ARHGEF7 (NM_145735) Human Tagged ORF Clone

CAT#: RC218798L4

Lenti ORF clone of Human Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 2, mGFP tagged

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: mGFP
• Symbol: ARHGEF7
• Synonyms: BETA-PIX; COOL-1; COOL1; Nbla10314; P50; P50BP; P85; P85COOL1; P85SPR; PAK3; PIXB
• Vector: pLenti-C-mGFP-P2A-Puro
• E. coli Selection: Chloramphenicol (34 ug/mL)
• Mammalian Cell Selection: Puromycin
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(RC218798).
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NM_145735
• ORF Size: 2346 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_145735.1, NP_663788.1
• RefSeq Size: 5304
• RefSeq ORF: 2349
• Locus ID: 8874
• Domains: RhoGEF, SH3, CH, PH
• Protein Pathways: Regulation of actin cytoskeleton
• MW: 87.6 kDa
• Gene Summary: This gene encodes a protein that belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It forms a complex with the small GTP binding protein Rac1 and recruits Rac1 to membrane ruffles and to focal adhesions. Multiple alternatively spliced transcript variants encoding different isoforms have been observed for this gene. [provided by RefSeq, Mar 2016]