NAGPA (NM_016256) Human Tagged ORF Clone

CAT#: RC218271

NAGPA (Myc-DDK-tagged)-Human N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA)

Product Images

Western blot validation of overexpression lysate (Cat# LY414096) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218271 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified NAGPA protein (Cat# TP318271). The protein was produced from HEK293T cells transfected with NAGPA cDNA clone (Cat# RC218271) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NAGPA
• Synonyms: APAA; UCE
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218271 representing NM_016256
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACCTCCACGGGTCGCTGGCTTCTCCTCCGGCTTGCACTATTCGGCTTCCTCTGGGAAGCGTCCG
  GCGGCCTCGACTCGGGGGCCTCCCGCGACGACGACTTGCTACTGCCCTATCCACGCGCGCGCGCGCGCCT
  CCCCCGGGACTGCACACGGGTGCGCGCCGGCAACCGCGAGCACGAGAGTTGGCCTCCGCCTCCCGCGACT
  CCCGGCGCCGGCGGTCTGGCCGTGCGCACCTTCGTGTCGCACTTCAGGGACCGCGCGGTGGCCGGCCACC
  TGACGCGGGCCGTTGAGCCCCTGCGCACCTTCTCGGTGCTGGAGCCCGGTGGACCCGGCGGCTGCGCGGC
  GAGACGACGCGCCACCGTGGAGGAGACGGCGCGGGCGGCCGACTGCCGTGTCGCCCAGAACGGCGGCTTC
  TTCCGCATGAACTCGGGCGAGTGCCTGGGGAACGTGGTGAGCGACGAGCGGCGGGTGAGCAGCTCCGGGG
  GGCTGCAGAACGCGCAGTTCGGGATCCGCCGCGACGGGACCCTGGTCACCGGGTACCTGTCTGAGGAGGA
  GGTGCTGGACACTGAGAACCCATTTGTGCAGCTGCTGAGTGGGGTCGTGTGGCTGATTCGTAATGGAAGC
  ATCTACATCAACGAGAGCCAAGCCACAGAGTGTGACGAGACACAGGAGACAGGTTCCTTTAGCAAATTTG
  TGAATGTGATATCAGCCAGGACGGCCATTGGCCACGACCGGAAAGGGCAGCTGGTGCTCTTTCATGCAGA
  CGGCCAAACGGAGCAGCGTGGCATCAACCTGTGGGAAATGGCGGAGTTCCTGCTGAAACAGGACGTGGTC
  AACGCCATCAACCTGGATGGGGGTGGCTCTGCCACCTTTGTGCTCAACGGGACCTTGGCCAGTTACCCGT
  CAGATCACTGCCAGGACAACATGTGGCGCTGTCCCCGCCAAGTGTCCACCGTGGTGTGTGTGCACGAACC
  CCGCTGCCAGCCGCCTGACTGCCACGGCCACGGGACCTGCGTGGACGGGTACTGCCAATGCACCGGGCAC
  TTCTGGCGGGGTCCCGGCTGTGATGAGCTGGACTGTGGCCCCTCTAACTGCAGCCAGCACGGACTGTGCA
  CGGAGACCGGCTGCCGCTGTGATGCCGGATGGACCGGGTCCAACTGCAGTGAAGAGTGTCCCCTTGGCTG
  GCATGGGCCGGGCTGCCAGAGGCCTTGTAAGTGTGAGCACCATTGTCCCTGTGACCCCAAGACTGGCAAC
  TGCAGCGTCTCCAGAGTAAAGCAGTGTCTCCAGCCACCTGAAGCCACCCTGAGGGCGGGAGAACTCTCCT
  TTTTCACCAGGACCGCCTGGCTAGCCCTCACCCTGGCGCTGGCCTTCCTCCTGCTGATCAGCATTGCAGC
  AAACCTGTCCTTGCTCCTGTCCAGAGCAGAGAGGAACCGGCGCCTGCATGGGGACTATGCATACCACCCG
  CTGCAGGAGATGAACGGGGAGCCTCTGGCCGCAGAGAAGGAGCAGCCAGGGGGCGCCCACAACCCCTTCA
  AGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218271 representing NM_016256
  Red=Cloning site Green=Tags(s)
  
  MATSTGRWLLLRLALFGFLWEASGGLDSGASRDDDLLLPYPRARARLPRDCTRVRAGNREHESWPPPPAT
  PGAGGLAVRTFVSHFRDRAVAGHLTRAVEPLRTFSVLEPGGPGGCAARRRATVEETARAADCRVAQNGGF
  FRMNSGECLGNVVSDERRVSSSGGLQNAQFGIRRDGTLVTGYLSEEEVLDTENPFVQLLSGVVWLIRNGS
  IYINESQATECDETQETGSFSKFVNVISARTAIGHDRKGQLVLFHADGQTEQRGINLWEMAEFLLKQDVV
  NAINLDGGGSATFVLNGTLASYPSDHCQDNMWRCPRQVSTVVCVHEPRCQPPDCHGHGTCVDGYCQCTGH
  FWRGPGCDELDCGPSNCSQHGLCTETGCRCDAGWTGSNCSEECPLGWHGPGCQRPCKCEHHCPCDPKTGN
  CSVSRVKQCLQPPEATLRAGELSFFTRTAWLALTLALAFLLLISIAANLSLLLSRAERNRRLHGDYAYHP
  LQEMNGEPLAAEKEQPGGAHNPFKD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016256
• ORF Size: 1545 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016256.1, NM_016256.2, NM_016256.3, NP_057340.2
• RefSeq Size: 2219 bp
• RefSeq ORF: 1548 bp
• Locus ID: 51172
• Domains: EGF
• Protein Families: Transmembrane
• Protein Pathways: Lysosome
• MW: 56.11 kDa
• Gene Summary: Hydrolases are transported to lysosomes after binding to mannose 6-phosphate receptors in the trans-Golgi network. This gene encodes the enzyme that catalyzes the second step in the formation of the mannose 6-phosphate recognition marker on lysosomal hydrolases. Commonly known as 'uncovering enzyme' or UCE, this enzyme removes N-acetyl-D-glucosamine (GlcNAc) residues from GlcNAc-alpha-P-mannose moieties and thereby produces the recognition marker. The encoded preproprotein is proteolytically processed by furin to generate the mature enzyme, a homotetramer of two disulfide-linked homodimers. Mutations in this gene are associated with developmental stuttering in human patients. [provided by RefSeq, Oct 2015]