MTUS1 (NM_001001925) Human Tagged ORF Clone

CAT#: RC222093

MTUS1 (Myc-DDK-tagged)-Human microtubule associated tumor suppressor 1 (MTUS1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MTUS1
• Synonyms: ATBP; ATIP; ATIP3; ICIS; MP44; MTSG1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222093 representing NM_001001925
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGATGATAATTCAGATGATAAAATAGAAGATGAATTGCAAACCTTCTTTACCAGTGATAAAGATG
  GAAATACACATGCATACAACCCGAAATCACCACCTACACAAAACTCTTCAGCCAGCAGTGTGAACTGGAA
  TTCTGCCAACCCAGATGACATGGTGGTTGATTATGAAACTGACCCTGCTGTAGTTACTGGTGAAAATATT
  TCTTTAAGCCTTCAGGGTGTTGAAGTATTTGGTCATGAAAAGTCTTCTAGTGATTTCATTAGTAAGCAGG
  TGTTAGATATGCATAAAGATTCTATTTGTCAGTGTCCTGCACTTGTAGGTACTGAGAAGCCCAAATATCT
  GCAACACAGTTGTCATTCCCTAGAAGCAGTTGAGGGCCAGAGTGTTGAGCCATCTTTGCCTTTTGTGTGG
  AAGCCTAATGACAATTTGAACTGTGCAGGCTACTGTGATGCCTTGGAGCTAAACCAAACATTTGACATGA
  CAGTGGATAAAGTTAACTGCACCTTTATATCACATCATGCCATCGGAAAGAGTCAGTCCTTCCATACTGC
  TGGAAGCCTGCCACCAACTGGTAGGAGAAGTGGAAGTACATCTTCTTTATCCTATTCCACTTGGACATCT
  TCCCATTCTGATAAGACGCATGCAAGAGAAACTACTTATGATAGAGAAAGCTTTGAAAACCCTCAAGTCA
  CACCATCAGAAGCCCAAGACATGACTTACACAGCATTTTCTGATGTGGTGATGCAAAGTGAGGTTTTTGT
  TTCAGATATTGGAAATCAGTGTGCATGTTCTTCAGGAAAGGTCACCAGTGAGTACACAGATGGATCACAA
  CAAAGACTAGTTGGAGAAAAGGAGACACAAGCACTAACACCAGTTTCTGATGGCATGGAAGTCCCCAATG
  ATTCTGCATTACAAGAGTTCTTTTGTTTATCCCATGATGAATCCAATAGCGAACCACATTCACAGAGCTC
  ATACAGGCACAAGGAAATGGGCCAAAATCTGAGAGAGACAGTGTCCTATTGTCTTATTGATGATGAATGC
  CCTTTAATGGTGCCAGCTTTTGATAAGAGCGAAGCTCAAGTGCTGAACCCAGAGCATAAAGTCACTGAGA
  CTGAAGACACACAAATGGTCTCCAAAGGAAAGGATTTGGGAACCCAAAATCATACCTCAGAATTGATTCT
  AAGTAGCCCGCCAGGACAAAAGGTGGGCTCGTCATTTGGACTGACTTGGGATGCAAATGATATGGTCATT
  AGCACAGACAAAACGATGTGCATGTCAACACCAGTCCTAGAACCCACAAAAGTAACCTTTTCTGTTTCAC
  CGATTGAAGCGACGGAGAAATGTAAGAAAGTGGAGAAGGGTAATCGAGGGCTTAAAAACATACCAGACTC
  GAAGGAGGCACCTGTGAACCTGTGTAAACCCAGTTTAGGAAAATCAACAATCAAAACGAATACCCCAATA
  GGCTGCAAAGTTAGAAAAACTGAAATTATAAGTTACCCAAGACCAAACTTCAAGAATGTCAAAGCAAAAG
  TTATGTCTAGAGCAGTGTTGCAGCCCAAAGATGCTGCTTTATCAAAGGTCACGCCCAGACCTCAGCAGAC
  CAGTGCCTCATCACCCTCATCAGTGAATTCAAGACAACAAACAGTCTTGAGCAGAACACCGAGATCTGAC
  TTGAATGCAGACAAAAAAGCAGAAATTCTAATTAACAAGACACATAAGCAGCAGTTTAATAAACTCATTA
  CTAGCCAGGCTGTGCATGTTACAACTCATTCTAAAAATGCTTCACACAGGGTTCCAAGAACAACATCTGC
  CGTGAAATCGAATCAGGAAGATGTTGACAAAGCCAGTTCTTCTAACTCAGCATGCGAGACCGGGTCCGTT
  TCTGCGTTGTTTCAGAAGATCAAAGGCATACTCCCTGTTAAAATGGAAAGTGCAGAATGTTTGGAAATGA
  CCTATGTTCCCAACATTGATAGGATTAGCCCTGAAAAGAAGGGTGAAAAAGAAAATGGGACATCTATGGA
  AAAACAAGAGCTGAAACAAGAGATTATGAATGAGACTTTTGAATATGGTTCTCTGTTTTTGGGCTCTGCT
  TCAAAAACAACGACCACCTCAGGTAGGAATATATCCAAGCCTGACTCCTGCGGTTTGAGGCAAATAGCTG
  CTCCAAAAGCCAAAGTGGGGCCCCCTGTTTCCTGTTTGAGGCGGAACAGTGACAATAGAAATCCCAGTGC
  TGATCGAGCCGTATCTCCTCAGAGGATCAGGCGTGTGTCCAGTTCTGCTGGTAATGCCGCTGTCATCAAA
  TATGAGGAGAAACCTCCAAAACCAGCATTTCAGAATGGTTCCTCAGGATCCTTTTATTTGAAGCCTTTGG
  TATCCAGGGCTCATGTTCACTTGATGAAAACTCCTCCAAAAGGTCCTTCGAGAAAAAATTTATTTACAGC
  TCTTAATGCAGTTGAAAAGAGCAGGCAAAAGAATCCTCGAAGCTTATGTATCCAGCCACAGACAGCTCCC
  GATGCGCTGCCCCCTGAGAAAACACTTGAATTGACGCAATATAAAACAAAATGTGAAAACCAAAGTGGAT
  TTATCCTGCAGCTCAAGCAGCTTCTTGCCTGTGGTAATACCAAGTTTGAGGCATTGACAGTTGTGATTCA
  GCACCTGCTGTCTGAGCGGGAGGAAGCACTGAAACAACACAAAACCCTATCTCAAGAACTTGTTAACCTC
  CGGGGAGAGCTAGTCACTGCTTCAACCACCTGTGAGAAATTAGAAAAAGCCAGGAATGAGTTACAAACAG
  TGTATGAAGCATTCGTCCAGCAGCACCAGGCTGAAAAAACAGAACGAGAGAATCGGCTTAAAGAGTTTTA
  CACCAGGGAGTATGAAAAGCTTCGGGACACTTACATTGAAGAAGCAGAGAAGTACAAAATGCAATTGCAA
  GAGCAGTTTGACAACTTAAATGCTGCGCATGAAACCTCTAAGTTGGAAATTGAAGCTAGCCACTCAGAGA
  AACTTGAATTGCTAAAGAAGGCCTATGAAGCCTCCCTTTCAGAAATTAAGAAAGGCCATGAAATAGAAAA
  GAAATCGCTTGAAGATTTACTTTCTGAGAAGCAGGAATCGCTAGAGAAGCAAATCAATGATCTGAAGAGT
  GAAAATGATGCTTTAAATGAAAAATTGAAATCAGAAGAACAAAAAAGAAGAGCAAGAGAAAAAGCAAATT
  TGAAAAATCCTCAGATCATGTATCTAGAACAGGAGTTAGAAAGCCTGAAAGCTGTGTTAGAGATCAAGAA
  TGAGAAACTGCATCAACAGGACATCAAGTTAATGAAAATGGAGAAACTGGTGGACAACAACACAGCATTG
  GTTGACAAATTGAAGCGTTTCCAGCAGGAGAATGAAGAATTGAAAGCTCGGATGGACAAGCACATGGCAA
  TCTCAAGGCAGCTTTCCACGGAGCAGGCTGTTCTGCAAGAGTCGCTGGAGAAGGAGTCGAAAGTCAACAA
  GCGACTCTCTATGGAAAACGAGGAGCTTCTGTGGAAACTGCACAATGGGGACCTGTGTAGCCCCAAGAGA
  TCCCCCACATCCTCCGCCATCCCTTTGCAGTCACCAAGGAATTCGGGCTCCTTCCCTAGCCCCAGCATTT
  CACCCAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222093 representing NM_001001925
  Red=Cloning site Green=Tags(s)
  
  MTDDNSDDKIEDELQTFFTSDKDGNTHAYNPKSPPTQNSSASSVNWNSANPDDMVVDYETDPAVVTGENI
  SLSLQGVEVFGHEKSSSDFISKQVLDMHKDSICQCPALVGTEKPKYLQHSCHSLEAVEGQSVEPSLPFVW
  KPNDNLNCAGYCDALELNQTFDMTVDKVNCTFISHHAIGKSQSFHTAGSLPPTGRRSGSTSSLSYSTWTS
  SHSDKTHARETTYDRESFENPQVTPSEAQDMTYTAFSDVVMQSEVFVSDIGNQCACSSGKVTSEYTDGSQ
  QRLVGEKETQALTPVSDGMEVPNDSALQEFFCLSHDESNSEPHSQSSYRHKEMGQNLRETVSYCLIDDEC
  PLMVPAFDKSEAQVLNPEHKVTETEDTQMVSKGKDLGTQNHTSELILSSPPGQKVGSSFGLTWDANDMVI
  STDKTMCMSTPVLEPTKVTFSVSPIEATEKCKKVEKGNRGLKNIPDSKEAPVNLCKPSLGKSTIKTNTPI
  GCKVRKTEIISYPRPNFKNVKAKVMSRAVLQPKDAALSKVTPRPQQTSASSPSSVNSRQQTVLSRTPRSD
  LNADKKAEILINKTHKQQFNKLITSQAVHVTTHSKNASHRVPRTTSAVKSNQEDVDKASSSNSACETGSV
  SALFQKIKGILPVKMESAECLEMTYVPNIDRISPEKKGEKENGTSMEKQELKQEIMNETFEYGSLFLGSA
  SKTTTTSGRNISKPDSCGLRQIAAPKAKVGPPVSCLRRNSDNRNPSADRAVSPQRIRRVSSSAGNAAVIK
  YEEKPPKPAFQNGSSGSFYLKPLVSRAHVHLMKTPPKGPSRKNLFTALNAVEKSRQKNPRSLCIQPQTAP
  DALPPEKTLELTQYKTKCENQSGFILQLKQLLACGNTKFEALTVVIQHLLSEREEALKQHKTLSQELVNL
  RGELVTASTTCEKLEKARNELQTVYEAFVQQHQAEKTERENRLKEFYTREYEKLRDTYIEEAEKYKMQLQ
  EQFDNLNAAHETSKLEIEASHSEKLELLKKAYEASLSEIKKGHEIEKKSLEDLLSEKQESLEKQINDLKS
  ENDALNEKLKSEEQKRRAREKANLKNPQIMYLEQELESLKAVLEIKNEKLHQQDIKLMKMEKLVDNNTAL
  VDKLKRFQQENEELKARMDKHMAISRQLSTEQAVLQESLEKESKVNKRLSMENEELLWKLHNGDLCSPKR
  SPTSSAIPLQSPRNSGSFPSPSISPR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001001925
• ORF Size: 3648 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001001925.1, NM_001001925.2, NP_001001925.1
• RefSeq Size: 6273 bp
• RefSeq ORF: 3651 bp
• Locus ID: 57509
• Cytogenetics: 8p22
• Protein Families: Druggable Genome, Transcription Factors, Transmembrane
• MW: 135.7 kDa
• Gene Summary: This gene encodes a protein which contains a C-terminal domain able to interact with the angiotension II (AT2) receptor and a large coiled-coil region allowing dimerization. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. One of the transcript variants has been shown to encode a mitochondrial protein that acts as a tumor suppressor and partcipates in AT2 signaling pathways. Other variants may encode nuclear or transmembrane proteins but it has not been determined whether they also participate in AT2 signaling pathways. [provided by RefSeq, Jul 2008]