Orexin (HCRT) (NM_001524) Human Tagged ORF Clone
CAT#: RC217956
HCRT (Myc-DDK-tagged)-Human hypocretin (orexin) neuropeptide precursor (HCRT)
"NM_001524" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | HCRT |
Synonyms | NRCLP1; OX; PPOX |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC217956 representing NM_001524
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACCTTCCTTCCACAAAGGTCTCCTGGGCCGCCGTGACGCTACTGCTGCTGCTGCTGCTGCTGCCGC CCGCGCTGTTGTCGTCCGGGGCGGCTGCACAGCCCCTGCCCGACTGCTGTCGTCAAAAGACTTGCTCTTG CCGCCTCTACGAGCTGCTGCACGGCGCGGGCAATCACGCGGCCGGCATCCTCACGCTGGGCAAGCGGAGG TCCGGGCCCCCGGGCCTCCAGGGTCGGCTGCAGCGCCTCCTGCAGGCCAGCGGCAACCACGCCGCGGGCA TCCTGACCATGGGCCGCCGCGCAGGCGCAGAGCCAGCGCCGCGCCCCTGCCTCGGGCGCCGCTGTTCCGC CCCGGCCGCCGCCTCCGTCGCGCCCGGAGGACAGTCCGGGATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC217956 representing NM_001524
Red=Cloning site Green=Tags(s) MNLPSTKVSWAAVTLLLLLLLLPPALLSSGAAAQPLPDCCRQKTCSCRLYELLHGAGNHAAGILTLGKRR SGPPGLQGRLQRLLQASGNHAAGILTMGRRAGAEPAPRPCLGRRCSAPAAASVAPGGQSGI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001524 |
ORF Size | 393 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001524.1, NP_001515.1 |
RefSeq Size | 577 bp |
RefSeq ORF | 396 bp |
Locus ID | 3060 |
Cytogenetics | 17q21.2 |
Protein Families | Druggable Genome, Transmembrane |
MW | 13.2 kDa |
Gene Summary | 'This gene encodes a hypothalamic neuropeptide precursor protein that gives rise to two mature neuropeptides, orexin A and orexin B, by proteolytic processing. Orexin A and orexin B, which bind to orphan G-protein coupled receptors HCRTR1 and HCRTR2, function in the regulation of sleep and arousal. This neuropeptide arrangement may also play a role in feeding behavior, metabolism, and homeostasis. [provided by RefSeq, Jan 2010]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC303041 | HCRT (untagged)-Human hypocretin (orexin) neuropeptide precursor (HCRT) |
USD 420.00 |
|
RG217956 | HCRT (GFP-tagged) - Human hypocretin (orexin) neuropeptide precursor (HCRT) |
USD 460.00 |
|
RC217956L1 | Lenti ORF clone of Human hypocretin (orexin) neuropeptide precursor (HCRT), Myc-DDK-tagged |
USD 768.00 |
|
RC217956L2 | Lenti ORF clone of Human hypocretin (orexin) neuropeptide precursor (HCRT), mGFP tagged |
USD 620.00 |
|
RC217956L3 | Lenti ORF clone of Human hypocretin (orexin) neuropeptide precursor (HCRT), Myc-DDK-tagged |
USD 620.00 |
|
RC217956L4 | Lenti ORF clone of Human hypocretin (orexin) neuropeptide precursor (HCRT), mGFP tagged |
USD 620.00 |
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