PCDHA1 (NM_018900) Human Tagged ORF Clone

CAT#: RC218500

PCDHA1 (Myc-DDK-tagged)-Human protocadherin alpha 1 (PCDHA1), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PCDHA1
• Synonyms: PCDH-ALPHA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218500 representing NM_018900
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGTTTTCTAGGAGAGGGGGCCTGGGAGCCCGGGATCTGCTTCTTTGGCTTCTGCTCCTCGCAGCCT
  GGGAGGTGGGGAGCGGCCAGCTCCACTACTCGATCCCGGAGGAAGCCAAACACGGCACCTTCGTTGGCCG
  CGTTGCTCAGGACCTGGGACTGGAGCTGGCGGAGCTGGTGCCTCGCCTGTTCCGGGTGGCGTCCAAAACA
  CACAGGGACCTTCTGGAGGTAAATCTGCAGAATGGCATTTTGTTTGTGAATTCTCGGATCGATCGCGAGG
  AGCTGTGCCAGTGGAGCGCGGAGTGCAGCATCCACCTGGAGTTGATCGCCGACAGGCCGCTGCAGGTTTT
  CCATGTGGAGGTGAAGGTGAAAGACATTAACGATAATCCACCCGTCTTCAGGGGCAGAGAACAAATAATA
  TTTATTCCTGAATCTAGACTCCTGAATTCGCGTTTTCCGATAGAAGGAGCTGCTGATGCAGACATTGGTG
  CTAACGCTCTTCTAACGTACACGCTCAGCCCGAGTGATTATTTCTCTTTGGATGTAGAGGCAAGTGATGA
  ACTGAGTAAATCTCTTTGGCTTGAATTGAGAAAATATTTGGATAGAGAAGAAACACCAGAACTTCACTTA
  TTACTGACTGCCACTGATGGGGGCAAACCGGAGCTGCAAGGTACAGTTGAGCTGCTGATCACCGTCCTCG
  ACGTTAATGATAACGCCCCACTGTTTGACCAGGCCGTATACAGAGTCCACTTGTTAGAGACTACAGCAAA
  TGGAACATTAGTGACCACATTAAATGCCTCTGATGCTGACGAAGGTGTAAATGGTGAAGTCGTCTTTTCC
  TTTGACAGTGGTATTTCTCGTGACATTCAAGAAAAATTCAAAGTTGATTCCAGCTCAGGAGAAATTAGGT
  TAATTGATAAACTGGATTATGAAGAAACAAAATCCTACGAAATTCAAGTAAAGGCAGTTGATAAAGGAAG
  TCCTCCGATGTCAAATCACTGTAAGGTTTTGGTGAAAGTGCTGGATGTAAATGATAATGCTCCAGAACTG
  GCGGTCACTTCATTGTATTTGCCTATCAGAGAGGACGCTCCACTCAGCACCGTCATCGCCCTCATCACCG
  TGTCTGACCGTGACTCAGGTGCCAACGGGCAGGTGACTTGCTCCTTAATGCCCCACGTCCCCTTCAAGCT
  GGTGTCCACCTTCAAGAATTACTACTCGTTGGTGTTGGACAGCGCCCTGGATCGCGAGAGCCTGTCGGTC
  TATGAGCTGGTGGTGACCGCGCGGGACGGGGGCTCGCCTTCGCTGTGGGCCACGGCCAGGGTGTCCGTGG
  AGGTGGCCGACGTGAATGACAACGCGCCTGCGTTCGCGCAGCCCGAGTACACAGTATTCGTGAAGGAGAA
  CAACCCGCCGGGCTGCCACATCTTCACGGTGTCTGCGCGGGACGCGGACGCGCAGGAGAACGCGCTGGTG
  TCCTATTCGCTGGTGGAACGGCGGGTGGGCGAGCGCGCGCTGTCGAACTACGTGTCAGTGCACGCGGAGA
  GCGGCAAGGTGTACGCACTGCAGCCCCTGGACCACGAGGAGCTGGAGCTGCTGCAGTTCCAGGTGAGCGC
  GCGGGATGCGGGCGTGCCGCCTCTGGGCAGCAACGTGACGCTGCAGGTGTTCGTGCTGGACGAGAACGAC
  AACGCGCCGGCGCTGCTGGCGCCTCGAGTGGGTGGCACTATTGGTGCAGTCAGTGAGCTGGTGCCGCGAT
  TGGTGGGTGCGGGTCATGTGGTGGCGAAGGTGCGCGCAGTGGACGCCGACTCGGGCTACAACGCGTGGCT
  GTCCTATGAACTGCAGCCGGCAGCAGGCGGCGCGCGCATCCCGTTCCGCGTGGGGCTGTACACGGGCGAG
  ATCAGCACGACTCGTGTCCTGGACGAGGCTGACTTGTCGCGCTACCGCCTTCTGGTGCTAGTGAAGGATC
  ACGGTGAGCCGGCGCTGACAGCCACGGCCACTGTGCTTGTATCTCTGGTGGAGAGCGGCCAGGCGCCAAA
  GGCGTCTTCGCGGGCGTCGGTGGGTGTCGCGGGCCCAGAGGCGGCGCTGGTGGATGTCAACGTGTACCTG
  ATCATCGCCATCTGCGCGGTGTCCAGCCTGCTGGTGCTCACACTGCTGCTGTACACGGCGCTGCGGTGCT
  CAGTGCCGCCCACTGAGGGTGCGTATGTGCCGGGCAAGCCCACTCTGGTGTGCTCCAGCGCGTTGGGGAG
  CTGGTCGAACTCACAGCAGAGGCGGCAGAGGGTGTGCTCTAGCGAGGGCCCACCCAAGACCGACCTCATG
  GCCTTCAGCCCAGGCCTATCTCCAAGTCTTAACACGTCAGAAAGAAATGAACAACCAGAAGCAAATTTGG
  ATCTTTCTGGTAATCCACGACAGCCCAACCCTGACTGGCGTTACTCTGCCTCCCTGAGAGCAGGCATGCA
  CAGCTCTGTGCACCTAGAGGAGGCTGGCATTCTACGGGCTGGTCCAGGAGGGCCTGATCAGCAGTGGCCA
  ACAGTATCCAGTGCAACACCAGAACCAGAGGCAGGAGAAGTGTCCCCTCCAGTCGGTGCGGGTGTCAACA
  GCAACAGCTGGACCTTTAAATACGGACCAGGCAACCCCAAACAATCCGGTCCCGGTGAGTTGCCCGACAA
  ATTCATTATCCCAGGATCTCCTGCAATCATCTCCATCCGGCAGGAGCCTACTAACAGCCAAATTGACAAA
  AGTGACTTCATAACCTTCGGCAAAAAGGAGGAGACCAAGAAAAAGAAGAAAAAGAAGAAGGGTAACAAGA
  CCCAGGAGAAAAAAGAGAAAGGGAACAGCACGACTGACAACAGTGACCAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218500 representing NM_018900
  Red=Cloning site Green=Tags(s)
  
  MVFSRRGGLGARDLLLWLLLLAAWEVGSGQLHYSIPEEAKHGTFVGRVAQDLGLELAELVPRLFRVASKT
  HRDLLEVNLQNGILFVNSRIDREELCQWSAECSIHLELIADRPLQVFHVEVKVKDINDNPPVFRGREQII
  FIPESRLLNSRFPIEGAADADIGANALLTYTLSPSDYFSLDVEASDELSKSLWLELRKYLDREETPELHL
  LLTATDGGKPELQGTVELLITVLDVNDNAPLFDQAVYRVHLLETTANGTLVTTLNASDADEGVNGEVVFS
  FDSGISRDIQEKFKVDSSSGEIRLIDKLDYEETKSYEIQVKAVDKGSPPMSNHCKVLVKVLDVNDNAPEL
  AVTSLYLPIREDAPLSTVIALITVSDRDSGANGQVTCSLMPHVPFKLVSTFKNYYSLVLDSALDRESLSV
  YELVVTARDGGSPSLWATARVSVEVADVNDNAPAFAQPEYTVFVKENNPPGCHIFTVSARDADAQENALV
  SYSLVERRVGERALSNYVSVHAESGKVYALQPLDHEELELLQFQVSARDAGVPPLGSNVTLQVFVLDEND
  NAPALLAPRVGGTIGAVSELVPRLVGAGHVVAKVRAVDADSGYNAWLSYELQPAAGGARIPFRVGLYTGE
  ISTTRVLDEADLSRYRLLVLVKDHGEPALTATATVLVSLVESGQAPKASSRASVGVAGPEAALVDVNVYL
  IIAICAVSSLLVLTLLLYTALRCSVPPTEGAYVPGKPTLVCSSALGSWSNSQQRRQRVCSSEGPPKTDLM
  AFSPGLSPSLNTSERNEQPEANLDLSGNPRQPNPDWRYSASLRAGMHSSVHLEEAGILRAGPGGPDQQWP
  TVSSATPEPEAGEVSPPVGAGVNSNSWTFKYGPGNPKQSGPGELPDKFIIPGSPAIISIRQEPTNSQIDK
  SDFITFGKKEETKKKKKKKKGNKTQEKKEKGNSTTDNSDQ
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018900
• ORF Size: 2850 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_018900.1, NM_018900.2, NM_018900.3, NP_061723.1
• RefSeq Size: 5260
• RefSeq ORF: 2853
• Locus ID: 56147
• Domains: CA
• Protein Families: Transmembrane
• MW: 99.8 kDa
• Gene Summary: This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined. [provided by RefSeq, Jul 2008]