APC2 (NM_005883) Human Tagged ORF Clone

CAT#: RC218996

APC2 (Myc-DDK-tagged)-Human adenomatosis polyposis coli 2 (APC2)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: APC2
• Synonyms: APCL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218996 representing NM_005883
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAGCTCCGTGGCGCCCTACGAGCAGCTGGTGAGGCAGGTGGAGGCCTTGAAGGCTGAGAACAGCC
  ACCTGAGGCAGGAGCTAAGGGACAACTCCAGCCACCTGTCCAAGCTGGAGACAGAGACGTCGGGCATGAA
  GGAGGTCCTGAAGCACCTACAGGGAAAACTGGAGCAGGAGGCCCGAGTGCTGGTGTCCTCGGGGCAGACG
  GAGGTGCTGGAGCAGCTGAAGGCCCTACAGATGGACATCACCAGCCTGTACAACCTCAAGTTCCAGCCGC
  CCACCCTGGGCCCGGAGCCTGCCGCCCGGACCCCCGAGGGCAGCCCAGTACACGGCTCCGGGCCCTCCAA
  GGACAGCTTTGGGGAGCTGAGCCGGGCCACCATCCGGCTGCTGGAGGAACTGGACCGGGAACGGTGTTTC
  CTGCTGAATGAGATTGAGAAGGAGGAGAAGGAGAAGCTCTGGTACTACTCTCAGCTGCAGGGCCTGTCCA
  AGCGCCTGGACGAGCTGCCGCACGTGGAGACGCAGTTCTCGATGCAGATGGACCTGATCCGGCAGCAGCT
  TGAGTTCGAGGCCCAGCACATCCGCTCGCTGATGGAGGAGCGCTTCGGCACCTCGGACGAGATGGTGCAG
  CGGGCACAGATCCGCGCCTCGCGCCTGGAGCAGATTGACAAGGAGCTGCTGGAGGCGCAGGACCGAGTGC
  AGCAGACGGAGCCCCAGGCCTTGCTGGCGGTGAAGTCGGTGCCGGTGGACGAGGACCCCGAGACAGAGGT
  CCCCACACACCCTGAGGATGGCACCCCTCAGCCGGGCAACAGCAAGGTGGAGGTGGTCTTCTGGCTGTTG
  TCCATGTTGGCGACGCGCGACCAGGAGGATACAGCGCGCACGCTGCTGGCCATGTCCAGCTCGCCCGAGA
  GCTGCGTGGCCATGCGCCGCTCGGGCTGTCTGCCTCTGCTGCTGCAAATCCTCCACGGCACCGAGGCCGC
  GGCCGGGGGTCGCGCCGGGGCCCCAGGGGCACCGGGCGCCAAGGACGCACGCATGCGCGCCAACGCGGCG
  CTGCACAACATCGTCTTCTCGCAGCCGGACCAGGGCCTGGCGCGCAAGGAGATGCGCGTCCTGCACGTGC
  TGGAGCAGATCCGGGCCTACTGCGAGACCTGCTGGGACTGGCTGCAGGCCCGAGACGGCGGGCCCGAGGG
  AGGTGGCGCCGGCAGCGCCCCGATCCCCATCGAGCCGCAGATCTGCCAGGCCACCTGTGCTGTTATGAAG
  CTGTCCTTTGATGAGGAGTACCGCCGTGCCATGAACGAGCTAGGTGGGCTGCAGGCCGTGGCAGAGCTGC
  TGCAGGTTGACTATGAGATGCACAAGATGACCCGGGACCCGCTGAACCTGGCGCTGCGCCGCTACGCGGG
  CATGACCCTCACCAACCTCACCTTTGGGGACGTTGCCAACAAGGCCACCCTGTGTGCGCGCCGCGGCTGC
  ATGGAGGCCATCGTGGCCCAGCTGGCCTCCGACAGTGAGGAGCTCCACCAGGTGGTGTCCAGCATCCTTC
  GGAACTTGTCCTGGAGGGCCGACATCAACAGCAAGAAGGTGCTGAGGGAGGCGGGCAGCGTGACTGCCCT
  GGTGCAGTGTGTCCTGCGGGCCACCAAGGAGTCCACCCTGAAGAGCGTGCTGAGCGCCCTGTGGAATCTG
  TCTGCACACAGCACAGAGAACAAGGCGGCCATCTGCCAGGTGGATGGCGCCCTGGGCTTCCTGGTGAGCA
  CCCTGACCTACAAGTGTCAGAGCAACTCGCTGGCCATCATCGAGAGCGGCGGCGGCATCCTCCGCAATGT
  GTCCAGCCTCGTCGCCACCCGTGAGGACTACAGGCAGGTGCTCCGGGATCACAACTGTCTGCAGACGCTG
  CTGCAGCATCTGACTTCGCACAGCCTGACCATCGTGAGCAACGCGTGCGGCACGCTCTGGAACCTGTCGG
  CCCGCAGCGCCCGTGACCAGGAGCTGCTGTGGGACCTGGGCGCCGTGGGCATGCTGCGTAATCTGGTGCA
  CTCCAAGCACAAGATGATCGCCATGGGCAGCGCCGCCGCCCTGCGCAACCTGCTGGCCCATCGGCCCGCC
  AAGCACCAGGCGGCCGCCACCGCCGTGTCCCCAGGCAGCTGCGTGCCCAGCCTGTACGTGCGCAAGCAGC
  GGGCGCTGGAGGCCGAGCTGGACGCACGGCACCTCGCGCAGGCGCTGGAGCACCTGGAGAAGCAGGGCCC
  GCCGGCAGCCGAGGCCGCCACTAAGAAGCCGCTGCCGCCCCTGCGACACCTGGACGGCCTGGCCCAAGAC
  TATGCTTCCGATTCGGGCTGCTTTGACGACGACGATGCACCGTCATCCCTGGCTGCGGCCGCGGCCACCG
  GGGAGCCAGCCAGCCCTGCCGCGCTGTCCCTCTTCCTGGGCAGCCCCTTCCTGCAGGGGCAGGCGCTGGC
  TCGCACCCCGCCCACCCGCCGAGGCGGCAAGGAGGCAGAGAAGGACACCAGTGGGGAGGCAGCCGTGGCG
  GCCAAGGCCAAGGCCAAGCTGGCGCTTGCAGTGGCGCGCATCGACCAGCTGGTGGAGGACATCTCCGCCC
  TGCACACCTCGTCCGACGATAGCTTCAGCCTCAGCTCTGGAGACCCGGGACAGGAGGCGCCACGGGAGGG
  CCGCGCCCAGTCCTGCTCGCCATGCCGCGGCCCGGAGGGCGGGCGGCGAGAGGCAGGAAGCCGGGCGCAC
  CCGCTGCTGCGGCTCAAGGCGGCCCACGCCAGCCTCTCCAACGACAGCCTCAACAGCGGCAGTGCCAGCG
  ACGGGTACTGCCCACGCGAACATATGCTGCCCTGCCCGCTGGCCGCACTGGCTTCGCGCCGCGAGGACCC
  CAGGTGTGGGCAGCCTCGGCCCAGCCGGCTTGACCTTGACCTGCCCGGCTGCCAGGCCGAGCCCCCGGCC
  CGCGAGGCCACCTCCGCCGACGCCCGCGTGCGCACCATCAAGCTGTCGCCTACCTATCAGCACGTGCCAC
  TGCTTGAGGGTGCCTCAAGGGCGGGTGCAGAGCCCCTCGCGGGGCCTGGAATCTCTCCAGGGGCCCGGAA
  GCAGGCCTGGCTGCCGGCAGACCACCTGAGCAAGGTTCCCGAGAAGCTGGCGGCTGCCCCGCTGTCTGTG
  GCCAGCAAGGCACTGCAGAAACTGGCGGCGCAAGAGGGGCCACTCTCGCTGTCCCGATGCAGCTCCCTTT
  CCTCGCTGTCCTCGGCCGGCCGCCCAGGCCCCAGCGAGGGTGGTGACCTGGATGACAGTGACTCCTCCCT
  GGAGGGGCTGGAGGAGGCCGGCCCCAGCGAGGCTGAGCTGGACAGCACGTGGCGGGCGCCCGGGGCCACC
  TCGCTGCCCGTAGCCATTCCGGCTCCCCGGCGTAACCGAGGCCGGGGCCTGGGGGTGGAAGACGCCACGC
  CGTCCAGCTCGTCGGAGAACTACGTGCAGGAGACACCGCTTGTGCTGAGCCGCTGCAGCTCTGTGAGCTC
  GCTGGGCAGCTTCGAGAGCCCGTCCATCGCCAGCTCCATCCCCAGTGAACCTTGCAGCGGGCAGGGCAGC
  GGCACCATCAGCCCTAGCGAGCTGCCCGACAGCCCCGGACAGACCATGCCTCCCAGCCGGAGCAAGACGC
  CACCGCTGGCGCCCGCGCCACAGGGTCCCCCCGAGGCCACCCAGTTCAGCCTGCAGTGGGAGAGCTACGT
  GAAGCGCTTCCTGGACATCGCCGACTGCCGGGAGCGCTGCCGGCTGCCATCTGAGCTGGACGCAGGCAGC
  GTGCGCTTTACCGTGGAGAAGCCAGACGAGAACTTCTCGTGCGCCTCCAGCCTCAGCGCGCTGGCCTTGC
  ACGAGCACTACGTGCAGCAGGACGTGGAGCTGCGGCTGCTGCCCTCGGCCTGCCCCGAGCGCGGCGGGGG
  CGCCGGGGGCGCCGGCCTCCACTTTGCAGGGCACCGGCGGCGGGAGGAGGGGCCGGCGCCCACGGGTTCT
  CGCCCTCGCGGCGCCGCGGACCAGGAGCTGGAACTGCTGCGGGAGTGCCTGGGAGCCGCCGTGCCTGCCC
  GGCTGCGCAAGGTGGCCTCCGCGCTGGTGCCAGGTCGCCGCGCACTCCCCGTGCCCGTCTACATGTTGGT
  GCCCGCCCCGGCCCCGGCCCAGGAGGACGACTCCTGCACTGACTCCGCGGAGGGCACGCCGGTCAACTTC
  TCTAGCGCCGCCTCGCTCAGCGACGAGACGCTGCAGGGACCCCCCAGGGACCAGCCCGGGGGACCAGCGG
  GCAGGCAAAGACCCACCGGCCGCCCCACCTCTGCCAGACAGGCCATGGGGCACCGGCACAAGGCGGGAGG
  CGCCGGCCGCAGCGCGGAGCAGTCTCGGGGCGCGGGCAAGAACAGAGCAGGGCTGGAGCTGCCCCTGGGC
  CGGCCCCCGAGCGCCCCCGCAGACAAGGACGGCTCAAAGCCCGGCCGGACCCGCGGGGACGGGGCGCTCC
  AGTCGCTGTGCCTCACGACGCCCACTGAGGAGGCCGTGTACTGCTTCTACGGCAACGACTCGGACGAGGA
  GCCCCCGGCGGCCGCGCCCACGCCAACCCACCGGCGCACATCGGCCATCCCTCGCGCTTTTACGCGGGAG
  CGTCCGCAGGGCCGGAAGGAGGCCCCTGCCCCGTCCAAGGCTGCACCAGCTGCCCCGCCGCCCGCCCGGA
  CCCAGCCCAGCCTCATTGCTGACGAGACCCCGCCCTGCTACTCCCTGAGCTCCTCCGCCAGCTCCCTCAG
  CGAGCCCGAGCCCTCGGAGCCGCCGGCCGTCCATCCACGAGGCCGGGAGCCCGCGGTCACCAAGGACCCG
  GGCCCAGGAGGCGGACGCGACAGCTCGCCCAGCCCGCGGGCCGCGGAGGAGCTTCTGCAGCGGTGCATCA
  GCTCGGCCCTGCCCAGGCGCCGGCCCCCCGTGTCTGGCCTGCGGCGCCGCAAGCCCCGAGCCACCCGGCT
  GGATGAGCGGCCCGCAGAGGGGTCCCGGGAACGCGGCGAGGAGGCAGCGGGCTCGGACCGGGCCTCCGAC
  CTGGATAGCGTGGAGTGGCGCGCCATCCAGGAGGGCGCCAATTCAATTGTCACGTGGCTGCACCAGGCAG
  CAGCTGCCACGCGGGAGGCCTCGTCCGAGTCCGACTCCATCCTGTCCTTCGTATCCGGGCTGTCAGTGGG
  ATCCACCCTACAGCCCCCCAAGCACAGGAAGGGACGACAGGCGGAGGGAGAAATGGGCAGTGCCCGGCGG
  CCAGAGAAAAGGGGCGCAGCCTCAGTCAAGACCAGCGGGAGCCCCCGTTCCCCTGCAGGCCCCGAGAAGC
  CACGTGGCACACAGAAGACCACGCCCGGGGTGCCAGCTGTGCTCCGGGGACGAACAGTGATCTACGTCCC
  CAGCCCGGCACCCCGTGCCCAGCCCAAAGGGACCCCCGGCCCCCGCGCCACACCGCGGAAGGTGGCGCCC
  CCTTGCCTGGCACAGCCCGCGGCTCCAGCCAAAGTCCCGAGCCCCGGGCAGCAGCGGTCGCGGAGCCTAC
  ACCGGCCTGCCAAGACCTCGGAGCTGGCGACGCTGAGCCAGCCCCCCAGAAGCGCCACACCGCCCGCCCG
  CCTCGCCAAGACCCCCTCCTCCAGCTCCTCCCAGACCTCGCCCGCCTCCCAGCCCCTGCCCAGAAAGCGC
  CCCCCGGTCACCCAGGCTGCTGGGGCCCTGCCCGGCCCCGGAGCCTCCCCGGTGCCCAAAACGCCGGCGC
  GCACCCTTCTGGCGAAGCAGCACAAGACGCAGAGATCGCCCGTGCGGATCCCGTTCATGCAGAGGCCGGC
  CCGGCGTGGGCCGCCACCGCTGGCTCGGGCAGTCCCGGAGCCGGGCCCCAGGGGCCGGGCGGGGACCGAG
  GCGGGCCCGGGGGCGCGCGGGGGCCGCCTGGGCCTGGTGCGTGTGGCCTCAGCCCTCTCCAGCGGCAGCG
  AGTCCTCCGACCGCTCGGGCTTCCGGCGACAGCTAACCTTCATCAAGGAGTCGCCGGGCTTGCGGCGCCG
  CCGCTCCGAGCTGTCCTCGGCCGAGTCCGCGGCCTCTGCCCCCCAGGGCGCCTCGCCCCGCCGCGGCCGG
  CCCGCGCTGCCCGCCGTCTTCCTCTGCTCCTCGCGCTGCGAAGAGCTCCGAGCGGCACCCCGGCAGGGCC
  CGGCCCCGGCCCGGCAGCGGCCCCCCGCGGCCCGACCCAGCCCTGGCGAGCGCCCTGCCCGGCGCACCAC
  CTCCGAGAGCCCGTCCCGCCTGCCTGTGCGCGCGCCCGCCGCCCGGCCGGAGACTGTCAAGCGCTACGCG
  TCGCTGCCGCACATCAGCGTGGCCCGCAGGCCCGACGGCGCCGTCCCCGCGGCCCCTGCCTCAGCCGACG
  CCGCGCGCCGCAGCAGCGACGGGGAGCCCCGGCCGCTCCCCAGGGTGGCCGCGCCGGGCACGACCTGGCG
  GCGCATCCGAGATGAGGACGTGCCCCACATCCTGCGCAGCACGCTTCCCGCCACGGCCCTGCCACTGCGG
  GGCTCCACGCCCGAGGACGCCCCGGCCGGGCCCCCGCCGCGCAAGACCAGCGACGCCGTGGTCCAGACCG
  AGGAGGTCGCCGCCCCCAAGACCAACTCCAGCACGTCCCCGAGCCTGGAGACCAGGGAGCCCCCCGGGGC
  CCCCGCCGGCGGCCAGCTCTCCCTCCTCGGCAGCGACGTGGACGGTCCCAGCCTCGCCAAGGCTCCCATC
  TCCGCACCCTTCGTGCACGAGGGCCTGGGGGTCGCCGTGGGGGGCTTCCCCGCCAGCCGGCACGGCTCCC
  CCAGCCGCTCGGCCCGAGTACCCCCCTTCAACTATGTGCCCAGCCCCATGGTGGTCGCAGCCACCACCGA
  CTCGGCCGCGGAGAAAGCCCCGGCCACTGCCTCCGCCACCCTCCTGGAA
  
  CTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAGGATGACGACG
  ATAAGGTTTAA
  Protein Sequence

  >RC218996 representing NM_005883
  Red=Cloning site Green=Tags(s)
  
  MASSVAPYEQLVRQVEALKAENSHLRQELRDNSSHLSKLETETSGMKEVLKHLQGKLEQEARVLVSSGQT
  EVLEQLKALQMDITSLYNLKFQPPTLGPEPAARTPEGSPVHGSGPSKDSFGELSRATIRLLEELDRERCF
  LLNEIEKEEKEKLWYYSQLQGLSKRLDELPHVETQFSMQMDLIRQQLEFEAQHIRSLMEERFGTSDEMVQ
  RAQIRASRLEQIDKELLEAQDRVQQTEPQALLAVKSVPVDEDPETEVPTHPEDGTPQPGNSKVEVVFWLL
  SMLATRDQEDTARTLLAMSSSPESCVAMRRSGCLPLLLQILHGTEAAAGGRAGAPGAPGAKDARMRANAA
  LHNIVFSQPDQGLARKEMRVLHVLEQIRAYCETCWDWLQARDGGPEGGGAGSAPIPIEPQICQATCAVMK
  LSFDEEYRRAMNELGGLQAVAELLQVDYEMHKMTRDPLNLALRRYAGMTLTNLTFGDVANKATLCARRGC
  MEAIVAQLASDSEELHQVVSSILRNLSWRADINSKKVLREAGSVTALVQCVLRATKESTLKSVLSALWNL
  SAHSTENKAAICQVDGALGFLVSTLTYKCQSNSLAIIESGGGILRNVSSLVATREDYRQVLRDHNCLQTL
  LQHLTSHSLTIVSNACGTLWNLSARSARDQELLWDLGAVGMLRNLVHSKHKMIAMGSAAALRNLLAHRPA
  KHQAAATAVSPGSCVPSLYVRKQRALEAELDARHLAQALEHLEKQGPPAAEAATKKPLPPLRHLDGLAQD
  YASDSGCFDDDDAPSSLAAAAATGEPASPAALSLFLGSPFLQGQALARTPPTRRGGKEAEKDTSGEAAVA
  AKAKAKLALAVARIDQLVEDISALHTSSDDSFSLSSGDPGQEAPREGRAQSCSPCRGPEGGRREAGSRAH
  PLLRLKAAHASLSNDSLNSGSASDGYCPREHMLPCPLAALASRREDPRCGQPRPSRLDLDLPGCQAEPPA
  REATSADARVRTIKLSPTYQHVPLLEGASRAGAEPLAGPGISPGARKQAWLPADHLSKVPEKLAAAPLSV
  ASKALQKLAAQEGPLSLSRCSSLSSLSSAGRPGPSEGGDLDDSDSSLEGLEEAGPSEAELDSTWRAPGAT
  SLPVAIPAPRRNRGRGLGVEDATPSSSSENYVQETPLVLSRCSSVSSLGSFESPSIASSIPSEPCSGQGS
  GTISPSELPDSPGQTMPPSRSKTPPLAPAPQGPPEATQFSLQWESYVKRFLDIADCRERCRLPSELDAGS
  VRFTVEKPDENFSCASSLSALALHEHYVQQDVELRLLPSACPERGGGAGGAGLHFAGHRRREEGPAPTGS
  RPRGAADQELELLRECLGAAVPARLRKVASALVPGRRALPVPVYMLVPAPAPAQEDDSCTDSAEGTPVNF
  SSAASLSDETLQGPPRDQPGGPAGRQRPTGRPTSARQAMGHRHKAGGAGRSAEQSRGAGKNRAGLELPLG
  RPPSAPADKDGSKPGRTRGDGALQSLCLTTPTEEAVYCFYGNDSDEEPPAAAPTPTHRRTSAIPRAFTRE
  RPQGRKEAPAPSKAAPAAPPPARTQPSLIADETPPCYSLSSSASSLSEPEPSEPPAVHPRGREPAVTKDP
  GPGGGRDSSPSPRAAEELLQRCISSALPRRRPPVSGLRRRKPRATRLDERPAEGSRERGEEAAGSDRASD
  LDSVEWRAIQEGANSIVTWLHQAAAATREASSESDSILSFVSGLSVGSTLQPPKHRKGRQAEGEMGSARR
  PEKRGAASVKTSGSPRSPAGPEKPRGTQKTTPGVPAVLRGRTVIYVPSPAPRAQPKGTPGPRATPRKVAP
  PCLAQPAAPAKVPSPGQQRSRSLHRPAKTSELATLSQPPRSATPPARLAKTPSSSSSQTSPASQPLPRKR
  PPVTQAAGALPGPGASPVPKTPARTLLAKQHKTQRSPVRIPFMQRPARRGPPPLARAVPEPGPRGRAGTE
  AGPGARGGRLGLVRVASALSSGSESSDRSGFRRQLTFIKESPGLRRRRSELSSAESAASAPQGASPRRGR
  PALPAVFLCSSRCEELRAAPRQGPAPARQRPPAARPSPGERPARRTTSESPSRLPVRAPAARPETVKRYA
  SLPHISVARRPDGAVPAAPASADAARRSSDGEPRPLPRVAAPGTTWRRIRDEDVPHILRSTLPATALPLR
  GSTPEDAPAGPPPRKTSDAVVQTEEVAAPKTNSSTSPSLETREPPGAPAGGQLSLLGSDVDGPSLAKAPI
  SAPFVHEGLGVAVGGFPASRHGSPSRSARVPPFNYVPSPMVVAATTDSAAEKAPATASATLLE
  
  LEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-XhoI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005883
• ORF Size: 6909 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_005883.1, NM_005883.2, NP_005874.1
• RefSeq Size: 10151
• RefSeq ORF: 6912
• Locus ID: 10297
• Domains: Armadillo_seg
• Protein Families: Druggable Genome
• Protein Pathways: Basal cell carcinoma, Colorectal cancer, Endometrial cancer, Pathways in cancer, Regulation of actin cytoskeleton, Wnt signaling pathway
• MW: 243.8 kDa
• Gene Summary: This gene encodes a strongly conserved protein that has an N-terminal coiled-coil domain followed by an armadillo domain, five 20-amino acid repeats, and two SAMP domains. This protein promotes the assembly of a multiprotein complex that recruits and phosphorylates the Wnt effector beta-catenin and targets beta-catenin for ubiquitylation and proteasomal degradation. This protein therefore plays a role in the reduction of cytoplasmic levels of beta-catenin which in turn reduces activation of Wnt target genes that play a pivotal role in the pathogenesis of various human cancers. The protein encoded by this gene is closely related to the adenomatous polyposis coli (APC) tumor-suppressor protein and has similar tumor-suppressor effects. This gene also plays a role in actin assembly, cell-cell adhesion, and microtubule network formation through its interaction with cytoskeletal proteins. This gene has its highest expression in the central nervous system and is involved in brain development through cytoskeletal regulation in neurons. Alternative splicing produces multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2017]