NAGPA (NM_016256) Human Untagged Clone

CAT#: SC114360

NAGPA (untagged)-Human N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NAGPA
• Synonyms: APAA; UCE
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114360 sequence for NM_016256 edited (data generated by NextGen Sequencing)
  ATGGCGACCTCCACGGGTCGCTGGCTTCTCCTCCGGCTTGCACTATTCGGCTTCCTCTGG
  GAAGCGTCCGGCGGCCTCGACTCGGGGGCCTCCCGCGACGACGACTTGCTACTGCCCTAT
  CCACGCGCGCGCGCGCGCCTCCCCCGGGACTGCACACGGGTGCGCGCCGGCAACCGCGAG
  CACGAGAGTTGGCCTCCGCCTCCCGCGACTCCCGGCGCCGGCGGTCTGGCCGTGCGCACC
  TTCGTGTCGCACTTCAGGGACCGCGCGGTGGCCGGCCACCTGACGCGGGCCGTTGAGCCC
  CTGCGCACCTTCTCGGTGCTGGAGCCCGGTGGACCCGGCGGCTGCGCGGCGAGACGACGC
  GCCACCGTGGAGGAGACGGCGCGGGCGGCCGACTGCCGTGTCGCCCAGAACGGCGGCTTC
  TTCCGCATGAACTCGGGCGAGTGCCTGGGGAACGTGGTGAGCGACGAGCGGCGGGTGAGC
  AGCTCCGGGGGGCTGCAGAACGCGCAGTTCGGGATCCGCCGCGACGGGACCCTGGTCACC
  GGGTACCTGTCTGAGGAGGAGGTGCTGGACACTGAGAACCCATTTGTGCAGCTGCTGAGT
  GGGGTCGTGTGGCTGATTCGTAATGGAAGCATCTACATCAACGAGAGCCAAGCCACAGAG
  TGTGACGAGACACAGGAGACAGGTTCCTTTAGCAAATTTGTGAATGTGATATCAGCCAGG
  ACGGCCATTGGCCACGACCGGAAAGGGCAGCTGGTGCTCTTTCATGCAGACGGCCAAACG
  GAGCAGCGTGGCATCAACCTGTGGGAAATGGCGGAGTTCCTGCTGAAACAGGACGTGGTC
  AACGCCATCAACCTGGATGGGGGTGGCTCTGCCACCTTTGTGCTCAACGGGACCTTGGCC
  AGTTACCCGTCAGATCACTGCCAGGACAACATGTGGCGCTGTCCCCGCCAAGTGTCCACC
  GTGGTGTGTGTGCACGAACCCCGCTGCCAGCCGCCTGACTGCCACGGCCACGGGACCTGC
  GTGGACGGGTACTGCCAATGCACCGGGCACTTCTGGCGGGGTCCCGGCTGTGATGAGCTG
  GACTGTGGCCCCTCTAACTGCAGCCAGCACGGACTGTGCACGGAGACCGGCTGCCGCTGT
  GATGCCGGATGGACCGGGTCCAACTGCAGTGAAGAGTGTCCCCTTGGCTGGCATGGGCCG
  GGCTGCCAGAGGCCTTGTAAGTGTGAGCACCATTGTCCCTGTGACCCCAAGACTGGCAAC
  TGCAGCGTCTCCAGAGTAAAGCAGTGTCTCCAGCCACCTGAAGCCACCCTGAGGGCGGGA
  GAACTCTCCTTTTTCACCAGGACCGCCTGGCTAGCCCTCACCCTGGCGCTGGCCTTCCTC
  CTGCTGATCAGCATTGCAGCAAACCTGTCCTTGCTCCTGTCCAGAGCAGAGAGGAACCGG
  CGCCTGCATGGGGACTATGCATACCACCCGCTGCAGGAGATGAACGGGGAGCCTCTGGCC
  GCAGAGAAGGAGCAGCCAGGGGGCGCCCACAACCCCTTCAAGGACTGA
  
  Clone variation with respect to NM_016256.3
  1030 c=>t;1394 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016256 unedited
  GTTCACGAATTTGTAATACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGGAATAT
  GGCGACCTCCAGGGTCGCTGGCTTCTCCTCCGGCTTGCACTATTCGGCTTCCTCTGGGAA
  GCGTCCGGCGGCCTCGACTCGGGGGCCTCCCGCGACGACGACTTGCTACTGCCCTATCCA
  CGCGCGCGCGCGCGCCTCCCCCGGGACTGCACACGGGTGCGCGCCGGCAACCGCGAGCAC
  GAGAGTTGGCCTCCGCCTCCCGCGACTCCCGGCGCCGGCGGTCTGGCCGTGCGCACCTTC
  GTGTCGCACTTCAGGGACCGCGCGGTGGCCGGCCACCTGACGCGGGCCGTTGAGCCCCTG
  CGCACCTTCTCGGTGCTGGAGCCCGGTGGACCCGGCGGCTGCGCGGCGAGACGACGCGCC
  ACCGTGGAGGAGACGGCGCGGGCGGCCGACTGCCGTGTCGCCCAGAACGGCGGCTTCTTC
  CGCATGAACTCGGGCGAGTGCCTGGGGAACGTGGTGAGCGACGAGCGGCGGGTGAGCAGC
  TCCGGGGGGCTGCAGAACGCGCAGTTCGGGATCCGCCGCGACGGGACCCTGGTCACCGGG
  TACCTGTCTGAGGAGGAGGTGCTGGACACTGAGAACCCATNTGTGCAGCTGCTGAGTGGG
  GTCGTGTGGCTGATTCGTAATGGAAGCATCTACATCAACGAGAGCCAAGCCACAGAGTGT
  GACGAGACACAGGAGACAGGTTCCTTTAGCAAATTTGTGAATGTGATATCAGCCAGGACG
  GCCATTGGCCACGACCGGNAAGGGCAGCTGGTGCTCTTTCATGCAGACGGGCAAACGGAG
  CAGCGTGGCATCAACCTGTGGGAAATGGCNNGAGTNCTGCTGAANCAGACGTGGTCACGC
  ATCACCGTGAGGGGGGGGGCTCTGCCACCTTGTGCTCACG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016256 unedited
  GGGGGGCCGANGNNAGNNNNTTTTNNNNNNGNNTTTCNGGNACCGGAGCCGCATTTTATA
  TNCGGTTTTTTTTTCTTTTTTTTTCCTTTTATTATATCAATTTATTCAAGTGATTAAAAA
  ACTTCTTGGCAGCAGGATGACAAAACAGGTCTGTAAACATGATTTCATCCCCCCATCCCT
  TCTTCCAAGGAGCTTCCTATGACAGGAAATCGAATTCTAGTTGGCAAATCCAGTGATGAG
  GTCTGTAGAAAAGGGGTCCCGTGTCACAGCCAGGAAGGCAGACTCGTCCCTTTAACCCAC
  TCCAGCCAGGGGTGCTGGCCAGGATGTGCTGTTCTGTCATGACGTGGTCACTGAGTCTGG
  TTCGTTGGCAGCCCCTTCCCCAGGAGGAGGGAGACTCTTTGGCAGTGCGGCAGCCCTCCC
  GGGGGCACGGGCTTCTCGGCAGCAGACACAGGCAGGCGAGAAGCAGGCAGCTGAGCCTCT
  CCAGCGAGCATGGTATTGCTAGGGTTGCAGGTGCCCTGNCAGGTGGCCAGGTGAGGGGCT
  GAGGCACAAGTGCTATCAGGAACTTGGCTGCCCCACAGGGGCTGANGACACCCAGATGGT
  CCACGCCAGTGGCCTTGAAATTTCCCCTGCAGAAGCCAGACCGTGGGGAAACAAGCTNTC
  GCGACGTGCCACCCCCGGCAGCTTGAGGCTTCAGTCCTTGAGGGGGNTGTGGGCGCCCCC
  TGGNCTGCTCTTCTCTGCGGCCAGAAGCTCCCCGTNCATCTCCTGCAGCCGGTGGTATGC
  ATAGNTCCCATGCNAGCGCCGGTTCCTCTCTGCTCTGGACAGAGCAAGACAGGNTTGCTG
  CATGCTGATCNCAAGAGGAAGCCACCCCCGGTGAGGGCTACCCAGCCGCCCTGTGAAAAA
  GAAAGTCCTCCGCCTCAAGGGGGCTAAGGTGCTGGAAACATGTTTACTTTGAAACCCTTC
  ANTGGCAATCTGGGGTCACAGGCATGGGGCCCACACTACAAGCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_016256
• ORF Size: 1548 bp
• Insert Size: 2290
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_016256.2, NP_057340.2
• RefSeq Size: 2219
• RefSeq ORF: 1548
• Locus ID: 51172
• Domains: EGF
• Protein Families: Transmembrane
• Protein Pathways: Lysosome
• Gene Summary: Hydrolases are transported to lysosomes after binding to mannose 6-phosphate receptors in the trans-Golgi network. This gene encodes the enzyme that catalyzes the second step in the formation of the mannose 6-phosphate recognition marker on lysosomal hydrolases. Commonly known as 'uncovering enzyme' or UCE, this enzyme removes N-acetyl-D-glucosamine (GlcNAc) residues from GlcNAc-alpha-P-mannose moieties and thereby produces the recognition marker. The encoded preproprotein is proteolytically processed by furin to generate the mature enzyme, a homotetramer of two disulfide-linked homodimers. Mutations in this gene are associated with developmental stuttering in human patients. [provided by RefSeq, Oct 2015]