POTEB3 (NM_207355) Human Tagged ORF Clone

CAT#: RC216900

POTEB (Myc-DDK-tagged)-Human POTE ankyrin domain family, member B (POTEB)

Product Images

Western blot validation of overexpression lysate (Cat# LY403978) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216900 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified POTEB3 protein (Cat# TP316900). The protein was produced from HEK293T cells transfected with POTEB3 cDNA clone (Cat# RC216900) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: POTEB3
• Synonyms: POTE-15; POTEB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216900 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGCTGAGGTTTGTTCAATGCCCGCTGCCTCTGCTGTGAAGAAGCCATTTGATCTCAGGAGCAAGA
  TGGGCAAGTGGTGCCACCACCGCTTCCCCTGCTGCAGGGGGAGCGGCAAGAGCAACATGGGCACTTCTGG
  AGACCACGACGACTCCTTTATGAAGACGCTCAGGAGCAAGATGGGCAAGTGTTGCCACCACTGCTTCCCC
  TGCTGCAGGGGGAGCGGCACGAGCAATGTGGGCACTTCTGGAGACCATGACAACTCCTTTATGAAGACAC
  TCAGGAGCAAGATGGGCAAGTGGTGCTGTCACTGCTTCCCCTGCTGCAGGGGGAGCGGCAAGAGCAACGT
  GGGCACTTGGGGAGACTACGACGACAGCGCCTTCATGGAGCCGAGGTACCACGTCCGTCGAGAAGATCTG
  GACAAGCTCCACAGAGCTGCCTGGTGGGGTAAAGTCCCCAGAAAGGATCTCATCGTCATGCTCAGGGACA
  CTGACATGAACAAGAGGGACAAGCAAAAGAGGACTGCTCTACATTTGGCCTCTGCCAATGGAAATTCAGA
  AGTAGTACAACTCCTGCTGGACAGACGATGTCAACTTAACGTCCTTGACAACAAAAAAAGGACAGCTCTG
  ATAAAGGCCGTACAATGCCAGGAAGATGAATGTGTGTTAATGTTGCTGGAACATGGCGCTGATGGAAATA
  TTCAAGATGAGTATGGAAATACCGCTCTACACTATGCTATCTACAATGAAGATAAATTAATGGCCAAAGC
  ACTGCTCTTATATGGTGCTGATATTGAATCAAAAAACAAGTGTGGCCTCACACCACTTTTGCTTGGCGTA
  CATGAACAAAAACAGCAAGTGGTGAAATTTTTAATCAAGAAAAAAGCTAATTTAAATGCACTTGATAGAT
  ATGGAAGAACTGCCCTCATACTTGCTGTATGTTGTGGATCAGCAAGTATAGTCAATCTTCTACTTGAGCA
  AAATGTTGATGTATCTTCTCAAGATCTATCTGGACAGACGGCCAGAGAGTATGCTGTTTCTAGTCATCAT
  CATGTAATTTGTGAATTACTTTCTGACTATAAAGAAAAACAGATGCTAAAAATCTCTTCTGAAAACAGCA
  ATCCAGAACAAGACTTAAAGCTGACATCAGAGGAAGAGTCACAAAGGCTTAAAGTCAGTGAAAATAGTCA
  GCCAGAGAAAATGTCTCAAGAACCAGAAATAAATAAGGACTGTGATAGAGAGGTTGAAGAAGAAATAAAG
  AAGCATGGAAGTAATCCTGTGGGATTACCAGAAAACCTGACTAATGGTGCCAGTGCTGGCAATGGTGATG
  ATGGATTAATTCCACAAAGGAAGAGCAGAAAACCTGAAAATCAGCAATTTCCTGACACTGAGAATGAAGA
  GTATCACAGTGACGAACAAAATGATACCCAGAAACAACTTTCTGAAGAACAGAACACTGGAATATCACAA
  GATGAGATTCTGACTAATAAACAAAAGCAGATAGAAGTGGCTGAAAAGGAAATGAATTCTGAGCTTTCTC
  TTAGTCATAAGAAAGAAGAAGATCTCTTGCGTGAAAACAGCATGTTGCGGGAAGAAATTGCCAAGCTAAG
  ACTGGAACTAGATGAAACAAAACATCAGAACCAGCTAAGGGAAAATAAAATTTTGGAGGAAATTGAAAGT
  GTAAAAGAAAAACTTCTAAAGACTATACAACTGAATGAAGAAGCATTAACGAAAACCAGTATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216900 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVAEVCSMPAASAVKKPFDLRSKMGKWCHHRFPCCRGSGKSNMGTSGDHDDSFMKTLRSKMGKCCHHCFP
  CCRGSGTSNVGTSGDHDNSFMKTLRSKMGKWCCHCFPCCRGSGKSNVGTWGDYDDSAFMEPRYHVRREDL
  DKLHRAAWWGKVPRKDLIVMLRDTDMNKRDKQKRTALHLASANGNSEVVQLLLDRRCQLNVLDNKKRTAL
  IKAVQCQEDECVLMLLEHGADGNIQDEYGNTALHYAIYNEDKLMAKALLLYGADIESKNKCGLTPLLLGV
  HEQKQQVVKFLIKKKANLNALDRYGRTALILAVCCGSASIVNLLLEQNVDVSSQDLSGQTAREYAVSSHH
  HVICELLSDYKEKQMLKISSENSNPEQDLKLTSEEESQRLKVSENSQPEKMSQEPEINKDCDREVEEEIK
  KHGSNPVGLPENLTNGASAGNGDDGLIPQRKSRKPENQQFPDTENEEYHSDEQNDTQKQLSEEQNTGISQ
  DEILTNKQKQIEVAEKEMNSELSLSHKKEEDLLRENSMLREEIAKLRLELDETKHQNQLRENKILEEIES
  VKEKLLKTIQLNEEALTKTSI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_207355
• ORF Size: 1743 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_207355.2, NM_207355.3, NP_997238.2
• RefSeq Size: 2136
• RefSeq ORF: 1746
• Locus ID: 102724631
• MW: 65.7 kDa