HSF4 (NM_001040667) Human Untagged Clone

CAT#: SC311161

HSF4 (untagged)-Human heat shock transcription factor 4 (HSF4), transcript variant 2


  "NM_001040667" in other vectors (6)

Reconstitution Protocol

USD 830.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol HSF4
Synonyms CTM; CTRCT5
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_001040667 edited
GCCCGCAGCGGCCGGGCCCGAGCGCAGAGCCGGGCCGAGACTGCACCATGCAGGAAGCGC
CAGCTGCGCTGCCCACGGAGCCAGGCCCCAGCCCCGTGCCTGCCTTCCTCGGCAAGCTAT
GGGCGCTGGTGGGGGACCCAGGCACAGACCACCTGATCCGCTGGAGCCCGAGCGGGACCA
GTTTCCTCGTAAGCGACCAGAGCCGTTTCGCCAAGGAAGTGCTGCCCCAGTATTTCAAGC
ATAGCAACATGGCGAGCTTCGTGCGCCAACTCAACATGTACGGTTTTCGGAAGGTGGTGA
GCATCGAGCAGGGCGGCCTGCTTAGGCCGGAGCGCGACCACGTCGAGTTCCAGCACCCGA
GCTTCGTGCGCGGCCGCGAGCAGCTACTGGAGCGCGTGCGGCGCAAGGTGCCCGCGCTGC
GCGGCGACGACGGCCGCTGGCGCCCGGAGGACCTGGGTCGACTACTGGGCGAGGTGCAGG
CTTTGCGGGGAGTGCAGGAGAGCACCGAGGCGCGGCTGCGGGAGCTCAGGCAGCAGAACG
AGATCTTGTGGCGGGAGGTGGTGACACTTCGGCAGAGCCACGGTCAGCAGCACCGGGTCA
TTGGCAAGCTGATCCAGTGTCTCTTTGGGCCACTTCAGGCGGGGCCGAGCAATGCAGGAG
GCAAGAGAAAGCTGTCCCTGATGCTGGATGAGGGGAGCTCATGCCCAACACCTGCCAAGT
TCAACACCTGCCCTCTACCTGGTGCCCTTCTGCAGGACCCCTACTTCATCCAGTCGCCTC
TCCCAGAGACAAATTTGGGCCTTAGCCCTCACAGGGCCAGGGGCCCCATCATCTCTGACA
TCCCAGAAGACTCTCCATCCCCTGAGGGGACCAGGCTTTCTCCCTCCAGTGATGGCAGGA
GGGAGAAGGGCCTGGCACTGCTCAAAGAAGAGCCGGCCAGTCCAGGGGGGGATGGCGAGG
CCGGGCTGGCCCTGGCCCCAAACGAGTGTGACTTCTGCGTGACAGCCCCCCCGCCACTGC
CTGTGGCTGTGGTGCAGGCCATCCTGGAAGGGAAAGGGAGCTTCAGCCCCGAGGGGCCCA
GGAATGCCCAACAGCCTGAACCAGGGGATCCCAGGGAGATACCTGACAGGGGGCCTCTGG
GCCTGGAAAGCGGGGACAGGAGCCCAGAGAGTCTGCTGCCTCCGATGCTGCTTCAGCCCC
CTCAAGAAAGTGTGGAACCTGCAGGGCCTCTAGATGTGCTGGGCCCCAGTCTCCAAGGGC
GAGAATGGACCCTGATGGACTTGGACATGGAGCTGTCCTTGATGCAGCCCTTGGTTCCAG
AGCGGGGTGAGCCTGAGCTGGCGGTCAAGGTGTTAAATTCTCCAAGCCCAGGGAAGGACC
CCACGCTCGGGGCCCCACTCCTGCTGGATGTCCAGGCGGCCTTGGGAGGCCCAGCCCTGG
GCCTGCCTGGGGCTTTAACCATTTATAGCACTCCTGAGAGCCGGACTGCCTCCTACTTGG
GCCCGGAAGCCAGTCCCTCCCCCTAAGACCCCGCGCCTCTGAAGGGGCTTGGAACCAGTC
CGCCGCTGCACATCCTTCTTGGCTTCCTGGCGCCCCCTATCGGGGGTGAGCGAAGCCCCC
ACTACTAAATGGCCTCTCTCCACTACCCCGACTATCCCTGCACATAAACTCCG
>OriGene 5' read for NM_001040667 unedited
NGGGTTAGATATTTGTATACGACTCACTATAGGCGGCCGCGCAATTCGGCACGAGGGCCC
GCAGCGGCCGGGCCCGAGCGCAGAGCCGGGCCGAGACTGCACCATGCAGGAAGCGCCAGC
TGCGCTGCCCACGGAGCCAGGCCCCAGCCCCGTGCCTGCCTTCCTCGGCAAGCTATGGGC
GCTGGTGGGGGACCCAGGCACAGACCACCTGATCCGCTGGAGCCCGAGCGGGACCAGTTT
CCTCGTAAGCGACCAGAGCCGTTTCGCCAAGGAAGTGCTGCCCCAGTATTTCAAGCATAG
CAACATGGCGAGCTTCGTGCGCCAACTCAACATGTACGGTTTTCGGAAGGTGGTGAGCAT
CGAGCAGGGCGGCCTGCTTAGGCCGGAGCGCGACCACGTCGAGTTCCAGCACCCGAGCTT
CGTGCGCGGCCGCGAGCAGCTACTGGAGCGCGTGCGGCGCAAGGTGCCCGCGCTGCGCGG
CGACGACGGCCGCTGGCGCCCGGAGGACCTGGGTCGACTACTGGGCGAGGTGCAGGCTTT
GCGGGGAGTGCAGGAGAGCACCGAGGCGCGGCTGCGGGAGCTCAGGCAGCAGAACGAGAT
CTTGTGGCGGGAGGTGGTGACACTTCGGCAGAGCCACGGTCAGCAGCACCGGGTCATTGG
CAAGCTGATCCAGTGTCTCTTTGGGCCACTTCAGGCGGNGCCGAGCAATGCAGGAGGCAA
GAGAAAGCTGTCCCTGATGCTGGATGAGGGGAGCTCATGCCCAACACCTGCCAAGTTCAA
CACCTGCCCTCTACCTGGTGCCCCTCTGCNAGACCCCTACTTCATCCAGTCGCCTCTCCC
AGAGACAAATTTGGGGCCTAGCCCTCACAGGGCCCAGGGCCCCATCATCTCT
>Forward primer walk for NM_001040667 unedited
ATCTTTGGGAGTCANAAGAGGGGCCCCTGGCCCTGTGAGGCTAAGGCCCAAATTGTCTCT
GGGAGAGGCGACTGGATGAAGTAGGGGTCCTGCAGAAGGGCACCAGGTAGAGGGCAGGTG
TTGAACTTGGCAGGTGTTGGGCATGAGCTCCCCTCATCCAGCATCAGGGACAGCTTTCTC
TTGCCTCCTGCATTGCTCGGCCCCGCCTGAAGTGGCCCAAAGAGACACTGGATCAGCTTG
CCAATGACCCGGTGCTGCTGACCGTGGCTCTGCCGAAGTGTCACCACCTCCCGCCACAAG
ATCTCGTTCTGCTGCCTGAGCTCCCGCAGCCGCGCCTCGGTGCTCTCCTGCACTCCCCGC
AAAGCCTGCACCTCGCCCAGTAGTCGACCCAGGTCCTCCGGGCGCCAGCGGCCGTCGTCG
CCGCGCAGCGCGGGCACCTTGCGCCGCACGCGCTCCAGTAGCTGCTCGCGGCCGCGCACG
AAGCTCGGGTGCTGGAACTCGACGTGGTCGCGCTCCGGCCTAAGCAGGCCGCCCTGCTCG
ATGCTCACCACCTTCCGAAAACCGTACATGTTGAGTTGGCGCACGAAGCTCGCCATGTTG
CTATGCTTGAAATACTGGGGCAGCACTTCCTTGGCGAAACGGCTCTGGTCGCTTACGAGG
AAACTGGTCCCGCTNCGGCTCCAGCGGATCAGGTGGTCTGTGCCTGGGTCCCCCACCAGC
GCCCATAGCTTTGCGAGGAAGGCANGCACNGNGCTGGGGCCTGGCTCCGTGGGCAGCGCA
GCTGGCGCTTCCTGCATGGTGCAGTCTCGGGCCCGCTCTGCGCTCGGGCCCCGCCGCTGC
GGGCCCTCG
Restriction Sites Please inquire     
ACCN NM_001040667
Insert Size 1700 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation There is 1 nucleotide difference between the OriGene clone and the NCBI reference ORF. OriGene considers these to be polymorphisms and to reflect the natural differences between individuals. These result in the substitution of 1 amino acids.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001040667.1, NP_001035757.1
RefSeq Size 2535 bp
RefSeq ORF 1479 bp
Locus ID 3299
Cytogenetics 16q22.1
Protein Families Druggable Genome, Transcription Factors
Gene Summary 'Heat-shock transcription factors (HSFs) activate heat-shock response genes under conditions of heat or other stresses. HSF4 lacks the carboxyl-terminal hydrophobic repeat which is shared among all vertebrate HSFs and has been suggested to be involved in the negative regulation of DNA binding activity. Two alternatively spliced transcripts encoding distinct isoforms and possessing different transcriptional activity have been described. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (2) uses two alternate in-frame splice sites in the 3' coding region, compared to variant 1, resulting in a longer protein (isoform b). This variant, alternatively referred to as HSF4b, functions as a transcriptional activator of the constitutive expression of heat shock genes. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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