Bestrophin 2 (BEST2) (NM_017682) Human Tagged ORF Clone

CAT#: RC218758

BEST2 (Myc-DDK-tagged)-Human bestrophin 2 (BEST2)

Product Images

Western blot validation of overexpression lysate (Cat# LY402608) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218758 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BEST2
• Synonyms: VMD2L1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218758 representing NM_017682
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCGTCACCTACACAGCCCGAGTGGCGAACGCCCGCTTCGGTGGCTTCTCCCAGCTGCTGCTACTGT
  GGCGTGGGAGCATCTACAAACTCCTGTGGCGAGAGCTGCTCTGCTTCCTTGGGTTCTACATGGCGCTGAG
  TGCTGCCTACCGCTTTGTGCTGACCGAAGGGCAGAAGCGCTACTTCGAGAAGCTTGTGATTTATTGTGAC
  CAGTATGCCAGCCTCATCCCTGTCTCCTTCGTGCTTGGCTTTTATGTGACGCTGGTGGTGAACCGCTGGT
  GGAGCCAGTACCTATGCATGCCGCTGCCCGACGCGCTCATGTGCGTGGTGGCGGGCACCGTGCACGGACG
  CGACGACCGCGGCCGCCTCTACCGGCGCACACTCATGCGCTACGCAGGGCTCTCGGCCGTGCTCATCCTG
  CGCTCCGTCAGCACCGCGGTGTTCAAGCGCTTCCCCACCATAGACCACGTGGTGGAGGCTGGGTTTATGA
  CCCGCGAGGAGCGCAAGAAGTTTGAAAACCTGAACTCATCCTACAACAAGTACTGGGTGCCCTGCGTCTG
  GTTCTCCAACCTGGCGGCACAGGCCCGACGCGAGGGCCGCATCCGCGACAACAGCGCCCTTAAGCTGCTG
  CTCGAGGAGCTGAATGTTTTTCGGGGCAAATGTGGAATGCTCTTTCACTATGACTGGATTAGCGTACCCC
  TCGTGTACACGCAGGTGGTGACCATCGCACTGTACAGCTACTTCCTGGCTTGCCTCATTGGTCGCCAGTT
  CCTGGACCCGGCTCAGGGTTACAAAGACCACGACCTAGACCTGTGTGTGCCCATCTTCACCCTCTTGCAG
  TTCTTCTTCTACGCCGGCTGGCTCAAGGTAGCTGAGCAGCTCATCAACCCCTTCGGAGAGGACGATGATG
  ACTTTGAGACCAACTTTCTGATCGATAGAAACTTCCAGGTGTCCATGCTGGCAGTGGACGAGATGTATGA
  TGACCTGGCTGTGCTGGAGAAGGACTTGTACTGGGATGCAGCCGAGGCTCGCGCCCCATACACAGCGGCT
  ACTGTCTTCCAGCTGCGGCAGCCTTCCTTCCAGGGCTCCACCTTTGACATCACGCTGGCCAAAGAAGACA
  TGCAGTTCCAGCGGCTGGACGGCTTGGATGGACCGATGGGAGAGGCGCCCGGCGACTTCCTGCAGCGCCT
  CCTGCCGGCGGGCGCGGGCATGGTCGCGGGAGGCCCGCTGGGCCGGCGCCTGTCCTTTCTACTCCGCAAG
  AACAGCTGCGTGTCGGAGGCGTCTACTGGGGCCAGCTGCTCATGCGCGGTTGTCCCCGAAGGCGCGGCCC
  CGGAGTGCAGCTGCGGGGACCCGCTGCTCGACCCCGGCCTGCCGGAGCCCGAGGCCCCGCCCCCTGCGGG
  TCCCGAACCGCTTACCCTCATCCCTGGGCCTGTCGAGCCCTTCAGCATCGTGACCATGCCCGGGCCCCGG
  GGTCCGGCGCCACCCTGGCTGCCCAGCCCTATTGGCGAGGAGGAGGAGAATCTGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218758 representing NM_017682
  Red=Cloning site Green=Tags(s)
  
  MTVTYTARVANARFGGFSQLLLLWRGSIYKLLWRELLCFLGFYMALSAAYRFVLTEGQKRYFEKLVIYCD
  QYASLIPVSFVLGFYVTLVVNRWWSQYLCMPLPDALMCVVAGTVHGRDDRGRLYRRTLMRYAGLSAVLIL
  RSVSTAVFKRFPTIDHVVEAGFMTREERKKFENLNSSYNKYWVPCVWFSNLAAQARREGRIRDNSALKLL
  LEELNVFRGKCGMLFHYDWISVPLVYTQVVTIALYSYFLACLIGRQFLDPAQGYKDHDLDLCVPIFTLLQ
  FFFYAGWLKVAEQLINPFGEDDDDFETNFLIDRNFQVSMLAVDEMYDDLAVLEKDLYWDAAEARAPYTAA
  TVFQLRQPSFQGSTFDITLAKEDMQFQRLDGLDGPMGEAPGDFLQRLLPAGAGMVAGGPLGRRLSFLLRK
  NSCVSEASTGASCSCAVVPEGAAPECSCGDPLLDPGLPEPEAPPPAGPEPLTLIPGPVEPFSIVTMPGPR
  GPAPPWLPSPIGEEEENLA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017682
• ORF Size: 1527 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017682.2, NP_060152.2
• RefSeq Size: 1924 bp
• RefSeq ORF: 1530 bp
• Locus ID: 54831
• Protein Families: Transmembrane
• MW: 57 kDa
• Gene Summary: This gene is a member of the bestrophin gene family of anion channels. Bestrophin genes share a similar gene structure with highly conserved exon-intron boundaries, but with distinct 3' ends. Bestrophins are transmembrane proteins that contain a homologous region rich in aromatic residues, including an invariant arg-phe-pro motif. Mutation in one of the family members (bestrophin 1) is associated with vitelliform macular dystrophy. The bestrophin 2 gene is mainly expressed in the retinal pigment epithelium and colon. [provided by RefSeq, Jul 2008]