LRRFIP1 (NM_004735) Human Tagged ORF Clone

CAT#: RC216265

LRRFIP1 (Myc-DDK-tagged)-Human leucine rich repeat (in FLII) interacting protein 1 (LRRFIP1), transcript variant 4

Product Images

Western blot validation of overexpression lysate (Cat# LY417786) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216265 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified LRRFIP1 protein (Cat# TP316265). The protein was produced from HEK293T cells transfected with LRRFIP1 cDNA clone (Cat# RC216265) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LRRFIP1
• Synonyms: FLAP-1; FLAP1; FLIIAP1; GCF-2; GCF2; HUFI-1; TRIP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216265 representing NM_004735
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCAGCCCCGCGGCCGCTCAAAGCCGGGAGATCGACTGTTTGAGCCCGGAAGCGCAGAAGCTGGCGG
  AAGCCCGGCTCGCTGCAAAACGGGCGGCCCGCGCGGAGGCTCGCGAGATCCGCATGAAGGAGCTGGAGCG
  GCAGCAGAAGGAGGAAGACAGTGAGCGCTACTCTCGTAGATCCAGAAGAAACACATCGGCTTCTGATGAA
  GACGAGCGCATGTCAGTGGGTAGTCGTGGAAGCCTGAGGGTAGAAGAGAGACCAGAAAAAGATTTTACTG
  AGAAGGGGTCTCGTAACATGCCGGGCCTGTCTGCAGCCACGCTGGCCTCTCTGGGTGGGACTTCCTCTCG
  GAGAGGCAGCGGAGACACCTCCATCTCCATCGACACCGAGGCATCCATCAGGGAAATCAAGGACTCTCTA
  GCAGAAGTTGAAGAGAAATATAAGAAGGCTATGGTTTCCAATGCTCAGCTAGACAATGAAAAGACAAACT
  TCATGTACCAGGTTGATACCCTAAAAGATATGTTGCTGGAGCTTGAAGAACAGCTGGCTGAATCTAGGCG
  GCAGTACGAAGAGAAAAACAAAGAATTTGAAAGGGAAAAACACGCCCACAGTATACTGCAATTTCAGTTT
  GCTGAAGTCAAGGAGGCCCTGAAGCAAAGAGAGGAAATGCTCGAGAAACATGGAATAATCCTAAATTCAG
  AAATAGCTACCAATGGAGAGACTTCCGACACCCTCAATAATGTTGGATACCAAGGTCCTACCAAGATGAC
  AAAAGAAGAGTTAAATGCCCTCAAGTCGACAGGGGATGGGACCCTAGGAAGAGCCAGTGAAGTGGAGGTG
  AAAAATGAAATCGTGGCGAATGTGGGGAAAAGAGAAATCTTGCACAATACTGAGAAAGAACAACACACAG
  AGGACACAGTGAAGGACTGTGTGGACATAGAGGTATTCCCTGCTGGTGAGAATACCGAGGACCAGAAATC
  CTCTGAAGACACTGCCCCATTCCTAGGAACCTTAGCAGGTGCTACCTATGAGGAACAGGTTCAAAGCCAA
  ATTCTTGAGAGCAGTTCTCTCCCTGAAAACACAGTACAGGTTGAGTCAAATGAGGTCATGGGTGCACCAG
  ATGACAGGACCAGAACTCCCCTTGAGCCATCCAACTGTTGGAGTGACTTAGATGGTGGGAACCACACAGA
  GAATGTGGGAGAGGCAGCAGTGACTCAGGTTGAAGAGCAGGCAGGCACAGTGGCCTCGTGTCCTTTAGGG
  CATAGTGATGACACAGTTTATCATGATGACAAATGTATGGTAGAGGTCCCCCAAGAGTTAGAGACAAGCA
  CAGGGCATAGTTTAGAGAAAGAATTCACCAACCAGGAAGCAGCTGAGCCCAAGGAGGTTCCAGCGCACAG
  TACAGAAGTAGGTAGGGATCACAACGAAGAAGAGGGTGAAGAAACAGGATTAAGGGACGAGAAACCAATC
  AAGACAGAAGTTCCTGGTTCTCCAGCAGGAACTGAGGGCAACTGTCAGGAAGCGACAGGTCCAAGTACAG
  TAGACACTCAAAATGAACCCTTAGATATGAAAGAGCCCGATGAAGAAAAGAGTGACCAACAGGGAGAGGC
  ATTGGACTCATCGCAGAAGAAGACAAAGAACAAGAAAAAGAAAAACAAGAAGAAAAAATCCCCAGTACCC
  GTAGAAACCCTTAAAGATGTTAAAAAAGAGTTAACGTATCAGAACACAGATTTAAGTGAAATTAAGGAAG
  AAGAGCAGGTAAAGTCTACTGACAGAAAGTCAGCAGTGGAAGCCCAAAACGAGGTGACTGAAAATCCAAA
  ACAGAAAATTGCAGCAGAAAGCAGTGAAAATGTTGATTGTCCGGAGAATCCTAAAATTAAGTTGGATGGA
  AAACTTGACCAAGAAGGTGATGATGTACAAACAGCAGCTGAGGAGGTACTAGCTGATGGAGACACATTAG
  ATTTTGAGGATGACACCGTTCAATCATCAGGCCCGAGGGCTGGTGGTGAAGAATTAGATGAAGGTGTTGC
  AAAAGATAATGCTAAAATAGATGGTGCCACTCAAAGCAGTCCTGCAGAACCAAAGAGCGAAGACGCAGAT
  CGCTGCACCCTGCCCGAACATGAAAGTCCCTCACAGGACATTAGTGATGCCTGTGAAGCAGAAAGTACAG
  AGAGGTGTGAGATGTCAGAACATCCAAGTCAGACCGTCAGGAAAGCTTTAGACAGCAATAGCCTAGAGAA
  CGATGACTTGTCGGCACCAGGAAGAGAGCCAGGGCACTTCAATCCAGAAAGCAGAGAAGATACCAGAGGA
  GGGAATGAGAAGGGCAAAAGCAAAGAAGACTGTACCATGTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216265 representing NM_004735
  Red=Cloning site Green=Tags(s)
  
  MTSPAAAQSREIDCLSPEAQKLAEARLAAKRAARAEAREIRMKELERQQKEEDSERYSRRSRRNTSASDE
  DERMSVGSRGSLRVEERPEKDFTEKGSRNMPGLSAATLASLGGTSSRRGSGDTSISIDTEASIREIKDSL
  AEVEEKYKKAMVSNAQLDNEKTNFMYQVDTLKDMLLELEEQLAESRRQYEEKNKEFEREKHAHSILQFQF
  AEVKEALKQREEMLEKHGIILNSEIATNGETSDTLNNVGYQGPTKMTKEELNALKSTGDGTLGRASEVEV
  KNEIVANVGKREILHNTEKEQHTEDTVKDCVDIEVFPAGENTEDQKSSEDTAPFLGTLAGATYEEQVQSQ
  ILESSSLPENTVQVESNEVMGAPDDRTRTPLEPSNCWSDLDGGNHTENVGEAAVTQVEEQAGTVASCPLG
  HSDDTVYHDDKCMVEVPQELETSTGHSLEKEFTNQEAAEPKEVPAHSTEVGRDHNEEEGEETGLRDEKPI
  KTEVPGSPAGTEGNCQEATGPSTVDTQNEPLDMKEPDEEKSDQQGEALDSSQKKTKNKKKKNKKKKSPVP
  VETLKDVKKELTYQNTDLSEIKEEEQVKSTDRKSAVEAQNEVTENPKQKIAAESSENVDCPENPKIKLDG
  KLDQEGDDVQTAAEEVLADGDTLDFEDDTVQSSGPRAGGEELDEGVAKDNAKIDGATQSSPAEPKSEDAD
  RCTLPEHESPSQDISDACEAESTERCEMSEHPSQTVRKALDSNSLENDDLSAPGREPGHFNPESREDTRG
  GNEKGKSKEDCTMS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004735
• ORF Size: 2352 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004735.1, NM_004735.2, NM_004735.3, NP_004726.1
• RefSeq Size: 3648 bp
• RefSeq ORF: 2355 bp
• Locus ID: 9208
• Protein Families: Transcription Factors
• MW: 86.2 kDa