MSK1 (RPS6KA5) (NM_004755) Human Untagged Clone

CAT#: SC323666

RPS6KA5 (untagged)-Kinase deficient mutant (K455M) of Human ribosomal protein S6 kinase, 90kDa, polypeptide 5 (RPS6KA5), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPS6KA5
• Synonyms: MSK1; MSPK1; RLPK
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004755, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGGAGGAGGGTGGCAGCAGCGGCGGCGCCGCGGGGACCAGCGCGGACGGCGGCGACGGAGGAGAGC
  AGCTCCTCACTGTCAAGCACGAGCTGCGGACTGCTAATTTGACAGGACATGCTGAGAAGGTGGGAATAGA
  AAATTTTGAGCTCCTGAAGGTCCTAGGAACTGGAGCTTATGGAAAAGTATTTCTAGTTCGTAAAATAAGT
  GGCCATGATACTGGAAAGCTGTATGCCATGAAAGTTTTGAAAAAGGCAACAATCGTTCAAAAGGCCAAAA
  CCACAGAGCATACAAGGACAGAACGACAAGTCCTGGAACACATTAGGCAGTCGCCATTTTTGGTAACATT
  ACATTATGCTTTCCAGACAGAAACCAAACTTCATCTCATTTTAGATTATATAAATGGTGGTGAACTTTTT
  ACTCATCTTTCTCAAAGAGAGCGTTTCACAGAGCATGAGGTGCAGATTTATGTTGGAGAGATTGTGCTTG
  CCCTCGAACATCTCCACAAGTTGGGGATTATATATCGTGATATTAAGCTTGAGAATATTCTACTTGATTC
  TAATGGCCATGTGGTGCTGACAGATTTTGGTCTGAGTAAGGAGTTTGTGGCTGATGAAACTGAAAGAGCA
  TATTCCTTTTGTGGAACTATTGAATACATGGCACCAGATATTGTCAGAGGGGGAGATTCAGGACATGACA
  AGGCAGTTGACTGGTGGAGTTTGGGTGTTCTAATGTATGAATTACTAACTGGAGCATCTCCTTTCACTGT
  TGATGGAGAAAAAAATTCCCAAGCTGAGATATCTAGGAGAATATTAAAAAGTGAGCCTCCATATCCCCAA
  GAAATGAGTGCTTTAGCGAAAGACCTAATTCAGCGTCTTTTGATGAAAGATCCCAAGAAGAGATTGGGAT
  GTGGTCCACGTGATGCAGATGAAATCAAAGAACATCTCTTCTTTCAGAAAATAAATTGGGATGATTTAGC
  CGCCAAAAAAGTGCCTGCACCATTTAAGCCAGTCATTCGAGATGAATTAGATGTGAGTAACTTTGCAGAA
  GAGTTCACAGAAATGGATCCCACTTATTCTCCCGCAGCCCTGCCCCAGAGTTCTGAGAAGCTGTTTCAGG
  GCTATTCCTTTGTTGCTCCTTCCATCCTATTCAAGCGTAATGCAGCTGTCATAGACCCTCTTCAGTTTCA
  CATGGGAGTTGAACGTCCTGGAGTGACAAATGTTGCCAGGAGTGCAATGATGAAGGACTCTCCATTCTAT
  CAACACTATGACCTAGATTTGAAGGACAAACCCCTGGGAGAAGGTAGTTTTTCAATTTGTCGAAAGTGTG
  TGCATAAAAAAAGTAACCAAGCTTTTGCAGTCAAAATAATCAGCAAAAGGATGGAAGCCAATACTCAAAA
  GGAAATAACAGCTCTGAAACTCTGTGAAGGACACCCCAATATTGTGAAGTTGCATGAAGTTTTTCATGAT
  CAGCTTCACACGTTTCTAGTGATGGAACTTCTGAATGGAGGAGAACTGTTTGAGCGCATTAAGAAAAAGA
  AGCACTTCAGTGAGACGGAAGCCAGCTACATCATGAGGAAGCTTGTTTCAGCTGTAAGCCACATGCATGA
  TGTTGGAGTGGTGCACAGGGATCTGAAACCTGAGAATTTATTGTTCACCGATGAAAATGACAATTTGGAA
  ATTAAAATAATTGATTTTGGATTTGCACGGCTAAAGCCACCGGATAATCAGCCCCTGAAGACTCCATGCT
  TCACCCTTCATTATGCCGCCCCAGAGCTCTTGAATCAGAACGGCTACGATGAGTCCTGTGACCTGTGGAG
  CTTGGGCGTCATTTTGTACACAATGTTGTCAGGACAGGTTCCCTTCCAATCTCATGACCGAAGTTTGACG
  TGTACCAGCGCGGTGGAAATCATGAAGAAAATTAAAAAGGGAGATTTCTCCTTTGAAGGAGAAGCCTGGA
  AGAATGTATCCCAAGAGGCTAAAGATTTGATCCAAGGACTTCTCACAGTAGATCCAAACAAAAGGCTTAA
  AATGTCTGGCTTGAGGTACAATGAATGGCTACAAGATGGAAGTCAGCTGTCCTCCAATCCTCTGATGACT
  CCGGATATTCTAGGATCTTCCGGAGCTGCCGTGCATACCTGTGTGAAAGCAACCTTCCACGCCTTTAACA
  AATACAAGAGAGAGGGGTTTTGCCTTCAGAATGTTGATAAGGCCCCTTTGGCTAAGAGAAGAAAAATGAA
  AAAGACTAGCACCAGTACCGAGACGCGCAGCAGTTCCAGTGAGAGTTCCCATTCTTCTTCCTCTCATTCT
  CACGGTAAAACTACACCCACCAAGACACTGCAGCCCAGCAATCCTGCCGACAGCAATAACCCGGAGACCC
  TCTTCCAGTTCTCGGACTCAGTAGCTTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_004755 unedited
  ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCGGGAAAGAGACTCG
  TCTTTGCGTCCGAGTTCTGGAGCCGCCGCACCCCGACTCCTGGGGCCGCGGCAGCGGCTGCGAGGGGACG
  GGCGTCCGCTGTCTCCTGGGTTCCCCTCGTAGCGACCCGCGGGATCGGAAAAAAAGGAGAAGATGGAGGA
  GGAGGGTGGCAGCAGCGGCGGCGCCGCGGGGACCAGCGCGGACGGCGGCGACGGAGGAGAGCAGCTCCTC
  ACTGTCAAGCACGAGCTGCGGACTGCTATTTTGACAGGGACATGCTGAGAAGGTGGGAATAGAAAAATTT
  TGAGCCTCCTGAGGTCCTAGGAACTGGAGCTTATGAAAAAGTATTTCTAGTTTCGTAAAATAAGTGGCCA
  TGATACTGAAAACCTGTATGCATGAAGGTTTGAAAAAGGCACCAATGTTCCAAAGGGCCAAACCCGAACC
  ACCAGGGCCGAACGAACATCTCTGAAACATTTAGCAATGCCCATTTTGGTAACTTACATTGGCTTTCCAA
  AGAACCCAACTTCATCATTTGAGATATAAAGGGTGAACCTTACACTACTTTCAAAGAAAGCTTTCCAGAC
  ATGGTGCGATATATGGGGAAGAGTGGCTGGCTTGAACTTCCAAGTCGGAATTAATCGGATTAGCTGGAAA
  TTCTGGGACTATGCAGCGTCGCACATTCCCCTAAAGAATGGCTGAACTGAGACTCTAGTACTGGATCGCA
  AAATTCAAGG
  
  Kinase domain sequence

  >SC323666 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  ATTGATGMAACCCCTGGGAGAGGTAGTTTTTCATTTGTCGAAAGTGTGTGCATAAAAAAAGTAACCAAGC
  TTTTGCAGTCATGATAATCAGCAAAAGGATGGAAGCCAATACTCAAAAGGAAATAACAGCTCTGAAACTC
  TGTGAAGGACACCCCAATATTGTGAAGTTGCATGAAGTTTTTCATGATCAGCTTCACACGTTTCTAGTGA
  TGGAACTTCTGAATGGAGGAGAACTGTTTGAGCGCATTAAGAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_004755
• ORF Size: 2409 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_004755.2, NP_004746.2
• RefSeq Size: 3883
• RefSeq ORF: 2409
• Locus ID: 9252
• Domains: pkinase, S_TK_X, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors
• Protein Pathways: Bladder cancer, MAPK signaling pathway, Neurotrophin signaling pathway