GTF2A1 (NM_201595) Human Untagged Clone

CAT#: SC308068

GTF2A1 (untagged)-Human general transcription factor IIA, 1, 19/37kDa (GTF2A1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GTF2A1
• Synonyms: TF2A1; TFIIA; TFIIA-42; TFIIAL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_201595, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAACTAAAAACTTTATGGGAAAACAAACTAATGCAGTCCAGGGCAGTAGATGGATTTCATTCAGAAG
  AGCAGCAGCTTCTACTGCAAGTTCAACAGCAGCATCAACCCCAGCAGCAGCAGCATCACCACCATCACCA
  TCATCAGCAAGCTCAGCCTCAGCAGACAGTACCTCAGCAAGCGCAGACCCAGCAGGTTCTTATTCCTGCA
  TCACAGCAAGCCACAGCACCACAAGTTATTGTTCCAGATTCTAAGTTGATACAGCATATGAATGCATCAA
  ACATGAGTGCTGCTGCTACAGCTGCTACCTTAGCACTCCCTGCAGGTGTGACTCCTGTTCAGCAGATATT
  AACAAATTCAGGCCAGCTTCTTCAGGTGGTCAGAGCAGCCAATGGTGCCCAATATATCTTTCAGCCTCAG
  CAGTCAGTGGTTCTACAACAACAGGTTATACCACAAATGCAGCCTGGTGGAGTACAAGCTCCTGTTATAC
  AGCAGGTGCTGGCTCCTCTTCCTGGAGGGATTTCACCACAGACAGGTGTCATCATCCAGCCTCAGCAAAT
  CTTATTTACAGGAAATAAGACTCAAGTTATACCTACGACAGTGGCAGCACCTACACCAGCCCAAGCACAG
  ATAACTGCAACTGGCCAGCAGCAACCGCAGGCCCAGCCTGCTCAAACACAAGCTCCATTGGTCTTACAAG
  TTGATGGAACTGGGGATACATCATCTGAAGAAGATGAAGATGAAGAAGAAGACTATGATGATGATGAGGA
  GGAAGACAAAGAGAAAGATGGAGCTGAAGATGGGCAGGTGGAAGAAGAGCCCCTCAATAGTGAAGATGAT
  GTGAGTGATGAGGAAGGACAGGAACTCTTTGACACAGAAAATGTTGTTGTATGCCAATATGATAAGATAC
  ACAGAAGTAAAAACAAATGGAAATTTCATCTCAAGGATGGCATTATGAATCTTAATGGAAGAGATTATAT
  ATTTTCCAAAGCCATTGGAGATGCAGAATGGTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_201595
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_201595.2, NP_963889.1
• RefSeq Size: 5956 bp
• RefSeq ORF: 1014 bp
• Locus ID: 2957
• Cytogenetics: 14q31.1
• Protein Families: Transcription Factors
• Protein Pathways: Basal transcription factors
• Gene Summary: 'Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and several general initiation factors (summarized by DeJong and Roeder, 1993 [PubMed 8224848]). One of these factors is TFIIA, which when purified from HeLa extracts consists of 35-, 19-, and 12-kD subunits.[supplied by OMIM, Jul 2010]'
  Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream, in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.