HSPA4 (NM_002154) Human Tagged ORF Clone

CAT#: RC220635

HSPA4 (Myc-DDK-tagged)-Human heat shock 70kDa protein 4 (HSPA4)

Product Images

Western blot validation of overexpression lysate (Cat# LY400782) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220635 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified HSPA4 protein (Cat# TP320635). The protein was produced from HEK293T cells transfected with HSPA4 cDNA clone (Cat# RC220635) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HSPA4
• Synonyms: APG-2; HEL-S-5a; HS24/P52; hsp70; hsp70RY; HSPH2; RY
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220635 representing NM_002154
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGTGGTGGGCATAGACCTGGGCTTCCAGAGCTGCTACGTCGCTGTGGCCCGCGCCGGCGGCATCG
  AGACTATCGCTAATGAGTATAGCGACCGCTGCACGCCGGCTTGCATTTCTTTTGGTCCTAAGAATCGTTC
  AATTGGAGCAGCAGCTAAAAGCCAGGTAATTTCTAATGCAAAGAACACAGTCCAAGGATTTAAAAGATTC
  CATGGCCGAGCATTCTCTGATCCATTTGTGGAGGCAGAAAAATCTAACCTTGCATATGATATTGTGCAGT
  TGCCTACAGGATTAACAGGTATAAAGGTGACATATATGGAGGAAGAGCGAAATTTTACCACTGAGCAAGT
  GACTGCCATGCTTTTGTCCAAACTGAAGGAGACAGCCGAAAGTGTTCTTAAGAAGCCTGTAGTTGACTGT
  GTTGTTTCGGTTCCTTGTTTCTATACTGATGCAGAAAGACGATCAGTGATGGATACAACACAGATTGCTG
  GTCTTAATTGCTTGCGATTAATGAATGAAACCACTGCAGTTGCTCTTGCATATGGAATCTATAAGCAGGA
  TCTTCCTGCCTTAGAAGAGAAACCAAGAAATGTAGTTTTTGTAGACATGGGCCACTCTGCTTATCAAGTT
  TCTGTATGTGCATTTAATAGAGGAAAACTGAAAGTTCTGGCCACTGCATTTGACACGACATTGGGAGGTA
  GAAAATTTGATGAAGTGTTAGTAAATCACTTCTGTGAAGAATTTGGGAAGAAATACAAGCTAGACATTAA
  GTCCAAAATCCGTGCATTATTACGACTCTCTCAGGAGTGTGAGAAACTCAAGAAATTGATGAGTGCAAAT
  GCTTCAGATCTCCCTTTGAGCATTGAATGTTTTATGAATGATGTTGATGTATCTGGAACTATGAATAGAG
  GCAAATTTCTGGAGATGTGCAATGATCTCTTAGCTAGAGTGGAGCCACCACTTCGTAGTGTTTTGGAACA
  AACCAAGTTAAAGAAAGAAGATATTTATGCAGTGGAGATAGTTGGTGGTGCTACACGAATCCCTGCGGTA
  AAAGAGAAGATCAGCAAATTTTTCGGTAAAGAACTTAGTACAACATTAAATGCTGATGAAGCTGTCACTC
  GAGGCTGTGCATTGCAGTGTGCCATCTTATCGCCTGCTTTCAAAGTCAGAGAATTTTCTATCACTGATGT
  AGTACCATATCCAATATCTCTGAGATGGAATTCTCCAGCTGAAGAAGGGTCAAGTGACTGTGAAGTCTTT
  TCCAAAAATCATGCTGCTCCTTTCTCTAAAGTTCTTACATTTTATAGAAAGGAACCTTTCACTCTTGAGG
  CCTACTACAGCTCTCCTCAGGATTTGCCCTATCCAGATCCTGCTATAGCTCAGTTTTCAGTTCAGAAAGT
  CACTCCTCAGTCTGATGGCTCCAGTTCAAAAGTGAAAGTCAAAGTTCGAGTAAATGTCCATGGCATTTTC
  AGTGTGTCCAGTGCATCTTTAGTGGAGGTTCACAAGTCTGAGGAAAATGAGGAGCCAATGGAAACAGATC
  AGAATGCAAAGGAGGAAGAGAAGATGCAAGTGGACCAGGAGGAACCACATGTTGAAGAGCAACAGCAGCA
  GACACCAGCAGAAAATAAGGCAGAGTCTGAAGAAATGGAGACCTCTCAAGCTGGATCCAAGGATAAAAAG
  ATGGACCAACCACCCCAAGCCAAGAAGGCAAAAGTGAAGACCAGTACTGTGGACCTGCCAATCGAGAATC
  AGCTATTATGGCAGATAGACAGAGAGATGCTCAACTTGTACATTGAAAATGAGGGTAAGATGATCATGCA
  GGATAAACTGGAGAAGGAGCGGAATGATGCTAAGAACGCAGTGGAGGAATATGTGTATGAAATGAGAGAC
  AAGCTTAGTGGTGAATATGAGAAGTTTGTGAGTGAAGATGATCGTAACAGTTTTACTTTGAAACTGGAAG
  ATACTGAAAATTGGTTGTATGAGGATGGAGAAGACCAGCCAAAGCAAGTTTATGTTGATAAGTTGGCTGA
  ATTAAAAAATCTAGGTCAACCTATTAAGATACGTTTCCAGGAATCTGAAGAACGACCAAAATTATTTGAA
  GAACTAGGGAAACAGATCCAACAGTATATGAAAATAATCAGCTCTTTCAAAAACAAGGAGGACCAGTATG
  ATCATTTGGATGCTGCTGACATGACAAAGGTAGAAAAAAGCACAAATGAAGCAATGGAGTGGATGAATAA
  CAAGCTAAATCTGCAGAACAAGCAGAGTTTGACCATGGATCCAGTTGTCAAGTCAAAAGAGATTGAAGCT
  AAAATTAAGGAGCTGACAAGTACTTGTAGCCCTATAATTTCAAAGCCCAAACCCAAAGTGGAACCTCCAA
  AAGAGGAACAAAAAAATGCAGAGCAGAATGGACCAGTGGATGGACAAGGAGACAACCCAGGCCCCCAGGC
  TGCTGAGCAGGGTACAGACACAGCTGTGCCTTCGGATTCAGACAAGAAGCTTCCTGAAATGGACATTGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220635 representing NM_002154
  Red=Cloning site Green=Tags(s)
  
  MSVVGIDLGFQSCYVAVARAGGIETIANEYSDRCTPACISFGPKNRSIGAAAKSQVISNAKNTVQGFKRF
  HGRAFSDPFVEAEKSNLAYDIVQLPTGLTGIKVTYMEEERNFTTEQVTAMLLSKLKETAESVLKKPVVDC
  VVSVPCFYTDAERRSVMDTTQIAGLNCLRLMNETTAVALAYGIYKQDLPALEEKPRNVVFVDMGHSAYQV
  SVCAFNRGKLKVLATAFDTTLGGRKFDEVLVNHFCEEFGKKYKLDIKSKIRALLRLSQECEKLKKLMSAN
  ASDLPLSIECFMNDVDVSGTMNRGKFLEMCNDLLARVEPPLRSVLEQTKLKKEDIYAVEIVGGATRIPAV
  KEKISKFFGKELSTTLNADEAVTRGCALQCAILSPAFKVREFSITDVVPYPISLRWNSPAEEGSSDCEVF
  SKNHAAPFSKVLTFYRKEPFTLEAYYSSPQDLPYPDPAIAQFSVQKVTPQSDGSSSKVKVKVRVNVHGIF
  SVSSASLVEVHKSEENEEPMETDQNAKEEEKMQVDQEEPHVEEQQQQTPAENKAESEEMETSQAGSKDKK
  MDQPPQAKKAKVKTSTVDLPIENQLLWQIDREMLNLYIENEGKMIMQDKLEKERNDAKNAVEEYVYEMRD
  KLSGEYEKFVSEDDRNSFTLKLEDTENWLYEDGEDQPKQVYVDKLAELKNLGQPIKIRFQESEERPKLFE
  ELGKQIQQYMKIISSFKNKEDQYDHLDAADMTKVEKSTNEAMEWMNNKLNLQNKQSLTMDPVVKSKEIEA
  KIKELTSTCSPIISKPKPKVEPPKEEQKNAEQNGPVDGQGDNPGPQAAEQGTDTAVPSDSDKKLPEMDID
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002154
• ORF Size: 2520 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002154.2, NM_002154.3, NP_002145.3
• RefSeq Size: 3385 bp
• RefSeq ORF: 2523 bp
• Locus ID: 3308
• Cytogenetics: 5q31.1
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS
• Protein Pathways: Antigen processing and presentation
• MW: 94.2 kDa
• Gene Summary: ''