IKB zeta (NFKBIZ) (NM_031419) Human Tagged ORF Clone

CAT#: RC219121

NFKBIZ (Myc-DDK-tagged)-Human nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, zeta (NFKBIZ), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY410530) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219121 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NFKBIZ
• Synonyms: IKBZ; INAP; MAIL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219121 representing NM_031419
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTGTGGACAAGCTGCTGGACGACAGCCGCGGCGGAGAGGGGCTGCGGGACGCGGCGGGCGGCTGCG
  GCCTCATGACCAGCCCGCTCAACCTGAGCTACTTCTACGGCGCGTCGCCGCCCGCCGCCGCCCCGGGCGC
  CTGCGACGCCAGCTGCTCGGTCTTGGGCCCCTCGGCGCCCGGCTCGCCCGGCTCCGACTCCTCCGACTTC
  TCCTCTGCCTCGTCGGTGTCCTCCTGCGGCGCCGTGGAGTCCCGGTCGAGAGGCGGCGCCCGCGCCGAGC
  GCCAGCCAGTTGAGCCCCATATGGGGGTTGGCAGGCAGCAGAGAGGCCCCTTTCAAGGTGTTCGGGTAAA
  GAACTCAGTGAAGGAACTCCTGTTGCACATCCGAAGTCATAAACAGAAGGCTTCTGGCCAAGCTGTGGAT
  GATTTTAAGACACAAGGTGTGAACATAGAACAGTTCAGAGAATTGAAGAACACAGTATCATACAGTGGGA
  AAAGGAAAGGGCCCGATTCGTTGTCTGATGGACCTGCTTGCAAAAGGCCAGCTCTGTTGCATTCCCAATT
  TTTGACACCACCTCAAACACCAACGCCCGGGGAGAGCATGGAAGATGTTCATCTCAATGAACCCAAACAG
  GAGAGCAGTGCTGATCTGCTTCAGAACATTATCAACATTAAGAATGAATGCAGCCCCGTTTCCCTGAACA
  CAGTTCAAGTTAGCTGGCTGAACCCCGTGGTGGTCCCTCAGAGCTCCCCCGCAGAGCAGTGTCAGGACTT
  CCATGGAGGGCAGGTCTTTTCTCCACCTCAGAAATGCCAACCATTCCAAGTCAGGGGCTCCCAACAAATG
  ATAGACCAGGCTTCCCTGTACCAGTATTCTCCACAGAACCAGCATGTAGAGCAGCAGCCACACTACACCC
  ACAAACCAACTCTGGAATACAGTCCTTTTCCCATACCTCCCCAGTCCCCCGCTTATGAACCAAACCTCTT
  TGATGGTCCAGAATCACAGTTTTGCCCAAACCAAAGCTTAGTTTCCCTTCTTGGTGATCAAAGGGAATCT
  GAGAATATTGCTAATCCCATGCAGACTTCCTCCAGTGTTCAGCAGCAAAATGATGCTCACTTGCACAGCT
  TCAGCATGATGCCCAGCAGCGCCTGTGAGGCCATGGTGGGGCACGAGATGGCCTCTGACTCTTCAAACAC
  TTCACTGCCATTCTCAAACATGGGAAATCCAATGAACACCACACAGTTAGGGAAATCACTTTTTCAGTGG
  CAGGTGGAGCAGGAAGAAAGCAAATTGGCAAATATTTCCCAAGACCAGTTTCTTTCAAAGGATGCAGATG
  GTGACACGTTCCTTCATATTGCTGTTGCCCAAGGGAGAAGGGCACTTTCCTATGTTCTTGCAAGAAAGAT
  GAATGCACTTCACATGCTGGATATTAAAGAGCACAATGGACAGAGTGCCTTTCAGGTGGCAGTGGCTGCC
  AATCAGCATCTCATTGTGCAGGATCTGGTGAACATCGGGGCACAGGTGAACACCACAGACTGCTGGGGAA
  GAACACCTCTGCATGTGTGTGCTGAGAAGGGCCACTCCCAGGTGCTTCAGGCGATTCAGAAGGGAGCAGT
  GGGAAGTAATCAGTTTGTGGATCTTGAGGCAACTAACTATGATGGCCTGACTCCCCTTCACTGTGCAGTC
  ATAGCCCACAATGCTGTGGTCCATGAACTCCAGAGAAATCAACAGCCTCATTCACCTGAAGTTCAGGAGC
  TTTTACTGAAGAATAAGAGTCTGGTTGATACCATTAAGTGCCTAATTCAAATGGGAGCAGCGGTGGAAGC
  GAAGGATCGCAAAAGTGGCCGCACAGCCCTGCATTTGGCAGCTGAAGAAGCAAATCTGGAACTCATTCGC
  CTCTTTTTGGAGCTGCCCAGTTGCCTGTCTTTTGTGAATGCAAAGGCTTACAATGGCAACACTGCCCTCC
  ATGTTGCTGCCAGCTTGCAGTATCGGTTGACACAATTAGATGCTGTCCGCCTGTTGATGAGGAAGGGAGC
  AGACCCAAGTACTCGGAACTTGGAGAACGAACAGCCAGTGCATTTGGTTCCCGATGGCCCTGTGGGAGAA
  CAGATCCGACGTATCCTGAAGGGAAAGTCCATTCAGCAGAGAGCTCCACCGTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219121 representing NM_031419
  Red=Cloning site Green=Tags(s)
  
  MIVDKLLDDSRGGEGLRDAAGGCGLMTSPLNLSYFYGASPPAAAPGACDASCSVLGPSAPGSPGSDSSDF
  SSASSVSSCGAVESRSRGGARAERQPVEPHMGVGRQQRGPFQGVRVKNSVKELLLHIRSHKQKASGQAVD
  DFKTQGVNIEQFRELKNTVSYSGKRKGPDSLSDGPACKRPALLHSQFLTPPQTPTPGESMEDVHLNEPKQ
  ESSADLLQNIINIKNECSPVSLNTVQVSWLNPVVVPQSSPAEQCQDFHGGQVFSPPQKCQPFQVRGSQQM
  IDQASLYQYSPQNQHVEQQPHYTHKPTLEYSPFPIPPQSPAYEPNLFDGPESQFCPNQSLVSLLGDQRES
  ENIANPMQTSSSVQQQNDAHLHSFSMMPSSACEAMVGHEMASDSSNTSLPFSNMGNPMNTTQLGKSLFQW
  QVEQEESKLANISQDQFLSKDADGDTFLHIAVAQGRRALSYVLARKMNALHMLDIKEHNGQSAFQVAVAA
  NQHLIVQDLVNIGAQVNTTDCWGRTPLHVCAEKGHSQVLQAIQKGAVGSNQFVDLEATNYDGLTPLHCAV
  IAHNAVVHELQRNQQPHSPEVQELLLKNKSLVDTIKCLIQMGAAVEAKDRKSGRTALHLAAEEANLELIR
  LFLELPSCLSFVNAKAYNGNTALHVAASLQYRLTQLDAVRLLMRKGADPSTRNLENEQPVHLVPDGPVGE
  QIRRILKGKSIQQRAPPY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031419
• ORF Size: 2154 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031419.1, NM_031419.2, NM_031419.3, NP_113607.1
• RefSeq Size: 3934
• RefSeq ORF: 2157
• Locus ID: 64332
• Domains: ANK
• Protein Families: Druggable Genome
• MW: 78.5 kDa
• Gene Summary: This gene is a member of the ankyrin-repeat family and is induced by lipopolysaccharide (LPS). The C-terminal portion of the encoded product which contains the ankyrin repeats, shares high sequence similarity with the I kappa B family of proteins. The latter are known to play a role in inflammatory responses to LPS by their interaction with NF-B proteins through ankyrin-repeat domains. Studies in mouse indicate that this gene product is one of the nuclear I kappa B proteins and an activator of IL-6 production. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]