ARHGEF4 (NM_032995) Human Tagged ORF Clone

CAT#: RC216845

ARHGEF4 (Myc-DDK-tagged)-Human Rho guanine nucleotide exchange factor (GEF) 4 (ARHGEF4), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY409795) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216845 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ARHGEF4
• Synonyms: ASEF; ASEF1; GEF4; STM6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216845 representing NM_032995
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCTGGGAAGAACCAGCAGGTGAGAAGCCCAGTTGCTCTCACAGTCAGAAGGCGTTCCACATGGAGC
  CTGCCCAGAAGCCCTGCTTCACCACTGACATGGTGACATGGGCCCTCCTCTGCATCTCTGCAGAGACTGT
  GCGTGGGGAGGCTCCTTCACAGCCTAGGGGCATCCCTCACCGCTCGCCCGTCAGTGTGGATGACCTGTGG
  CTGGAGAAGACACAGAGAAAGAAGTTGCAGAAGCAGGCCCACGTCGAAAGGAGGCTGCACATAGGGGCAG
  TGCACAAAGATGGAGTCAAGTGCTGGAGAAAGACGATCATTACCTCTCCAGAGTCTTTGAATCTCCCTAG
  AAGAAGCCATCCACTCTCCCAGAGTGCTCCAACGGGACTGAACCACATGGGCTGGCCAGAGCACACACCA
  GGCACTGCCATGCCTGATGGAGCTCTGGACACAGCTGTCTGCGCTGACGAAGTGGGGAGCGAGGAGGACC
  TGTATGATGACCTGCACAGCTCCAGCCACCACTACAGCCACCCTGGAGGGGGTGGGGAGCAGCTGGCTAT
  CAATGAGCTCATCAGCGATGGCAGTGTGGTCTGCGCTGAAGCACTCTGGGACCATGTCACCATGGACGAC
  CAGGAGCTGGGCTTCAAAGCTGGGGACGTCATCGAAGTGATGGATGCCACCAACAGAGAGTGGTGGTGGG
  GCCGGGTCGCCGATGGCGAGGGCTGGTTTCCAGCCAGCTTCGTTCGGCTGAGGGTGAATCAGGACGAGCC
  CGCGGATGACGACGCCCCTCTGGCCGGGAACAGCGGAGCGGAGGACGGCGGGGCGGAGGCGCAGAGCAGC
  AAGGACCAGATGCGGACCAACGTCATCAACGAGATCCTCAGCACTGAGCGGGACTACATCAAGCACCTGC
  GCGACATCTGCGAGGGCTACGTCCGGCAGTGCCGCAAGCGCGCAGACATGTTCAGCGAGGAGCAGCTGCG
  TACCATCTTCGGGAACATCGAGGACATCTACCGCTGCCAGAAGGCCTTCGTGAAGGCCCTGGAGCAGAGG
  TTCAACCGCGAGCGCCCACACCTGAGCGAGCTGGGTGCCTGCTTCCTGGAGCATCAAGCCGACTTCCAGA
  TCTACTCGGAGTACTGCAATAACCACCCCAACGCCTGCGTGGAGCTCTCCCGGCTCACCAAGCTCAGCAA
  GTACGTGTACTTCTTCGAGGCCTGCCGGCTGCTGCAGAAGATGATTGACATCTCCCTGGATGGCTTCCTG
  CTGACTCCGGTGCAGAAGATCTGCAAGTACCCTCTGCAGCTGGCCGAGCTGCTCAAATACACGCACCCCC
  AGCACAGGGACTTCAAGGATGTTGAAGCCGCCTTGCATGCCATGAAGAACGTGGCCCAGCTCATCAACGA
  GCGGAAGCGGAGACTTGAGAACATCGACAAGATTGCTCAGTGGCAGAGCTCCATAGAGGACTGGGAGGGA
  GAAGATCTCTTGGTCAGGAGCTCAGAACTCATCTACTCGGGGGAGCTGACTCGAGTTACACAGCCTCAAG
  CCAAAAGCCAGCAGCGAATGTTCTTTCTCTTTGACCACCAGCTCATCTACTGTAAGAAGGACCTGCTCCG
  CCGCGACGTGTTGTACTACAAGGGCCGGCTGGACATGGACGGCCTGGAGGTGGTGGACCTGGAGGACGGG
  AAGGACAGAGACCTCCATGTGAGCATCAAGAACGCCTTCCGGCTGCACCGTGGCGCCACAGGGGACAGCC
  ACCTGCTGTGCACCAGGAAGCCCGAGCAGAAGCAGCGCTGGCTCAAGGCCTTTGCCAGGGAGAGGGAGCA
  GGTGCAGCTGGACCAGGAGACAGGCTTCTCCATCACTGAACTGCAGAGGAAGCAGGCCATGCTGAATGCC
  AGCAAGCAGCAGGTCACAGGGAAGCCCAAAGGTAGGCGGACAGCAGCCCCACCTCCTCGGCTGCCCGGTC
  CTTACCCTGCTGACATCATTCCCTTCTCTGAACCACAAAGCCAAGCCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216845 representing NM_032995
  Red=Cloning site Green=Tags(s)
  
  MPWEEPAGEKPSCSHSQKAFHMEPAQKPCFTTDMVTWALLCISAETVRGEAPSQPRGIPHRSPVSVDDLW
  LEKTQRKKLQKQAHVERRLHIGAVHKDGVKCWRKTIITSPESLNLPRRSHPLSQSAPTGLNHMGWPEHTP
  GTAMPDGALDTAVCADEVGSEEDLYDDLHSSSHHYSHPGGGGEQLAINELISDGSVVCAEALWDHVTMDD
  QELGFKAGDVIEVMDATNREWWWGRVADGEGWFPASFVRLRVNQDEPADDDAPLAGNSGAEDGGAEAQSS
  KDQMRTNVINEILSTERDYIKHLRDICEGYVRQCRKRADMFSEEQLRTIFGNIEDIYRCQKAFVKALEQR
  FNRERPHLSELGACFLEHQADFQIYSEYCNNHPNACVELSRLTKLSKYVYFFEACRLLQKMIDISLDGFL
  LTPVQKICKYPLQLAELLKYTHPQHRDFKDVEAALHAMKNVAQLINERKRRLENIDKIAQWQSSIEDWEG
  EDLLVRSSELIYSGELTRVTQPQAKSQQRMFFLFDHQLIYCKKDLLRRDVLYYKGRLDMDGLEVVDLEDG
  KDRDLHVSIKNAFRLHRGATGDSHLLCTRKPEQKQRWLKAFAREREQVQLDQETGFSITELQRKQAMLNA
  SKQQVTGKPKGRRTAAPPPRLPGPYPADIIPFSEPQSQAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032995
• ORF Size: 2010 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_032995.1, NM_032995.2, NP_127462.1
• RefSeq Size: 4258
• RefSeq ORF: 2013
• Locus ID: 50649
• Protein Families: Druggable Genome
• Protein Pathways: Regulation of actin cytoskeleton
• MW: 76.2 kDa
• Gene Summary: Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The protein encoded by this gene may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants encoding different isoforms have been found, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jun 2013]