLEKIQRANELMGFNDSSPGCPGMWDNITCWKPAHVG
  EMVLVSCPELFRIFNPDQVWETETIGESDFGDSNSLDLSDMGVVSRNCTEDGWSEPFPHYFDACGFDEYE
  SETGDQDYYYLSVKALYTVGYSTSLVTLTTAMVILCRFRKLHCTRNFIHMNLFVSFMLRAISVFIKDWIL
  YAEQDSNHCFISTVECKAVMVFFHYCVVSNYFWLFIEGLYLFTLLVETFFPERRYFYWYTIIGWGTPTVC
  VTVWATLRLYFDDTGCWDMNDSTALWWVIKGPVVGSIMVNFVLFIGIIVILVQKLQSPDMGGNESSIYLR
  LARSTLLLIPLFGIHYTVFAFSPENVSKRERLVFELGLGSFQGFVVAVLYCFLNGEVQAEIKRKWRSWKV
  NRYFAVDFKHRHPSLASSGVNGGTQLSILSKSSSQIRMSGLPADNLAT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001118
• ORF Size: 1404 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001118.2, NM_001118.3, NM_001118.4, NP_001109.2
• RefSeq Size: 1643 bp
• RefSeq ORF: 1407 bp
• Locus ID: 117
• Cytogenetics: 7p14.3
• Domains: 7tm_2, HormR
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 53.31 kDa
• Gene Summary: 'This gene encodes type I adenylate cyclase activating polypeptide receptor, which is a membrane-associated protein and shares significant homology with members of the glucagon/secretin receptor family. This receptor mediates diverse biological actions of adenylate cyclase activating polypeptide 1 and is positively coupled to adenylate cyclase. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Dec 2010]'