DNA2 (NM_001080449) Human Tagged ORF Clone

CAT#: RC217395

DNA2 (Myc-DDK-tagged)-Human DNA replication helicase 2 homolog (yeast) (DNA2)

Product Images

Western blot validation of overexpression lysate (Cat# LY420707) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217395 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DNA2
• Synonyms: DNA2L; hDNA2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217395 representing NM_001080449
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGACTCCCTGTATTCCCAGTCCTAAGCAAGGGAGCAAAGGCAGGGGACAGAGCCCTGCTGCTCAGG
  TGAGGGGCCCCACGTGGAACGCGCCGGCGCGGGAGGGGCGGCCTGGCGCAGGTCATTTGGGACATCTGCG
  GGTTGGCGCATGCGCGCGAGGTGCGCAGGCTCGCGCCTTTTTCCCTTTTCTAAGCTTTCTGTGTTACCCC
  CGGTTCCGCTGTCTTTTCTGTCTACAGTTTGCGATCCCCGCGTCCAGGATGGAGCAGCTGAACGAACTGG
  AGCTGCTGATGGAGAAGAGTTTTTGGGAGGAGGCGGAGCTGCCGGCGGAGCTATTTCAGAAGAAAGTGGT
  AGCTTCCTTTCCAAGAACAGTTCTGAGCACAGGAATGGATAACCGGTACCTGGTGTTGGCAGTCAATACT
  GTACAGAACAAAGAGGGAAACTGTGAAAAGCGCCTGGTCATCACTGCTTCACAGTCACTAGAAAATAAAG
  AACTATGCATCCTTAGGAATGACTGGTGTTCTGTTCCAGTAGAGCCAGGAGATATCATTCATTTGGAGGG
  AGACTGCACATCTGACACTTGGATAATAGATAAAGATTTTGGATATTTGATTCTGTATCCAGACATGCTG
  ATTTCTGGCACCAGCATAGCCAGTAGTATTCGATGTATGAGAAGAGCTGTCCTGAGTGAAACTTTTAGGA
  GCTCTGATCCAGCCACACGCCAAATGCTAATTGGTACGGTTCTCCATGAGGTGTTTCAAAAAGCCATAAA
  TAATAGCTTTGCCCCAGAAAAGCTACAAGAACTTGCTTTTCAAACAATTCAAGAAATAAGACATTTGAAG
  GAAATGTACCGCTTAAATCTAAGTCAAGATGAAATAAAACAAGAAGTAGAGGACTATCTTCCTTCGTTTT
  GTAAATGGGCAGGAGATTTCATGCATAAAAACACTTCGACTGACTTCCCTCAGATGCAGCTCTCTCTGCC
  AAGTGATAATAGTAAGGATAATTCAACATGTAACATTGAAGTCGTGAAACCAATGGATATTGAAGAAAGC
  ATTTGGTCCCCTAGGTTTGGATTGAAAGGCAAAATAGATGTTACAGTTGGTGTGAAAATACATCGAGGGT
  ATAAAACAAAATACAAGATAATGCCGCTGGAACTTAAAACTGGCAAAGAATCAAATTCTATTGAACACCG
  TAGTCAGGTTGTTCTGTACACTCTACTAAGCCAAGAGAGAAGAGCTGATCCAGAGGCTGGCTTGCTTCTC
  TACCTCAAGACTGGTCAGATGTACCCTGTGCCTGCCAACCATCTAGATAAAAGAGAATTATTAAAGCTAA
  GAAACCAGATGGCATTCTCATTGTTTCACCGTATTAGCAAATCTGCTACTAGACAGAAGACACAGCTTGC
  TTCTTTGCCACAAATAATTGAGGAAGAGAAAACTTGTAAATATTGTTCACAAATTGGCAATTGTGCTCTT
  TATAGCAGAGCAGTTGAACAACAGATGGATTGTAGTTCAGTCCCAATTGTGATGCTGCCCAAAATAGAAG
  AAGAAACCCAGCATCTGAAGCAAACACACTTAGAATATTTCAGCCTTTGGTGTCTAATGTTAACCCTGGA
  GTCACAATCGAAGGATAATAAAAAGAATCACCAAAATATCTGGCTAATGCCTGCTTCGGAAATGGAGAAG
  AGTGGCAGTTGCATTGGAAACCTGATTAGAATGGAACATGTAAAGATAGTTTGTGATGGGCAATATTTAC
  ATAATTTCCAATGTAAACATGGTGCCATACCTGTCACAAATCTAATGGCAGGTGACAGAGTTATTGTAAG
  TGGAGAAGAAAGGTCACTGTTTGCTTTGTCTAGAGGATATGTGAAGGAGATTAACATGACAACAGTAACT
  TGTTTATTAGACAGAAACTTGTCGGTCCTTCCAGAATCAACTTTGTTCAGATTAGACCAAGAAGAAAAAA
  ATTGTGATATAGATACCCCATTAGGAAATCTTTCCAAATTGATGGAAAACACGTTTGTCAGCAAAAAACT
  TCGAGATTTAATTATTGACTTTCGTGAACCTCAGTTTATATCCTACCTTAGTTCTGTTCTTCCACATGAT
  GCAAAGGATACAGTTGCCTGCATTCTAAAGGGTTTGAATAAGCCTCAGAGGCAAGCGATGAAAAAGGTAC
  TTCTTTCAAAAGACTACACACTCATCGTGGGTATGCCTGGGACAGGAAAAACAACTACGATATGTACTCT
  CGTAAGAATTCTCTACGCCTGTGGTTTTAGCGTTTTGTTGACCAGCTATACACACTCTGCTGTTGACAAT
  ATTCTTTTGAAGTTAGCCAAGTTTAAAATAGGATTTTTGCGTTTGGGTCAGATTCAGAAGGTTCATCCAG
  CTATCCAGCAATTTACAGAGCAAGAAATTTGCAGATCAAAGTCCATTAAATCCTTAGCTCTTCTAGAAGA
  ACTCTACAATAGTCAACTTATAGTTGCAACAACATGTATGGGAATAAACCATCCAATATTTTCCCGTAAA
  ATTTTTGATTTTTGTATTGTGGATGAAGCCTCTCAAATTAGCCAACCAATTTGTCTGGGCCCCCTTTTTT
  TTTCACGGAGATTTGTGTTAGTGGGGGACCATCAGCAGCTTCCTCCCCTGGTGCTAAACCGTGAAGCAAG
  AGCTCTTGGCATGAGTGAAAGCTTATTCAAGAGGCTGGAGCAGAATAAGAGTGCTGTTGTACAGTTAACC
  GTGCAGTACAGAATGAACAGTAAAATTATGTCCTTAAGTAATAAGCTGACCTATGAGGGCAAGCTGGAGT
  GTGGATCAGACAAAGTGGCCAATGCAGTGATAAACCTACGTCACTTTAAAGATGTGAAGCTGGAACTGGA
  ATTTTATGCTGACTATTCTGATAATCCTTGGTTGATGGGAGTATTTGAACCCAACAATCCTGTTTGTTTC
  CTTAATACAGACAAGGTTCCAGCGCCAGAACAAGTTGAAAAAGGTGGTGTGAGCAATGTAACAGAAGCCA
  AACTCATAGTTTTCCTAACCTCCATTTTTGTTAAGGCTGGATGCAGTCCCTCTGATATTGGTATTATTGC
  ACCGTACAGGCAGCAATTAAAGATCATCAATGATTTATTGGCACGTTCTATTGGGATGGTCGAAGTTAAT
  ACAGTAGACAAATACCAAGGAAGGGACAAAAGTATTGTCCTAGTATCTTTTGTTAGAAGTAATAAGGATG
  GAACTGTTGGTGAACTCTTGAAAGATTGGCGACGTCTTAATGTTGCTATAACCAGAGCCAAACATAAACT
  GATTCTTCTGGGGTGTGTGCCCTCACTAAATTGCTATCCTCCTTTGGAGAAGCTGCTTAATCATTTAAAC
  TCAGAAAAATTAATCATTGATCTTCCATCAAGAGAACATGAAAGTCTTTGCCACATATTGGGTGACTTTC
  AAAGAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217395 representing NM_001080449
  Red=Cloning site Green=Tags(s)
  
  MKTPCIPSPKQGSKGRGQSPAAQVRGPTWNAPAREGRPGAGHLGHLRVGACARGAQARAFFPFLSFLCYP
  RFRCLFCLQFAIPASRMEQLNELELLMEKSFWEEAELPAELFQKKVVASFPRTVLSTGMDNRYLVLAVNT
  VQNKEGNCEKRLVITASQSLENKELCILRNDWCSVPVEPGDIIHLEGDCTSDTWIIDKDFGYLILYPDML
  ISGTSIASSIRCMRRAVLSETFRSSDPATRQMLIGTVLHEVFQKAINNSFAPEKLQELAFQTIQEIRHLK
  EMYRLNLSQDEIKQEVEDYLPSFCKWAGDFMHKNTSTDFPQMQLSLPSDNSKDNSTCNIEVVKPMDIEES
  IWSPRFGLKGKIDVTVGVKIHRGYKTKYKIMPLELKTGKESNSIEHRSQVVLYTLLSQERRADPEAGLLL
  YLKTGQMYPVPANHLDKRELLKLRNQMAFSLFHRISKSATRQKTQLASLPQIIEEEKTCKYCSQIGNCAL
  YSRAVEQQMDCSSVPIVMLPKIEEETQHLKQTHLEYFSLWCLMLTLESQSKDNKKNHQNIWLMPASEMEK
  SGSCIGNLIRMEHVKIVCDGQYLHNFQCKHGAIPVTNLMAGDRVIVSGEERSLFALSRGYVKEINMTTVT
  CLLDRNLSVLPESTLFRLDQEEKNCDIDTPLGNLSKLMENTFVSKKLRDLIIDFREPQFISYLSSVLPHD
  AKDTVACILKGLNKPQRQAMKKVLLSKDYTLIVGMPGTGKTTTICTLVRILYACGFSVLLTSYTHSAVDN
  ILLKLAKFKIGFLRLGQIQKVHPAIQQFTEQEICRSKSIKSLALLEELYNSQLIVATTCMGINHPIFSRK
  IFDFCIVDEASQISQPICLGPLFFSRRFVLVGDHQQLPPLVLNREARALGMSESLFKRLEQNKSAVVQLT
  VQYRMNSKIMSLSNKLTYEGKLECGSDKVANAVINLRHFKDVKLELEFYADYSDNPWLMGVFEPNNPVCF
  LNTDKVPAPEQVEKGGVSNVTEAKLIVFLTSIFVKAGCSPSDIGIIAPYRQQLKIINDLLARSIGMVEVN
  TVDKYQGRDKSIVLVSFVRSNKDGTVGELLKDWRRLNVAITRAKHKLILLGCVPSLNCYPPLEKLLNHLN
  SEKLIIDLPSREHESLCHILGDFQRE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001080449
• ORF Size: 3438 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001080449.1, NP_001073918.1
• RefSeq Size: 4416 bp
• RefSeq ORF: 3183 bp
• Locus ID: 1763
• Cytogenetics: 10q21.3
• Protein Pathways: DNA replication
• MW: 129.5 kDa
• Gene Summary: 'This gene encodes a member of the DNA2/NAM7 helicase family. The encoded protein is a conserved helicase/nuclease involved in the maintenance of mitochondrial and nuclear DNA stability. Mutations in this gene are associated with autosomal dominant progressive external ophthalmoplegia-6 (PEOA6) and Seckel syndrome 8. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2014]'