Cadherin 8 (CDH8) (NM_001796) Human Untagged Clone

CAT#: SC119022

CDH8 (untagged)-Human cadherin 8, type 2 (CDH8)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDH8
• Synonyms: Nbla04261
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119022 sequence for NM_001796 edited (data generated by NextGen Sequencing)
  ATGCCAGAACGGCTAGCGGAAATGCTCTTGGATCTCTGGACTCCATTAATAATATTATGG
  ATTACTCTTCCCCCTTGCATTTACATGGCTCCGATGAATCAGTCTCAAGTTTTAATGAGT
  GGATCCCCTTTGGAACTAAACAGTCTGGGTGAAGAACAGCGAATTTTGAACCGCTCCAAA
  AGAGGCTGGGTTTGGAATCAAATGTTTGTCCTGGAAGAGTTTTCTGGACCTGAACCGATT
  CTTGTTGGCCGGCTACACACAGACCTGGATCCTGGGAGCAAAAAAATCAAGTATATCCTA
  TCAGGTGATGGAGCTGGGACCATATTTCAAATAAATGATGTAACTGGAGATATCCATGCT
  ATAAAAAGACTTGACCGGGAGGAAAAGGCTGAGTATACCCTAACAGCTCAAGCAGTGGAC
  TGGGAGACAAGCAAACCTCTGGAGCCTCCTTCTGAATTTATTATTAAAGTTCAAGACATC
  AATGACAATGCACCAGAGTTTCTTAATGGACCCTATCATGCTACTGTGCCAGAAATGTCC
  ATTTTGGGTACATCTGTCACTAACGTCACTGCGACCGACGCTGATGACCCAGTTTATGGA
  AACAGTGCAAAGTTGGTTTATAGTATATTGGAAGGGCAGCCTTATTTTTCCATTGAGCCT
  GAAACAGCTATTATAAAAACTGCCCTTCCCAACATGGACAGAGAAGCCAAGGAGGAGTAC
  CTGGTTGTTATCCAAGCCAAAGATATGGGTGGACACTCTGGTGGCCTGTCTGGGACCACG
  ACACTTACAGTGACTCTTACTGATGTTAATGACAATCCTCCAAAATTTGCACAGAGCCTG
  TATCACTTCTCAGTACCGGAAGATGTGGTTCTTGGCACTGCAATAGGAAGGGTGAAGGCC
  AATGATCAGGATATTGGTGAAAATGCACAGTCATCATATGATATCATCGATGGAGATGGA
  ACAGCACTTTTTGAAATCACTTCTGATGCCCAGGCCCAGGATGGCATTATAAGGCTAAGA
  AAACCTCTGGACTTTGAGACCAAAAAATCCTATACGCTAAAGGTAGAGGCAGCCAATGTC
  CATATTGACCCACGCTTCAGTGGCAGGGGGCCCTTTAAAGACACGGCGACAGTCAAAATC
  GTGGTTGAAGATGCTGATGAGCCTCCGGTCTTCTCTTCACCGACTTACCTACTTGAAGTT
  CATGAAAATGCTGCTCTAAACTCCGTGATTGGGCAAGTGACTGCTCGTGACCCTGATATC
  ACTTCCAGTCCTATAAGGTTTTCCATCGACCGGCACACTGACCTGGAGAGGCAGTTCAAC
  ATTAATGCAGACGATGGGAAGATAACGCTGGCAACACCACTTGACAGAGAATTAAGTGTA
  TGGCACAACATAACAATCATTGCTACTGAAATTAGGAACCACAGTCAGATATCACGAGTA
  CCTGTTGCTATTAAAGTGCTGGATGTCAATGACAACGCCCCTGAATTCGCATCCGAATAT
  GAGGCATTTTTATGTGAAAATGGAAAACCCGGCCAAGTCATTCAAACTGTTAGCGCCATG
  GACAAAGATGATCCCAAAAACGGACATTATTTCTTATACAGTCTCCTTCCAGAAATGGTC
  AACAATCCGAATTTCACCATCAAGAAAAATGAAGATAATTCCCTCAGTATTTTGGCAAAG
  CATAATGGATTCAACCGCCAGAAGCAAGAAGTCTATCTTTTACCAATCATAATCAGTGAT
  AGTGGAAATCCTCCACTGAGCAGCACTAGCACCTTGACAATCAGGGTCTGTGGCTGCAGC
  AATGACGGTGTCGTCCAGTCTTGCAATGTCGAAGCTTATGTCCTTCCAATTGGACTCAGT
  ATGGGCGCCTTAATTGCCATATTAGCATGCATCATTTTGCTGTTAGTCATCGTGGTGCTG
  TTTGTAACTCTACGGCGGCATAAAAATGAACCATTAATTATCAAAGATGATGAAGACGTT
  CGAGAAAACATCATTCGCTACGATGATGAAGGAGGAGGGGAGGAGGACACAGAGGCTTTT
  GACATTGCAACTTTACAAAATCCAGATGGAATTAATGGATTTTTACCCCGTAAGGATATT
  AAACCAGATTTGCAGTTTATGCCAAGGCAAGGGCTTGCTCCAGTTCCAAATGGTGTTGAT
  GTCGATGAATTTATAAATGTAAGGCTGCATGAGGCAGATAATGATCCCACGGCCCCGCCA
  TATGACTCCATTCAGATATATGGCTATGAAGGCCGAGGGTCAGTGGCTGGCTCCCTCAGC
  TCCTTGGAGTCCACCACATCAGACTCAGACCAGAATTTTGACTACCTCAGTGACTGGGGT
  CCCCGCTTTAAGAGACTGGGCGAACTCTACTCTGTTGGTGAAAGTGACAAAGAAACTTGA
  
  Clone variation with respect to NM_001796.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001796 unedited
  AAGCTTTGGATTTGTAATCCNACTTACTATAGNNGGCGGCCGCGCAATTCGCACGAGGGC
  CGCCGCCGCCGGCTTGTGCATAGACGGTAGCGTCAAGACACCAGCTGCACTTTGGGTTGC
  GGACACCCAGAGCCCAGCGCGCCCGCCCTGCGTCTCCGCACCAAGCCCTGTAGCTCCGAG
  AGGCATGAACGGAATCCGGAGGCGCCTGCCTAGCGAGCGAGGACCGGTCGGCGCTTGCCG
  CCCTCGGGAGCTAACCCCGAGCACCTCCAGCCATTTGTGAACCTGGAGGCTTGACATTCG
  CCAGCGCAGGGCCCCACAAGAGAAATTTCAATGAAAAGAAAAGCCAATGGATTGTGGTCT
  TAGAAAAGCTGCTTAGATGATGTCTGTTTCCCGTGCTATAGACACGTGGCAGAGCTGTAA
  GTAAATGCTCGGCACTGCATGATGAATTGGATGGCTGCAGACCGGAGACAAAAAAAATAA
  TTGTCTCATTTTCGTGGTGATTTGCTTAACTGGTGGGACCATGCCAGAACGGCTAGCGGA
  AATGCTCTTGGATCTCTGGACTCCATTAATAATATTATGGATTACTCTTCCCCCTTGCAT
  TTACATGGCTCCGATGAATCAGTCTCAAGTTTTAATGAGTGGATCCNCTTTGGAACTAAA
  CAGTCTGGGTGAAGAACAGCGAATTTTGAACCGCTCCAAAAGAGGCTGGGTTTGGAATCA
  AATGTTTGTCCTGGAAGAGTTTCTGGACCTGAACCGATTCTTGTTGGCCGGCTACACACA
  GACCTGGATCCTGNGAGCANAAAAATCAAGTATATCCTATCAGGTGATGGAGCTGGGACC
  ATATTTCAATNNAATGATGTACTGGAGATATCCATGCTATAAAAAGACTTGACCGGGGAG
  AAAAAGCTGAGTATACCCNNTACAGCTCAGCAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001796 unedited
  GCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGTTCAACATTAAAATG
  TGGTTGAGTTTATAGATGACTGGTGCTAAACTTGCCTCTAAAACAAATAGCCACATTGGT
  TGTATCTAAGGGGAGTGACCCTAGAATATTACAGAATGCTCAGTTCCAGTGATTTATTTA
  TAATCCACTGTCAAGTTTCTTTGTCACTTTCACCAACAGAGTAGAGTTCGCCCAGTCTCT
  TAAAGCGGGGACCCCAGTCACTGAGGTAGTCAAAATTCTGGTCTGAGTCTGATGTGGTGG
  ACTCCAAGGAGCTGAGGGAGCCAGCCACTGACCCTCGGCCTTCATAGCCATATATCTGAA
  TGGAGTCATATGGCGGGGCCGTGGGATCATTATCTGCCTCATGCAGCCTTACATTTATAA
  ATTCATCGACATCAACACCATTTGGAACTGGAGCAAGCCCTTGCCTTGGCATAAACTGCA
  AATCTGGTTTAATATCCTTACGGGGTAAAAATCCATTAATTCCATCTGGATTTTGTAAAG
  TTGCAATGTCAAAAGCCTCTGTGTCCTCCTCCCCTCCTCCTTCATCATCGTAGCGAATGA
  TGTTTTCTCGAACGTCTTCATCATCTTTGATAATTAATGGTTCATTTTTATGCCGCCGTA
  GAGTTACAAACAGCACCACGATGACTAACAGCAAAATGATGCATGCTAATATGGCAATTA
  AGGCGCCCATACTGAGTNCAATTGAAGGACATNNAGCTCGACATTGCAGACTGGACGACA
  CCGTCATTGCTGCAGCCACAGACCCTGATGTCAAGGGGCTAGTGCTGCTCATGGNAGATT
  TTCCCTTCACTGATATGGATGGTAAAGATAACTTCTGCTNCTGGCGNTGAATCATTATGC
  TTTGCCAATACTGAGGAATATCTTCATTTCCTTGTGTGGATTCCGATGTTGACATTCTGG
  ANGAGATGNTANAAAAAAGGCCGTTTGGGAACACCTTGCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001796
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001796.2, NP_001787.2
• RefSeq Size: 2929 bp
• RefSeq ORF: 2400 bp
• Locus ID: 1006
• Cytogenetics: 16q21
• Domains: Cadherin_C_term, CA
• Protein Families: Transmembrane
• Gene Summary: 'This gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. The extracellular domain consists of 5 subdomains, each containing a cadherin motif, and appears to determine the specificity of the protein's homophilic cell adhesion activity. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. This particular cadherin is expressed in brain and is putatively involved in synaptic adhesion, axon outgrowth and guidance. [provided by RefSeq, Jul 2008]'