NOTCH4 (NM_004557) Human Tagged ORF Clone

CAT#: RC221762

  • TrueORF®

NOTCH4 (Myc-DDK-tagged)-Human notch 4 (NOTCH4)


  "NM_004557" in other vectors (3)

Reconstitution Protocol

USD 2,100.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol NOTCH4
Synonyms INT3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC221762 representing NM_004557
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGCCCCCTTCACTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTATGTGTCTCAGTGGTCAGACCCA
GAGGGCTGCTGTGTGGGAGTTTCCCAGAACCCTGTGCCAATGGAGGCACCTGCCTGAGCCTGTCTCTGGG
ACAAGGGACCTGCCAGTGTGCCCCTGGCTTCCTGGGTGAGACGTGCCAGTTTCCTGACCCCTGCCAGAAC
GCCCAGCTCTGCCAAAATGGAGGCAGCTGCCAAGCCCTGCTTCCCGCTCCCCTAGGGCTCCCCAGCTCTC
CCTCTCCATTGACACCCAGCTTCTTGTGCACTTGCCTCCCTGGCTTCACTGGTGAGAGATGCCAGGCCAA
GCTTGAAGACCCTTGTCCTCCCTCCTTCTGTTCCAAAAGGGGCCGCTGCCACATCCAGGCCTCGGGCCGC
CCACAGTGCTCCTGCATGCCTGGATGGACAGGTGAGCAGTGCCAGCTTCGGGACTTCTGTTCAGCCAACC
CATGTGTTAATGGAGGGGTGTGTCTGGCCACATACCCCCAGATCCAGTGCCACTGCCCACCGGGCTTCGA
GGGCCATGCCTGTGAACGTGATGTCAACGAGTGCTTCCAGGACCCAGGACCCTGCCCCAAAGGCACCTCC
TGCCATAACACCCTGGGCTCCTTCCAGTGCCTCTGCCCTGTGGGGCAGGAGGGTCCACGTTGTGAGCTGC
GGGCAGGACCCTGCCCTCCTAGGGGCTGTTCGAATGGGGGCACCTGCCAGCTGATGCCAGAGAAAGACTC
CACCTTTCACCTCTGCCTCTGTCCCCCAGGTTTCATAGGCCCAGACTGTGAGGTGAATCCAGACAACTGT
GTCAGCCACCAGTGTCAGAATGGGGGCACTTGCCAGGATGGGCTGGACACCTACACCTGCCTCTGCCCAG
AAACCTGGACAGGCTGGGACTGCTCCGAAGATGTGGATGAGTGTGAGACCCAGGGTCCCCCTCACTGCAG
AAACGGGGGCACCTGCCAGAACTCTGCTGGTAGCTTTCACTGCGTGTGTGTGAGTGGCTGGGGCGGCACA
AGCTGTGAGGAGAACCTGGATGACTGTATTGCTGCCACCTGTGCCCCGGGATCCACCTGCATTGACCGGG
TGGGCTCTTTCTCCTGCCTCTGCCCACCTGGACGCACAGGACTCCTGTGCCACTTGGAAGACATGTGTCT
GAGCCAGCCGTGCCATGGGGATGCCCAATGCAGCACCAACCCCCTCACAGGCTCCACACTCTGCCTGTGT
CAGCCTGGCTATTCGGGGCCCACCTGCCACCAGGACCTGGACGAGTGTCTGATGGCCCAGCAAGGCCCAA
GTCCCTGTGAACATGGCGGTTCCTGCCTCAACACTCCTGGCTCCTTCAACTGCCTCTGTCCACCTGGCTA
CACAGGCTCCCGTTGTGAGGCTGATCACAATGAGTGCCTCTCCCAGCCCTGCCACCCAGGAAGCACCTGT
CTGGACCTACTTGCCACCTTCCACTGCCTCTGCCCGCCAGGCTTAGAAGGGCAGCTCTGTGAGGTGGAGA
CCAACGAGTGTGCCTCAGCTCCCTGCCTGAACCACGCGGATTGCCATGACCTGCTCAACGGCTTCCAGTG
CATCTGCCTGCCTGGATTCTCCGGCACCCGATGTGAGGAGGATATCGATGAGTGCAGAAGCTCTCCCTGT
GCCAATGGTGGGCAGTGCCAGGACCAGCCTGGAGCCTTCCACTGCAAGTGTCTCCCAGGCTTTGAAGGGC
CACGCTGTCAAACAGAGGTGGATGAGTGCCTGAGTGACCCATGTCCCGTTGGAGCCAGCTGCCTTGATCT
TCCAGGAGCCTTCTTTTGCCTCTGCCCCTCTGGTTTCACAGGCCAGCTCTGTGAGGTTCCCCTGTGTGCT
CCCAACCTGTGCCAGCCCAAGCAGATATGTAAGGACCAGAAAGACAAGGCCAACTGCCTCTGTCCTGATG
GAAGCCCTGGCTGTGCCCCACCTGAGGACAACTGCACCTGCCACCACGGGCACTGCCAGAGATCCTCATG
TGTGTGTGACGTGGGTTGGACGGGGCCAGAGTGTGAGGCAGAGCTAGGGGGCTGCATCTCTGCACCCTGT
GCCCATGGGGGGACCTGCTACCCCCAGCCCTCTGGCTACAACTGCACCTGCCCTACAGGCTACACAGGAC
CCACCTGTAGTGAGGAGATGACAGCTTGTCACTCAGGGCCATGTCTCAATGGCGGCTCCTGCAACCCTAG
CCCTGGAGGCTACTACTGCACCTGCCCTCCAAGCCACACAGGGCCCCAGTGCCAAACCAGCACTGACTAC
TGTGTGTCTGCCCCGTGCTTCAATGGGGGTACCTGTGTGAACAGGCCTGGCACCTTCTCCTGCCTCTGTG
CCATGGGCTTCCAGGGCCCGCGCTGTGAGGGAAAGCTCCGCCCCAGCTGTGCAGACAGCCCCTGTAGGAA
TAGGGCAACCTGCCAGGACAGCCCTCAGGGTCCCCGCTGCCTCTGCCCCACTGGCTACACCGGAGGCAGC
TGCCAGACTCTGATGGACTTATGTGCCCAGAAGCCCTGCCCACGCAATTCCCACTGCCTCCAGACTGGGC
CCTCCTTCCACTGCTTGTGCCTCCAGGGATGGACCGGGCCTCTCTGCAACCTTCCACTGTCCTCCTGCCA
GAAGGCTGCACTGAGCCAAGGCATAGACGTCTCTTCCCTTTGCCACAATGGAGGCCTCTGTGTCGACAGC
GGCCCCTCCTATTTCTGCCACTGCCCCCCTGGATTCCAAGGCAGCCTGTGCCAGGATCACGTGAACCCAT
GTGAGTCCAGGCCTTGCCAGAACGGGGCCACCTGCATGGCCCAGCCCAGTGGGTATCTCTGCCAGTGTGC
CCCAGGCTACGATGGACAGAACTGCTCAAAGGAACTCGATGCTTGTCAGTCCCAACCCTGTCACAACCAT
GGAACCTGTACTCCCAAACCTGGAGGATTCCACTGTGCCTGCCCTCCAGGCTTTGTGGGGCTACGCTGTG
AGGGAGACGTGGACGAGTGTCTGGACCAGCCCTGCCACCCCACAGGCACTGCAGCCTGCCACTCTCTGGC
CAATGCCTTCTACTGCCAGTGTCTGCCTGGACACACAGGCCAGTGGTGTGAGGTGGAGATAGACCCCTGC
CACAGCCAACCCTGCTTTCATGGAGGGACCTGTGAGGCCACAGCAGGATCACCCCTGGGTTTCATCTGCC
ACTGCCCCAAGGGTTTTGAAGGCCCCACCTGCAGCCACAGGGCCCCTTCCTGCGGCTTCCATCACTGCCA
CCACGGAGGCCTGTGTCTGCCCTCCCCTAAGCCAGGCTTCCCACCACGCTGTGCCTGCCTCAGTGGCTAT
GGGGGTCCTGACTGCCTGACCCCACCAGCTCCTAAAGGCTGTGGCCCTCCCTCCCCATGCCTATACAATG
GCAGCTGCTCAGAGACCACGGGCTTGGGGGGCCCAGGCTTTCGATGCTCCTGCCCTCACAGCTCTCCAGG
GCCCCGGTGTCAGAAACCCGGAGCCAAGGGGTGTGAGGGCAGAAGTGGAGATGGGGCCTGCGATGCTGGC
TGCAGTGGCCCGGGAGGAAACTGGGATGGAGGGGACTGCTCTCTGGGAGTCCCAGACCCCTGGAAGGGCT
GCCCCTCCCACTCTCGGTGCTGGCTTCTCTTCCGGGACGGGCAGTGCCACCCACAGTGTGACTCTGAAGA
GTGTCTGTTTGATGGCTACGACTGTGAGACCCCTCCAGCCTGCACTCCAGCCTATGACCAGTACTGCCAT
GATCACTTCCACAACGGGCACTGTGAGAAAGGCTGCAACACTGCAGAGTGTGGCTGGGATGGAGGTGACT
GCAGGCCTGAAGATGGGGACCCAGAGTGGGGGCCCTCCCTGGCCCTGCTGGTGGTACTGAGCCCCCCAGC
CCTAGACCAGCAGCTGTTTGCCCTGGCCCGGGTGCTGTCCCTGACTCTGAGGGTAGGACTCTGGGTAAGG
AAGGATCGTGATGGCAGGGACATGGTGTACCCCTATCCTGGGGCCCGGGCTGAAGAAAAGCTAGGAGGAA
CTCGGGACCCCACCTATCAGGAGAGAGCAGCCCCTCAAACGCAGCCCCTGGGCAAGGAGACCGACTCCCT
CAGTGCTGGGTTTGTGGTGGTCATGGGTGTGGATTTGTCCCGCTGTGGCCCTGACCACCCGGCATCCCGC
TGTCCCTGGGACCCTGGGCTTCTACTCCGCTTCCTTGCTGCGATGGCTGCAGTGGGAGCCCTGGAGCCCC
TGCTGCCTGGACCACTGCTGGCTGTCCACCCTCATGCAGGGACCGCACCCCCTGCCAACCAGCTTCCCTG
GCCTGTGCTGTGCTCCCCAGTGGCCGGGGTGATTCTCCTGGCCCTAGGGGCTCTTCTCGTCCTCCAGCTC
ATCCGGCGTCGACGCCGAGAGCATGGAGCTCTCTGGCTGCCCCCTGGTTTCACTCGACGGCCTCGGACTC
AGTCAGCTCCCCACCGACGCCGGCCCCCACTAGGCGAGGACAGCATTGGTCTCAAGGCACTGAAGCCAAA
GGCAGAAGTTGATGAGGATGGAGTTGTGATGTGCTCAGGCCCTGAGGAGGGAGAGGAGGTGGGCCAGGCT
GAAGAAACAGGCCCACCCTCCACGTGCCAGCTCTGGTCTCTGAGTGGTGGCTGTGGGGCGCTCCCTCAGG
CAGCCATGCTAACTCCTCCCCAGGAATCTGAGATGGAAGCCCCTGACCTGGACACCCGTGGACCTGATGG
GGTGACACCCCTGATGTCAGCAGTTTGCTGTGGGGAAGTACAGTCCGGGACCTTCCAAGGGGCATGGTTG
GGATGTCCTGAGCCCTGGGAACCTCTGCTGGATGGAGGGGCCTGTCCCCAGGCTCACACCGTGGGCACTG
GGGAGACCCCCCTGCACCTGGCTGCCCGATTCTCCCGGCCAACCGCTGCCCGCCGCCTCCTTGAGGCTGG
AGCCAACCCCAACCAGCCAGACCGGGCAGGGCGCACACCCCTTCATGCTGCTGTGGCTGCTGATGCTCGG
GAGGTCTGCCAGCTTCTGCTCCGTAGCAGACAAACTGCAGTGGACGCTCGCACAGAGGACGGGACCACAC
CCTTGATGCTGGCTGCCAGGCTGGCGGTGGAAGACCTGGTTGAAGAACTGATTGCAGCCCAAGCAGACGT
GGGGGCCAGAGATAAATGGGGGAAAACTGCGCTGCACTGGGCTGCTGCCGTGAACAACGCCCGAGCCGCC
CGCTCGCTTCTCCAGGCCGGAGCCGATAAAGATGCCCAGGACAACAGGGAGCAGACGCCGCTATTCCTGG
CGGCGCGGGAAGGAGCGGTGGAAGTAGCCCAGCTACTGCTGGGGCTGGGGGCAGCCCGAGAGCTGCGGGA
CCAGGCTGGGCTAGCGCCGGCGGACGTCGCTCACCAACGTAACCACTGGGATCTGCTGACGCTGCTGGAA
GGGGCTGGGCCACCAGAGGCCCGTCACAAAGCCACGCCGGGCCGCGAGGCTGGGCCCTTCCCGCGCGCAC
GGACGGTGTCAGTAAGCGTGCCCCCGCATGGGGGCGGGGCTCTGCCGCGCTGCCGGACGCTGTCAGCCGG
AGCAGGCCCTCGTGGGGGCGGAGCTTGTCTGCAGGCTCGGACTTGGTCCGTAGACTTGGCTGCGCGGGGG
GGCGGGGCCTATTCTCATTGCCGGAGCCTCTCGGGAGTAGGAGCAGGAGGAGGCCCGACCCCTCGCGGCC
GTAGGTTTTCTGCAGGCATGCGCGGGCCTCGGCCCAACCCTGCGATAATGCGAGGAAGATACGGAGTGGC
TGCCGGGCGCGGAGGCAGGGTCTCAACGGATGACTGGCCCTGTGATTGGGTGGCCCTGGGAGCTTGCGGT
TCTGCCTCCAACATTCCGATCCCGCCTCCTTGCCTTACTCCGTCCCCGGAGCGGGGATCACCTCAACTTG
ACTGTGGTCCCCCAGCCCTCCAAGAAATGCCCATAAACCAAGGAGGAGAGGGTAAAAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC221762 representing NM_004557
Red=Cloning site Green=Tags(s)

MQPPSLLLLLLLLLLLCVSVVRPRGLLCGSFPEPCANGGTCLSLSLGQGTCQCAPGFLGETCQFPDPCQN
AQLCQNGGSCQALLPAPLGLPSSPSPLTPSFLCTCLPGFTGERCQAKLEDPCPPSFCSKRGRCHIQASGR
PQCSCMPGWTGEQCQLRDFCSANPCVNGGVCLATYPQIQCHCPPGFEGHACERDVNECFQDPGPCPKGTS
CHNTLGSFQCLCPVGQEGPRCELRAGPCPPRGCSNGGTCQLMPEKDSTFHLCLCPPGFIGPDCEVNPDNC
VSHQCQNGGTCQDGLDTYTCLCPETWTGWDCSEDVDECETQGPPHCRNGGTCQNSAGSFHCVCVSGWGGT
SCEENLDDCIAATCAPGSTCIDRVGSFSCLCPPGRTGLLCHLEDMCLSQPCHGDAQCSTNPLTGSTLCLC
QPGYSGPTCHQDLDECLMAQQGPSPCEHGGSCLNTPGSFNCLCPPGYTGSRCEADHNECLSQPCHPGSTC
LDLLATFHCLCPPGLEGQLCEVETNECASAPCLNHADCHDLLNGFQCICLPGFSGTRCEEDIDECRSSPC
ANGGQCQDQPGAFHCKCLPGFEGPRCQTEVDECLSDPCPVGASCLDLPGAFFCLCPSGFTGQLCEVPLCA
PNLCQPKQICKDQKDKANCLCPDGSPGCAPPEDNCTCHHGHCQRSSCVCDVGWTGPECEAELGGCISAPC
AHGGTCYPQPSGYNCTCPTGYTGPTCSEEMTACHSGPCLNGGSCNPSPGGYYCTCPPSHTGPQCQTSTDY
CVSAPCFNGGTCVNRPGTFSCLCAMGFQGPRCEGKLRPSCADSPCRNRATCQDSPQGPRCLCPTGYTGGS
CQTLMDLCAQKPCPRNSHCLQTGPSFHCLCLQGWTGPLCNLPLSSCQKAALSQGIDVSSLCHNGGLCVDS
GPSYFCHCPPGFQGSLCQDHVNPCESRPCQNGATCMAQPSGYLCQCAPGYDGQNCSKELDACQSQPCHNH
GTCTPKPGGFHCACPPGFVGLRCEGDVDECLDQPCHPTGTAACHSLANAFYCQCLPGHTGQWCEVEIDPC
HSQPCFHGGTCEATAGSPLGFICHCPKGFEGPTCSHRAPSCGFHHCHHGGLCLPSPKPGFPPRCACLSGY
GGPDCLTPPAPKGCGPPSPCLYNGSCSETTGLGGPGFRCSCPHSSPGPRCQKPGAKGCEGRSGDGACDAG
CSGPGGNWDGGDCSLGVPDPWKGCPSHSRCWLLFRDGQCHPQCDSEECLFDGYDCETPPACTPAYDQYCH
DHFHNGHCEKGCNTAECGWDGGDCRPEDGDPEWGPSLALLVVLSPPALDQQLFALARVLSLTLRVGLWVR
KDRDGRDMVYPYPGARAEEKLGGTRDPTYQERAAPQTQPLGKETDSLSAGFVVVMGVDLSRCGPDHPASR
CPWDPGLLLRFLAAMAAVGALEPLLPGPLLAVHPHAGTAPPANQLPWPVLCSPVAGVILLALGALLVLQL
IRRRRREHGALWLPPGFTRRPRTQSAPHRRRPPLGEDSIGLKALKPKAEVDEDGVVMCSGPEEGEEVGQA
EETGPPSTCQLWSLSGGCGALPQAAMLTPPQESEMEAPDLDTRGPDGVTPLMSAVCCGEVQSGTFQGAWL
GCPEPWEPLLDGGACPQAHTVGTGETPLHLAARFSRPTAARRLLEAGANPNQPDRAGRTPLHAAVAADAR
EVCQLLLRSRQTAVDARTEDGTTPLMLAARLAVEDLVEELIAAQADVGARDKWGKTALHWAAAVNNARAA
RSLLQAGADKDAQDNREQTPLFLAAREGAVEVAQLLLGLGAARELRDQAGLAPADVAHQRNHWDLLTLLE
GAGPPEARHKATPGREAGPFPRARTVSVSVPPHGGGALPRCRTLSAGAGPRGGGACLQARTWSVDLAARG
GGAYSHCRSLSGVGAGGGPTPRGRRFSAGMRGPRPNPAIMRGRYGVAAGRGGRVSTDDWPCDWVALGACG
SASNIPIPPPCLTPSPERGSPQLDCGPPALQEMPINQGGEGKK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_004557
ORF Size 6009 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004557.1, NM_004557.2, NM_004557.3, NP_004548.3
RefSeq Size 6762 bp
RefSeq ORF 6012 bp
Locus ID 4855
Cytogenetics 6p21.32
Protein Families Druggable Genome
Protein Pathways Dorso-ventral axis formation, Notch signaling pathway
MW 210.1 kDa
Gene Summary 'This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor may play a role in vascular, renal and hepatic development. Mutations in this gene may be associated with schizophrenia. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]'

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