MSK1 (RPS6KA5) (NM_004755) Human Tagged ORF Clone

CAT#: RC220636

RPS6KA5 (Myc-DDK-tagged)-Human ribosomal protein S6 kinase, 90kDa, polypeptide 5 (RPS6KA5), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY417768) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220636 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified RPS6KA5 protein (Cat# TP320636). The protein was produced from HEK293T cells transfected with RPS6KA5 cDNA clone (Cat# RC220636) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RPS6KA5
• Synonyms: MSK1; MSPK1; RLPK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220636 representing NM_004755
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAGGAGGGTGGCAGCAGCGGCGGCGCCGCGGGGACCAGCGCGGACGGCGGCGACGGAGGAGAGC
  AGCTCCTCACTGTCAAGCACGAGCTGCGGACTGCTAATTTGACAGGACATGCTGAGAAGGTGGGAATAGA
  AAATTTTGAGCTCCTGAAGGTCCTAGGAACTGGAGCTTATGGAAAAGTATTTCTAGTTCGTAAAATAAGT
  GGCCATGATACTGGAAAGCTGTATGCCATGAAAGTTTTGAAAAAGGCAACAATCGTTCAAAAGGCCAAAA
  CCACAGAGCATACAAGGACAGAACGACAAGTCCTGGAACACATTAGGCAGTCGCCATTTTTGGTAACATT
  ACATTATGCTTTCCAGACAGAAACCAAACTTCATCTCATTTTAGATTATATAAATGGTGGTGAACTTTTT
  ACTCATCTTTCTCAAAGAGAGCGTTTCACAGAGCATGAGGTGCAGATTTATGTTGGAGAGATTGTGCTTG
  CCCTCGAACATCTCCACAAGTTGGGGATTATATATCGTGATATTAAGCTTGAGAATATTCTACTTGATTC
  TAATGGCCATGTGGTGCTGACAGATTTTGGTCTGAGTAAGGAGTTTGTGGCTGATGAAACTGAAAGAGCA
  TATTCCTTTTGTGGAACTATTGAATACATGGCACCAGATATTGTCAGAGGGGGAGATTCAGGACATGACA
  AGGCAGTTGACTGGTGGAGTTTGGGTGTTCTAATGTATGAATTACTAACTGGAGCATCTCCTTTCACTGT
  TGATGGAGAAAAAAATTCCCAAGCTGAGATATCTAGGAGAATATTAAAAAGTGAGCCTCCATATCCCCAA
  GAAATGAGTGCTTTAGCGAAAGACCTAATTCAGCGTCTTTTGATGAAAGATCCCAAGAAGAGATTGGGAT
  GTGGTCCACGTGATGCAGATGAAATCAAAGAACATCTCTTCTTTCAGAAAATAAATTGGGATGATTTAGC
  CGCCAAAAAAGTGCCTGCACCATTTAAGCCAGTCATTCGAGATGAATTAGATGTGAGTAACTTTGCAGAA
  GAGTTCACAGAAATGGATCCCACTTATTCTCCCGCAGCCCTGCCCCAGAGTTCTGAGAAGCTGTTTCAGG
  GCTATTCCTTTGTTGCTCCTTCCATCCTATTCAAGCGTAATGCAGCTGTCATAGACCCTCTTCAGTTTCA
  CATGGGAGTTGAACGTCCTGGAGTGACAAATGTTGCCAGGAGTGCAATGATGAAGGACTCTCCATTCTAT
  CAACACTATGACCTAGATTTGAAGGACAAACCCCTGGGAGAAGGTAGTTTTTCAATTTGTCGAAAGTGTG
  TGCATAAAAAAAGTAACCAAGCTTTTGCAGTCAAAATAATCAGCAAAAGGATGGAAGCCAATACTCAAAA
  GGAAATAACAGCTCTGAAACTCTGTGAAGGACACCCCAATATTGTGAAGTTGCATGAAGTTTTTCATGAT
  CAGCTTCACACGTTTCTAGTGATGGAACTTCTGAATGGAGGAGAACTGTTTGAGCGCATTAAGAAAAAGA
  AGCACTTCAGTGAGACGGAAGCCAGCTACATCATGAGGAAGCTTGTTTCAGCTGTAAGCCACATGCATGA
  TGTTGGAGTGGTGCACAGGGATCTGAAACCTGAGAATTTATTGTTCACCGATGAAAATGACAATTTGGAA
  ATTAAAATAATTGATTTTGGATTTGCACGGCTAAAGCCACCGGATAATCAGCCCCTGAAGACTCCATGCT
  TCACCCTTCATTATGCCGCCCCAGAGCTCTTGAATCAGAACGGCTACGATGAGTCCTGTGACCTGTGGAG
  CTTGGGCGTCATTTTGTACACAATGTTGTCAGGACAGGTTCCCTTCCAATCTCATGACCGAAGTTTGACG
  TGTACCAGCGCGGTGGAAATCATGAAGAAAATTAAAAAGGGAGATTTCTCCTTTGAAGGAGAAGCCTGGA
  AGAATGTATCCCAAGAGGCTAAAGATTTGATCCAAGGACTTCTCACAGTAGATCCAAACAAAAGGCTTAA
  AATGTCTGGCTTGAGGTACAATGAATGGCTACAAGATGGAAGTCAGCTGTCCTCCAATCCTCTGATGACT
  CCGGATATTCTAGGATCTTCCGGAGCTGCCGTGCATACCTGTGTGAAAGCAACCTTCCACGCCTTTAACA
  AATACAAGAGAGAGGGGTTTTGCCTTCAGAATGTTGATAAGGCCCCTTTGGCTAAGAGAAGAAAAATGAA
  AAAGACTAGCACCAGTACCGAGACGCGCAGCAGTTCCAGTGAGAGTTCCCATTCTTCTTCCTCTCATTCT
  CACGGTAAAACTACACCCACCAAGACACTGCAGCCCAGCAATCCTGCCGACAGCAATAACCCGGAGACCC
  TCTTCCAGTTCTCGGACTCAGTAGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220636 representing NM_004755
  Red=Cloning site Green=Tags(s)
  
  MEEEGGSSGGAAGTSADGGDGGEQLLTVKHELRTANLTGHAEKVGIENFELLKVLGTGAYGKVFLVRKIS
  GHDTGKLYAMKVLKKATIVQKAKTTEHTRTERQVLEHIRQSPFLVTLHYAFQTETKLHLILDYINGGELF
  THLSQRERFTEHEVQIYVGEIVLALEHLHKLGIIYRDIKLENILLDSNGHVVLTDFGLSKEFVADETERA
  YSFCGTIEYMAPDIVRGGDSGHDKAVDWWSLGVLMYELLTGASPFTVDGEKNSQAEISRRILKSEPPYPQ
  EMSALAKDLIQRLLMKDPKKRLGCGPRDADEIKEHLFFQKINWDDLAAKKVPAPFKPVIRDELDVSNFAE
  EFTEMDPTYSPAALPQSSEKLFQGYSFVAPSILFKRNAAVIDPLQFHMGVERPGVTNVARSAMMKDSPFY
  QHYDLDLKDKPLGEGSFSICRKCVHKKSNQAFAVKIISKRMEANTQKEITALKLCEGHPNIVKLHEVFHD
  QLHTFLVMELLNGGELFERIKKKKHFSETEASYIMRKLVSAVSHMHDVGVVHRDLKPENLLFTDENDNLE
  IKIIDFGFARLKPPDNQPLKTPCFTLHYAAPELLNQNGYDESCDLWSLGVILYTMLSGQVPFQSHDRSLT
  CTSAVEIMKKIKKGDFSFEGEAWKNVSQEAKDLIQGLLTVDPNKRLKMSGLRYNEWLQDGSQLSSNPLMT
  PDILGSSGAAVHTCVKATFHAFNKYKREGFCLQNVDKAPLAKRRKMKKTSTSTETRSSSSESSHSSSSHS
  HGKTTPTKTLQPSNPADSNNPETLFQFSDSVA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004755
• ORF Size: 2406 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004755.2, NM_004755.3, NP_004746.2
• RefSeq Size: 3883
• RefSeq ORF: 2409
• Locus ID: 9252
• Domains: pkinase, S_TK_X, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors
• Protein Pathways: Bladder cancer, MAPK signaling pathway, Neurotrophin signaling pathway
• MW: 89.7 kDa