GTF2A1 (NM_201595) Human Tagged ORF Clone

CAT#: RC221859

GTF2A1 (Myc-DDK-tagged)-Human general transcription factor IIA, 1, 19/37kDa (GTF2A1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GTF2A1
• Synonyms: TF2A1; TFIIA; TFIIA-42; TFIIAL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221859 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAACTCGGCAAATACAAACACCGTGCCTAAATTATACAGATCTGTGATTGAAGATGTCATTAATG
  ATGTGAGAGACATCTTTCTGGATGATGGAGTGGATGAACAAGTACTGATGGAACTAAAAACTTTATGGGA
  AAACAAACTAATGCAGTCCAGGGCAGTAGATGGATTTCATTCAGAAGAGCAGCAGCTTCTACTGCAAGTT
  CAACAGCAGCATCAACCCCAGCAGCAGCAGCATCACCACCATCACCATCATCAGCAAGCTCAGCCTCAGC
  AGACAGTACCTCAGCAAGCGCAGACCCAGCAGGTTCTTATTCCTGCATCACAGCAAGCCACAGCACCACA
  AGTTATTGTTCCAGATTCTAAGTTGATACAGCATATGAATGCATCAAACATGAGTGCTGCTGCTACAGCT
  GCTACCTTAGCACTCCCTGCAGGTGTGACTCCTGTTCAGCAGATATTAACAAATTCAGGCCAGCTTCTTC
  AGGTGGTCAGAGCAGCCAATGGTGCCCAATATATCTTTCAGCCTCAGCAGTCAGTGGTTCTACAACAACA
  GGTTATACCACAAATGCAGCCTGGTGGAGTACAAGCTCCTGTTATACAGCAGGTGCTGGCTCCTCTTCCT
  GGAGGGATTTCACCACAGACAGGTGTCATCATCCAGCCTCAGCAAATCTTATTTACAGGAAATAAGACTC
  AAGTTATACCTACGACAGTGGCAGCACCTACACCAGCCCAAGCACAGATAACTGCAACTGGCCAGCAGCA
  ACCGCAGGCCCAGCCTGCTCAAACACAAGCTCCATTGGTCTTACAAGTTGATGGAACTGGGGATACATCA
  TCTGAAGAAGATGAAGATGAAGAAGAAGACTATGATGATGATGAGGAGGAAGACAAAGAGAAAGATGGAG
  CTGAAGATGGGCAGGTGGAAGAAGAGCCCCTCAATAGTGAAGATGATGTGAGTGATGAGGAAGGACAGGA
  ACTCTTTGACACAGAAAATGTTGTTGTATGCCAATATGATAAGATACACAGAAGTAAAAACAAATGGAAA
  TTTCATCTCAAGGATGGCATTATGAATCTTAATGGAAGAGATTATATATTTTCCAAAGCCATTGGAGATG
  CAGAATGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221859 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MANSANTNTVPKLYRSVIEDVINDVRDIFLDDGVDEQVLMELKTLWENKLMQSRAVDGFHSEEQQLLLQV
  QQQHQPQQQQHHHHHHHQQAQPQQTVPQQAQTQQVLIPASQQATAPQVIVPDSKLIQHMNASNMSAAATA
  ATLALPAGVTPVQQILTNSGQLLQVVRAANGAQYIFQPQQSVVLQQQVIPQMQPGGVQAPVIQQVLAPLP
  GGISPQTGVIIQPQQILFTGNKTQVIPTTVAAPTPAQAQITATGQQQPQAQPAQTQAPLVLQVDGTGDTS
  SEEDEDEEEDYDDDEEEDKEKDGAEDGQVEEEPLNSEDDVSDEEGQELFDTENVVVCQYDKIHRSKNKWK
  FHLKDGIMNLNGRDYIFSKAIGDAEW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_201595
• ORF Size: 1131 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_201595.1, NM_201595.2, NP_963889.1
• RefSeq Size: 5956 bp
• RefSeq ORF: 1014 bp
• Locus ID: 2957
• Cytogenetics: 14q31.1
• Protein Families: Transcription Factors
• Protein Pathways: Basal transcription factors
• MW: 41.5 kDa
• Gene Summary: 'Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and several general initiation factors (summarized by DeJong and Roeder, 1993 [PubMed 8224848]). One of these factors is TFIIA, which when purified from HeLa extracts consists of 35-, 19-, and 12-kD subunits.[supplied by OMIM, Jul 2010]'