SIGLECL1 (SIGLEC12) (NM_033329) Human Tagged ORF Clone

CAT#: RC219265

SIGLEC12 (Myc-DDK-tagged)-Human sialic acid binding Ig-like lectin 12 (SIGLEC12), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY409587) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219265 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SIGLEC12
• Synonyms: S2V; Siglec-XII; SIGLECL1; SLG
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219265 representing NM_033329
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCTGCCCCTGCTATGGGCAAATGAAGAGAGGGACAGTGGGGGCTGGGCTGACCCTCGTTTCTCCA
  CAGCGTCCCAGGACCTACTGTCAAGATACAGGCTGGAGGTGCCAGAGTCGGTGACTGTGCAGGAGGGTCT
  GTGTGTCTCTGTGCCCTGCAGTGTCCTTTACCCCCATTACAACTGGACTGCCTCTAGCCCTGTTTATGGA
  TCCTGGTTCAAGGAAGGGGCCGATATACCATGGGATATTCCAGTGGCCACAAACACCCCAAGTGGAAAAG
  TGCAAGAGGATACCCACGGTCGATTCCTCCTCCTTGGGGACCCACAGACCAACAACTGCTCCCTGAGCAT
  CAGAGATGCCAGGAAGGGGGATTCAGGGAAGTACTACTTCCAGGTGGAGAGAGGAAGCAGGAAATGGAAC
  TACATATATGACAAGCTCTCTGTGCATGTGACAGCCCTGACTCACATGCCCACCTTCTCCATCCCGGGGA
  CCCTGGAGTCTGGCCACCCCAGGAACCTGACCTGCTCTGTGCCCTGGGCCTGTGAACAGGGGACGCCCCC
  CACGATCACCTGGATGGGGGCCTCCGTGTCCTCCCTGGACCCCACTATCACTCGCTCCTCGATGCTCAGC
  CTCATCCCACAGCCCCAGGACCATGGCACCAGCCTCACCTGTCAGGTGACCTTGCCTGGGGCCGGCGTGA
  CCATGACCAGGGCTGTCCGACTCAACATATCCTATCCTCCTCAGAACTTGACCATGACTGTCTTCCAAGG
  AGATGGCACAGCATCCACAACCTTGAGGAATGGCTCGGCCCTTTCAGTCCTGGAGGGCCAGTCCCTGCAC
  CTTGTCTGTGCTGTCGACAGCAATCCCCCTGCCAGGCTGAGCTGGACCTGGGGGAGCCTGACCCTGAGCC
  CCTCACAGTCCTCGAACCTTGGGGTGCTGGAGCTGCCTCGAGTGCATGTGAAGGATGAAGGGGAATTCAC
  CTGCCGAGCTCAGAACCCTCTAGGCTCCCAGCACATTTCCCTGAGCCTCTCCCTGCAAAACGAGTACACA
  GGCAAAATGAGGCCTATATCAGGAGTGACGCTAGGGGCATTCGGGGGAGCTGGAGCCACAGCCCTGGTCT
  TCCTGTACTTCTGCATCATCTTCGTTGTAGTGAGGTCCTGCAGGAAGAAATCGGCAAGGCCAGCAGTGGG
  CGTGGGGGATACAGGCATGGAGGACGCAAACGCTGTCAGGGGCTCAGCCTCTCAGGGACCCCTGATTGAA
  TCCCCGGCAGATGACAGCCCCCCACACCATGCTCCGCCAGCCCTGGCCACCCCCTCCCCAGAGGAAGGAG
  AGATCCAGTATGCATCCCTCAGCTTCCACAAAGCGAGGCCTCAGTACCCACAGGAACAGGAGGCCATCGG
  CTATGAGTACTCCGAGATCAACATCCCCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219265 representing NM_033329
  Red=Cloning site Green=Tags(s)
  
  MLLPLLWANEERDSGGWADPRFSTASQDLLSRYRLEVPESVTVQEGLCVSVPCSVLYPHYNWTASSPVYG
  SWFKEGADIPWDIPVATNTPSGKVQEDTHGRFLLLGDPQTNNCSLSIRDARKGDSGKYYFQVERGSRKWN
  YIYDKLSVHVTALTHMPTFSIPGTLESGHPRNLTCSVPWACEQGTPPTITWMGASVSSLDPTITRSSMLS
  LIPQPQDHGTSLTCQVTLPGAGVTMTRAVRLNISYPPQNLTMTVFQGDGTASTTLRNGSALSVLEGQSLH
  LVCAVDSNPPARLSWTWGSLTLSPSQSSNLGVLELPRVHVKDEGEFTCRAQNPLGSQHISLSLSLQNEYT
  GKMRPISGVTLGAFGGAGATALVFLYFCIIFVVVRSCRKKSARPAVGVGDTGMEDANAVRGSASQGPLIE
  SPADDSPPHHAPPALATPSPEEGEIQYASLSFHKARPQYPQEQEAIGYEYSEINIPK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033329
• ORF Size: 1431 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_033329.1, NM_033329.2, NP_201586.1
• RefSeq Size: 1736
• RefSeq ORF: 1434
• Locus ID: 89858
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transmembrane
• MW: 50.1 kDa
• Gene Summary: Sialic acid-binding immunoglobulin-like lectins (SIGLECs) are a family of cell surface proteins belonging to the immunoglobulin superfamily. They mediate protein-carbohydrate interactions by selectively binding to different sialic acid moieties present on glycolipids and glycoproteins. This gene encodes a member of the SIGLEC3-like subfamily of SIGLECs. Members of this subfamily are characterized by an extracellular V-set immunoglobulin-like domain followed by two C2-set immunoglobulin-like domains, and the cytoplasmic tyrosine-based motifs ITIM and SLAM-like. The encoded protein, upon tyrosine phosphorylation, has been shown to recruit the Src homology 2 domain-containing protein-tyrosine phosphatases SHP1 and SHP2. It has been suggested that the protein is involved in the negative regulation of macrophage signaling by functioning as an inhibitory receptor. This gene is located in a cluster with other SIGLEC3-like genes on 19q13.4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]