DROSHA (NM_001100412) Human Tagged ORF Clone

CAT#: RC217782

DROSHA (Myc-DDK-tagged)-Human drosha, ribonuclease type III (DROSHA), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DROSHA
• Synonyms: ETOHI2; HSA242976; RANSE3L; RN3; RNASE3L; RNASEN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217782 representing NM_001100412
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGCAGGGAAACACATGTCACAGAATGTCGTTCCACCCGGGACGAGGGTGTCCCCGAGGACGAGGAG
  GACATGGAGCCAGACCCTCAGCACCATCCTTTAGGCCCCAAAATCTGAGGCTGCTTCACCCTCAGCAGCC
  TCCTGTGCAATATCAATATGAACCTCCAAGTGCCCCTTCCACCACTTTCTCAAACTCTCCAGCCCCCAAT
  TTTCTCCCTCCACGACCAGACTTTGTACCCTTCCCCCCACCCATGCCTCCGTCAGCGCAAGGCCCTCTTC
  CCCCCTGCCCAATCAGGCCGCCTTTCCCCAACCACCAGATGAGGCACCCCTTCCCAGTTCCTCCTTGTTT
  TCCTCCCATGCCACCACCAATGCCTTGTCCTAATAACCCCCCAGTCCCTGGGGCACCTCCTGGACAAGGC
  ACTTTCCCCTTCATGATGCCCCCTCCCTCCATGCCTCATCCCCCGCCCCCTCCAGTCATGCCGCAGCAGG
  TTAATTATCAGTACCCTCCGGGCTATTCTCACCACAACTTCCCACCTCCCAGTTTTAATAGTTTCCAGAA
  CAACCCTAGTTCTTTCCTGCCCAGTGCTAATAACAGCAGTAGTCCTCATTTCAGACATCTCCCTCCATAC
  CCACTCCCAAAGGCTCCCAGTGAGAGAAGGTCCCCAGAAAGGCTGAAACACTATGATGACCACAGGCACC
  GAGATCACAGTCATGGGCGAGGTGAGAGGCATCGGTCCCTGGATCGGCGGGAGCGAGGCCGCAGTCCCGA
  CAGGAGAAGACAAGACAGCCGGTACAGATCTGATTATGACCGAGGGAGAACACCATCTCGCCACCGCAGC
  TACGAACGGAGCAGAGAGCGAGAACGGGAGAGACACAGGCATCGAGACAACCGAAGATCACCATCTCTGG
  AAAGGTCCTACAAAAAAGAGTATAAGAGATCTGGAAGTCGCTCCCCAAGTAGGGAGAAGAAGAGAGCTCG
  TTGGGAGGAAGAAAAAGACCGTTGGAGTGACAACCAGAGTTCTGGCAAAGACAAGAACTATACCTCAATC
  AAGGAAAAAGAGCCCGAGGAGACCATGCCTGACAAGAATGAGGAGGAAGAAGAAGAACTTCTTAAGCCTG
  TGTGGATTCGATGCACTCATTCAGAAAACTACTACTCCAGTGACCCCATGGATCAGGTGGGAGATTCTAC
  AGTGGTTGGAACGAGTAGGCTTCGTGACTTATATGACAAATTTGAGGAGGAGTTGGGGAGCAGGCAAGAA
  AAGGCCAAAGCTGCTCGGCCTCCGTGGGAACCTCCAAAGACGAAGCTCGATGAAGATTTAGAGAGTTCCA
  GTGAATCCGAGTGTGAGTCTGATGAGGACAGCACCTGTTCTAGCAGCTCAGACTCTGAAGTTTTTGACGT
  TATTGCAGAAATCAAACGCAAAAAGGCCCACCCTGACCGACTTCATGATGAACTTTGGTACAACGATCCA
  GGCCAGATGAATGATGGACCACTCTGCAAATGCAGCGCAAAGGCAAGACGCACAGGAATTAGGCACAGCA
  TTTATCCTGGAGAAGAGGCCATCAAGCCCTGTCGTCCTATGACCAACAATGCTGGCAGACTTTTCCACTA
  CCGGATCACAGTCTCCCCGCCTACGAACTTTTTAACTGACAGGCCAACTGTTATAGAATACGATGATCAC
  GAGTATATCTTTGAAGGATTTTCTATGTTTGCACATGCCCCCCTGACCAATATTCCACTGTGTAAAGTAA
  TTAGATTCAACATAGACTACACGATTCATTTCATTGAAGAGATGATGCCGGAGAATTTTTGTGTGAAAGG
  GCTTGAACTCTTTTCACTGTTCCTATTCAGAGATATTTTGGAATTATATGACTGGAATCTTAAAGGTCCT
  TTGTTTGAAGACAGCCCTCCCTGCTGCCCAAGATTTCATTTCATGCCACGTTTTGTAAGATTTCTTCCAG
  ATGGAGGAAAGGAAGTGCTGTCCATGCACCAGATTCTCCTGTACTTGTTAAGGTGCAGCAAAGCCCTGGT
  GCCTGAGGAGGAGATTGCCAATATGCTTCAGTGGGAGGAGCTGGAGTGGCAGAAATATGCAGAAGAATGC
  AAAGGCATGATTGTTACCAACCCTGGGACGAAACCAAGCTCTGTCCGTATCGATCAACTGGATCGTGAAC
  AGTTCAACCCCGATGTGATTACTTTTCCGATTATCGTCCACTTTGGGATACGCCCTGCACAGTTGAGTTA
  TGCAGGAGACCCACAGTACCAAAAACTGTGGAAGAGTTATGTGAAACTTCGCCACCTCCTAGCAAATAGT
  CCCAAAGTCAAACAAACTGACAAACAGAAGCTGGCACAGAGGGAGGAAGCCCTCCAAAAAATACGGCAGA
  AGAATACAATGAGACGAGAAGTAACGGTGGAGCTAAGTAGCCAAGGATTCTGGAAAACTGGCATCCGTTC
  TGATGTCTGTCAGCATGCAATGATGCTACCTGTTCTGACCCATCATATCCGCTACCACCAATGCCTAATG
  CATTTGGACAAGTTGATAGGATATACTTTCCAAGATCGTTGTCTGTTGCAGCTGGCCATGACTCATCCAA
  GTCATCATTTAAATTTTGGAATGAATCCTGATCATGCCAGGAATTCATTATCTAACTGTGGAATTCGGCA
  GCCCAAATACGGAGACAGAAAAGTTCATCACATGCACATGCGGAAGAAAGGGATTAACACCTTGATAAAT
  ATCATGTCACGCCTTGGCCAAGATGACCCAACTCCCTCGAGGATTAACCACAATGAACGGTTGGAATTCC
  TGGGTGATGCTGTTGTTGAATTTCTGACCAGCGTCCATTTGTACTATTTGTTTCCTAGTCTGGAAGAAGG
  AGGATTAGCAACCTATCGGACTGCCATTGTTCAGAATCAGCACCTTGCCATGCTAGCAAAGAAACTTGAA
  CTGGATCGATTTATGCTGTATGCTCACGGGCCTGACCTTTGTAGAGAATCGGACCTTCGACATGCAATGG
  CCAATTGTTTTGAAGCGTTAATAGGAGCTGTTTACTTGGAGGGAAGCCTGGAGGAAGCCAAGCAGTTATT
  TGGACGCTTGCTCTTTAATGATCCGGACCTGCGCGAAGTCTGGCTCAATTATCCTCTCCACCCACTCCAA
  CTACAAGAGCCAAATACTGATCGACAACTTATTGAAACTTCTCCAGTTCTACAAAAACTTACTGAGTTTG
  AAGAAGCAATTGGAGTAATTTTTACTCATGTTCGACTTCTGGCAAGGGCATTCACATTGAGAACTGTGGG
  ATTTAACCATCTGACCCTAGGCCACAATCAGAGAATGGAATTCCTAGGTGACTCCATAATGCAACTGGTA
  GCCACAGAGTACTTATTCATTCATTTCCCAGATCATCATGAAGGACACTTAACTTTGTTGCGAAGCTCTT
  TGGTGAATAATAGAACTCAGGCCAAGGTAGCGGAGGAGCTGGGCATGCAGGAGTACGCCATAACCAACGA
  CAAGACCAAGAGGCCTGTGGCGCTTCGCACCAAGACCTTGGCGGACCTTTTGGAATCATTTATTGCAGCG
  CTGTACATTGATAAGGATTTGGAATATGTTCATACTTTCATGAATGTCTGCTTCTTTCCACGATTGAAAG
  AGTTCATTTTGAATCAGGATTGGAATGACCCCAAATCCCAGCTTCAGCAGTGTTGCTTGACACTTAGGAC
  AGAAGGAAAAGAGCCAGACATTCCTCTGTACAAGACTCTGCAGACAGTGGGCCCATCCCATGCCCGAACC
  TACACTGTGGCTGTTTATTTCAAGGGAGAAAGAATAGGCTGTGGGAAAGGACCAAGTATTCAGCAAGCGG
  AAATGGGAGCAGCAATGGATGCGCTTGAAAAATATAATTTTCCCCAGATGGCCCATCAGAAGCGGTTCAT
  CGAACGGAAGTACAGACAAGAGTTAAAAGAAATGAGGTGGGAAAGAGAGCATCAAGAGAGAGAGCCAGAT
  GAGACTGAAGACATCAAGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217782 representing NM_001100412
  Red=Cloning site Green=Tags(s)
  
  MMQGNTCHRMSFHPGRGCPRGRGGHGARPSAPSFRPQNLRLLHPQQPPVQYQYEPPSAPSTTFSNSPAPN
  FLPPRPDFVPFPPPMPPSAQGPLPPCPIRPPFPNHQMRHPFPVPPCFPPMPPPMPCPNNPPVPGAPPGQG
  TFPFMMPPPSMPHPPPPPVMPQQVNYQYPPGYSHHNFPPPSFNSFQNNPSSFLPSANNSSSPHFRHLPPY
  PLPKAPSERRSPERLKHYDDHRHRDHSHGRGERHRSLDRRERGRSPDRRRQDSRYRSDYDRGRTPSRHRS
  YERSRERERERHRHRDNRRSPSLERSYKKEYKRSGSRSPSREKKRARWEEEKDRWSDNQSSGKDKNYTSI
  KEKEPEETMPDKNEEEEEELLKPVWIRCTHSENYYSSDPMDQVGDSTVVGTSRLRDLYDKFEEELGSRQE
  KAKAARPPWEPPKTKLDEDLESSSESECESDEDSTCSSSSDSEVFDVIAEIKRKKAHPDRLHDELWYNDP
  GQMNDGPLCKCSAKARRTGIRHSIYPGEEAIKPCRPMTNNAGRLFHYRITVSPPTNFLTDRPTVIEYDDH
  EYIFEGFSMFAHAPLTNIPLCKVIRFNIDYTIHFIEEMMPENFCVKGLELFSLFLFRDILELYDWNLKGP
  LFEDSPPCCPRFHFMPRFVRFLPDGGKEVLSMHQILLYLLRCSKALVPEEEIANMLQWEELEWQKYAEEC
  KGMIVTNPGTKPSSVRIDQLDREQFNPDVITFPIIVHFGIRPAQLSYAGDPQYQKLWKSYVKLRHLLANS
  PKVKQTDKQKLAQREEALQKIRQKNTMRREVTVELSSQGFWKTGIRSDVCQHAMMLPVLTHHIRYHQCLM
  HLDKLIGYTFQDRCLLQLAMTHPSHHLNFGMNPDHARNSLSNCGIRQPKYGDRKVHHMHMRKKGINTLIN
  IMSRLGQDDPTPSRINHNERLEFLGDAVVEFLTSVHLYYLFPSLEEGGLATYRTAIVQNQHLAMLAKKLE
  LDRFMLYAHGPDLCRESDLRHAMANCFEALIGAVYLEGSLEEAKQLFGRLLFNDPDLREVWLNYPLHPLQ
  LQEPNTDRQLIETSPVLQKLTEFEEAIGVIFTHVRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLV
  ATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVAEELGMQEYAITNDKTKRPVALRTKTLADLLESFIAA
  LYIDKDLEYVHTFMNVCFFPRLKEFILNQDWNDPKSQLQQCCLTLRTEGKEPDIPLYKTLQTVGPSHART
  YTVAVYFKGERIGCGKGPSIQQAEMGAAMDALEKYNFPQMAHQKRFIERKYRQELKEMRWEREHQEREPD
  ETEDIKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001100412
• ORF Size: 4011 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001100412.1, NP_001093882.1
• RefSeq Size: 5389
• RefSeq ORF: 4014
• Locus ID: 29102
• MW: 155.2 kDa
• Gene Summary: This gene encodes a ribonuclease (RNase) III double-stranded RNA-specific ribonuclease and subunit of the microprocessor protein complex, which catalyzes the initial processing step of microRNA (miRNA) synthesis. The encoded protein cleaves the stem loop structure from the primary microRNA (pri-miRNA) in the nucleus, yielding the precursor miRNA (pre-miRNA), which is then exported to the cytoplasm for further processing. In a human cell line lacking a functional copy of this gene, canonical miRNA synthesis is reduced. Somatic mutations in this gene have been observed in human patients with kidney cancer. [provided by RefSeq, Sep 2016]