CACNA1I (NM_001003406) Human Tagged ORF Clone

CAT#: RC219238

CACNA1I (Myc-DDK-tagged)-Human calcium channel, voltage-dependent, T type, alpha 1I subunit (CACNA1I), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CACNA1I
• Synonyms: ca(v)3.3; Cav3.3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219238 representing NM_001003406
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGAGAGCGCCTCCCCGCCCTCCTCATCTGCAGCAGCCCCAGCCGCTGAGCCAGGAGTCACCACGG
  AGCAGCCCGGACCCCGGAGCCCCCCATCCTCCCCGCCAGGCCTGGAGGAGCCTCTGGATGGAGCTGATCC
  TCATGTCCCACACCCAGACCTGGCGCCTATTGCCTTCTTCTGCCTGCGACAGACCACCAGCCCCCGGAAC
  TGGTGCATCAAGATGGTGTGCAACCCGTGGTTTGAATGTGTCAGCATGCTGGTGATCCTGCTGAACTGCG
  TGACACTTGGCATGTACCAGCCGTGCGACGACATGGACTGCCTGTCCGACCGCTGCAAGATCCTGCAGGT
  CTTTGATGACTTCATCTTTATCTTCTTTGCCATGGAGATGGTGCTCAAGATGGTGGCCCTGGGGATTTTT
  GGCAAGAAGTGCTACCTCGGGGACACATGGAACCGCCTGGATTTCTTCATCGTCATGGCAGGGATGGTCG
  AGTACTCCCTGGACCTTCAGAACATCAACCTGTCAGCCATCCGCACCGTGCGCGTCCTGAGGCCCCTCAA
  AGCCATCAACCGCGTGCCCAGTATGCGGATCCTGGTGAACCTGCTCCTGGACACACTGCCCATGCTGGGG
  AATGTCCTGCTGCTCTGCTTCTTTGTCTTCTTCATCTTTGGCATCATAGGTGTGCAGCTCTGGGCGGGCC
  TGCTGCGTAACCGCTGCTTCCTGGAGGAGAACTTCACCATACAAGGGGATGTGGCCTTGCCCCCATACTA
  CCAGCCGGAGGAGGATGATGAGATGCCCTTCATCTGCTCCCTGTCGGGCGACAATGGGATAATGGGCTGC
  CATGAGATCCCCCCGCTCAAGGAGCAGGGCCGTGAGTGCTGCCTGTCCAAGGACGACGTCTACGACTTTG
  GGGCGGGGCGCCAGGACCTCAATGCCAGCGGCCTCTGTGTCAACTGGAACCGTTACTACAATGTGTGCCG
  CACGGGCAGCGCCAACCCCCACAAGGGTGCCATCAACTTTGACAACATCGGTTATGCTTGGATTGTCATC
  TTCCAGGTGATCACTCTGGAAGGCTGGGTGGAGATCATGTACTACGTGATGGATGCTCACTCCTTCTACA
  ACTTCATCTACTTCATCCTGCTTATCATAGTGGGCTCCTTCTTCATGATCAACCTGTGCCTCGTTGTCAT
  AGCGACCCAGTTCTCGGAGACCAAGCAACGGGAGCACCGGCTGATGCTGGAGCAGCGGCAGCGCTACCTG
  TCCTCCAGCACGGTGGCCAGCTACGCCGAGCCTGGCGACTGCTACGAGGAGATCTTCCAGTATGTCTGCC
  ACATCCTGCGCAAGGCCAAGCGCCGCGCCCTGGGCCTCTACCAGGCCCTGCAGAGCCGGCGCCAGGCCCT
  GGGCCCGGAGGCCCCGGCCCCCGCCAAACCTGGGCCCCACGCCAAGGAGCCCCGGCACTACCAGCTGTGC
  CCGCAACATAGCCCCCTGGATGCGACGCCCCACACCCTGGTGCAGCCCATCCCCGCCACGCTGGCTTCCG
  ATCCCGCCAGCTGCCCTTGCTGCCAGCATGAGGACGGCCGGCGGCCCTCGGGCCTGGGCAGCACCGACTC
  GGGCCAGGAGGGCTCGGGCTCCGGGAGCTCCGCTGGTGGCGAGGACGAGGCGGATGGGGACGGGGCCCGG
  AGCAGCGAGGACGGAGCCTCCTCAGAACTGGGGAAGGAGGAGGAGGAGGAGGAGCAGGCGGATGGGGCGG
  TCTGGCTGTGCGGGGATGTGTGGCGGGAGACGCGAGCCAAGCTGCGCGGCATCGTGGACAGCAAGTACTT
  CAACCGGGGCATCATGATGGCCATCCTGGTCAACACCGTCAGCATGGGCATCGAGCACCACGAGCAGCCG
  GAGGAGCTGACCAACATCCTGGAGATCTGCAATGTGGTCTTCACCAGCATGTTTGCCCTGGAGATGATCC
  TGAAGCTGGCTGCATTTGGGCTCTTCGACTACCTGCGTAACCCCTACAACATCTTCGACAGCATCATTGT
  CATCATCAGCATCTGGGAGATTGTGGGGCAGGCGGACGGTGGGCTGTCGGTGCTGCGGACCTTCCGGCTG
  CTGCGCGTGCTGAAACTGGTGCGCTTCATGCCTGCCCTGCGGCGCCAGCTCGTGGTGCTCATGAAGACCA
  TGGACAACGTGGCCACCTTCTGCATGCTGCTCATGCTCTTCATCTTCATCTTCAGCATCCTTGGGATGCA
  TATTTTTGGCTGCAAGTTCAGCCTCCGCACGGACACTGGAGACACGGTGCCCGACAGGAAGAACTTCGAC
  TCCCTGCTGTGGGCCATCGTCACTGTGTTCCAGATCCTCACCCAGGAGGACTGGAACGTCGTTCTCTACA
  ATGGCATGGCCTCCACTTCTCCCTGGGCCTCCCTCTACTTTGTCGCCCTCATGACCTTCGGCAACTATGT
  GCTCTTCAACCTGCTGGTGGCCATCCTGGTGGAGGGCTTCCAGGCGGAGGGTGACGCCAATCGCTCCTAC
  TCGGACGAGGACCAGAGCTCATCCAACATAGAAGAGTTTGATAAGCTCCAGGAAGGCCTGGACAGCAGCG
  GAGATCCCAAGCTCTGCCCAATCCCCATGACCCCCAATGGGCACCTGGACCCCAGTCTCCCACTGGGTGG
  GCACCTAGGTCCTGCTGGGGCTGCGGGACCTGCCCCCCGACTCTCACTGCAGCCGGACCCCATGCTGGTG
  GCCCTGGGCTCCCGAAAGAGCAGTGTCATGTCTCTAGGGAGGATGAGCTATGACCAGCGCTCCCTGTCCA
  GCTCCCGGAGCTCCTACTACGGGCCATGGGGCCGCAGCGCGGCCTGGGCCAGCCGTCGCTCCAGCTGGAA
  CAGCCTCAAGCACAAGCCGCCGTCGGCGGAGCATGAGTCCCTGCTCTCTGCGGAGCGCGGCGGCGGCGCC
  CGGGTCTGCGAGGTTGCCGCGGACGAGGGGCCGCCGCGGGCCGCACCCCTGCACACCCCACACGCCCACC
  ACATTCATCACGGGCCCCATCTGGCGCACCGCCACCGCCACCACCGCCGGACGCTGTCCCTCGACAACAG
  GGACTCGGTGGACCTGGCCGAGCTGGTGCCCGCGGTGGGCGCCCACCCCCGGGCCGCCTGGAGGGCGGCA
  GGCCCGGCCCCCGGGCATGAGGACTGCAATGGCAGGATGCCCAGCATCGCCAAAGACGTCTTCACCAAGA
  TGGGCGACCGCGGGGATCGCGGGGAGGATGAGGAGGAAATCGACTACACCCTGTGCTTCCGCGTCCGCAA
  GATGATCGACGTCTATAAGCCCGACTGGTGCGAGGTCCGCGAAGACTGGTCTGTCTACCTCTTCTCTCCC
  GAGAACAGGTTCCGGGTCCTGTGTCAGACCATTATTGCCCACAAACTCTTCGACTACGTCGTCCTGGCCT
  TCATCTTTCTCAACTGCATCACCATCGCCCTGGAGCGGCCTCAGATCGAGGCCGGCAGCACCGAACGCAT
  CTTTCTCACCGTGTCCAACTACATCTTCACGGCCATCTTCGTGGGCGAGATGACATTGAAGGTAGTCTCG
  CTGGGCCTGTACTTCGGCGAGCAGGCGTACCTACGCAGCAGCTGGAACGTGCTGGATGGCTTTCTTGTCT
  TCGTGTCCATCATCGACATCGTGGTGTCCCTGGCCTCAGCCGGGGGAGCCAAGATCTTGGGGGTCCTCCG
  AGTCTTGCGGCTCCTGCGCACCCTACGCCCCCTGCGTGTCATCAGCCGGGCGCCGGGCCTGAAGCTGGTG
  GTGGAGACACTCATCTCCTCCCTCAAGCCCATCGGCAACATCGTGCTCATCTGCTGTGCCTTCTTCATCA
  TCTTTGGCATCCTGGGAGTGCAGCTCTTCAAGGGCAAGTTCTACCACTGTCTGGGCGTGGACACCCGCAA
  CATCACCAACCGCTCGGACTGCATGGCCGCCAACTACCGCTGGGTCCATCACAAATACAACTTCGACAAC
  CTGGGCCAGGCTCTGATGTCCCTCTTTGTCCTGGCATCCAAGGATGGTTGGGTGAACATCATGTACAATG
  GACTGGATGCTGTTGCTGTGGACCAGCAGCCTGTGACCAACCACAACCCCTGGATGCTGCTGTACTTCAT
  CTCCTTCCTGCTCATCGTCAGCTTCTTTGTGCTCAACATGTTTGTGGGTGTCGTGGTGGAGAACTTCCAC
  AAGTGCCGGCAGCACCAGGAGGCTGAAGAGGCACGGCGGCGTGAGGAGAAGCGGCTGCGGCGCCTGGAGA
  AGAAGCGCCGGAAGGCCCAGCGGCTGCCCTACTATGCCACCTATTGTCACACCCGGCTGCTCATCCACTC
  CATGTGCACCAGCCACTACCTGGACATCTTCATCACCTTCATCATCTGCCTCAACGTGGTCACCATGTCC
  CTGGAGCACTACAATCAGCCCACGTCCCTGGAGACAGCCCTCAAGTACTGCAACTATATGTTCACCACTG
  TCTTTGTGCTGGAGGCTGTGCTGAAGCTGGTGGCATTTGGTCTGAGGCGCTTCTTCAAGGACCGATGGAA
  CCAGCTGGACCTGGCCATTGTGCTACTGTCAGTCATGGGCATCACCCTGGAGGAGATCGAGATCAATGCG
  GCCCTGCCCATCAATCCCACCATCATCCGCATCATGAGGGTTCTGCGCATTGCCCGAGTGCTGAAGCTGT
  TGAAGATGGCCACAGGAATGCGGGCCCTGCTGGACACGGTGGTGCAAGCTTTGCCCCAGGTGGGCAACCT
  GGGCCTCCTCTTCATGCTGCTCTTCTTCATCTATGCTGCTCTCGGGGTGGAGCTCTTTGGGAAGCTGGTC
  TGCAACGACGAGAACCCGTGCGAGGGCATGAGCCGGCATGCCACCTTCGAGAACTTCGGCATGGCCTTCC
  TCACACTCTTCCAGGTCTCCACGGGTGACAACTGGAACGGGATCATGAAGGACACGCTGCGGGACTGCAC
  CCACGACGAGCGCAGCTGCCTGAGCAGCCTGCAGTTTGTGTCGCCGCTGTACTTCGTGAGCTTCGTGCTC
  ACCGCGCAGTTCGTGCTCATCAACGTGGTGGTGGCTGTGCTCATGAAGCACCTGGACGACAGCAACAAGG
  AGGCGCAGGAGGACGCCGAGATGGATGCCGAGCTCGAGCTGGAGATGGCCCATGGCCTGGGCCCTGGCCC
  GAGGCTGCCTACCGGCTCCCCGGGCGCCCCTGGCCGAGGGCCGGGAGGGGCGGGCGGCGGGGGCGACACC
  GAGGGCGGCTTGTGCCGGCGCTGCTACTCGCCTGCCCAGGAGAACCTGTGGCTGGACAGCGTCTCTTTAA
  TCATCAAGGACTCCTTGGAGGGGGAGCTGACCATCATCGACAACCTGTCGGGCTCCATCTTCCACCACTA
  CTCCTCGCCTGCCGGCTGCAAGAAGTGTCACCACGACAAGCAAGAGGTGCAGCTGGCTGAGACGGAGGCC
  TTCTCCCTGAACTCAGACAGGTCCTCGTCCATCCTGCTGGGTGACGACCTGAGTCTCGAGGACCCCACAG
  CCTGCCCACCTGGCCGCAAAGACAGCAAGGGTGAGCTGGACCCACCTGAGCCCATGCGTGTGGGAGACCT
  GGGCGAATGCTTCTTCCCCTTGTCCTCTACGGCCGTCTCGCCGGATCCAGAGAACTTCCTGTGTGAGATG
  GAGGAGATCCCATTCAACCCTGTCCGGTCCTGGCTGAAACATGACAGCAGTCAAGCACCCCCAAGTCCCT
  TCTCCCCGGATGCCTCCAGCCCTCTCCTGCCCATGCCAGCCGAGTTCTTCCACCCTGCAGTGTCTGCCAG
  CCAGAAAGGCCCAGAAAAGGGCACTGGCACTGGAACCCTCCCCAAGATTGCGCTGCAGGGCTCCTGGGCA
  TCTCTGCGGTCACCAAGGGTCAACTGTACCCTCCTCCGGCAGGCCACCGGGAGCGACACGTCGCTGGACG
  CCAGCCCCAGCAGCTCCGCGGGCAGCCTGCAGACCACGCTCGAGGACAGCCTGACCCTGAGCGACAGCCC
  CCGGCGTGCCCTGGGGCCGCCCGCGCCTGCTCCAGGACCCCGGGCCGGCCTGTCCCCCGCCGCTCGCCGC
  CGCCTGAGCCTGCGCGGCCGGGGCCTCTTCAGCCTGCGGGGGCTGCGGGCGCATCAGCGCAGCCACAGCA
  GCGGGGGCTCCACCAGCCCGGGCTGCACCCACCACGACTCCATGGACCCCTCGGACGAGGAGGGCCGCGG
  TGGCGCGGGCGGCGGGGGCGCGGGCAGCGAGCACTCGGAGACCCTCAGCAGCCTCTCGCTCACCTCCCTC
  TTCTGCCCGCCGCCCCCGCCGCCAGCCCCCGGCCTCACGCCCGCCAGGAAGTTCAGCAGCACCAGCAGCC
  TGGCCGCCCCCGGCCGCCCCCACGCCGCCGCCCTGGCCCACGGCCTGGCCCGGAGCCCCTCGTGGGCCGC
  GGACCGCAGCAAGGACCCCCCCGGCCGGGCACCGCTGCCCATGGGCCTGGGCCCCTTGGCGCCCCCGCCG
  CAACCGCTCCCCGGAGAGCTGGAGCCGGGAGACGCCGCCAGCAAGAGGAAGAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219238 representing NM_001003406
  Red=Cloning site Green=Tags(s)
  
  MAESASPPSSSAAAPAAEPGVTTEQPGPRSPPSSPPGLEEPLDGADPHVPHPDLAPIAFFCLRQTTSPRN
  WCIKMVCNPWFECVSMLVILLNCVTLGMYQPCDDMDCLSDRCKILQVFDDFIFIFFAMEMVLKMVALGIF
  GKKCYLGDTWNRLDFFIVMAGMVEYSLDLQNINLSAIRTVRVLRPLKAINRVPSMRILVNLLLDTLPMLG
  NVLLLCFFVFFIFGIIGVQLWAGLLRNRCFLEENFTIQGDVALPPYYQPEEDDEMPFICSLSGDNGIMGC
  HEIPPLKEQGRECCLSKDDVYDFGAGRQDLNASGLCVNWNRYYNVCRTGSANPHKGAINFDNIGYAWIVI
  FQVITLEGWVEIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQREHRLMLEQRQRYL
  SSSTVASYAEPGDCYEEIFQYVCHILRKAKRRALGLYQALQSRRQALGPEAPAPAKPGPHAKEPRHYQLC
  PQHSPLDATPHTLVQPIPATLASDPASCPCCQHEDGRRPSGLGSTDSGQEGSGSGSSAGGEDEADGDGAR
  SSEDGASSELGKEEEEEEQADGAVWLCGDVWRETRAKLRGIVDSKYFNRGIMMAILVNTVSMGIEHHEQP
  EELTNILEICNVVFTSMFALEMILKLAAFGLFDYLRNPYNIFDSIIVIISIWEIVGQADGGLSVLRTFRL
  LRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKFSLRTDTGDTVPDRKNFD
  SLLWAIVTVFQILTQEDWNVVLYNGMASTSPWASLYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSY
  SDEDQSSSNIEEFDKLQEGLDSSGDPKLCPIPMTPNGHLDPSLPLGGHLGPAGAAGPAPRLSLQPDPMLV
  ALGSRKSSVMSLGRMSYDQRSLSSSRSSYYGPWGRSAAWASRRSSWNSLKHKPPSAEHESLLSAERGGGA
  RVCEVAADEGPPRAAPLHTPHAHHIHHGPHLAHRHRHHRRTLSLDNRDSVDLAELVPAVGAHPRAAWRAA
  GPAPGHEDCNGRMPSIAKDVFTKMGDRGDRGEDEEEIDYTLCFRVRKMIDVYKPDWCEVREDWSVYLFSP
  ENRFRVLCQTIIAHKLFDYVVLAFIFLNCITIALERPQIEAGSTERIFLTVSNYIFTAIFVGEMTLKVVS
  LGLYFGEQAYLRSSWNVLDGFLVFVSIIDIVVSLASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLV
  VETLISSLKPIGNIVLICCAFFIIFGILGVQLFKGKFYHCLGVDTRNITNRSDCMAANYRWVHHKYNFDN
  LGQALMSLFVLASKDGWVNIMYNGLDAVAVDQQPVTNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFH
  KCRQHQEAEEARRREEKRLRRLEKKRRKAQRLPYYATYCHTRLLIHSMCTSHYLDIFITFIICLNVVTMS
  LEHYNQPTSLETALKYCNYMFTTVFVLEAVLKLVAFGLRRFFKDRWNQLDLAIVLLSVMGITLEEIEINA
  ALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLV
  CNDENPCEGMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRDCTHDERSCLSSLQFVSPLYFVSFVL
  TAQFVLINVVVAVLMKHLDDSNKEAQEDAEMDAELELEMAHGLGPGPRLPTGSPGAPGRGPGGAGGGGDT
  EGGLCRRCYSPAQENLWLDSVSLIIKDSLEGELTIIDNLSGSIFHHYSSPAGCKKCHHDKQEVQLAETEA
  FSLNSDRSSSILLGDDLSLEDPTACPPGRKDSKGELDPPEPMRVGDLGECFFPLSSTAVSPDPENFLCEM
  EEIPFNPVRSWLKHDSSQAPPSPFSPDASSPLLPMPAEFFHPAVSASQKGPEKGTGTGTLPKIALQGSWA
  SLRSPRVNCTLLRQATGSDTSLDASPSSSAGSLQTTLEDSLTLSDSPRRALGPPAPAPGPRAGLSPAARR
  RLSLRGRGLFSLRGLRAHQRSHSSGGSTSPGCTHHDSMDPSDEEGRGGAGGGGAGSEHSETLSSLSLTSL
  FCPPPPPPAPGLTPARKFSSTSSLAAPGRPHAAALAHGLARSPSWAADRSKDPPGRAPLPMGLGPLAPPP
  QPLPGELEPGDAASKRKR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001003406
• ORF Size: 6564 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001003406.1, NP_001003406.1
• RefSeq Size: 9902
• RefSeq ORF: 6567
• Locus ID: 8911
• Protein Families: Druggable Genome, Ion Channels: Calcium, Transmembrane
• Protein Pathways: Calcium signaling pathway, MAPK signaling pathway
• MW: 241.3 kDa
• Gene Summary: This gene encodes the pore-forming alpha subunit of a voltage gated calcium channel. The encoded protein is a member of a subfamily of calcium channels referred to as is a low voltage-activated, T-type, calcium channel. The channel encoded by this protein is characterized by a slower activation and inactivation compared to other T-type calcium channels. This protein may be involved in calcium signaling in neurons. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2011]