Cadherin 8 (CDH8) (NM_001796) Human Tagged ORF Clone

CAT#: RC217249

CDH8 (Myc-DDK-tagged)-Human cadherin 8, type 2 (CDH8)

Product Images

Western blot validation of overexpression lysate (Cat# LY400680) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217249 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CDH8 protein (Cat# TP317249). The protein was produced from HEK293T cells transfected with CDH8 cDNA clone (Cat# RC217249) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CDH8
• Synonyms: Nbla04261
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217249 representing NM_001796
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGAACGGCTAGCGGAAATGCTCTTGGATCTCTGGACTCCATTAATAATATTATGGATTACTCTTC
  CCCCTTGCATTTACATGGCTCCGATGAATCAGTCTCAAGTTTTAATGAGTGGATCCCCTTTGGAACTAAA
  CAGTCTGGGTGAAGAACAGCGAATTTTGAACCGCTCCAAAAGAGGCTGGGTTTGGAATCAAATGTTTGTC
  CTGGAAGAGTTTTCTGGACCTGAACCGATTCTTGTTGGCCGGCTACACACAGACCTGGATCCTGGGAGCA
  AAAAAATCAAGTATATCCTATCAGGTGATGGAGCTGGGACCATATTTCAAATAAATGATGTAACTGGAGA
  TATCCATGCTATAAAAAGACTTGACCGGGAGGAAAAGGCTGAGTATACCCTAACAGCTCAAGCAGTGGAC
  TGGGAGACAAGCAAACCTCTGGAGCCTCCTTCTGAATTTATTATTAAAGTTCAAGACATCAATGACAATG
  CACCAGAGTTTCTTAATGGACCCTATCATGCTACTGTGCCAGAAATGTCCATTTTGGGTACATCTGTCAC
  TAACGTCACTGCGACCGACGCTGATGACCCAGTTTATGGAAACAGTGCAAAGTTGGTTTATAGTATATTG
  GAAGGGCAGCCTTATTTTTCCATTGAGCCTGAAACAGCTATTATAAAAACTGCCCTTCCCAACATGGACA
  GAGAAGCCAAGGAGGAGTACCTGGTTGTTATCCAAGCCAAAGATATGGGTGGACACTCTGGTGGCCTGTC
  TGGGACCACGACACTTACAGTGACTCTTACTGATGTTAATGACAATCCTCCAAAATTTGCACAGAGCCTG
  TATCACTTCTCAGTACCGGAAGATGTGGTTCTTGGCACTGCAATAGGAAGGGTGAAGGCCAATGATCAGG
  ATATTGGTGAAAATGCACAGTCATCATATGATATCATCGATGGAGATGGAACAGCACTTTTTGAAATCAC
  TTCTGATGCCCAGGCCCAGGATGGCATTATAAGGCTAAGAAAACCTCTGGACTTTGAGACCAAAAAATCC
  TATACGCTAAAGGTAGAGGCAGCCAATGTCCATATTGACCCACGCTTCAGTGGCAGGGGGCCCTTTAAAG
  ACACGGCGACAGTCAAAATCGTGGTTGAAGATGCTGATGAGCCTCCGGTCTTCTCTTCACCGACTTACCT
  ACTTGAAGTTCATGAAAATGCTGCTCTAAACTCCGTGATTGGGCAAGTGACTGCTCGTGACCCTGATATC
  ACTTCCAGTCCTATAAGGTTTTCCATCGACCGGCACACTGACCTGGAGAGGCAGTTCAACATTAATGCAG
  ACGATGGGAAGATAACGCTGGCAACACCACTTGACAGAGAATTAAGTGTATGGCACAACATAACAATCAT
  TGCTACTGAAATTAGGAACCACAGTCAGATATCACGAGTACCTGTTGCTATTAAAGTGCTGGATGTCAAT
  GACAACGCCCCTGAATTCGCATCCGAATATGAGGCATTTTTATGTGAAAATGGAAAACCCGGCCAAGTCA
  TTCAAACTGTTAGCGCCATGGACAAAGATGATCCCAAAAACGGACATTATTTCTTATACAGTCTCCTTCC
  AGAAATGGTCAACAATCCGAATTTCACCATCAAGAAAAATGAAGATAATTCCCTCAGTATTTTGGCAAAG
  CATAATGGATTCAACCGCCAGAAGCAAGAAGTCTATCTTTTACCAATCATAATCAGTGATAGTGGAAATC
  CTCCACTGAGCAGCACTAGCACCTTGACAATCAGGGTCTGTGGCTGCAGCAATGACGGTGTCGTCCAGTC
  TTGCAATGTCGAAGCTTATGTCCTTCCAATTGGACTCAGTATGGGCGCCTTAATTGCCATATTAGCATGC
  ATCATTTTGCTGTTAGTCATCGTGGTGCTGTTTGTAACTCTACGGCGGCATAAAAATGAACCATTAATTA
  TCAAAGATGATGAAGACGTTCGAGAAAACATCATTCGCTACGATGATGAAGGAGGAGGGGAGGAGGACAC
  AGAGGCTTTTGACATTGCAACTTTACAAAATCCAGATGGAATTAATGGATTTTTACCCCGTAAGGATATT
  AAACCAGATTTGCAGTTTATGCCAAGGCAAGGGCTTGCTCCAGTTCCAAATGGTGTTGATGTCGATGAAT
  TTATAAATGTAAGGCTGCATGAGGCAGATAATGATCCCACGGCCCCGCCATATGACTCCATTCAGATATA
  TGGCTATGAAGGCCGAGGGTCAGTGGCTGGCTCCCTCAGCTCCTTGGAGTCCACCACATCAGACTCAGAC
  CAGAATTTTGACTACCTCAGTGACTGGGGTCCCCGCTTTAAGAGACTGGGCGAACTCTACTCTGTTGGTG
  AAAGTGACAAAGAAACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217249 representing NM_001796
  Red=Cloning site Green=Tags(s)
  
  MPERLAEMLLDLWTPLIILWITLPPCIYMAPMNQSQVLMSGSPLELNSLGEEQRILNRSKRGWVWNQMFV
  LEEFSGPEPILVGRLHTDLDPGSKKIKYILSGDGAGTIFQINDVTGDIHAIKRLDREEKAEYTLTAQAVD
  WETSKPLEPPSEFIIKVQDINDNAPEFLNGPYHATVPEMSILGTSVTNVTATDADDPVYGNSAKLVYSIL
  EGQPYFSIEPETAIIKTALPNMDREAKEEYLVVIQAKDMGGHSGGLSGTTTLTVTLTDVNDNPPKFAQSL
  YHFSVPEDVVLGTAIGRVKANDQDIGENAQSSYDIIDGDGTALFEITSDAQAQDGIIRLRKPLDFETKKS
  YTLKVEAANVHIDPRFSGRGPFKDTATVKIVVEDADEPPVFSSPTYLLEVHENAALNSVIGQVTARDPDI
  TSSPIRFSIDRHTDLERQFNINADDGKITLATPLDRELSVWHNITIIATEIRNHSQISRVPVAIKVLDVN
  DNAPEFASEYEAFLCENGKPGQVIQTVSAMDKDDPKNGHYFLYSLLPEMVNNPNFTIKKNEDNSLSILAK
  HNGFNRQKQEVYLLPIIISDSGNPPLSSTSTLTIRVCGCSNDGVVQSCNVEAYVLPIGLSMGALIAILAC
  IILLLVIVVLFVTLRRHKNEPLIIKDDEDVRENIIRYDDEGGGEEDTEAFDIATLQNPDGINGFLPRKDI
  KPDLQFMPRQGLAPVPNGVDVDEFINVRLHEADNDPTAPPYDSIQIYGYEGRGSVAGSLSSLESTTSDSD
  QNFDYLSDWGPRFKRLGELYSVGESDKET
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001796
• ORF Size: 2397 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001796.2, NM_001796.3, NM_001796.4, NP_001787.2
• RefSeq Size: 2929 bp
• RefSeq ORF: 2400 bp
• Locus ID: 1006
• Cytogenetics: 16q21
• Domains: Cadherin_C_term, CA
• Protein Families: Transmembrane
• MW: 88.25 kDa
• Gene Summary: 'This gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. The extracellular domain consists of 5 subdomains, each containing a cadherin motif, and appears to determine the specificity of the protein's homophilic cell adhesion activity. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. This particular cadherin is expressed in brain and is putatively involved in synaptic adhesion, axon outgrowth and guidance. [provided by RefSeq, Jul 2008]'