ADH6 (NM_000672) Human Tagged ORF Clone

CAT#: RC219471

ADH6 (Myc-DDK-tagged)-Human alcohol dehydrogenase 6 (class V) (ADH6), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ADH6
• Synonyms: ADH-5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219471 representing NM_000672
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTACTACAGGCCAAGTCATCAGATGCAAAGCAGCCATACTCTGGAAGCCTGGTGCACCATTTTCTA
  TTGAAGAGGTAGAAGTGGCCCCACCAAAGGCAAAGGAAGTTCGCATAAAGGTTGTGGCCACCGGACTGTG
  TGGTACAGAGATGAAAGTGTTGGGGAGTAAACACTTGGACCTCTTGTATCCCACCATCTTGGGCCATGAA
  GGGGCTGGAATCGTTGAGAGTATTGGAGAAGGAGTAAGCACAGTGAAACCAGGTGACAAAGTTATCACAC
  TCTTTCTGCCACAGTGTGGAGAATGTACCTCTTGCCTGAATTCTGAGGGCAATTTTTGTATACAATTCAA
  ACAGTCAAAAACCCAACTGATGTCTGATGGTACCAGCAGGTTTACCTGCAAGGGAAAATCAATATATCAC
  TTTGGTAATACCAGCACCTTCTGTGAATACACAGTGATAAAGGAAATCTCAGTTGCCAAGATTGATGCAG
  TCGCTCCTCTAGAGAAAGTATGCCTAATTAGCTGTGGCTTTTCCACTGGGTTTGGTGCTGCAATCAATAC
  TGCCAAGGTGACTCCAGGTTCTACCTGTGCTGTGTTTGGCCTGGGAGGAGTCGGCTTGTCTGTTGTCATG
  GGTTGTAAAGCAGCAGGAGCAGCCAGGATCATTGGAGTGGATGTCAACAAGGAGAAATTTAAGAAGGCAC
  AGGAATTGGGTGCTACTGAGTGCCTCAACCCTCAGGACTTAAAGAAACCCATTCAAGAAGTTTTATTTGA
  TATGACAGATGCTGGTATAGACTTCTGCTTTGAGGCCATTGGAAATCTGGACGTTCTGGCAGCTGCCCTC
  GCCTCCTGCAATGAGAGCTATGGGGTCTGTGTGGTTGTTGGGGTGTTGCCTGCCAGTGTTCAACTCAAAA
  TCAGTGGCCAGTTGTTCTTCTCAGGACGTTCTTTGAAGGGTTCTGTTTTTGGAGGCTGGAAGAGCAGACA
  GCACATCCCTAAACTGGTTGCTGATTATATGGCAGAGAAGTTGAATCTAGATCCACTAATTACTCATACT
  CTGAATCTTGATAAAATCAATGAAGCAGTTGAATTAATGAAAACTGGAAAATGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219471 representing NM_000672
  Red=Cloning site Green=Tags(s)
  
  MSTTGQVIRCKAAILWKPGAPFSIEEVEVAPPKAKEVRIKVVATGLCGTEMKVLGSKHLDLLYPTILGHE
  GAGIVESIGEGVSTVKPGDKVITLFLPQCGECTSCLNSEGNFCIQFKQSKTQLMSDGTSRFTCKGKSIYH
  FGNTSTFCEYTVIKEISVAKIDAVAPLEKVCLISCGFSTGFGAAINTAKVTPGSTCAVFGLGGVGLSVVM
  GCKAAGAARIIGVDVNKEKFKKAQELGATECLNPQDLKKPIQEVLFDMTDAGIDFCFEAIGNLDVLAAAL
  ASCNESYGVCVVVGVLPASVQLKISGQLFFSGRSLKGSVFGGWKSRQHIPKLVADYMAEKLNLDPLITHT
  LNLDKINEAVELMKTGKW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000672
• ORF Size: 1104 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000672.1, NM_000672.2, NM_000672.3, NP_000663.1
• RefSeq Size: 1398 bp
• RefSeq ORF: 1107 bp
• Locus ID: 130
• Cytogenetics: 4q23
• Domains: ADH_zinc_N
• Protein Families: Druggable Genome
• Protein Pathways: Drug metabolism - cytochrome P450, Fatty acid metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Retinol metabolism, Tyrosine metabolism
• MW: 38.9 kDa
• Gene Summary: 'This gene encodes class V alcohol dehydrogenase, which is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This gene is expressed in the stomach as well as in the liver, and it contains a glucocorticoid response element upstream of its 5' UTR, which is a steroid hormone receptor binding site. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'