KCNQ3 (NM_004519) Human Tagged ORF Clone

CAT#: RC218739

KCNQ3 (Myc-DDK-tagged)-Human potassium voltage-gated channel, KQT-like subfamily, member 3 (KCNQ3), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY417937) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218739 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KCNQ3
• Synonyms: BFNC2; EBN2; KV7.3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218739 representing NM_004519
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCTCAAGGCGCGCAGGGCGGCGGGGGCGGCTGGCGGCGGCGGCGACGGGGGCGGCGGAGGCGGCG
  GGGCGGCTAACCCAGCCGGAGGGGACGCGGCGGCGGCCGGCGACGAGGAGCGGAAAGTGGGGCTGGCGCC
  CGGCGACGTGGAGCAAGTCACCTTGGCGCTCGGGGCCGGAGCCGACAAAGACGGGACCCTGCTGCTGGAG
  GGCGGCGGCCGCGACGAGGGGCAGCGGAGGACCCCGCAGGGCATCGGGCTCCTGGCCAAGACCCCGCTGA
  GCCGCCCAGTCAAGAGAAACAACGCCAAGTACCGGCGCATCCAAACTTTGATCTACGACGCCCTGGAGAG
  ACCGCGGGGCTGGGCGCTGCTTTACCACGCGTTGGTGTTCCTGATTGTCCTGGGGTGCTTGATTCTGGCT
  GTCCTGACCACATTCAAGGAGTATGAGACTGTCTCGGGAGACTGGCTTCTGTTACTGGAGACATTTGCTA
  TTTTCATCTTTGGAGCCGAGTTTGCTTTGAGGATCTGGGCTGCTGGATGTTGCTGCCGATACAAAGGCTG
  GCGGGGCCGACTGAAGTTTGCCAGGAAGCCCCTGTGCATGTTGGACATCTTTGTGCTGATTGCCTCTGTG
  CCAGTGGTTGCTGTGGGAAACCAAGGCAATGTTCTGGCCACCTCCCTGCGAAGCCTGCGCTTCCTGCAGA
  TCCTGCGCATGCTGCGGATGGACCGGAGAGGTGGCACCTGGAAGCTTCTGGGCTCAGCCATCTGTGCCCA
  CAGCAAAGAACTCATCACGGCCTGGTACATCGGTTTCCTGACACTCATCCTTTCTTCATTTCTTGTCTAC
  CTGGTTGAGAAAGACGTCCCAGAGGTGGATGCACAAGGAGAGGAGATGAAAGAGGAGTTTGAGACCTATG
  CAGATGCCCTGTGGTGGGGCCTGATCACACTGGCCACCATTGGCTATGGAGACAAGACACCCAAAACGTG
  GGAAGGCCGTCTGATTGCCGCCACCTTTTCCTTAATTGGCGTCTCCTTTTTTGCCCTTCCAGCGGGCATC
  CTGGGGTCCGGGCTGGCCCTCAAGGTGCAGGAGCAACACCGTCAGAAGCACTTTGAGAAAAGGAGGAAGC
  CAGCTGCTGAGCTCATTCAGGCTGCCTGGAGGTATTATGCTACCAACCCCAACAGGATTGACCTGGTGGC
  GACATGGAGATTTTATGAATCAGTCGTCTCTTTTCCTTTCTTCAGGAAAGAACAGCTGGAGGCAGCATCC
  AGCCAAAAGCTGGGTCTCTTGGATCGGGTTCGCCTTTCTAATCCTCGTGGTAGCAATACTAAAGGAAAGC
  TATTTACCCCTCTGAATGTAGATGCCATAGAAGAAAGTCCTTCTAAAGAACCAAAGCCTGTTGGCTTAAA
  CAATAAAGAGCGTTTCCGCACGGCCTTCCGCATGAAAGCCTACGCTTTCTGGCAGAGTTCTGAAGATGCC
  GGGACAGGTGACCCCATGGCGGAAGACAGGGGCTATGGGAATGACTTCCCCATCGAAGACATGATCCCCA
  CCCTGAAGGCCGCCATCCGAGCCGTCAGAATTCTACAATTCCGTCTCTATAAAAAAAAATTCAAGGAGAC
  TTTGAGGCCTTACGATGTGAAGGATGTGATTGAGCAGTATTCTGCCGGGCATCTCGACATGCTTTCCAGG
  ATAAAGTACCTTCAGACGAGAATAGATATGATTTTCACCCCTGGACCTCCCTCCACGCCAAAACACAAGA
  AGTCTCAGAAAGGGTCAGCATTCACCTTCCCATCCCAGCAATCTCCCAGGAATGAACCATATGTAGCCAG
  ACCATCCACATCAGAAATCGAAGACCAAAGCATGATGGGGAAGTTTGTAAAAGTTGAAAGACAGGTTCAG
  GACATGGGGAAGAAGCTGGACTTCCTCGTGGATATGCACATGCAACACATGGAACGGTTGCAGGTGCAGG
  TCACGGAGTATTACCCAACCAAGGGCACCTCCTCGCCAGCTGAAGCAGAGAAGAAGGAGGACAACAGGTA
  TTCCGATTTGAAAACCATCATCTGCAACTATTCTGAGACAGGCCCCCCGGAACCACCCTACAGCTTCCAC
  CAGGTGACCATTGACAAAGTCAGCCCCTATGGGTTTTTTGCACATGACCCTGTGAACCTGCCCCGAGGGG
  GACCCAGTTCTGGAAAGGTTCAGGCAACTCCTCCTTCCTCAGCAACAACGTATGTGGAGAGGCCCACGGT
  CCTGCCTATCTTGACTCTTCTCGACTCCCGAGTGAGCTGCCACTCCCAGGCTGACCTGCAGGGCCCCTAC
  TCGGACCGAATCTCCCCCCGGCAGAGACGTAGCATCACGCGAGACAGTGACACACCTCTGTCCCTGATGT
  CGGTCAACCACGAGGAGCTGGAGAGGTCTCCAAGTGGCTTCAGCATCTCCCAGGACAGAGATGATTATGT
  GTTCGGCCCCAATGGGGGGTCGAGCTGGATGAGGGAGAAGCGGTACCTCGCCGAGGGTGAGACGGACACA
  GACACGGACCCCTTCACGCCCAGCGGCTCCATGCCTCTGTCGTCCACAGGGGATGGGATTTCTGATTCAG
  TATGGACCCCTTCCAATAAGCCCATT
  
  CTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAGGATGACGACG
  ATAAGGTTTAA
  Protein Sequence

  >RC218739 representing NM_004519
  Red=Cloning site Green=Tags(s)
  
  MGLKARRAAGAAGGGGDGGGGGGGAANPAGGDAAAAGDEERKVGLAPGDVEQVTLALGAGADKDGTLLLE
  GGGRDEGQRRTPQGIGLLAKTPLSRPVKRNNAKYRRIQTLIYDALERPRGWALLYHALVFLIVLGCLILA
  VLTTFKEYETVSGDWLLLLETFAIFIFGAEFALRIWAAGCCCRYKGWRGRLKFARKPLCMLDIFVLIASV
  PVVAVGNQGNVLATSLRSLRFLQILRMLRMDRRGGTWKLLGSAICAHSKELITAWYIGFLTLILSSFLVY
  LVEKDVPEVDAQGEEMKEEFETYADALWWGLITLATIGYGDKTPKTWEGRLIAATFSLIGVSFFALPAGI
  LGSGLALKVQEQHRQKHFEKRRKPAAELIQAAWRYYATNPNRIDLVATWRFYESVVSFPFFRKEQLEAAS
  SQKLGLLDRVRLSNPRGSNTKGKLFTPLNVDAIEESPSKEPKPVGLNNKERFRTAFRMKAYAFWQSSEDA
  GTGDPMAEDRGYGNDFPIEDMIPTLKAAIRAVRILQFRLYKKKFKETLRPYDVKDVIEQYSAGHLDMLSR
  IKYLQTRIDMIFTPGPPSTPKHKKSQKGSAFTFPSQQSPRNEPYVARPSTSEIEDQSMMGKFVKVERQVQ
  DMGKKLDFLVDMHMQHMERLQVQVTEYYPTKGTSSPAEAEKKEDNRYSDLKTIICNYSETGPPEPPYSFH
  QVTIDKVSPYGFFAHDPVNLPRGGPSSGKVQATPPSSATTYVERPTVLPILTLLDSRVSCHSQADLQGPY
  SDRISPRQRRSITRDSDTPLSLMSVNHEELERSPSGFSISQDRDDYVFGPNGGSSWMREKRYLAEGETDT
  DTDPFTPSGSMPLSSTGDGISDSVWTPSNKPI
  
  LEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-XhoI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004519
• ORF Size: 2616 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004519.1, NM_004519.2, NM_004519.3, NP_004510.1
• RefSeq Size: 3097 bp
• RefSeq ORF: 2619 bp
• Locus ID: 3786
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• MW: 96.6 kDa
• Gene Summary: This gene encodes a protein that functions in the regulation of neuronal excitability. The encoded protein forms an M-channel by associating with the products of the related KCNQ2 or KCNQ5 genes, which both encode integral membrane proteins. M-channel currents are inhibited by M1 muscarinic acetylcholine receptors and are activated by retigabine, a novel anti-convulsant drug. Defects in this gene are a cause of benign familial neonatal convulsions type 2 (BFNC2), also known as epilepsy, benign neonatal type 2 (EBN2). Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2014]