PDE3A (NM_000921) Human Tagged ORF Clone

CAT#: RC222006

PDE3A (Myc-DDK-tagged)-Human phosphodiesterase 3A, cGMP-inhibited (PDE3A)

Product Images

Western blot validation of overexpression lysate (Cat# LY424434) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222006 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PDE3A
• Synonyms: CGI-PDE; CGI-PDE-A; CGI-PDE A; HTNB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222006 representing NM_000921
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGTGCCCGGCGACGCTGCACGAGTCAGGGACAAGCCCGTCCACAGTGGGGTGAGTCAAGCCCCCA
  CGGCGGGCCGGGACTGCCACCATCGTGCGGACCCCGCATCGCCGCGGGACTCGGGCTGCCGTGGCTGCTG
  GGGAGACCTGGTGCTGCAGCCGCTCCGGAGCTCTCGGAAACTTTCCTCCGCGCTGTGCGCGGGCTCCCTG
  TCCTTTCTGCTGGCGCTGCTGGTGAGGCTGGTCCGCGGGGAGGTCGGCTGTGACCTGGAGCAGTGTAAGG
  AGGCGGCGGCGGCGGAGGAGGAGGAAGCAGCCCCGGGAGCAGAAGGGGGCGTCTTCCCGGGGCCTCGGGG
  AGGTGCTCCCGGGGGCGGTGCGCGGCTCAGCCCCTGGCTGCAGCCCTCGGCGCTGCTCTTCAGTCTCCTG
  TGTGCCTTCTTCTGGATGGGCTTGTACCTCCTGCGCGCCGGGGTGCGCCTGCCTCTGGCTGTCGCGCTGC
  TGGCCGCCTGCTGCGGGGGGGAAGCGCTCGTCCAGATTGGGCTGGGCGTCGGGGAGGATCACTTACTCTC
  ACTCCCCGCCGCGGGGGTGGTGCTCAGCTGCTTGGCCGCCGCGACATGGCTGGTGCTGAGGCTGAGGCTG
  GGCGTCCTCATGATCGCCTTGACTAGCGCGGTCAGGACCGTGTCCCTCATTTCCTTAGAGAGGTTCAAGG
  TCGCCTGGAGACCTTACCTGGCGTACCTGGCCGGCGTGCTGGGGATCCTCTTGGCCAGGTACGTGGAACA
  AATCTTGCCGCAGTCCGCGGAGGCGGCTCCAAGGGAGCATTTGGGGTCCCAGCTGATTGCTGGGACCAAG
  GAAGATATCCCGGTGTTTAAGAGGAGGAGGCGGTCCAGCTCCGTCGTGTCCGCCGAGATGTCCGGCTGCA
  GCAGCAAGTCCCATCGGAGGACCTCCCTGCCCTGTATACCGAGGGAACAGCTCATGGGGCATTCAGAATG
  GGACCACAAACGAGGGCCAAGAGGATCACAGTCTTCAGGAACCAGTATTACTGTGGACATCGCCGTCATG
  GGCGAGGCCCACGGCCTCATTACCGACCTCCTGGCAGACCCTTCTCTTCCACCAAACGTGTGCACATCCT
  TGAGAGCCGTGAGCAACTTGCTCAGCACACAGCTCACCTTCCAGGCCATTCACAAGCCCAGAGTGAATCC
  CGTTACTTCGCTCAGTGAAAACTATACCTGTTCTGACTCTGAAGAGAGCTCTGAAAAAGACAAGCTTGCT
  ATTCCAAAGCGCCTGAGAAGGAGTTTGCCTCCTGGCTTGTTGAGACGAGTTTCTTCCACTTGGACCACCA
  CCACCTCGGCCACAGGTCTACCCACCTTGGAGCCTGCACCAGTACGGAGAGACCGCAGCACCAGCATCAA
  ACTGCAGGAAGCACCTTCATCCAGTCCTGATTCTTGGAATAATCCAGTGATGATGACCCTCACCAAAAGC
  AGATCCTTTACTTCATCCTATGCTATTTCTGCAGCTAACCATGTAAAGGCTAAAAAGCAAAGTCGACCAG
  GTGCCCTCGCTAAAATTTCACCTCTTTCATCGCCCTGCTCCTCACCTCTCCAAGGGACTCCTGCCAGCAG
  CCTGGTCAGCAAAATTTCTGCAGTGCAGTTTCCAGAATCTGCTGACACAACTGCCAAACAAAGCCTAGGT
  TCTCACAGGGCCTTAACTTACACTCAGAGTGCCCCAGACCTATCCCCTCAAATCCTGACTCCACCTGTTA
  TATGTAGCAGCTGTGGCAGACCATATTCCCAAGGGAATCCTGCTGATGAGCCCCTGGAGAGAAGTGGGGT
  AGCCACTCGGACACCAAGTAGAACAGATGACACTGCTCAAGTTACCTCTGATTATGAAACCAATAACAAC
  AGTGACAGCAGTGACATTGTACAGAATGAAGATGAAACAGAGTGCCTGAGAGAGCCTCTGAGGAAAGCAT
  CGGCTTGCAGCACCTATGCTCCTGAGACCATGATGTTTCTGGACAAACCAATTCTTGCTCCCGAACCTCT
  TGTCATGGATAACCTGGACTCAATTATGGAGCAGCTAAATACTTGGAATTTTCCAATTTTTGATTTAGTG
  GAAAATATAGGAAGAAAATGTGGCCGTATTCTTAGTCAGGTATCTTACAGACTTTTTGAAGACATGGGCC
  TGTTTGAAGCTTTTAAAATTCCAATTAGGGAATTTATGAATTATTTTCATGCTTTGGAGATTGGATATAG
  GGATATTCCTTATCATAACAGAATCCATGCCACTGATGTTTTACATGCTGTTTGGTATCTTACTACACAG
  CCTATTCCAGGCCTCTCAACTGTGATTAATGATCATGGTTCAACCAGTGATTCAGATTCTGACAGTGGAT
  TTACACATGGACATATGGGATATGTATTCTCAAAAACGTATAATGTGACAGATGATAAATACGGATGTCT
  GTCTGGGAATATCCCTGCCTTGGAGTTGATGGCGCTGTATGTGGCTGCAGCCATGCACGATTATGATCAT
  CCAGGAAGGACTAATGCTTTCCTGGTTGCAACTAGTGCTCCTCAGGCGGTGCTATATAACGATCGTTCAG
  TTTTGGAGAATCATCACGCAGCTGCTGCATGGAATCTTTTCATGTCCCGGCCAGAGTATAACTTCTTAAT
  TAACCTTGACCATGTGGAATTTAAGCATTTCCGTTTCCTTGTCATTGAAGCAATTTTGGCCACTGACCTG
  AAGAAACACTTTGACTTCGTAGCCAAATTTAATGGCAAGGTAAATGATGATGTTGGAATAGATTGGACCA
  ATGAAAATGATCGTCTACTGGTTTGTCAAATGTGTATAAAGTTGGCTGATATCAATGGTCCAGCTAAATG
  TAAAGAACTCCATCTTCAGTGGACAGATGGTATTGTCAATGAATTTTATGAACAGGGTGATGAAGAGGCC
  AGCCTTGGATTACCCATAAGCCCCTTCATGGATCGTTCTGCTCCTCAGCTGGCCAACCTTCAGGAATCCT
  TCATCTCTCACATTGTGGGGCCTCTGTGCAACTCCTATGATTCAGCAGGACTAATGCCTGGAAAATGGGT
  GGAAGACAGCGATGAGTCAGGAGATACTGATGACCCAGAAGAAGAGGAGGAAGAAGCACCAGCACCAAAT
  GAAGAGGAAACCTGTGAAAATAATGAATCTCCAAAAAAGAAGACTTTCAAAAGGAGAAAAATCTACTGCC
  AAATAACTCAGCACCTCTTACAGAACCACAAGATGTGGAAGAAAGTCATTGAAGAGGAGCAACGGTTGGC
  AGGCATAGAAAATCAATCCCTGGACCAGACCCCTCAGTCGCACTCTTCAGAACAGATCCAGGCTATCAAG
  GAAGAAGAAGAAGAGAAAGGGAAACCAAGAGGCGAGGAGATACCAACCCAAAAGCCAGACCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222006 representing NM_000921
  Red=Cloning site Green=Tags(s)
  
  MAVPGDAARVRDKPVHSGVSQAPTAGRDCHHRADPASPRDSGCRGCWGDLVLQPLRSSRKLSSALCAGSL
  SFLLALLVRLVRGEVGCDLEQCKEAAAAEEEEAAPGAEGGVFPGPRGGAPGGGARLSPWLQPSALLFSLL
  CAFFWMGLYLLRAGVRLPLAVALLAACCGGEALVQIGLGVGEDHLLSLPAAGVVLSCLAAATWLVLRLRL
  GVLMIALTSAVRTVSLISLERFKVAWRPYLAYLAGVLGILLARYVEQILPQSAEAAPREHLGSQLIAGTK
  EDIPVFKRRRRSSSVVSAEMSGCSSKSHRRTSLPCIPREQLMGHSEWDHKRGPRGSQSSGTSITVDIAVM
  GEAHGLITDLLADPSLPPNVCTSLRAVSNLLSTQLTFQAIHKPRVNPVTSLSENYTCSDSEESSEKDKLA
  IPKRLRRSLPPGLLRRVSSTWTTTTSATGLPTLEPAPVRRDRSTSIKLQEAPSSSPDSWNNPVMMTLTKS
  RSFTSSYAISAANHVKAKKQSRPGALAKISPLSSPCSSPLQGTPASSLVSKISAVQFPESADTTAKQSLG
  SHRALTYTQSAPDLSPQILTPPVICSSCGRPYSQGNPADEPLERSGVATRTPSRTDDTAQVTSDYETNNN
  SDSSDIVQNEDETECLREPLRKASACSTYAPETMMFLDKPILAPEPLVMDNLDSIMEQLNTWNFPIFDLV
  ENIGRKCGRILSQVSYRLFEDMGLFEAFKIPIREFMNYFHALEIGYRDIPYHNRIHATDVLHAVWYLTTQ
  PIPGLSTVINDHGSTSDSDSDSGFTHGHMGYVFSKTYNVTDDKYGCLSGNIPALELMALYVAAAMHDYDH
  PGRTNAFLVATSAPQAVLYNDRSVLENHHAAAAWNLFMSRPEYNFLINLDHVEFKHFRFLVIEAILATDL
  KKHFDFVAKFNGKVNDDVGIDWTNENDRLLVCQMCIKLADINGPAKCKELHLQWTDGIVNEFYEQGDEEA
  SLGLPISPFMDRSAPQLANLQESFISHIVGPLCNSYDSAGLMPGKWVEDSDESGDTDDPEEEEEEAPAPN
  EEETCENNESPKKKTFKRRKIYCQITQHLLQNHKMWKKVIEEEQRLAGIENQSLDQTPQSHSSEQIQAIK
  EEEEEKGKPRGEEIPTQKPDQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000921
• ORF Size: 3423 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000921.1, NM_000921.2, NM_000921.3, NM_000921.4, NP_000912.3
• RefSeq Size: 4124 bp
• RefSeq ORF: 3426 bp
• Locus ID: 5139
• Cytogenetics: 12p12.2
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Insulin signaling pathway, Progesterone-mediated oocyte maturation, Purine metabolism
• MW: 124.8 kDa
• Gene Summary: 'This gene encodes a member of the cGMP-inhibited cyclic nucleotide phosphodiesterase (cGI-PDE) family. cGI-PDE enzymes hydrolyze both cAMP and cGMP, and play critical roles in many cellular processes by regulating the amplitude and duration of intracellular cyclic nucleotide signals. The encoded protein mediates platelet aggregation and also plays important roles in cardiovascular function by regulating vascular smooth muscle contraction and relaxation. Inhibitors of the encoded protein may be effective in treating congestive heart failure. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]'