DROSHA (NM_001100412) Human Untagged Clone

CAT#: SC316870

DROSHA (untagged)-Human drosha, ribonuclease type III (DROSHA), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DROSHA
• Synonyms: ETOHI2; HSA242976; RANSE3L; RN3; RNASE3L; RNASEN
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001100412, the custom clone sequence may differ by one or more nucleotides
  ATGATGCAGGGAAACACATGTCACAGAATGTCGTTCCACCCGGGACGAGGGTGTCCCCGA
  GGACGAGGAGGACATGGAGCCAGACCCTCAGCACCATCCTTTAGGCCCCAAAATCTGAGG
  CTGCTTCACCCTCAGCAGCCTCCTGTGCAATATCAATATGAACCTCCAAGTGCCCCTTCC
  ACCACTTTCTCAAACTCTCCAGCCCCCAATTTTCTCCCTCCACGACCAGACTTTGTACCC
  TTCCCCCCACCCATGCCTCCGTCAGCGCAAGGCCCTCTTCCCCCCTGCCCAATCAGGCCG
  CCTTTCCCCAACCACCAGATGAGGCACCCCTTCCCAGTTCCTCCTTGTTTTCCTCCCATG
  CCACCACCAATGCCTTGTCCTAATAACCCCCCAGTCCCTGGGGCACCTCCTGGACAAGGC
  ACTTTCCCCTTCATGATGCCCCCTCCCTCCATGCCTCATCCCCCGCCCCCTCCAGTCATG
  CCGCAGCAGGTTAATTATCAGTACCCTCCGGGCTATTCTCACCACAACTTCCCACCTCCC
  AGTTTTAATAGTTTCCAGAACAACCCTAGTTCTTTCCTGCCCAGTGCTAATAACAGCAGT
  AGTCCTCATTTCAGACATCTCCCTCCATACCCACTCCCAAAGGCTCCCAGTGAGAGAAGG
  TCCCCAGAAAGGCTGAAACACTATGATGACCACAGGCACCGAGATCACAGTCATGGGCGA
  GGTGAGAGGCATCGGTCCCTGGATCGGCGGGAGCGAGGCCGCAGTCCCGACAGGAGAAGA
  CAAGACAGCCGGTACAGATCTGATTATGACCGAGGGAGAACACCATCTCGCCACCGCAGC
  TACGAACGGAGCAGAGAGCGAGAACGGGAGAGACACAGGCATCGAGACAACCGAAGATCA
  CCATCTCTGGAAAGGTCCTACAAAAAAGAGTATAAGAGATCTGGAAGTCGCTCCCCAAGT
  AGGGAGAAGAAGAGAGCTCGTTGGGAGGAAGAAAAAGACCGTTGGAGTGACAACCAGAGT
  TCTGGCAAAGACAAGAACTATACCTCAATCAAGGAAAAAGAGCCCGAGGAGACCATGCCT
  GACAAGAATGAGGAGGAAGAAGAAGAACTTCTTAAGCCTGTGTGGATTCGATGCACTCAT
  TCAGAAAACTACTACTCCAGTGACCCCATGGATCAGGTGGGAGATTCTACAGTGGTTGGA
  ACGAGTAGGCTTCGTGACTTATATGACAAATTTGAGGAGGAGTTGGGGAGCAGGCAAGAA
  AAGGCCAAAGCTGCTCGGCCTCCGTGGGAACCTCCAAAGACGAAGCTCGATGAAGATTTA
  GAGAGTTCCAGTGAATCCGAGTGTGAGTCTGATGAGGACAGCACCTGTTCTAGCAGCTCA
  GACTCTGAAGTTTTTGACGTTATTGCAGAAATCAAACGCAAAAAGGCCCACCCTGACCGA
  CTTCATGATGAACTTTGGTACAACGATCCAGGCCAGATGAATGATGGACCACTCTGCAAA
  TGCAGCGCAAAGGCAAGACGCACAGGAATTAGGCACAGCATTTATCCTGGAGAAGAGGCC
  ATCAAGCCCTGTCGTCCTATGACCAACAATGCTGGCAGACTTTTCCACTACCGGATCACA
  GTCTCCCCGCCTACGAACTTTTTAACTGACAGGCCAACTGTTATAGAATACGATGATCAC
  GAGTATATCTTTGAAGGATTTTCTATGTTTGCACATGCCCCCCTGACCAATATTCCACTG
  TGTAAAGTAATTAGATTCAACATAGACTACACGATTCATTTCATTGAAGAGATGATGCCG
  GAGAATTTTTGTGTGAAAGGGCTTGAACTCTTTTCACTGTTCCTATTCAGAGATATTTTG
  GAATTATATGACTGGAATCTTAAAGGTCCTTTGTTTGAAGACAGCCCTCCCTGCTGCCCA
  AGATTTCATTTCATGCCACGTTTTGTAAGATTTCTTCCAGATGGAGGAAAGGAAGTGCTG
  TCCATGCACCAGATTCTCCTGTACTTGTTAAGGTGCAGCAAAGCCCTGGTGCCTGAGGAG
  GAGATTGCCAATATGCTTCAGTGGGAGGAGCTGGAGTGGCAGAAATATGCAGAAGAATGC
  AAAGGCATGATTGTTACCAACCCTGGGACGAAACCAAGCTCTGTCCGTATCGATCAACTG
  GATCGTGAACAGTTCAACCCCGATGTGATTACTTTTCCGATTATCGTCCACTTTGGGATA
  CGCCCTGCACAGTTGAGTTATGCAGGAGACCCACAGTACCAAAAACTGTGGAAGAGTTAT
  GTGAAACTTCGCCACCTCCTAGCAAATAGTCCCAAAGTCAAACAAACTGACAAACAGAAG
  CTGGCACAGAGGGAGGAAGCCCTCCAAAAAATACGGCAGAAGAATACAATGAGACGAGAA
  GTAACGGTGGAGCTAAGTAGCCAAGGATTCTGGAAAACTGGCATCCGTTCTGATGTCTGT
  CAGCATGCAATGATGCTACCTGTTCTGACCCATCATATCCGCTACCACCAATGCCTAATG
  CATTTGGACAAGTTGATAGGATATACTTTCCAAGATCGTTGTCTGTTGCAGCTGGCCATG
  ACTCATCCAAGTCATCATTTAAATTTTGGAATGAATCCTGATCATGCCAGGAATTCATTA
  TCTAACTGTGGAATTCGGCAGCCCAAATACGGAGACAGAAAAGTTCATCACATGCACATG
  CGGAAGAAAGGGATTAACACCTTGATAAATATCATGTCACGCCTTGGCCAAGATGACCCA
  ACTCCCTCGAGGATTAACCACAATGAACGGTTGGAATTCCTGGGTGATGCTGTTGTTGAA
  TTTCTGACCAGCGTCCATTTGTACTATTTGTTTCCTAGTCTGGAAGAAGGAGGATTAGCA
  ACCTATCGGACTGCCATTGTTCAGAATCAGCACCTTGCCATGCTAGCAAAGAAACTTGAA
  CTGGATCGATTTATGCTGTATGCTCACGGGCCTGACCTTTGTAGAGAATCGGACCTTCGA
  CATGCAATGGCCAATTGTTTTGAAGCGTTAATAGGAGCTGTTTACTTGGAGGGAAGCCTG
  GAGGAAGCCAAGCAGTTATTTGGACGCTTGCTCTTTAATGATCCGGACCTGCGCGAAGTC
  TGGCTCAATTATCCTCTCCACCCACTCCAACTACAAGAGCCAAATACTGATCGACAACTT
  ATTGAAACTTCTCCAGTTCTACAAAAACTTACTGAGTTTGAAGAAGCAATTGGAGTAATT
  TTTACTCATGTTCGACTTCTGGCAAGGGCATTCACATTGAGAACTGTGGGATTTAACCAT
  CTGACCCTAGGCCACAATCAGAGAATGGAATTCCTAGGTGACTCCATAATGCAACTGGTA
  GCCACAGAGTACTTATTCATTCATTTCCCAGATCATCATGAAGGACACTTAACTTTGTTG
  CGAAGCTCTTTGGTGAATAATAGAACTCAGGCCAAGGTAGCGGAGGAGCTGGGCATGCAG
  GAGTACGCCATAACCAACGACAAGACCAAGAGGCCTGTGGCGCTTCGCACCAAGACCTTG
  GCGGACCTTTTGGAATCATTTATTGCAGCGCTGTACATTGATAAGGATTTGGAATATGTT
  CATACTTTCATGAATGTCTGCTTCTTTCCACGATTGAAAGAGTTCATTTTGAATCAGGAT
  TGGAATGACCCCAAATCCCAGCTTCAGCAGTGTTGCTTGACACTTAGGACAGAAGGAAAA
  GAGCCAGACATTCCTCTGTACAAGACTCTGCAGACAGTGGGCCCATCCCATGCCCGAACC
  TACACTGTGGCTGTTTATTTCAAGGGAGAAAGAATAGGCTGTGGGAAAGGACCAAGTATT
  CAGCAAGCGGAAATGGGAGCAGCAATGGATGCGCTTGAAAAATATAATTTTCCCCAGATG
  GCCCATCAGAAGCGGTTCATCGAACGGAAGTACAGACAAGAGTTAAAAGAAATGAGGTGG
  GAAAGAGAGCATCAAGAGAGAGAGCCAGATGAGACTGAAGACATCAAGAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001100412
• ORF Size: 4014 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_001100412.1, NP_001093882.1
• RefSeq Size: 5389
• RefSeq ORF: 4014
• Locus ID: 29102
• Gene Summary: This gene encodes a ribonuclease (RNase) III double-stranded RNA-specific ribonuclease and subunit of the microprocessor protein complex, which catalyzes the initial processing step of microRNA (miRNA) synthesis. The encoded protein cleaves the stem loop structure from the primary microRNA (pri-miRNA) in the nucleus, yielding the precursor miRNA (pre-miRNA), which is then exported to the cytoplasm for further processing. In a human cell line lacking a functional copy of this gene, canonical miRNA synthesis is reduced. Somatic mutations in this gene have been observed in human patients with kidney cancer. [provided by RefSeq, Sep 2016]
  Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. The resulting protein (isoform 2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.