PDE11A (NM_001077197) Human Tagged ORF Clone

CAT#: RC222400

PDE11A (Myc-DDK-tagged)-Human phosphodiesterase 11A (PDE11A), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PDE11A
• Synonyms: PPNAD2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222400 representing NM_001077197
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAAGCAGGCAAGAAGACCTTTATTCAGAAATGTGCTCAGTGCCACACAGTGGAAAAAGGTGAAAA
  TCACAAGACTGGTCCAAATCTCTGGGGCCTCTTTGGCTGAAAAACAGGAAAAGCACCAGGATTTTCTTAT
  ACAGAGGCAAACAAAAACAAAGGATCGACGATTCAATGATGAAATCGACAAGCTAACTGGATACAAGACA
  AAATCATTATTGTGCATGCCTATCCGAAGCAGTGATGGTGAGATTATTGGTGTGGCCCAAGCGATAAATA
  AGATTCCTGAAGGAGCTCCATTTACTGAAGATGATGAAAAAGTTATGCAGATGTATCTTCCATTTTGTGG
  AATCGCCATATCTAACGCTCAGCTCTTTGCTGCCTCAAGGAAAGAATATGAAAGAAGCAGAGCTTTGCTA
  GAGGTGGTTAATGACCTCTTTGAAGAACAGACTGACCTGGAGAAAATTGTCAAGAAAATAATGCATCGGG
  CCCAAACTCTGCTGAAATGTGAACGCTGTTCTGTTTTACTCCTAGAGGACATCGAATCACCAGTGGTGAA
  ATTTACCAAATCCTTTGAATTGATGTCCCCAAAGTGCAGTGCTGATGCTGAGAACAGTTTCAAAGAAAGC
  ATGGAGAAATCATCATACTCCGACTGGCTAATAAATAACAGCATTGCTGAGCTGGTTGCTTCAACAGGCC
  TTCCAGTGAACATCAGTGATGCCTACCAGGATCCGCGCTTTGATGCAGAGGCAGACCAGATATCTGGTTT
  TCACATAAGATCTGTTCTTTGTGTCCCTATTTGGAATAGCAACCACCAAATAATTGGAGTGGCTCAAGTG
  TTAAACAGACTTGATGGGAAACCTTTTGATGATGCAGATCAACGACTTTTTGAGGCTTTTGTCATCTTTT
  GTGGACTTGGCATCAACAACACAATTATGTATGATCAAGTGAAGAAGTCCTGGGCCAAGCAGTCTGTGGC
  TCTTGATGTGCTATCATACCATGCAACATGTTCAAAAGCTGAAGTTGACAAGTTTAAGGCAGCCAACATC
  CCTCTGGTGTCAGAACTTGCCATCGATGACATTCATTTTGATGACTTTTCTCTCGACGTTGATGCCATGA
  TCACAGCTGCTCTCCGGATGTTCATGGAGCTGGGGATGGTACAGAAATTTAAAATTGACTATGAGACACT
  GTGTAGGTGGCTTTTGACAGTGAGGAAAAACTATCGGATGGTTCTATACCACAACTGGAGACATGCCTTC
  AACGTGTGTCAGCTGATGTTCGCGATGTTAACCACTGCTGGGTTTCAAGACATTCTGACCGAGGTGGAAA
  TTTTAGCGGTGATTGTGGGATGCCTGTGTCATGACCTCGACCACAGGGGAACCAACAATGCCTTCCAAGC
  TAAGAGTGGCTCTGCCCTGGCCCAACTCTATGGAACCTCTGCTACCTTGGAGCATCACCATTTCAACCAC
  GCCGTGATGATCCTTCAAAGTGAGGGTCACAATATCTTTGCTAACCTGTCCTCCAAGGAATATAGTGACC
  TTATGCAGCTTTTGAAGCAGTCAATATTGGCAACAGACCTCACGCTGTACTTTGAGAGGAGAACTGAATT
  CTTTGAACTTGTCAGTAAAGGAGAATACGATTGGAACATCAAAAACCATCGTGATATATTTCGATCAATG
  TTAATGACAGCCTGTGACCTTGGAGCCGTGACCAAACCGTGGGAGATCTCCAGACAGGTGGCAGAACTTG
  TAACCAGTGAGTTCTTCGAACAAGGAGATCGGGAGAGATTAGAGCTCAAACTCACTCCTTCAGCAATTTT
  TGATCGGAACCGGAAGGATGAACTGCCTCGGTTGCAACTGGAGTGGATTGATAGCATCTGCATGCCTTTG
  TATCAGGCACTGGTGAAGGTCAACGTGAAACTGAAGCCGATGCTAGATTCAGTAGCTACAAACAGAAGTA
  AGTGGGAAGAGCTACACCAAAAACGACTGCTGGCCTCAACTGCCTCATCCTCCCCTGCCAGTGTTATGGT
  AGCCAAGGAAGACAGGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222400 representing NM_001077197
  Red=Cloning site Green=Tags(s)
  
  MLKQARRPLFRNVLSATQWKKVKITRLVQISGASLAEKQEKHQDFLIQRQTKTKDRRFNDEIDKLTGYKT
  KSLLCMPIRSSDGEIIGVAQAINKIPEGAPFTEDDEKVMQMYLPFCGIAISNAQLFAASRKEYERSRALL
  EVVNDLFEEQTDLEKIVKKIMHRAQTLLKCERCSVLLLEDIESPVVKFTKSFELMSPKCSADAENSFKES
  MEKSSYSDWLINNSIAELVASTGLPVNISDAYQDPRFDAEADQISGFHIRSVLCVPIWNSNHQIIGVAQV
  LNRLDGKPFDDADQRLFEAFVIFCGLGINNTIMYDQVKKSWAKQSVALDVLSYHATCSKAEVDKFKAANI
  PLVSELAIDDIHFDDFSLDVDAMITAALRMFMELGMVQKFKIDYETLCRWLLTVRKNYRMVLYHNWRHAF
  NVCQLMFAMLTTAGFQDILTEVEILAVIVGCLCHDLDHRGTNNAFQAKSGSALAQLYGTSATLEHHHFNH
  AVMILQSEGHNIFANLSSKEYSDLMQLLKQSILATDLTLYFERRTEFFELVSKGEYDWNIKNHRDIFRSM
  LMTACDLGAVTKPWEISRQVAELVTSEFFEQGDRERLELKLTPSAIFDRNRKDELPRLQLEWIDSICMPL
  YQALVKVNVKLKPMLDSVATNRSKWEELHQKRLLASTASSSPASVMVAKEDRN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001077197
• ORF Size: 2049 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001077197.1, NP_001070665.1
• RefSeq Size: 8316
• RefSeq ORF: 2052
• Locus ID: 50940
• Protein Families: Druggable Genome
• Protein Pathways: Progesterone-mediated oocyte maturation, Purine metabolism
• MW: 77.9 kDa
• Gene Summary: The 3',5'-cyclic nucleotides cAMP and cGMP function as second messengers in a wide variety of signal transduction pathways. 3',5'-cyclic nucleotide phosphodiesterases (PDEs) catalyze the hydrolysis of cAMP and cGMP to the corresponding 5'-monophosphates and provide a mechanism to downregulate cAMP and cGMP signaling. This gene encodes a member of the PDE protein superfamily. Mutations in this gene are a cause of Cushing disease and adrenocortical hyperplasia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]