HSF4 (NM_001040667) Human Tagged ORF Clone
CAT#: RC219247
HSF4 (Myc-DDK-tagged)-Human heat shock transcription factor 4 (HSF4), transcript variant 2
"NM_001040667" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | HSF4 |
Synonyms | CTM; CTRCT5 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC219247 representing NM_001040667
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGGAAGCGCCAGCTGCGCTGCCCACGGAGCCAGGCCCCAGCCCCGTGCCTGCCTTCCTCGGCAAGC TATGGGCGCTGGTGGGGGACCCAGGCACAGACCACCTGATCCGCTGGAGCCCGAGCGGGACCAGTTTCCT CGTAAGCGACCAGAGCCGTTTCGCCAAGGAAGTGCTGCCCCAGTATTTCAAGCATAGCAACATGGCGAGC TTCGTGCGCCAACTCAACATGTACGGTTTTCGGAAGGTGGTGAGCATCGAGCAGGGCGGCCTGCTTAGGC CGGAGCGCGACCACGTCGAGTTCCAGCACCCGAGCTTCGTGCGCGGCCGCGAGCAGCTACTGGAGCGCGT GCGGCGCAAGGTGCCCGCGCTGCGCGGCGACGACGGCCGCTGGCGCCCGGAGGACCTGGGTCGACTACTG GGCGAGGTGCAGGCTTTGCGGGGAGTGCAGGAGAGCACCGAGGCGCGGCTGCGGGAGCTCAGGCAGCAGA ACGAGATCTTGTGGCGGGAGGTGGTGACACTTCGGCAGAGCCACGGTCAGCAGCACCGGGTCATTGGCAA GCTGATCCAGTGTCTCTTTGGGCCACTTCAGGCGGGGCCGAGCAATGCAGGAGGCAAGAGAAAGCTGTCC CTGATGCTGGATGAGGGGAGCTCATGCCCAACACCTGCCAAGTTCAACACCTGCCCTCTACCTGGTGCCC TTCTGCAGGACCCCTACTTCATCCAGTCGCCTCTCCCAGAGACAAATTTGGGCCTTAGCCCTCACAGGGC CAGGGGCCCCATCATCTCTGACATCCCAGAAGACTCTCCATCCCCTGAGGGGACCAGGCTTTCTCCCTCC AGTGATGGCAGGAGGGAGAAGGGCCTGGCACTGCTCAAAGAAGAGCCGGCCAGTCCAGGGGGGGATGGCG AGGCCGGGCTGGCCCTGGCCCCAAACGAGTGTGACTTCTGCGTGACAGCCCCCCCGCCACTGCCTGTGGC TGTGGTGCAGGCCATCCTGGAAGGGAAAGGGAGCTTCAGCCCCGAGGGGCCCAGGAATGCCCAACAGCCT GAACCAGGGGATCCCAGGGAGATACCTGACAGGGGGCCTCTGGGCCTGGAAAGCGGGGACAGGAGCCCAG AGAGTCTGCTGCCTCCGATGCTGCTTCAGCCCCCTCAAGAAAGTGTGGAACCTGCAGGGCCTCTAGATGT GCTGGGCCCCAGTCTCCAAGGGCGAGAATGGACCCTGATGGACTTGGACATGGAGCTGTCCTTGATGCAG CCCTTGGTTCCAGAGCGGGGTGAGCCTGAGCTGGCGGTCAAGGGGTTAAATTCTCCAAGCCCAGGGAAGG ACCCCACGCTCGGGGCCCCACTCCTGCTGGATGTCCAGGCGGCCTTGGGAGGCCCAGCCCTGGGCCTGCC TGGGGCTTTAACCATTTATAGCACTCCTGAGAGCCGGACTGCCTCCTACTTGGGCCCGGAAGCCAGTCCC TCCCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219247 representing NM_001040667
Red=Cloning site Green=Tags(s) MQEAPAALPTEPGPSPVPAFLGKLWALVGDPGTDHLIRWSPSGTSFLVSDQSRFAKEVLPQYFKHSNMAS FVRQLNMYGFRKVVSIEQGGLLRPERDHVEFQHPSFVRGREQLLERVRRKVPALRGDDGRWRPEDLGRLL GEVQALRGVQESTEARLRELRQQNEILWREVVTLRQSHGQQHRVIGKLIQCLFGPLQAGPSNAGGKRKLS LMLDEGSSCPTPAKFNTCPLPGALLQDPYFIQSPLPETNLGLSPHRARGPIISDIPEDSPSPEGTRLSPS SDGRREKGLALLKEEPASPGGDGEAGLALAPNECDFCVTAPPPLPVAVVQAILEGKGSFSPEGPRNAQQP EPGDPREIPDRGPLGLESGDRSPESLLPPMLLQPPQESVEPAGPLDVLGPSLQGREWTLMDLDMELSLMQ PLVPERGEPELAVKGLNSPSPGKDPTLGAPLLLDVQAALGGPALGLPGALTIYSTPESRTASYLGPEASP SP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001040667 |
ORF Size | 1476 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001040667.1, NM_001040667.2, NP_001035757.1 |
RefSeq Size | 2535 bp |
RefSeq ORF | 1479 bp |
Locus ID | 3299 |
Cytogenetics | 16q22.1 |
Protein Families | Druggable Genome, Transcription Factors |
MW | 52.8 kDa |
Gene Summary | 'Heat-shock transcription factors (HSFs) activate heat-shock response genes under conditions of heat or other stresses. HSF4 lacks the carboxyl-terminal hydrophobic repeat which is shared among all vertebrate HSFs and has been suggested to be involved in the negative regulation of DNA binding activity. Two alternatively spliced transcripts encoding distinct isoforms and possessing different transcriptional activity have been described. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC311161 | HSF4 (untagged)-Human heat shock transcription factor 4 (HSF4), transcript variant 2 |
USD 830.00 |
|
RG219247 | HSF4 (GFP-tagged) - Human heat shock transcription factor 4 (HSF4), transcript variant 2 |
USD 500.00 |
|
RC219247L1 | Lenti ORF clone of Human heat shock transcription factor 4 (HSF4), transcript variant 2, Myc-DDK-tagged |
USD 804.00 |
|
RC219247L2 | Lenti ORF clone of Human heat shock transcription factor 4 (HSF4), transcript variant 2, mGFP tagged |
USD 650.00 |
|
RC219247L3 | Lenti ORF clone of Human heat shock transcription factor 4 (HSF4), transcript variant 2, Myc-DDK-tagged |
USD 650.00 |
|
RC219247L4 | Lenti ORF clone of Human heat shock transcription factor 4 (HSF4), transcript variant 2, mGFP tagged |
USD 650.00 |
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