DNA2 (NM_001080449) Human Untagged Clone

CAT#: SC315872

DNA2 (untagged)-Human DNA replication helicase 2 homolog (yeast) (DNA2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DNA2
• Synonyms: DNA2L; hDNA2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001080449 edited
  ATGAAGACTCCCTGTATTCCCAGTCCTAAGCAAGGGAGCAAAGGCAGGGGACAGAGCCCT
  GCTGCTCAGGTGAGGGGCCCCACGTGGAACGCGCCGGCGCGGGAGGGGCGGCCTGGCGCA
  GGTCATTTGGGACATCTGCGGGTTGGCGCATGCGCGCGAGGTGCGCAGGCTCGCGCCTTT
  TTCCCTTTTCTAAGCTTTCTGTGTTACCCCCGGTTCCGCTGTCTTTTCTGTCTACAGTTT
  GCGATCCCCGCGTCCAGGATGGAGCAGCTGAACGAACTGGAGCTGCTGATGGAGAAGAGT
  TTTTGGGAGGAGGCGGAGCTGCCGGCGGAGCTATTTCAGAAGAAAGTGGTAGCTTCCTTT
  CCAAGAACAGTTCTGAGCACAGGAATGGATAACCGGTACCTGGTGTTGGCAGTCAATACT
  GTACAGAACAAAGAGGGAAACTGTGAAAAGCGCCTGGTCATCACTGCTTCACAGTCACTA
  GAAAATAAAGAACTATGCATCCTTAGGAATGACTGGTGTTCTGTTCCAGTAGAGCCAGGA
  GATATCATTCATTTGGAGGGAGACTGCACATCTGACACTTGGATAATAGATAAAGATTTT
  GGATATTTGATTCTGTATCCAGACATGCTGATTTCTGGCACCAGCATAGCCAGTAGTATT
  CGATGTATGAGAAGAGCTGTCCTGAGTGAAACTTTTAGGAGCTCTGATCCAGCCACACGC
  CAAATGCTAATTGGTACGGTTCTCCATGAGGTGTTTCAAAAAGCCATAAATAATAGCTTT
  GCCCCAGAAAAGCTACAAGAACTTGCTTTTCAAACAATTCAAGAAATAAGACATTTGAAG
  GAAATGTACCGCTTAAATCTAAGTCAAGATGAAATAAAACAAGAAGTAGAGGACTATCTT
  CCTTCGTTTTGTAAATGGGCAGGAGATTTCATGCATAAAAACACTTCGACTGACTTCCCT
  CAGATGCAGCTCTCTCTGCCAAGTGATAATAGTAAGGATAATTCAACATGTAACATTGAA
  GTCGTGAAACCAATGGATATTGAAGAAAGCATTTGGTCCCCTAGGTTTGGATTGAAAGGC
  AAAATAGATGTTACAGTTGGTGTGAAAATACATCGAGGGTATAAAACAAAATACAAGATA
  ATGCCGCTGGAACTTAAAACTGGCAAAGAATCAAATTCTATTGAACACCGTAGTCAGGTT
  GTTCTGTACACTCTACTAAGCCAAGAGAGAAGAGCTGATCCAGAGGCTGGCTTGCTTCTC
  TACCTCAAGACTGGTCAGATGTACCCTGTGCCTGCCAACCATCTAGATAAAAGAGAATTA
  TTAAAGCTAAGAAACCAGATGGCATTCTCATTGTTTCACCGTATTAGCAAATCTGCTACT
  AGACAGAAGACACAGCTTGCTTCTTTGCCACAAATAATTGAGGAAGAGAAAACTTGTAAA
  TATTGTTCACAAATTGGCAATTGTGCTCTTTATAGCAGAGCAGTTGAACAACAGATGGAT
  TGTAGTTCAGTCCCAATTGTGATGCTGCCCAAAATAGAAGAAGAAACCCAGCATCTGAAG
  CAAACACACTTAGAATATTTCAGCCTTTGGTGTCTAATGTTAACCCTGGAGTCACAATCG
  AAGGATAATAAAAAGAATCACCAAAATATCTGGCTAATGCCTGCTTCGGAAATGGAGAAG
  AGTGGCAGTTGCATTGGAAACCTGATTAGAATGGAACATGTAAAGATAGTTTGTGATGGG
  CAATATTTACATAATTTCCAATGTAAACATGGTGCCATACCTGTCACAAATCTAATGGCA
  GGTGACAGAGTTATTGTAAGTGGAGAAGAAAGGTCACTGTTTGCTTTGTCTAGAGGATAT
  GTGAAGGAGATTAACATGACAACAGTAACTTGTTTATTAGACAGAAACTTGTCGGTCCTT
  CCAGAATCAACTTTGTTCAGATTAGACCAAGAAGAAAAAAATTGTGATATAGATACCCCA
  TTAGGAAATCTTTCCAAATTGATGGAAAACACGTTTGTCAGCAAAAAACTTCGAGATTTA
  ATTATTGACTTTCGTGAACCTCAGTTTATATCCTACCTTAGTTCTGTTCTTCCACATGAT
  GCAAAGGATACAGTTGCCTGCATTCTAAAGGGTTTGAATAAGCCTCAGAGGCAAGCGATG
  AAAAAGGTACTTCTTTCAAAAGACTACACACTCATCGTGGGTATGCCTGGGACAGGAAAA
  ACAACTACGATATGTACTCTCGTAAGAATTCTCTACGCCTGTGGTTTTAGCGTTTTGTTG
  ACCAGCTATACACACTCTGCTGTTGACAATATTCTTTTGAAGTTAGCCAAGTTTAAAATA
  GGATTTTTGCGTTTGGGTCAGATTCAGAAGGTTCATCCAGCTATCCAGCAATTTACAGAG
  CAAGAAATTTGCAGATCAAAGTCCATTAAATCCTTAGCTCTTCTAGAAGAACTCTACAAT
  AGTCAACTTATAGTTGCAACAACATGTATGGGAATAAACCATCCAATATTTTCCCGTAAA
  ATTTTTGATTTTTGTATTGTGGATGAAGCCTCTCAAATTAGCCAACCAATTTGTCTGGGC
  CCCCTTTTTTTTTCACGGAGATTTGTGTTAGTGGGGGACCATCAGCAGCTTCCTCCCCTG
  GTGCTAAACCGTGAAGCAAGAGCTCTTGGCATGAGTGAAAGCTTATTCAAGAGGCTGGAG
  CAGAATAAGAGTGCTGTTGTACAGTTAACCGTGCAGTACAGAATGAACAGTAAAATTATG
  TCCTTAAGTAATAAGCTGACCTATGAGGGCAAGCTGGAGTGTGGATCAGACAAAGTGGCC
  AATGCAGTGATAAACCTACGTCACTTTAAAGATGTGAAGCTGGAACTGGAATTTTATGCT
  GACTATTCTGATAATCCTTGGTTGATGGGAGTATTTGAACCCAACAATCCTGTTTGTTTC
  CTTAATACAGACAAGGTTCCAGCGCCAGAACAAGTTGAAAAAGGTGGTGTGAGCAATGTA
  ACAGAAGCCAAACTCATAGTTTTCCTAACCTCCATTTTTGTTAAGGCTGGATGCAGTCCC
  TCTGATATTGGTATTATTGCACCGTACAGGCAGCAATTAAAGATCATCAATGATTTATTG
  GCACGTTCTATTGGGATGGTCGAAGTTAATACAGTAGACAAATACCAAGGAAGGGACAAA
  AGTATTGTCCTAGTATCTTTTGTTAGAAGTAATAAGGATGGAACTGTTGGTGAACTCTTG
  AAAGATTGGCGACGTCTTAATGTTGCTATAACCAGAGCCAAACATAAACTGATTCTTCTG
  GGGTGTGTGCCCTCACTAAATTGCTATCCTCCTTTGGAGAAGCTGCTTAATCATTTAAAC
  TCAGAAAAATTAATCATTGATCTTCCATCAAGAGAACATGAAAGTCTTTGCCACATATTG
  GGTGACTTTCAAAGAGAATAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001080449
• Insert Size: 4500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: ORF was fully sequenced and the sequence matches to NM_001080449.1.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001080449.1, NP_001073918.1
• RefSeq Size: 4416 bp
• RefSeq ORF: 3441 bp
• Locus ID: 1763
• Cytogenetics: 10q21.3
• Protein Pathways: DNA replication
• Gene Summary: 'This gene encodes a member of the DNA2/NAM7 helicase family. The encoded protein is a conserved helicase/nuclease involved in the maintenance of mitochondrial and nuclear DNA stability. Mutations in this gene are associated with autosomal dominant progressive external ophthalmoplegia-6 (PEOA6) and Seckel syndrome 8. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2014]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein.