HSF4 (NM_001040667) Human Untagged Clone

CAT#: SC311161

HSF4 (untagged)-Human heat shock transcription factor 4 (HSF4), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HSF4
• Synonyms: CTM; CTRCT5
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001040667 edited
  GCCCGCAGCGGCCGGGCCCGAGCGCAGAGCCGGGCCGAGACTGCACCATGCAGGAAGCGC
  CAGCTGCGCTGCCCACGGAGCCAGGCCCCAGCCCCGTGCCTGCCTTCCTCGGCAAGCTAT
  GGGCGCTGGTGGGGGACCCAGGCACAGACCACCTGATCCGCTGGAGCCCGAGCGGGACCA
  GTTTCCTCGTAAGCGACCAGAGCCGTTTCGCCAAGGAAGTGCTGCCCCAGTATTTCAAGC
  ATAGCAACATGGCGAGCTTCGTGCGCCAACTCAACATGTACGGTTTTCGGAAGGTGGTGA
  GCATCGAGCAGGGCGGCCTGCTTAGGCCGGAGCGCGACCACGTCGAGTTCCAGCACCCGA
  GCTTCGTGCGCGGCCGCGAGCAGCTACTGGAGCGCGTGCGGCGCAAGGTGCCCGCGCTGC
  GCGGCGACGACGGCCGCTGGCGCCCGGAGGACCTGGGTCGACTACTGGGCGAGGTGCAGG
  CTTTGCGGGGAGTGCAGGAGAGCACCGAGGCGCGGCTGCGGGAGCTCAGGCAGCAGAACG
  AGATCTTGTGGCGGGAGGTGGTGACACTTCGGCAGAGCCACGGTCAGCAGCACCGGGTCA
  TTGGCAAGCTGATCCAGTGTCTCTTTGGGCCACTTCAGGCGGGGCCGAGCAATGCAGGAG
  GCAAGAGAAAGCTGTCCCTGATGCTGGATGAGGGGAGCTCATGCCCAACACCTGCCAAGT
  TCAACACCTGCCCTCTACCTGGTGCCCTTCTGCAGGACCCCTACTTCATCCAGTCGCCTC
  TCCCAGAGACAAATTTGGGCCTTAGCCCTCACAGGGCCAGGGGCCCCATCATCTCTGACA
  TCCCAGAAGACTCTCCATCCCCTGAGGGGACCAGGCTTTCTCCCTCCAGTGATGGCAGGA
  GGGAGAAGGGCCTGGCACTGCTCAAAGAAGAGCCGGCCAGTCCAGGGGGGGATGGCGAGG
  CCGGGCTGGCCCTGGCCCCAAACGAGTGTGACTTCTGCGTGACAGCCCCCCCGCCACTGC
  CTGTGGCTGTGGTGCAGGCCATCCTGGAAGGGAAAGGGAGCTTCAGCCCCGAGGGGCCCA
  GGAATGCCCAACAGCCTGAACCAGGGGATCCCAGGGAGATACCTGACAGGGGGCCTCTGG
  GCCTGGAAAGCGGGGACAGGAGCCCAGAGAGTCTGCTGCCTCCGATGCTGCTTCAGCCCC
  CTCAAGAAAGTGTGGAACCTGCAGGGCCTCTAGATGTGCTGGGCCCCAGTCTCCAAGGGC
  GAGAATGGACCCTGATGGACTTGGACATGGAGCTGTCCTTGATGCAGCCCTTGGTTCCAG
  AGCGGGGTGAGCCTGAGCTGGCGGTCAAGGTGTTAAATTCTCCAAGCCCAGGGAAGGACC
  CCACGCTCGGGGCCCCACTCCTGCTGGATGTCCAGGCGGCCTTGGGAGGCCCAGCCCTGG
  GCCTGCCTGGGGCTTTAACCATTTATAGCACTCCTGAGAGCCGGACTGCCTCCTACTTGG
  GCCCGGAAGCCAGTCCCTCCCCCTAAGACCCCGCGCCTCTGAAGGGGCTTGGAACCAGTC
  CGCCGCTGCACATCCTTCTTGGCTTCCTGGCGCCCCCTATCGGGGGTGAGCGAAGCCCCC
  ACTACTAAATGGCCTCTCTCCACTACCCCGACTATCCCTGCACATAAACTCCG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001040667 unedited
  NGGGTTAGATATTTGTATACGACTCACTATAGGCGGCCGCGCAATTCGGCACGAGGGCCC
  GCAGCGGCCGGGCCCGAGCGCAGAGCCGGGCCGAGACTGCACCATGCAGGAAGCGCCAGC
  TGCGCTGCCCACGGAGCCAGGCCCCAGCCCCGTGCCTGCCTTCCTCGGCAAGCTATGGGC
  GCTGGTGGGGGACCCAGGCACAGACCACCTGATCCGCTGGAGCCCGAGCGGGACCAGTTT
  CCTCGTAAGCGACCAGAGCCGTTTCGCCAAGGAAGTGCTGCCCCAGTATTTCAAGCATAG
  CAACATGGCGAGCTTCGTGCGCCAACTCAACATGTACGGTTTTCGGAAGGTGGTGAGCAT
  CGAGCAGGGCGGCCTGCTTAGGCCGGAGCGCGACCACGTCGAGTTCCAGCACCCGAGCTT
  CGTGCGCGGCCGCGAGCAGCTACTGGAGCGCGTGCGGCGCAAGGTGCCCGCGCTGCGCGG
  CGACGACGGCCGCTGGCGCCCGGAGGACCTGGGTCGACTACTGGGCGAGGTGCAGGCTTT
  GCGGGGAGTGCAGGAGAGCACCGAGGCGCGGCTGCGGGAGCTCAGGCAGCAGAACGAGAT
  CTTGTGGCGGGAGGTGGTGACACTTCGGCAGAGCCACGGTCAGCAGCACCGGGTCATTGG
  CAAGCTGATCCAGTGTCTCTTTGGGCCACTTCAGGCGGNGCCGAGCAATGCAGGAGGCAA
  GAGAAAGCTGTCCCTGATGCTGGATGAGGGGAGCTCATGCCCAACACCTGCCAAGTTCAA
  CACCTGCCCTCTACCTGGTGCCCCTCTGCNAGACCCCTACTTCATCCAGTCGCCTCTCCC
  AGAGACAAATTTGGGGCCTAGCCCTCACAGGGCCCAGGGCCCCATCATCTCT
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_001040667 unedited
  ATCTTTGGGAGTCANAAGAGGGGCCCCTGGCCCTGTGAGGCTAAGGCCCAAATTGTCTCT
  GGGAGAGGCGACTGGATGAAGTAGGGGTCCTGCAGAAGGGCACCAGGTAGAGGGCAGGTG
  TTGAACTTGGCAGGTGTTGGGCATGAGCTCCCCTCATCCAGCATCAGGGACAGCTTTCTC
  TTGCCTCCTGCATTGCTCGGCCCCGCCTGAAGTGGCCCAAAGAGACACTGGATCAGCTTG
  CCAATGACCCGGTGCTGCTGACCGTGGCTCTGCCGAAGTGTCACCACCTCCCGCCACAAG
  ATCTCGTTCTGCTGCCTGAGCTCCCGCAGCCGCGCCTCGGTGCTCTCCTGCACTCCCCGC
  AAAGCCTGCACCTCGCCCAGTAGTCGACCCAGGTCCTCCGGGCGCCAGCGGCCGTCGTCG
  CCGCGCAGCGCGGGCACCTTGCGCCGCACGCGCTCCAGTAGCTGCTCGCGGCCGCGCACG
  AAGCTCGGGTGCTGGAACTCGACGTGGTCGCGCTCCGGCCTAAGCAGGCCGCCCTGCTCG
  ATGCTCACCACCTTCCGAAAACCGTACATGTTGAGTTGGCGCACGAAGCTCGCCATGTTG
  CTATGCTTGAAATACTGGGGCAGCACTTCCTTGGCGAAACGGCTCTGGTCGCTTACGAGG
  AAACTGGTCCCGCTNCGGCTCCAGCGGATCAGGTGGTCTGTGCCTGGGTCCCCCACCAGC
  GCCCATAGCTTTGCGAGGAAGGCANGCACNGNGCTGGGGCCTGGCTCCGTGGGCAGCGCA
  GCTGGCGCTTCCTGCATGGTGCAGTCTCGGGCCCGCTCTGCGCTCGGGCCCCGCCGCTGC
  GGGCCCTCG
  
• Restriction Sites: Please inquire
• ACCN: NM_001040667
• Insert Size: 1700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: There is 1 nucleotide difference between the OriGene clone and the NCBI reference ORF. OriGene considers these to be polymorphisms and to reflect the natural differences between individuals. These result in the substitution of 1 amino acids.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001040667.1, NP_001035757.1
• RefSeq Size: 2535 bp
• RefSeq ORF: 1479 bp
• Locus ID: 3299
• Cytogenetics: 16q22.1
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'Heat-shock transcription factors (HSFs) activate heat-shock response genes under conditions of heat or other stresses. HSF4 lacks the carboxyl-terminal hydrophobic repeat which is shared among all vertebrate HSFs and has been suggested to be involved in the negative regulation of DNA binding activity. Two alternatively spliced transcripts encoding distinct isoforms and possessing different transcriptional activity have been described. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) uses two alternate in-frame splice sites in the 3' coding region, compared to variant 1, resulting in a longer protein (isoform b). This variant, alternatively referred to as HSF4b, functions as a transcriptional activator of the constitutive expression of heat shock genes. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.