CYP7B1 (NM_004820) Human Tagged ORF Clone

CAT#: RC222647

CYP7B1 (Myc-DDK-tagged)-Human cytochrome P450, family 7, subfamily B, polypeptide 1 (CYP7B1)

Product Images

Coomassie blue staining of purified CYP7B1 protein (Cat# TP322647). The protein was produced from HEK293T cells transfected with CYP7B1 cDNA clone (Cat# RC222647) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CYP7B1
• Synonyms: CBAS3; CP7B; SPG5A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222647 representing NM_004820
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGGAGAAGTGTCCGCGGCCACGGGCCGCTTTTCGCTGGAGCGGTTGGGCCTCCCGGGCCTGGCCC
  TCGCCGCGGCCCTGCTGCTCCTGGCCCTCTGCTTGCTTGTCCGGCGCACCAGGAGACCCGGTGAGCCTCC
  ATTGATAAAAGGTTGGCTTCCTTATCTTGGAGTGGTCCTGAACTTACGAAAAGACCCCTTAAGGTTCATG
  AAAACACTTCAAAAGCAACATGGTGACACTTTCACAGTTCTTCTTGGTGGAAAGTACATAACATTTATCC
  TGGACCCCTTCCAGTACCAGCTAGTGATAAAAAATCATAAACAATTAAGCTTTCGAGTATTTTCTAATAA
  ATTATTAGAGAAAGCATTTAGCATCAGTCAGTTGCAAAAAAATCATGACATGAATGATGAGCTTCACCTC
  TGCTATCAATTTTTGCAAGGCAAATCTTTGGACATACTCTTGGAAAGCATGATGCAGAATCTAAAACAAG
  TTTTTGAACCCCAGCTGTTAAAAACCACAAGTTGGGACACGGCAGAACTGTATCCATTCTGCAGCTCAAT
  AATATTTGAGATCACATTTACAACTATATATGGAAAAGTTATTGTTTGTGACAACAACAAATTTATTAGT
  GAGCTAAGAGATGATTTTTTAAAATTTGATGACAAGTTTGCATATTTAGTATCCAACATACCCATTGAGC
  TTCTAGGAAATGTCAAGTCTATTAGAGAGAAAATTATAAAATGCTTCTCATCAGAAAAGTTAGCCAAGAT
  GCAAGGATGGTCAGAAGTTTTTCAAAGCAGGCAAGATGTCCTGGAGAAATATTATGTGCACGAGGACCTT
  GAAATAGGAGCACATCATTTAGGCTTTCTCTGGGCCTCTGTGGCAAACACTATTCCAACTATGTTCTGGG
  CAATGTATTATCTTCTGCGGCACCCAGAAGCTATGGCAGCAGTGCGTGACGAAATTGACCGTTTGCTGCA
  GTCAACAGGTCAAAAGAAAGGGTCTGGATTTCCCATCCACCTCACCAGAGAACAATTGGACAGCCTAATC
  TGCCTAGAAAGCAGCATTTTTGAAGCTTTACGACTGTCCTCATATTCAACCACCATTCGTTTTGTTGAGG
  AGGATTTGACTCTCAGTTCAGAGACCGGGGACTACTGTGTGCGAAAGGGAGACTTGGTAGCCATCTTTCC
  TCCAGTCCTACATGGTGACCCTGAAATCTTTGAAGCTCCAGAGGAGTTTAGATATGATCGTTTTATAGAA
  GATGGTAAGAAGAAAACCACCTTTTTCAAAAGAGGGAAAAAGCTGAAGTGTTACCTAATGCCGTTTGGAA
  CTGGAACCAGCAAATGTCCAGGCCGATTTTTTGCACTTATGGAAATAAAACAATTGTTGGTTATACTTTT
  AACTTATTTTGATTTAGAAATAATTGATGATAAGCCCATAGGACTAAACTACAGCCGCTTGTTGTTTGGT
  ATTCAGTATCCAGATTCTGATGTTTTATTTAGATACAAAGTGAAATCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222647 representing NM_004820
  Red=Cloning site Green=Tags(s)
  
  MAGEVSAATGRFSLERLGLPGLALAAALLLLALCLLVRRTRRPGEPPLIKGWLPYLGVVLNLRKDPLRFM
  KTLQKQHGDTFTVLLGGKYITFILDPFQYQLVIKNHKQLSFRVFSNKLLEKAFSISQLQKNHDMNDELHL
  CYQFLQGKSLDILLESMMQNLKQVFEPQLLKTTSWDTAELYPFCSSIIFEITFTTIYGKVIVCDNNKFIS
  ELRDDFLKFDDKFAYLVSNIPIELLGNVKSIREKIIKCFSSEKLAKMQGWSEVFQSRQDVLEKYYVHEDL
  EIGAHHLGFLWASVANTIPTMFWAMYYLLRHPEAMAAVRDEIDRLLQSTGQKKGSGFPIHLTREQLDSLI
  CLESSIFEALRLSSYSTTIRFVEEDLTLSSETGDYCVRKGDLVAIFPPVLHGDPEIFEAPEEFRYDRFIE
  DGKKKTTFFKRGKKLKCYLMPFGTGTSKCPGRFFALMEIKQLLVILLTYFDLEIIDDKPIGLNYSRLLFG
  IQYPDSDVLFRYKVKS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004820
• ORF Size: 1518 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004820.1, NM_004820.2, NM_004820.3, NM_004820.4, NP_004811.1
• RefSeq Size: 2395
• RefSeq ORF: 1521
• Locus ID: 9420
• Domains: p450
• Protein Families: Druggable Genome, P450, Transmembrane
• Protein Pathways: Primary bile acid biosynthesis
• MW: 58.3 kDa
• Gene Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway of extrahepatic tissues, which converts cholesterol to bile acids. This enzyme likely plays a minor role in total bile acid synthesis, but may also be involved in the development of atherosclerosis, neurosteroid metabolism and sex hormone synthesis. Mutations in this gene have been associated with hereditary spastic paraplegia (SPG5 or HSP), an autosomal recessive disorder. [provided by RefSeq, Apr 2016]