NOTCH4 (NM_004557) Human Untagged Clone

CAT#: SC303498

NOTCH4 (untagged)-Human notch 4 (NOTCH4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NOTCH4
• Synonyms: INT3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004557 edited
  CAGGCCGAGGAGGAAGAAGAGGGGCAGTGGGAGCAGAGGAGGTGGCTCCTGCCCCAGTGA
  GAGCTCTGAGGGTCCCTGCCTGAAGAGGGACAGGGACTGGGGCTTGGAGAAGGGGCTGTG
  GAATGCAGCCCCCTTCACTGCTGCTGCTGCTGCTGCTGCTGCTGCTATGTGTCTCAGTGG
  TCAGACCCAGAGGGCTGCTGTGTGGGAGTTTCCCAGAACCCTGTGCCAATGGAGGCACCT
  GCCTGAGCCTGTCTCTGGGACAAGGGACCTGCCAGTGTGCCCCTGGCTTCCTGGGTGAGA
  CGTGCCAGTTTCCTGACCCCTGCCAGAACGCCCAGCTCTGCCAAAATGGAGGCAGCTGCC
  AAGCCCTGCTTCCCGCTCCCCTAGGGCTCCCCAGCTCTCCCTCTCCATTGACACCCAGCT
  TCTTGTGCACTTGCCTCCCTGGCTTCACTGGCGAGAGATGCCAGGCCCAGCTTGAAGACC
  CTTGTCCTCCCTCCTTCTGTTCCAAAAGGGGCCGCTGCCACATCCAGGCCTCGGGCCGCC
  CACAGTGCTCCTGCATGCCTGGATGGACAGGTGAGCAGTGCCAGCTTCGGGACTTCTGTT
  CAGCCAACCCATGTGTTAATGGAGGGGTGTGTCTGGCCACATACCCCCAGATCCAGTGCC
  ACTGCCCACCGGGCTTCGAGGGCCATGCCTGTGAACGTGATGTCAACGAGTGCTTCCAGG
  ACCCAGGACCCTGCCCCAAAGGCACCTCCTGCCATAACACCCTGGGCTCCTTCCAGTGCC
  TCTGCCCTGTGGGGCAGGAGGGTCCACGTTGTGAGCTGCGGGCAGGACCCTGCCCTCCTA
  GGGGCTGTTCGAATGGGGGCACCTGCCAGCTGATGCCAGAGAAAGACTCCACCTTTCACC
  TCTGCCTCTGTCCCCCAGGTTTCATAGGCCCAGACTGTGAGGTGAATCCAGACAACTGTG
  TCAGCCACCAGTGTCAGAATGGGGGCACTTGCCAGGATGGGCTGGACACCTACACCTGCC
  TCTGCCCAGAAACCTGGACAGGCTGGGACTGCTCCGAAGATGTGGATGAGTGTGAGACCC
  AGGGTCCCCCTCACTGCAGAAACGGGGGCACCTGCCAGAACTCTGCTGGTAGCTTTCACT
  GCGTGTGTGTGAGTGGCTGGGGCGGCACAAGCTGTGAGGAGAACCTGGATGACTGTATTG
  CTGCCACCTGTGCCCCGGGATCCACCTGCATTGACCGGGTGGGCTCTTTCTCCTGCCTCT
  GCCCACCTGGACGCACAGGACTCCTGTGCCACTTGGAAGACATGTGTCTGAGCCAGCCGT
  GCCATGGGGATGCCCAATGCAGCACCAACCCCCTCACAGGCTCCACACTCTGCCTGTGTC
  AGCCTGGCTATTCGGGGCCCACCTGCCACCAGGACCTGGACGAGTGTCTGATGGCCCAGC
  AAGGCCCAAGTCCCTGTGAACATGGCGGTTCCTGCCTCAACACTCCTGGCTCCTTCAACT
  GCCTCTGTCCACCTGGCTACACAGGCTCCCGTTGTGAGGCTGATCACAATGAGTGCCTCT
  CCCAGCCCTGCCACCCAGGAAGCACCTGTCTGGACCTACTTGCCACCTTCCACTGCCTCT
  GCCCGCCAGGCTTAGAAGGGCAGCTCTGTGAGGTGGAGACCAACGAGTGTGCCTCAGCTC
  CCTGCCTGAACCACGCGGATTGCCATGACCTGCTCAACGGCTTCCAGTGTATCTGCCTGC
  CTGGATTCTCCGGCACCCGATGTGAGGAGGATATCGATGAGTGCAGAAGCTCTCCCTGTG
  CCAATGGTGGGCAGTGCCAGGACCAGCCTGGAGCCTTCCACTGCAAGTGTCTCCCAGGCT
  TTGAAGGGCCACGCTGTCAAACAGAGGTGGATGAGTGCCTGAGTGACCCATGTCCCGTTG
  GAGCCAGCTGCCTTGATCTTCCAGGAGCCTTCTTTTGCCTCTGCCCCTCTGGTTTCACAG
  GCCAGCTCTGTGAGGTTCCCCTGTGTGCTCCCAACCTGTGCCAGCCCAAGCAGATATGTA
  AGGACCAGAAAGACAAGGCCAACTGCCTCTGTCCTGATGGAAGCCCTGGCTGTGCCCCAC
  CTGAGGACAACTGCACCTGCCACCACGGGCACTGCCAGAGATCCTCATGTGTGTGTGACG
  TGGGTTGGACGGGGCCAGAGTGTGAGGCAGAGCTAGGGGGCTGCATCTCTGCACCCTGTG
  CCCATGGGGGGACCTGCTACCCCCAGCCCTCTGGCTACAACTGCACCTGCCCTACAGGCT
  ACACAGGACCCACCTGTAGTGAGGAGATGACAGCTTGTCACTCAGGGCCATGTCTCAATG
  GCGGCTCCTGCAACCCTAGCCCTGGAGGCTACTACTGCACCTGCCCTCCAAGCCACACAG
  GGCCCCAGTGCCAAACCAGCACTGACTACTGTGTGTCTGCCCCGTGCTTCAATGGGGGTA
  CCTGTGTGAACAGGCCTGGCACCTTCTCCTGCCTCTGTGCCATGGGCTTCCAGGGCCCGC
  GCTGTGAGGGAAAGCTCCGCCCCAGCTGTGCAGACAGCCCCTGTAGGAATAGGGCAACCT
  GCCAGGACAGCCCTCAGGGTCCCCGCTGCCTCTGCCCCACTGGCTACACCGGAGGCAGCT
  GCCAGACTCTGATGGACTTATGTGCCCAGAAGCCCTGCCCACGCAATTCCCACTGCCTCC
  AGACTGGGCCCTCCTTCCACTGCTTGTGCCTCCAGGGATGGACCGGGCCTCTCTGCAACC
  TTCCACTGTCCTCCTGCCAGAAGGCTGCACTGAGCCAAGGCATAGACGTCTCTTCCCTTT
  GCCACAATGGAGGCCTCTGTGTCGACAGCGGCCCCTCCTATTTCTGCCACTGCCCCCCTG
  GATTCCAAGGCAGCCTGTGCCAGGATCACGTGAACCCATGTGAGTCCAGGCCTTGCCAGA
  ACGGGGCCACCTGCATGGCCCAGCCCAGTGGGTATCTCTGCCAGTGTGCCCCAGGCTACG
  ATGGACAGAACTGCTCAAAGGAACTCGATGCTTGTCAGTCCCAACCCTGTCACAACCATG
  GAACCTGTACTCCCAAACCTGGAGGCTTCCACTGTGCCTGCCCTCCAGGCTTTGTGGGGC
  TACGCTGTGAGGGAGACGTGGACGAGTGTCTGGACCAGCCCTGCCACCCCACAGGCACTG
  CAGCCTGCCACTCTCTGGCCAATGCCTTCTACTGCCAGTGTCTGCCTGGACACACAGGCC
  AGTGGTGTGAGGTGGAGATAGACCCCTGCCACAGCCAACCCTGCTTTCATGGAGGGACCT
  GTGAGGCCACAGCAGGATCACCCCTGGGTTTCATCTGCCACTGCCCCAAGGGTTTTGAAG
  GCCCCACCTGCAGCCACAGGGCCCCTTCCTGCGGCTTCCATCACTGCCACCACGGAGGCC
  TGTGTCTGCCCTCCCCTAAGCCAGGCTTCCCACCACGCTGTGCCTGCCTCAGTGGCTATG
  GGGGTCCTGACTGCCTGACCCCACCAGCTCCTAAAGGCTGTGGCCCTCCCTCCCCATGCC
  TATACAATGGCAGCTGCTCAGAGACCACGGGCTTGGGGGGCCCAGGCTTTCGATGCTCCT
  GCCCTCACAGCTCTCCAGGGCCCCGGTGTCAGAAACCCGGAGCCAAGGGGTGTGAGGGCA
  GAAGTGGAGATGGGGCCTGCGATGCTGGCTGCAGTGGCCCGGGAGGAAACTGGGATGGAG
  GGGACTGCTCTCTGGGAGTCCCAGACCCCTGGAAGGGCTGCCCCTCCCACTCTCGGTGCT
  GGCTTCTCTTCCGGGACGGGCAGTGCCACCCACAGTGTGACTCTGAAGAGTGTCTGTTTG
  ATGGCTACGACTGTGAGACCCCTCCAGCCTGCACTCCAGCCTATGACCAGTACTGCCATG
  ATCACTTCCACAACGGGCACTGTGAGAAAGGCTGCAACACTGCAGAGTGTGGCTGGGATG
  GAGGTGACTGCAGGCCTGAAGATGGGGACCCAGAGTGGGGGCCCTCCCTGGCCCTGCTGG
  TGGTACTGAGCCCCCCAGCCCTAGACCAGCAGCTGTTTGCCCTGGCCCGGGTGCTGTCCC
  TGACTCTGAGGGTAGGACTCTGGGTAAGGAAGGATCGTGATGGCAGGGACATGGTGTACC
  CCTATCCTGGGGCCCGGGCTGAAGAAAAGCTAGGAGGAACTCGGGACCCCACCTATCAGG
  AGAGAGCAGCCCCTCAAACGCAGCCCCTGGGCAAGGAGACCGACTCCCTCAGTGCTGGGT
  TTGTGGTGGTCATGGGTGTGGATTTGTCCCGCTGTGGCCCTGACCACCCGGCATCCCGCT
  GTCCCTGGGACCCTGGGCTTCTACTCCGCTTCCTTGCTGCGATGGCTGCAGTGGGAGCCC
  TGGAGCCCCTGCTGCCTGGACCACTGCTGGCTGTCCACCCTCATGCAGGGACCGCACCCC
  CTGCCAACCAGCTTCCCTGGCCTGTGCTGTGCTCCCCAGTGGCCGGGGTGATTCTCCTGG
  CCCTAGGGGCTCTTCTCGTCCTCCAGCTCATCCGGCGTCGACGCCGAGAGCATGGAGCTC
  TCTGGCTGCCCCCTGGTTTCACTCGACGGCCTCGGACTCAGTCAGCTCCCCACCGACGCC
  GGCCCCCACTAGGCGAGGACAGCATTGGTCTCAAGGCACTGAAGCCAAAGGCAGAAGTTG
  ATGAGGATGGAGTTGTGATGTGCTCAGGCCCTGAGGAGGGAGAGGAGGCTGAAGAAACAG
  GCCCACCCTCCACGTGCCAGCTCTGGTCTCTGAGTGGTGGCTGTGGGGCGCTCCCTCAGG
  CAGCCATGCTAACTCCTCCCCAGGAATCTGAGATGGAAGCCCCTGACCTGGACACCCGTG
  GACCTGATGGGGTGACACCCCTGATGTCAGCAGTTTGCTGTGGGGAAGTACAGTCCGGGA
  CCTTCCAAGGGGCATGGTTGGGATGTCCTGAGCCCTGGGAACCTCTGCTGGATGGAGGGG
  CCTGTCCCCAGGCTCACACCGTGGGCACTGGGGAGACCCCCCTGCACCTGGCTGCCCGAT
  TCTCCCGGCCAACCGCTGCCCGCCGCCTCCTTGAGGCTGGAGCCAACCCCAACCAGCCAG
  ACCGGGCAGGGCGCACACCCCTTCATGCTGCTGTGGCTGCTGATGCTCGGGAGGTCTGCC
  AGCTTCTGCTCCGTAGCAGACAAACTGCAGTGGACGCTCGCACAGAGGACGGGACCACAC
  CCTTGATGCTGGCTGCCAGGCTGGCGGTGGAAGACCTGGTTGAAGAACTGATTGCAGCCC
  AAGCAGACGTGGGGGCCAGAGATAAATGGGGGAAAACTGCGCTGCACTGGGCTGCTGCCG
  TGAACAACGCCCGAGCCGCCCGCTCGCTTCTCCAGGCCGGAGCCGATAAAGATGCCCAGG
  ACAACAGGGAGCAGACGCCGCTATTCCTGGCGGCGCGGGAAGGAGCGGTGGAAGTAGCCC
  AGCTACTGCTGGGGCTGGGGGCAGCCCGAGAGCTGCGGGACCAGGCTGGGCTAGCGCCGG
  CGGACGTCGCTCACCAACGTAACCACTGGGATCTGCTGACGCTGCTGGAAGGGGCTGGGC
  CACCAGAGGCCCGTCACAAAGCCACGCCGGGCCGCGAGGCTGGGCCCTTCCCGCGCGCAC
  GGACGGTGTCAGTAAGCGTGCCCCCGCATGGGGGCGGGGCTCTGCCGCGCTGCCGGACGC
  TGTCAGCCGGAGCAGGCCCTCGTGGGGGCGGAGCTTGTCTGCAGGCTCGGACTTGGTCCG
  TAGACTTGGCTGCGCGGGGGGGCGGGGCCTATTCTCATTGCCGGAGCCTCTCGGGAGTAG
  GAGCAGGAGGAGGCCCGACCCCTCGCGGCCGTAGGTTTTCTGCAGGCATGCGCGGGCCTC
  GGCCCAACCCTGCGATAATGCGAGGAAGATACGGAGTGGCTGCCGGGCGCGGAGGCAGGG
  TCTCAACGGATGACTGGCCCTGTGATTGGGTGGCCCTGGGAGCTTGCGGTTCTGCCTCCA
  ACATTCCGATCCCGCCTCCTTGCCTTACTCCGTCCCCGGAGCGGGGATCACCTCAACTTG
  ACTGTGGTCCCCCAGCCCTCCAAGAAATGCCCATAAACCAAGGAGGAGAGGGTAAAAAAT
  AGAAGAATACATGGTAGGGAGGAATTCCAAAAATGATTACCCATTAAAAGGCAGGCTGGA
  AGGCCTTCCTGGTTTTAAGATGGATCCCCCAAAATGAAGGGTTGTGAGTTTAGTTTCTCT
  CCTAAAATGAATGTATGCCCACCAGAGCAGACATCTTCCACGTGGAGAAGCTGCAGCTCT
  GGAAAGAGGGTTTAAGATGCTAGGATGAGGCAGGCCCAGTCCTCCTCCAGAAAATAAGAC
  AGGCCACAGGAGGGCAGAGTGGAGTGGAAATACCCCTAAGTTGGAACCAAGAATTGCAGG
  CATATGGGATGTAAGATGTTCTTTCCTATATATGGTTTCCAAAGGGTGCCCCTATGATCC
  ATTGTCCCCACTGCCCACAAATGGCTGACAAATATTTATTGGGCACCTACTATGTGCCAG
  GCACTGTGTAGGTGCTGAAAAGTGGCCAAGGGCCACCCCCGCTGATGACTCCTTGCATTC
  CCTCCCCTCACAACAAAGAACTCCACTGTGGGGATGAAGCGCTTCTTCTAGCCACTGCTA
  TCGCTATTTAAGAACCCTAAATCTGTCACCCATAATAAAGCTGATTTGAAGTGTTAAAAA
  AAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004557 unedited
  TTTTGTCCCGTTCACATTTGTAATACCACNCACTATAGGGCGGCCGCGGAATTCGCACCA
  GGCAGGCCGAGGAGGAAGAAGAGGGCAGTGGGAGCAGAGGAGGTGGCTCCTGCCCCAGTG
  AGAGCTCTGAGGGTCCCTGCCTGAAGAGGGACAGGGACTGGGGCTTGGAGAAGGGGCTGT
  GGAATGCAGCCCCCTTCACTGCTGCTGCTGCTGCTGCTGCTGCTGCTATGTGTCTCAGTG
  GTCAGACCCAGAGGGCTGCTGTGTGGGAGTTTCCCAGAACCCTGTGCCAATGGAGGCACC
  TGCCTGAGCCTGTCTCTGGGACAAGGGACCTGCCAGTGTGCCCCTGGCTTCCTGGGTGAG
  ACGTGCCAGTTTCCTGACCCCTGCCAGAACGCCCAGCTCTGCCAAAATGGAGGCAGCTGC
  CAAGCCCTGCTTCCCGCTCCCCTAGGGCTCCCCAGCTCTCCCTCTCCATTGACACCCAGC
  TTCTTGTGCACTTGCCTCCCTGGCTTCACTGGCGAGAGATGCCAGGCCCAGCTTGAAGAC
  CCTTGTCCTCCCTCCTTCTGTTCCAAAAGGGGCCGCTGCCACATCCAGGCCTCGGGCCGC
  CCACAGTGCTCCTGCATGCCTGGATGGACAGGTGAGCAGTGCCAGCTTCGGGACTTCTGT
  TCAGCCAACCCATGTGTTAATGGAGGGGTGTGTCTGGCCACATACCCCCAGATCCAGTGC
  CACTGCCCACCCGGCTTCCAGGGCCATGCCTGTGAACGTGATGTCAACGAGTGCTTCCAG
  GACCCATGACCCTGCCCCCATGGCACCTCCTGCCATAACACCCTGGGCTCCTTCCAGTGC
  CTCTGCCCTGTGGGGCAAGACGGTCCAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004557 unedited
  TTNCCCGGCCTTGGTGATGGCACTTCCCAGGNCCAGNGNGAGCACTGGGGNAGGGTCACA
  GGNTGCCACCCGGGNATCTGTTCAGNAAACAGCTATGACCGCGGCCGCAATCTAGAGTCG
  AGTTTTTTTTTTTTTTTTTTAACACTTCAAATCAGCTTTATTATGGGTGACAGATTTAGG
  GTTCTTAAATAGCGATAGCAGTGGCTAGAAGAAGCGCTTCATCCCCACAGTGGAGTTCTT
  TGTTGTGAGGGGAGGGAATGCAAGGAGTCATCAGCGGGGGTGGCCCTTGGCCACTTTTCA
  GCACCTACACAGTGCCTGGCACATAGTAGGTGCCCAATAAATATTTGTCAGCCATTTGTG
  GGCAGTGGGGACAATGGATCATAGGGGCACCCTTTGGAAACCATATATAGGAAAGAACAT
  CTTACATCCCATATGCCTGCAATTCTTGGTTCCAACTTAGGGGTATTTCCACTCCACTCT
  GCCCTCCTGTGGCCTGTCTTATTTTCTGGAGGAGGACTGGGCCTGCCTCATCCTAGCATC
  TTAAACCCTCTTTCCAGAGCTGCAGCTTCTCCACGTGGAAGATGTCTGCTCTGGTGGGCA
  TACATTCATTTTAGGAGAGAAACTAAACTCACAACCCTTCATTTTGGGGGATCCATCTTA
  AAACCAGGAAGGCCTTCCAGCCTGCCTTTTAATGGGTAATCATTTTTGGAATTCCTCCCT
  ACCATGTATTCTTCTATTTTTTACCCTCTCCTCCTTGGTTTATGGGCATTTCTTGGGAGG
  CTGGGGGACCACAGTCAAGTTGAGGTGATCCCCGCTCCGGGGACGGAGTAAGGCAAGGNA
  GCGGGATCCGGATGTNTGNAGCAGAACCCGCAGCTCCCAGGA
  
• Restriction Sites: Please inquire
• ACCN: NM_004557
• Insert Size: 6700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: There are 17 nucleotide differences between the OriGene clone and the NCBI reference ORF. OriGene considers these to be polymorphisms and to reflect the natural differences between individuals. These result in the substitution of 5 amino acid ( 4 deletion and 1sub).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004557.3, NP_004548.3
• RefSeq Size: 6762 bp
• RefSeq ORF: 6012 bp
• Locus ID: 4855
• Cytogenetics: 6p21.32
• Protein Families: Druggable Genome
• Protein Pathways: Dorso-ventral axis formation, Notch signaling pathway
• Gene Summary: 'This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor may play a role in vascular, renal and hepatic development. Mutations in this gene may be associated with schizophrenia. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the protein.