HSPA4 (NM_002154) Human Untagged Clone

CAT#: SC107571

HSPA4 (untagged)-Human heat shock 70kDa protein 4 (HSPA4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HSPA4
• Synonyms: APG-2; HEL-S-5a; HS24/P52; hsp70; hsp70RY; HSPH2; RY
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC107571 sequence for NM_002154 edited (data generated by NextGen Sequencing)
  ATGTCGGTGGTGGGCATAGACCTGGGCTTCCAGAGCTGCTACGTCGCTGTGGCCCGCGCC
  GGCGGCATCGAGACTATCGCTAATGAGTATAGCGACCGCTGCACGCCGGCTTGCATTTCT
  TTTGGTCCTAAGAATCGTTCAATTGGAGCAGCAGCTAAAAGCCAGGTAATTTCTAATGCA
  AAGAACACAGTCCAAGGATTTAAAAGATTCCATGGCCGAGCATTCTCTGATCCATTTGTG
  GAGGCAGAAAAATCTAACCTTGCATATGATATTGTGCAGTTGCCTACAGGATTAACAGGT
  ATAAAGGTGACATATATGGAGGAAGAGCGAAATTTTACCACTGAGCAAGTGACTGCCATG
  CTTTTGTCCAAACTGAAGGAGACAGCCGAAAGTGTTCTTAAGAAGCCTGTAGTTGACTGT
  GTTGTTTCGGTTCCTTGTTTCTATACTGATGCAGAAAGACGATCAGTGATGGATACAACA
  CAGATTGCTGGTCTTAATTGCTTGCGATTAATGAATGAAACCACTGCAGTTGCTCTTGCA
  TATGGAATCTATAAGCAGGATCTTCCTGCCTTAGAAGAGAAACCAAGAAATGTAGTTTTT
  GTAGACATGGGCCACTCTGCTTATCAAGTTTCTGTATGTGCATTTAATAGAGGAAAACTG
  AAAGTTCTGGCCACTGCATTTGACACGACATTGGGAGGTAGAAAATTTGATGAAGTGTTA
  GTAAATCACTTCTGTGAAGAATTTGGGAAGAAATACAAGCTAGACATTAAGTCCAAAATC
  CGTGCATTATTACGACTCTCTCAGGAGTGTGAGAAACTCAAGAAATTGATGAGTGCAAAT
  GCTTCAGATCTCCCTTTGAGCATTGAATGTTTTATGAATGATGTTGATGTATCTGGAACT
  ATGAATAGAGGCAAATTTCTGGAGATGTGCAATGATCTCTTAGCTAGAGTGGAGCCACCA
  CTTCGTAGTGTTTTGGAACAAACCAAGTTAAAGAAAGAAGATATTTATGCAGTGGAGATA
  GTTGGTGGTGCTACACGAATCCCTGCGGTAAAAGAGAAGATCAGCAAATTTTTCGGTAAA
  GAACTTAGTACAACATTAAATGCTGATGAAGCTGTCACTCGAGGCTGTGCATTGCAGTGT
  GCCATCTTATCGCCTGCTTTCAAAGTCAGAGAATTTTCTATCACTGATGTAGTACCATAT
  CCAATATCTCTGAGATGGAATTCTCCAGCTGAAGAAGGGTCAAGTGACTGTGAAGTCTTT
  TCCAAAAATCATGCTGCTCCTTTCTCTAAAGTTCTTACATTTTATAGAAAGGAACCTTTC
  ACTCTTGAGGCCTACTACAGCTCTCCTCAGGATTTGCCCTATCCAGATCCTGCTATAGCT
  CAGTTTTCAGTTCAGAAAGTCACTCCTCAGTCTGATGGCTCCAGTTCAAAAGTGAAAGTC
  AAAGTTCGAGTAAATGTCCATGGCATTTTCAGTGTGTCCAGTGCATCTTTAGTGGAGGTT
  CACAAGTCTGAGGAAAATGAGGAGCCAATGGAAACAGATCAGAATGCAAAGGAGGAAGAG
  AAGATGCAAGTGGACCAGGAGGAACCACATGTTGAAGAGCAACAGCAGCAGACACCAGCA
  GAAAATAAGGCAGAGTCTGAAGAAATGGAGACCTCTCAAGCTGGATCCAAGGATAAAAAG
  ATGGACCAACCACCCCAAGCCAAGAAGGCAAAAGTGAAGACCAGTACTGTGGACCTGCCA
  ATCGAGAATCAGCTATTATGGCAGATAGACAGAGAGATGCTCAACTTGTACATTGAAAAT
  GAGGGTAAGATGATCATGCAGGATAAACTGGAGAAGGAGCGGAATGATGCTAAGAACGCA
  GTGGAGGAATATGTGTATGAAATGAGAGACAAGCTTAGTGGTGAATATGAGAAGTTTGTG
  AGTGAAGATGATCGTAACAGTTTTACTTTGAAACTGGAAGATACTGAAAATTGGTTGTAT
  GAGGATGGAGAAGACCAGCCAAAGCAAGTTTATGTTGATAAGTTGGCTGAATTAAAAAAT
  CTAGGTCAACCTATTAAGATACGTTTCCAGGAATCTGAAGAACGACCAAAATTATTTGAA
  GAACTAGGGAAACAGATCCAACAGTATATGAAAATAATCAGCTCTTTCAAAAACAAGGAG
  GACCAGTATGATCATTTGGATGCTGCTGACATGACAAAGGTAGAAAAAAGCACAAATGAA
  GCAATGGAGTGGATGAATAACAAGCTAAATCTGCAGAACAAGCAGAGTTTGACCATGGAT
  CCAGTTGTCAAGTCAAAAGAGATTGAAGCTAAAATTAAGGAGCTGACAAGTACTTGTAGC
  CCTATAATTTCAAAGCCCAAACCCAAAGTGGAACCTCCAAAAGAGGAACAAAAAAATGCA
  GAGCAGAATGGACCAGTGGATGGACAAGGAGACAACCCAGGCCCCCAGGCTGCTGAGCAG
  GGTACAGACACAGCTGTGCCTTCGGATTCAGACAAGAAGCTTCCTGAAATGGACATTGAT
  TGA
  
  Clone variation with respect to NM_002154.3
  475 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002154 unedited
  ATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGCTACCGGCGC
  CTCCTCTGCGGCCACTGAGCCGGAGCCGGCCTGAGCAGCGCTCTCGGTTGCAGTACCCAC
  TGGAAGGACTTAGGCGCTCGCGTGGACACCGCAAGCCCCTCAGTAGCCTCGGCCCAAGAG
  GCCTGCTTTCCACTCGCTAGCCCCGCCGGGGGTCCGTGTCCTGTCTCGGTGGCCGGACCC
  GGGCCCGAGCCCGAGCAGTAGCCGGCGCCATGTCGGTGGTGGGCATAGACCTGGGCTTCC
  AGAGCTGCTACGTCGCTGTGGCCCGCGCCGGCGGCATCGAGACTATCGCTAATGAGTATA
  GCGACCGCTGCACGCCGGCTTGCATTTCTTTTGGTCCTAAGAATCGTTCAATTGGAGCAG
  CAGCTAAAAGCCAGGTAATTTCTAATGCAAAGAACACAGTCCAAGGATTTAAAAGATTCC
  ATGGCCGAGCATTCTCTGATCCATTTGTGGAGGCAGAAAAATCTAACCTTGCATATGATA
  TTGTGCAGTTGCCTACAGGATTAACAGGTATAAAGGTGACATATATGGAGGAAGAGCGAA
  ATTTTACCACTGAGCAAGTGACTGCCATGCTTTTGTCCAAACTGAAGGAGACAGCCGAAA
  GTGTTCTTAAGAAGCCTGTAGTTGACTGTGTTGTTTCGGTTCCTTGTTTCTATACTGATG
  CAGAAAGACGATCAGTGATGGATACAACACAGATTGCTGGTCTTAATTGCTTGCGATAAT
  GAATGACCCACTGCAGTTGCTCTTGCATATGGAATCTNATAGCAGGATCTTCCTGCCTTA
  GAAGAGAAACCAAGAATGTAGTTTTGTAGACATGGN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002154 unedited
  TCCCATGCTTCCCACCCGTGTCTCTGTTCAGGCAAAAGTTATGACCCGCGGCCGCAATCT
  AGAGTCGAGCTTTTTTTTTTTTTTTTTGCTTTATAATAGTCTGTTTTAATAGAAACATGT
  GTTGGAATCAATCAATGTCCATTTCAGGAAGCTTCTTGTCTGAATCCGAAGGCACAGCTG
  TGTCTGTACCCTGCTCAGCAGCCTGGGGGCCTGGGTTGTCTCCTTGTCCATCCACTGGTC
  CATTCTGCTCTGCATTTTTTTGTTCCTCTTTTGGAGGTTCCACTTTGGGTTTGGGCTTTG
  AAATTATAGGGCTACAAGTACTTGTCACCTCCTTAATTTTAGCTTCAATCTCTTTTGACT
  TGACAACTGGATCCATGGTCAAACTCTGCTTGTTCTGCAGATTTAGCTTGTTATTCATCC
  ACTCCATTGCTTCATTTGTGCTTTTTTCTACCTTTGTCATGTCAGCAGCATCCAAATGAT
  CATACTGGTCCTCCTTGTTTTTGAAAGAGCTGATTATTTTCATATACTGTTGGATCTGTT
  TCCCTAGTTCTTCAAATAATTTTGGTCGTTCTTCAGATTCCTGGAAACGTATCTTAATAG
  GTTGACCTAGATTTTTTAATTCAGCCAACTTATCAACATAAACTTGCTTTGGCTGGTCTT
  CTCCATCCTCATANCACCAATTTTCAGTATCTTTCCGTTTCAAAGTAAAACTGTTACGAT
  CATCTTNCCTCCACAACTTTTCTATTTACCACTAAGCTTGTCTCTCTTTTCATACACATA
  TTTCCTCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002154
• Insert Size: 3000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002154.3, NP_002145.3
• RefSeq Size: 3385 bp
• RefSeq ORF: 2523 bp
• Locus ID: 3308
• Cytogenetics: 5q31.1
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS
• Protein Pathways: Antigen processing and presentation
• Gene Summary: ''