MTUS1 (NM_001001931) Human Tagged ORF Clone

CAT#: RC222152

MTUS1 (Myc-DDK-tagged)-Human microtubule associated tumor suppressor 1 (MTUS1), transcript variant 4

Product Images

Western blot validation of overexpression lysate (Cat# LY424280) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222152 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MTUS1
• Synonyms: ATBP; ATIP; ATIP3; ICIS; MP44; MTSG1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222152 representing NM_001001931
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGTACCAGGAGGATTTCGCAGTTGTACAGAAACTGATATTTCTTCAAAAATCTTTATCAATTCTA
  CACTCACCCCACCGGCTGGTTCAGAGAGGCACTATGATGCTACCTTATTGACACTGCTGGTCGTGGGATC
  GTACAGCCTTTGTATAATTCCTTTGTTAGCCACGTTTACTGGGAAAAAAACTGGTAATGCCGCTGTCATC
  AAATATGAGGAGAAACCTCCAAAACCAGCATTTCAGAATGGTTCCTCAGGATCCTTTTATTTGAAGCCTT
  TGGTATCCAGGGCTCATGTTCACTTGATGAAAACTCCTCCAAAAGGTCCTTCGAGAAAAAATTTATTTAC
  AGCTCTTAATGCAGTTGAAAAGAGCAGGCAAAAGAATCCTCGAAGCTTATGTATCCAGCCACAGACAGCT
  CCCGATGCGCTGCCCCCTGAGAAAACACTTGAATTGACGCAATATAAAACAAAATGTGAAAACCAAAGTG
  GATTTATCCTGCAGCTCAAGCAGCTTCTTGCCTGTGGTAATACCAAGTTTGAGGCATTGACAGTTGTGAT
  TCAGCACCTGCTGTCTGAGCGGGAGGAAGCACTGAAACAACACAAAACCCTATCTCAAGAACTTGTTAAC
  CTCCGGGGAGAGCTAGTCACTGCTTCAACCACCTGTGAGAAATTAGAAAAAGCCAGGAATGAGTTACAAA
  CAGTGTATGAAGCATTCGTCCAGCAGCACCAGGCTGAAAAAACAGAACGAGAGAATCGGCTTAAAGAGTT
  TTACACCAGGGAGTATGAAAAGCTTCGGGACACTTACATTGAAGAAGCAGAGAAGTACAAAATGCAATTG
  CAAGAGCAGTTTGACAACTTAAATGCTGCGCATGAAACCTCTAAGTTGGAAATTGAAGCTAGCCACTCAG
  AGAAACTTGAATTGCTAAAGAAGGCCTATGAAGCCTCCCTTTCAGAAATTAAGAAAGGCCATGAAATAGA
  AAAGAAATCGCTTGAAGATTTACTTTCTGAGAAGCAGGAATCGCTAGAGAAGCAAATCAATGATCTGAAG
  AGTGAAAATGATGCTTTAAATGAAAAATTGAAATCAGAAGAACAAAAAAGAAGAGCAAGAGAAAAAGCAA
  ATTTGAAAAATCCTCAGATCATGTATCTAGAACAGGAGTTAGAAAGCCTGAAAGCTGTGTTAGAGATCAA
  GAATGAGAAACTGCATCAACAGGACATCAAGTTAATGAAAATGGAGAAACTGGTGGACAACAACACAGCA
  TTGGTTGACAAATTGAAGCGTTTCCAGCAGGAGAATGAAGAATTGAAAGCTCGGATGGACAAGCACATGG
  CAATCTCAAGGCAGCTTTCCACGGAGCAGGCTGTTCTGCAAGAGTCGCTGGAGAAGGAGTCGAAAGTCAA
  CAAGCGACTCTCTATGGAAAACGAGGAGCTTCTGTGGAAACTGCACAATGGGGACCTGTGTAGCCCCAAG
  AGATCCCCCACATCCTCCGCCATCCCTTTGCAGTCACCAAGGAATTCGGGCTCCTTCCCTAGCCCCAGCA
  TTTCACCCAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222152 representing NM_001001931
  Red=Cloning site Green=Tags(s)
  
  MTVPGGFRSCTETDISSKIFINSTLTPPAGSERHYDATLLTLLVVGSYSLCIIPLLATFTGKKTGNAAVI
  KYEEKPPKPAFQNGSSGSFYLKPLVSRAHVHLMKTPPKGPSRKNLFTALNAVEKSRQKNPRSLCIQPQTA
  PDALPPEKTLELTQYKTKCENQSGFILQLKQLLACGNTKFEALTVVIQHLLSEREEALKQHKTLSQELVN
  LRGELVTASTTCEKLEKARNELQTVYEAFVQQHQAEKTERENRLKEFYTREYEKLRDTYIEEAEKYKMQL
  QEQFDNLNAAHETSKLEIEASHSEKLELLKKAYEASLSEIKKGHEIEKKSLEDLLSEKQESLEKQINDLK
  SENDALNEKLKSEEQKRRAREKANLKNPQIMYLEQELESLKAVLEIKNEKLHQQDIKLMKMEKLVDNNTA
  LVDKLKRFQQENEELKARMDKHMAISRQLSTEQAVLQESLEKESKVNKRLSMENEELLWKLHNGDLCSPK
  RSPTSSAIPLQSPRNSGSFPSPSISPR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001001931
• ORF Size: 1551 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001001931.1, NM_001001931.2, NP_001001931.1
• RefSeq Size: 4018 bp
• RefSeq ORF: 1554 bp
• Locus ID: 57509
• Cytogenetics: 8p22
• Protein Families: Druggable Genome, Transcription Factors, Transmembrane
• MW: 58.8 kDa
• Gene Summary: This gene encodes a protein which contains a C-terminal domain able to interact with the angiotension II (AT2) receptor and a large coiled-coil region allowing dimerization. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. One of the transcript variants has been shown to encode a mitochondrial protein that acts as a tumor suppressor and partcipates in AT2 signaling pathways. Other variants may encode nuclear or transmembrane proteins but it has not been determined whether they also participate in AT2 signaling pathways. [provided by RefSeq, Jul 2008]