GTF2A1 (NM_201595) Human Tagged ORF Clone

CAT#: RG221859

GTF2A1 (GFP-tagged) - Human general transcription factor IIA, 1, 19/37kDa (GTF2A1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GTF2A1
• Synonyms: TF2A1; TFIIA; TFIIA-42; TFIIAL
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221859 representing NM_201595
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACTAAAAACTTTATGGGAAAACAAACTAATGCAGTCCAGGGCAGTAGATGGATTTCATTCAGAAG
  AGCAGCAGCTTCTACTGCAAGTTCAACAGCAGCATCAACCCCAGCAGCAGCAGCATCACCACCATCACCA
  TCATCAGCAAGCTCAGCCTCAGCAGACAGTACCTCAGCAAGCGCAGACCCAGCAGGTTCTTATTCCTGCA
  TCACAGCAAGCCACAGCACCACAAGTTATTGTTCCAGATTCTAAGTTGATACAGCATATGAATGCATCAA
  ACATGAGTGCTGCTGCTACAGCTGCTACCTTAGCACTCCCTGCAGGTGTGACTCCTGTTCAGCAGATATT
  AACAAATTCAGGCCAGCTTCTTCAGGTGGTCAGAGCAGCCAATGGTGCCCAATATATCTTTCAGCCTCAG
  CAGTCAGTGGTTCTACAACAACAGGTTATACCACAAATGCAGCCTGGTGGAGTACAAGCTCCTGTTATAC
  AGCAGGTGCTGGCTCCTCTTCCTGGAGGGATTTCACCACAGACAGGTGTCATCATCCAGCCTCAGCAAAT
  CTTATTTACAGGAAATAAGACTCAAGTTATACCTACGACAGTGGCAGCACCTACACCAGCCCAAGCACAG
  ATAACTGCAACTGGCCAGCAGCAACCGCAGGCCCAGCCTGCTCAAACACAAGCTCCATTGGTCTTACAAG
  TTGATGGAACTGGGGATACATCATCTGAAGAAGATGAAGATGAAGAAGAAGACTATGATGATGATGAGGA
  GGAAGACAAAGAGAAAGATGGAGCTGAAGATGGGCAGGTGGAAGAAGAGCCCCTCAATAGTGAAGATGAT
  GTGAGTGATGAGGAAGGACAGGAACTCTTTGACACAGAAAATGTTGTTGTATGCCAATATGATAAGATAC
  ACAGAAGTAAAAACAAATGGAAATTTCATCTCAAGGATGGCATTATGAATCTTAATGGAAGAGATTATAT
  ATTTTCCAAAGCCATTGGAGATGCAGAATGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221859 representing NM_201595
  Red=Cloning site Green=Tags(s)
  
  MELKTLWENKLMQSRAVDGFHSEEQQLLLQVQQQHQPQQQQHHHHHHHQQAQPQQTVPQQAQTQQVLIPA
  SQQATAPQVIVPDSKLIQHMNASNMSAAATAATLALPAGVTPVQQILTNSGQLLQVVRAANGAQYIFQPQ
  QSVVLQQQVIPQMQPGGVQAPVIQQVLAPLPGGISPQTGVIIQPQQILFTGNKTQVIPTTVAAPTPAQAQ
  ITATGQQQPQAQPAQTQAPLVLQVDGTGDTSSEEDEDEEEDYDDDEEEDKEKDGAEDGQVEEEPLNSEDD
  VSDEEGQELFDTENVVVCQYDKIHRSKNKWKFHLKDGIMNLNGRDYIFSKAIGDAEW
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_201595
• ORF Size: 1011 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_201595.1, NP_963889.1
• RefSeq Size: 1358 bp
• RefSeq ORF: 1014 bp
• Locus ID: 2957
• Cytogenetics: 14q31.1
• Protein Families: Transcription Factors
• Protein Pathways: Basal transcription factors
• Gene Summary: 'Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and several general initiation factors (summarized by DeJong and Roeder, 1993 [PubMed 8224848]). One of these factors is TFIIA, which when purified from HeLa extracts consists of 35-, 19-, and 12-kD subunits.[supplied by OMIM, Jul 2010]'